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The map label for this gene is mtlA [H]

Identifier: 52784325

GI number: 52784325

Start: 510888

End: 512312

Strand: Direct

Name: mtlA [H]

Synonym: BLi00505

Alternate gene names: 52784325

Gene position: 510888-512312 (Clockwise)

Preceding gene: 52784324

Following gene: 52784326

Centisome position: 12.1

GC content: 49.12

Gene sequence:

>1425_bases
ATGGAAGATAGACAAAATCAAGATTTCAAAGTAAAGATTCAACGTTTCGGAAGCTATTTGAGCGGAATGATCATGCCGAA
TATCGGTGCATTTATCGCATGGGGGATCATTACCGCATTGTTTATTCCGACGGGATGGATGCCGAATGAAGCGCTTGCTT
CATTGGTTGATCCAATGATCAAATTTTTGCTGCCGCTCTTGATTGGATACACGGGCGGTAAGATGGTGTACGATCACCGC
GGAGGCGTCGTCGGTGCGACTGCAGCGATCGGCGTTATCGTCGGCGCGGACATTCCGATGTTCCTCGGCGCGATGATCAT
GGGGCCGCTGGGCGGCTACCTGATGAAACTGACGGACAGGCTTTTCAAAGATAAGGTCCGTTCCGGATTTGAAATGCTGA
TCAACAACTTTACAGCCGGAATTCTCGGCTTCATTCTGGCGGTGCTCGGCTTTCTTGCAATCGGTCCGGTGGTGCTCGGC
TTAAACAAAACATTGGCGGCCGGCGTGGAAGTGATCGTCAATGCGAATCTGCTTCCGCTCGCAAGTATTTTTGTAGAGCC
TGCGAAAGTTCTTTTCCTTAACAATGCGATCAACCACGGTATTTTGAGCCCGATTGGTATTGAGCAGGCCGCCCAGACAG
GAAAATCAATTTTATTCCTTTTAGAGGCGAACCCAGGCCCTGGTCTCGGGATTCTTTTAGCTTACATGATTTTCGGAAGA
GGCACATCAAAGCAGACCGCTCCGGGCGCTGCAGTGATCCATTTCCTAGGCGGTATTCACGAAATTTATTTCCCTTACAT
TCTGATGAAACCGCTGTTAATCCTGGCTGCAATAGCGGGCGGAGCGAGCGGTGTGCTGACGTTTTCCATCTTTAATGCGG
GGCTTGCGGCTGTTCCTTCACCTGGAAGTATTATTGCGGTGCTGGCGATGACGCCGAAAGGCAACCACATCGGCGTGATC
CTTGGTGTTCTCGTCGCAGCAGTCGTTTCATTCATTGTAGCTTCTTTGATTTTGAAAACGTCTAAAGCCGGCGAAGAAGA
TTTGACGGCCGCGGCGGAAAAAATGCAGCAAATGAAAGGCAAGAAGAGCCAGGCCGTTTCTGCATTGACTTCGGCTGACG
AAGCGGAAGAATCAGCGGCTCCGTACGAAAATGTCAGCAAGATCATCTTCGCGTGTGATGCCGGAATGGGCTCCAGTGCG
ATGGGGGCATCAATTTTGAAAAACAAAGTCAAAAAAGCAGAACTCGATATCGACGTATCCAATACATCGATCAATAATCT
GCCGGAAGATGCCGATATCGTCATCACGCATAAAGACTTAACGGAACGCGCAAAAGCGAAGCTTCCAAGCGCCAGACACA
TATCGGTGGAAAACTTCCTTAACAGTCCAAAATACGACGAACTGATCAATCAGCTTAAAAAGTAA

Upstream 100 bases:

>100_bases
AGTCATCTGTAAGCGTTTTAATATAGAAACAGGGAGCCGACATTATTCGACATCATTTTTTTATCACAGGCTTTAAAGGA
CAACAAAGGAGGGTTATGAA

Downstream 100 bases:

>100_bases
AGTTTGAGAACCCGTGAGCCTGAAAATCAGGCTCACACAACATGTATGAAAGAGAGTGATTTCCCAATGAAACCAGTACT
TGCAAAAGAAAACATTCATC

Product: hypothetical protein

Products: NA

Alternate protein names: EIICB-Mtl; EII-Mtl; Mannitol permease IIC component; PTS system mannitol-specific EIIC component; Mannitol-specific phosphotransferase enzyme IIB component; PTS system mannitol-specific EIIB component [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MEDRQNQDFKVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWMPNEALASLVDPMIKFLLPLLIGYTGGKMVYDHR
GGVVGATAAIGVIVGADIPMFLGAMIMGPLGGYLMKLTDRLFKDKVRSGFEMLINNFTAGILGFILAVLGFLAIGPVVLG
LNKTLAAGVEVIVNANLLPLASIFVEPAKVLFLNNAINHGILSPIGIEQAAQTGKSILFLLEANPGPGLGILLAYMIFGR
GTSKQTAPGAAVIHFLGGIHEIYFPYILMKPLLILAAIAGGASGVLTFSIFNAGLAAVPSPGSIIAVLAMTPKGNHIGVI
LGVLVAAVVSFIVASLILKTSKAGEEDLTAAAEKMQQMKGKKSQAVSALTSADEAEESAAPYENVSKIIFACDAGMGSSA
MGASILKNKVKKAELDIDVSNTSINNLPEDADIVITHKDLTERAKAKLPSARHISVENFLNSPKYDELINQLKK

