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The map label for this gene is pyrAA [H]

Identifier: 52785530

GI number: 52785530

Start: 1728104

End: 1729198

Strand: Direct

Name: pyrAA [H]

Synonym: BLi01771

Alternate gene names: 52785530

Gene position: 1728104-1729198 (Clockwise)

Preceding gene: 52785529

Following gene: 52785531

Centisome position: 40.92

GC content: 48.4

Gene sequence:

>1095_bases
ATGAAAAGACGTCTTATATTAGAAAACGGAACGATTTTTGAAGGGGAAGCATTCGGCAGCCTGGAAAACACCATGGGAGA
AGTCGTCTTCAACACGGGGATGACAGGCTATCAGGAAATCCTCTCAGACCCGTCATACTGCGGACAAATTGTCACCCTGA
CCTACCCTTTGATCGGGAACTACGGGATCAACAGAGACGACTTTGAATCCATCACCCCTTATGTAAAAGGGTTTATCGTA
AAGGAATTATGTCAACTTCCATCAAACTGGCGCTCTGCTTATACACTTGATGAATATTTAAAAATGAAAAACATCCCGGG
ACTCTCAGGGATAGACACGAGAAAGCTGACAAGAATGATCAGAACGGCAGGTACGCTGAAAGGGGTATTTGCCGGCCCTG
AAGAAGATGCTGGGGCTTTGGTTCAAAAGCTGAAAGAAACGAACCTTCCGACCGATCAGGTCAGCCAAGTATCAACAAAA
ACGGCATATCCGAGCCCTGGGAGAGGGAAGCGAATCGTGCTCGTCGATTTCGGAATGAAACACGGCATCTTAAGGGAGCT
GAACAAGCGCAAATGCGATGTGATCGTCGTTCCCCACAATATAACGGCAGAAGAAGTGATGCAGCTGAAGCCTGACGGCG
TAATGCTGTCAAACGGACCCGGAGATCCAACGGACGTACCGGAAGCGATTGCGATGATCCAAAACATTCTCGGCAAGGTT
CCGCTGTTCGGCATCTGTCTCGGCCATCAGCTGTTTGCCCTCGCCTGTGGAGCAAAGACGGAGAAAATGAAGTTCGGACA
CCGCGGTTCCAACCATCCGGTTAAAGAGCTTGAAACCGGCAGAGTATCAATTACGGCTCAAAACCACGGCTATACCGTAT
CGTCTGTTGAAAACACGCCGCTTGAAGTGACCCATGTGGCGATAAACGACGACACAATCGAAGGGCTTAAACATAAACAC
GAACCGGCTTTTACGGTTCAATACCATCCTGAAGCTTCACCGGGGCCGGAAGACGCAAACCATTTATTTGATCAATTTTT
AGACATGATTGAAACCACAGAGAAAGAAGGGGAAGCGGTATGCCTAAACGCGTAG

Upstream 100 bases:

>100_bases
AAGGGAAAAACACGCCGTTTGACGGCATCAGCTGCTTCGGCTGGCCGGCTATGACAATGGCAAAGGGAAAACTCGTTTAC
CAAGAGGGGAGACTTGTAAA

Downstream 100 bases:

>100_bases
ATATCAAAAAGATTTTGGTGATCGGATCGGGGCCGATTATTATCGGGCAGGCGGCGGAATTTGATTATGCAGGCACACAG
GCCTGCCTCGCTTTAAAAGA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKRRLILENGTIFEGEAFGSLENTMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGNYGINRDDFESITPYVKGFIV
KELCQLPSNWRSAYTLDEYLKMKNIPGLSGIDTRKLTRMIRTAGTLKGVFAGPEEDAGALVQKLKETNLPTDQVSQVSTK
TAYPSPGRGKRIVLVDFGMKHGILRELNKRKCDVIVVPHNITAEEVMQLKPDGVMLSNGPGDPTDVPEAIAMIQNILGKV
PLFGICLGHQLFALACGAKTEKMKFGHRGSNHPVKELETGRVSITAQNHGYTVSSVENTPLEVTHVAINDDTIEGLKHKH
EPAFTVQYHPEASPGPEDANHLFDQFLDMIETTEKEGEAVCLNA

Sequences:

