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The map label for this gene is pyrC

Identifier: 52785529

GI number: 52785529

Start: 1726821

End: 1728107

Strand: Direct

Name: pyrC

Synonym: BLi01770

Alternate gene names: 52785529

Gene position: 1726821-1728107 (Clockwise)

Preceding gene: 52785528

Following gene: 52785530

Centisome position: 40.89

GC content: 52.14

Gene sequence:

>1287_bases
ATGTCTTATTTAATTAAAAACGGCTTCATGCTCGACGAAAAAGGCGAAAAAGTACAGCGCGATATCAGGGTTGAAGGCGA
TGCAATCAGCGAGATCGGAAGCCTTGAGGCCGCTTCGGGGGAAACGGTCATCGATGCGGATGGACTTTTCGTCTCGCCAG
GGCTTGTTGACCTTCACGTTCACTTCAGAGAGCCGGGCGGCGAGAAGAAAGAAACGATCGAAACAGGTGCAAAAGCCGCA
GCGAGAGGCGGGTTTACAACTGTCGCCGCAATGCCGAATACCCGGCCTGTCCCCGATACGAAAGAGCAGATGGAGTGGCT
TGTCAACCGCATTGACGAAACGGCATCAGTGAGAGTGCTGCCGTATGCTTCAATCACCATCAGGCAAACAGGTCGGGAAA
TGACCGATTTCGAAGGGCTGAAAGACGCCGGCGCGTTCGCGTTTACCGATGACGGCGTCGGCGTTCAAACGGCGGGGATG
ATGTATGAAGCGATGAAAAAAGCGGCTTCGATTAATAAAGCGATCGTCGCTCACTGTGAAGACAATTCACTGATCTACGG
CGGCAGTGTACACGATGGGGAATTTGCCAAAGCCAACGGTTTAAACGGGATTCCTTCGGTGTGCGAAGCGGTTCACATCG
CAAGGGATGTGCTTCTTGCCGAAGCGGCAGGCTGCCATTACCACGTCTGTCATATCAGCACGAAGGAATCGGTCAGGGTC
GTCCGCGACGCCAAAAAAGCGGGAATCCGCGTGACGGCGGAAGTGACGCCGCACCATCTGCTCCTTTCAGACAGCGACAT
TCCCGGCCTTGATACGAATTATAAAATGAATCCGCCGCTTAGAAGTCCGGAAGACAGGGAAGCGCTCCTTGAGGGGCTTA
GAGACGGAACAATTGATTTTATCGCAACGGATCATGCCCCGCATACAGAAGAAGAAAAACAACAGACAATGAGCCTTGCG
CCTTTTGGAATTGTCGGCTTGGAGACGGCTTTTCCGCTCCTTTACACGCACTTTGTCAAAACAGGCAAATGGACGCTGAA
ACAGCTGCACGATTATATGACGGTCAAACCTTGCGAGGCGTTCGGGCTGCCGTACGGGAAGCTTGAAGCCGGACGGTCCG
CGGATATTACGCTCATTGATCTTGAACGCGAAGAAAAAATTGATAAAAGCACCTTTCTTTCTAAAGGGAAAAACACGCCG
TTTGACGGCATCAGCTGCTTCGGCTGGCCGGCTATGACAATGGCAAAGGGAAAACTCGTTTACCAAGAGGGGAGACTTGT
AAAATGA

Upstream 100 bases:

>100_bases
AGGATTTTTAAACAAATGGAAAACGGAGTATATATCAGAATGGCGGTTTTAAAAAGAGCGCTTCTAAACGGCGAAAACAA
AAAAAGGGGAGATCAGGCTT

Downstream 100 bases:

>100_bases
AAAGACGTCTTATATTAGAAAACGGAACGATTTTTGAAGGGGAAGCATTCGGCAGCCTGGAAAACACCATGGGAGAAGTC
GTCTTCAACACGGGGATGAC

Product: hypothetical protein

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSYLIKNGFMLDEKGEKVQRDIRVEGDAISEIGSLEAASGETVIDADGLFVSPGLVDLHVHFREPGGEKKETIETGAKAA
ARGGFTTVAAMPNTRPVPDTKEQMEWLVNRIDETASVRVLPYASITIRQTGREMTDFEGLKDAGAFAFTDDGVGVQTAGM
MYEAMKKAASINKAIVAHCEDNSLIYGGSVHDGEFAKANGLNGIPSVCEAVHIARDVLLAEAAGCHYHVCHISTKESVRV
VRDAKKAGIRVTAEVTPHHLLLSDSDIPGLDTNYKMNPPLRSPEDREALLEGLRDGTIDFIATDHAPHTEEEKQQTMSLA
PFGIVGLETAFPLLYTHFVKTGKWTLKQLHDYMTVKPCEAFGLPYGKLEAGRSADITLIDLEREEKIDKSTFLSKGKNTP
FDGISCFGWPAMTMAKGKLVYQEGRLVK

Sequences:

>Translated_428_residues
MSYLIKNGFMLDEKGEKVQRDIRVEGDAISEIGSLEAASGETVIDADGLFVSPGLVDLHVHFREPGGEKKETIETGAKAA
ARGGFTTVAAMPNTRPVPDTKEQMEWLVNRIDETASVRVLPYASITIRQTGREMTDFEGLKDAGAFAFTDDGVGVQTAGM
MYEAMKKAASINKAIVAHCEDNSLIYGGSVHDGEFAKANGLNGIPSVCEAVHIARDVLLAEAAGCHYHVCHISTKESVRV
VRDAKKAGIRVTAEVTPHHLLLSDSDIPGLDTNYKMNPPLRSPEDREALLEGLRDGTIDFIATDHAPHTEEEKQQTMSLA
PFGIVGLETAFPLLYTHFVKTGKWTLKQLHDYMTVKPCEAFGLPYGKLEAGRSADITLIDLEREEKIDKSTFLSKGKNTP
FDGISCFGWPAMTMAKGKLVYQEGRLVK
>Mature_427_residues
SYLIKNGFMLDEKGEKVQRDIRVEGDAISEIGSLEAASGETVIDADGLFVSPGLVDLHVHFREPGGEKKETIETGAKAAA
RGGFTTVAAMPNTRPVPDTKEQMEWLVNRIDETASVRVLPYASITIRQTGREMTDFEGLKDAGAFAFTDDGVGVQTAGMM
YEAMKKAASINKAIVAHCEDNSLIYGGSVHDGEFAKANGLNGIPSVCEAVHIARDVLLAEAAGCHYHVCHISTKESVRVV
RDAKKAGIRVTAEVTPHHLLLSDSDIPGLDTNYKMNPPLRSPEDREALLEGLRDGTIDFIATDHAPHTEEEKQQTMSLAP
FGIVGLETAFPLLYTHFVKTGKWTLKQLHDYMTVKPCEAFGLPYGKLEAGRSADITLIDLEREEKIDKSTFLSKGKNTPF
DGISCFGWPAMTMAKGKLVYQEGRLVK

Specific function: Involved In The Anaerobic Utilization Of Allantoin. [C]

COG id: COG0044

COG function: function code F; Dihydroorotase and related cyclic amidohydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI18105007, Length=381, Percent_Identity=32.0209973753281, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4503375, Length=450, Percent_Identity=23.5555555555556, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI19923821, Length=444, Percent_Identity=23.4234234234234, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4503051, Length=457, Percent_Identity=21.8818380743982, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI62422571, Length=457, Percent_Identity=21.8818380743982, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI4503377, Length=452, Percent_Identity=23.4513274336283, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI190194363, Length=455, Percent_Identity=22.1978021978022, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI4503379, Length=448, Percent_Identity=21.2053571428571, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786722, Length=455, Percent_Identity=28.5714285714286, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI87082175, Length=456, Percent_Identity=25.8771929824561, Blast_Score=125, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI193204318, Length=385, Percent_Identity=33.2467532467532, Blast_Score=160, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI71989490, Length=447, Percent_Identity=23.7136465324385, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17539558, Length=455, Percent_Identity=24.1758241758242, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI86575075, Length=442, Percent_Identity=21.4932126696833, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322218, Length=412, Percent_Identity=27.6699029126214, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24642586, Length=384, Percent_Identity=31.5104166666667, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18859883, Length=453, Percent_Identity=25.8278145695364, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRC_BACLD (Q65JU9)