Sequences:

>Translated_474_residues
MEDRQNQDFKVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWMPNEALASLVDPMIKFLLPLLIGYTGGKMVYDHR
GGVVGATAAIGVIVGADIPMFLGAMIMGPLGGYLMKLTDRLFKDKVRSGFEMLINNFTAGILGFILAVLGFLAIGPVVLG
LNKTLAAGVEVIVNANLLPLASIFVEPAKVLFLNNAINHGILSPIGIEQAAQTGKSILFLLEANPGPGLGILLAYMIFGR
GTSKQTAPGAAVIHFLGGIHEIYFPYILMKPLLILAAIAGGASGVLTFSIFNAGLAAVPSPGSIIAVLAMTPKGNHIGVI
LGVLVAAVVSFIVASLILKTSKAGEEDLTAAAEKMQQMKGKKSQAVSALTSADEAEESAAPYENVSKIIFACDAGMGSSA
MGASILKNKVKKAELDIDVSNTSINNLPEDADIVITHKDLTERAKAKLPSARHISVENFLNSPKYDELINQLKK
>Mature_474_residues
MEDRQNQDFKVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWMPNEALASLVDPMIKFLLPLLIGYTGGKMVYDHR
GGVVGATAAIGVIVGADIPMFLGAMIMGPLGGYLMKLTDRLFKDKVRSGFEMLINNFTAGILGFILAVLGFLAIGPVVLG
LNKTLAAGVEVIVNANLLPLASIFVEPAKVLFLNNAINHGILSPIGIEQAAQTGKSILFLLEANPGPGLGILLAYMIFGR
GTSKQTAPGAAVIHFLGGIHEIYFPYILMKPLLILAAIAGGASGVLTFSIFNAGLAAVPSPGSIIAVLAMTPKGNHIGVI
LGVLVAAVVSFIVASLILKTSKAGEEDLTAAAEKMQQMKGKKSQAVSALTSADEAEESAAPYENVSKIIFACDAGMGSSA
MGASILKNKVKKAELDIDVSNTSINNLPEDADIVITHKDLTERAKAKLPSARHISVENFLNSPKYDELINQLKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2213

COG function: function code G; Phosphotransferase system, mannitol-specific IIBC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=469, Percent_Identity=62.8997867803838, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI1789301, Length=468, Percent_Identity=52.5641025641026, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004718 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49942; Mature: 49942

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDRQNQDFKVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWMPNEALASLVDPMI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KFLLPLLIGYTGGKMVYDHRGGVVGATAAIGVIVGADIPMFLGAMIMGPLGGYLMKLTDR
HHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LFKDKVRSGFEMLINNFTAGILGFILAVLGFLAIGPVVLGLNKTLAAGVEVIVNANLLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHH
ASIFVEPAKVLFLNNAINHGILSPIGIEQAAQTGKSILFLLEANPGPGLGILLAYMIFGR
HHHHHCHHHHEEECCHHCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCC
GTSKQTAPGAAVIHFLGGIHEIYFPYILMKPLLILAAIAGGASGVLTFSIFNAGLAAVPS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCCCCC
PGSIIAVLAMTPKGNHIGVILGVLVAAVVSFIVASLILKTSKAGEEDLTAAAEKMQQMKG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
KKSQAVSALTSADEAEESAAPYENVSKIIFACDAGMGSSAMGASILKNKVKKAELDIDVS
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHEEEEEC
NTSINNLPEDADIVITHKDLTERAKAKLPSARHISVENFLNSPKYDELINQLKK
CCCCCCCCCCCCEEEECHHHHHHHHHCCCCCCEEEHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MEDRQNQDFKVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWMPNEALASLVDPMI
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KFLLPLLIGYTGGKMVYDHRGGVVGATAAIGVIVGADIPMFLGAMIMGPLGGYLMKLTDR
HHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LFKDKVRSGFEMLINNFTAGILGFILAVLGFLAIGPVVLGLNKTLAAGVEVIVNANLLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHH
ASIFVEPAKVLFLNNAINHGILSPIGIEQAAQTGKSILFLLEANPGPGLGILLAYMIFGR
HHHHHCHHHHEEECCHHCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHCC
GTSKQTAPGAAVIHFLGGIHEIYFPYILMKPLLILAAIAGGASGVLTFSIFNAGLAAVPS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCCCCC
PGSIIAVLAMTPKGNHIGVILGVLVAAVVSFIVASLILKTSKAGEEDLTAAAEKMQQMKG
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
KKSQAVSALTSADEAEESAAPYENVSKIIFACDAGMGSSAMGASILKNKVKKAELDIDVS
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHEEEEEC
NTSINNLPEDADIVITHKDLTERAKAKLPSARHISVENFLNSPKYDELINQLKK
CCCCCCCCCCCCEEEECHHHHHHHHHCCCCCCEEEHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]