>Translated_364_residues
MKRRLILENGTIFEGEAFGSLENTMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGNYGINRDDFESITPYVKGFIV
KELCQLPSNWRSAYTLDEYLKMKNIPGLSGIDTRKLTRMIRTAGTLKGVFAGPEEDAGALVQKLKETNLPTDQVSQVSTK
TAYPSPGRGKRIVLVDFGMKHGILRELNKRKCDVIVVPHNITAEEVMQLKPDGVMLSNGPGDPTDVPEAIAMIQNILGKV
PLFGICLGHQLFALACGAKTEKMKFGHRGSNHPVKELETGRVSITAQNHGYTVSSVENTPLEVTHVAINDDTIEGLKHKH
EPAFTVQYHPEASPGPEDANHLFDQFLDMIETTEKEGEAVCLNA
>Mature_364_residues
MKRRLILENGTIFEGEAFGSLENTMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGNYGINRDDFESITPYVKGFIV
KELCQLPSNWRSAYTLDEYLKMKNIPGLSGIDTRKLTRMIRTAGTLKGVFAGPEEDAGALVQKLKETNLPTDQVSQVSTK
TAYPSPGRGKRIVLVDFGMKHGILRELNKRKCDVIVVPHNITAEEVMQLKPDGVMLSNGPGDPTDVPEAIAMIQNILGKV
PLFGICLGHQLFALACGAKTEKMKFGHRGSNHPVKELETGRVSITAQNHGYTVSSVENTPLEVTHVAINDDTIEGLKHKH
EPAFTVQYHPEASPGPEDANHLFDQFLDMIETTEKEGEAVCLNA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=371, Percent_Identity=42.0485175202156, Blast_Score=270, Evalue=1e-72,
Organism=Homo sapiens, GI21361331, Length=376, Percent_Identity=36.968085106383, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI169790915, Length=376, Percent_Identity=36.968085106383, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1786215, Length=376, Percent_Identity=47.3404255319149, Blast_Score=323, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI193204318, Length=368, Percent_Identity=38.5869565217391, Blast_Score=249, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324878, Length=379, Percent_Identity=38.5224274406332, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=390, Percent_Identity=35.6410256410256, Blast_Score=239, Evalue=4e-64,
Organism=Drosophila melanogaster, GI45555749, Length=381, Percent_Identity=38.8451443569554, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24642586, Length=378, Percent_Identity=39.1534391534392, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40017; Mature: 40017

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRLILENGTIFEGEAFGSLENTMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGN
CCCEEEECCCCEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEEEEEEHHHCC
YGINRDDFESITPYVKGFIVKELCQLPSNWRSAYTLDEYLKMKNIPGLSGIDTRKLTRMI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RTAGTLKGVFAGPEEDAGALVQKLKETNLPTDQVSQVSTKTAYPSPGRGKRIVLVDFGMK
HHHHHHCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
HGILRELNKRKCDVIVVPHNITAEEVMQLKPDGVMLSNGPGDPTDVPEAIAMIQNILGKV
HHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
PLFGICLGHQLFALACGAKTEKMKFGHRGSNHPVKELETGRVSITAQNHGYTVSSVENTP
CHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHCCCEEEEEECCCCEEEECCCCCC
LEVTHVAINDDTIEGLKHKHEPAFTVQYHPEASPGPEDANHLFDQFLDMIETTEKEGEAV
EEEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
CLNA
EECC
>Mature Secondary Structure
MKRRLILENGTIFEGEAFGSLENTMGEVVFNTGMTGYQEILSDPSYCGQIVTLTYPLIGN
CCCEEEECCCCEECCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHCCEEEEEEEHHHCC
YGINRDDFESITPYVKGFIVKELCQLPSNWRSAYTLDEYLKMKNIPGLSGIDTRKLTRMI
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RTAGTLKGVFAGPEEDAGALVQKLKETNLPTDQVSQVSTKTAYPSPGRGKRIVLVDFGMK
HHHHHHCEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCH
HGILRELNKRKCDVIVVPHNITAEEVMQLKPDGVMLSNGPGDPTDVPEAIAMIQNILGKV
HHHHHHHHCCCCCEEEECCCCCHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCC
PLFGICLGHQLFALACGAKTEKMKFGHRGSNHPVKELETGRVSITAQNHGYTVSSVENTP
CHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHCCCEEEEEECCCCEEEECCCCCC
LEVTHVAINDDTIEGLKHKHEPAFTVQYHPEASPGPEDANHLFDQFLDMIETTEKEGEAV
EEEEEEEECCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEE
CLNA
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1709162; 9384377 [H]