Other databases:

- EMBL:   CP000002
- EMBL:   AE017333
- RefSeq:   YP_078943.1
- RefSeq:   YP_091358.1
- HSSP:   O66990
- ProteinModelPortal:   Q65JU9
- STRING:   Q65JU9
- EnsemblBacteria:   EBBACT00000054267
- EnsemblBacteria:   EBBACT00000061963
- GeneID:   3030921
- GeneID:   3097653
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01770
- KEGG:   bli:BL02274
- NMPDR:   fig|279010.5.peg.1712
- eggNOG:   COG0044
- GeneTree:   EBGT00050000001626
- HOGENOM:   HBG724623
- OMA:   PRVAEES
- ProtClustDB:   CLSK887279
- BioCyc:   BLIC279010-1:BLI01770-MONOMER
- BioCyc:   BLIC279010:BL02274-MONOMER
- HAMAP:   MF_00220_B
- InterPro:   IPR006680
- InterPro:   IPR004722
- InterPro:   IPR002195
- InterPro:   IPR011059
- TIGRFAMs:   TIGR00857

Pfam domain/function: PF01979 Amidohydro_1; SSF51338 Metalo_hydrolase

EC number: =3.5.2.3

Molecular weight: Translated: 46685; Mature: 46553

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLIKNGFMLDEKGEKVQRDIRVEGDAISEIGSLEAASGETVIDADGLFVSPGLVDLHV
CCEEECCCEEECCCCHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCEEECCCEEEEEE
HFREPGGEKKETIETGAKAAARGGFTTVAAMPNTRPVPDTKEQMEWLVNRIDETASVRVL
EEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
PYASITIRQTGREMTDFEGLKDAGAFAFTDDGVGVQTAGMMYEAMKKAASINKAIVAHCE
EEEEEEEECCCCHHHHHCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEC
DNSLIYGGSVHDGEFAKANGLNGIPSVCEAVHIARDVLLAEAAGCHYHVCHISTKESVRV
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHH
VRDAKKAGIRVTAEVTPHHLLLSDSDIPGLDTNYKMNPPLRSPEDREALLEGLRDGTIDF
HHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHCCCCEEE
IATDHAPHTEEEKQQTMSLAPFGIVGLETAFPLLYTHFVKTGKWTLKQLHDYMTVKPCEA
EEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCEECCHHH
FGLPYGKLEAGRSADITLIDLEREEKIDKSTFLSKGKNTPFDGISCFGWPAMTMAKGKLV
HCCCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHCCCCCCCCCEEECCCCHHHHHCCEEE
YQEGRLVK
EECCCCCC
>Mature Secondary Structure 
SYLIKNGFMLDEKGEKVQRDIRVEGDAISEIGSLEAASGETVIDADGLFVSPGLVDLHV
CEEECCCEEECCCCHHHHHHHCCCCHHHHHHCCCCCCCCCEEEECCCEEECCCEEEEEE
HFREPGGEKKETIETGAKAAARGGFTTVAAMPNTRPVPDTKEQMEWLVNRIDETASVRVL
EEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
PYASITIRQTGREMTDFEGLKDAGAFAFTDDGVGVQTAGMMYEAMKKAASINKAIVAHCE
EEEEEEEECCCCHHHHHCCCHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEC
DNSLIYGGSVHDGEFAKANGLNGIPSVCEAVHIARDVLLAEAAGCHYHVCHISTKESVRV
CCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHH
VRDAKKAGIRVTAEVTPHHLLLSDSDIPGLDTNYKMNPPLRSPEDREALLEGLRDGTIDF
HHHHHHCCEEEEEECCCCEEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHCCCCEEE
IATDHAPHTEEEKQQTMSLAPFGIVGLETAFPLLYTHFVKTGKWTLKQLHDYMTVKPCEA
EEECCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCHHHHHHHHHCEECCHHH
FGLPYGKLEAGRSADITLIDLEREEKIDKSTFLSKGKNTPFDGISCFGWPAMTMAKGKLV
HCCCCCCCCCCCCCCEEEEECCHHHHCCHHHHHHCCCCCCCCCEEECCCCHHHHHCCEEE
YQEGRLVK
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA