Click here to switch to the map view.

The map label for this gene is mtlF [H]

Identifier: 52784326

GI number: 52784326

Start: 512379

End: 512819

Strand: Direct

Name: mtlF [H]

Synonym: BLi00506

Alternate gene names: 52784326

Gene position: 512379-512819 (Clockwise)

Preceding gene: 52784325

Following gene: 52784327

Centisome position: 12.13

GC content: 46.71

Gene sequence:

>441_bases
ATGAAACCAGTACTTGCAAAAGAAAACATTCATCTCAATCAATCTGCCGCAACGCAGGAAGAAGCGATCAGACTGGCCGG
AAAGGCGCTGGTTGACGCCGGCTATGTTACCGCGGATTATATCGAGAAAATGTTGGAGAGGGAAGGCCTCACTTCCACTT
ACATGGGGAACGCAATCGCCATTCCGCATGGTACGGAAGACAGCAAGCAGGATGTTCTTCATTCCGGAATATCCGTTATC
CAGCTTCCGGAAGGTGTCGAATACGGCGATGGCAATATTGCTAAAGTGATCTTTGGCATTGCCGGAAAAAATGATGAGCA
TTTGGAGATTTTGTCAAAGATTGCGATTGTCTGCTCAGAAGCGGACAATGTCGAACGGATCATCAATGCGAAAAATGAAG
ACGAGTTAATGGCGGTTTTTGAAGAAGGTGACCAGGTATGA

Upstream 100 bases:

>100_bases
ATACGACGAACTGATCAATCAGCTTAAAAAGTAAAGTTTGAGAACCCGTGAGCCTGAAAATCAGGCTCACACAACATGTA
TGAAAGAGAGTGATTTCCCA

Downstream 100 bases:

>100_bases
TTGCACTGCATTTCGGTGCGGGGAACATCGGCCGCGGATTTATCGGAGCCTTGCTCTGCAAATCGGGGTATGATGTCGTG
TTCGCCGATGTAAATGATGC

Product: hypothetical protein

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MKPVLAKENIHLNQSAATQEEAIRLAGKALVDAGYVTADYIEKMLEREGLTSTYMGNAIAIPHGTEDSKQDVLHSGISVI
QLPEGVEYGDGNIAKVIFGIAGKNDEHLEILSKIAIVCSEADNVERIINAKNEDELMAVFEEGDQV

Sequences:

>Translated_146_residues
MKPVLAKENIHLNQSAATQEEAIRLAGKALVDAGYVTADYIEKMLEREGLTSTYMGNAIAIPHGTEDSKQDVLHSGISVI
QLPEGVEYGDGNIAKVIFGIAGKNDEHLEILSKIAIVCSEADNVERIINAKNEDELMAVFEEGDQV
>Mature_146_residues
MKPVLAKENIHLNQSAATQEEAIRLAGKALVDAGYVTADYIEKMLEREGLTSTYMGNAIAIPHGTEDSKQDVLHSGISVI
QLPEGVEYGDGNIAKVIFGIAGKNDEHLEILSKIAIVCSEADNVERIINAKNEDELMAVFEEGDQV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1790027, Length=135, Percent_Identity=42.962962962963, Blast_Score=126, Evalue=6e-31,
Organism=Escherichia coli, GI1788494, Length=136, Percent_Identity=45.5882352941176, Blast_Score=124, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 15809; Mature: 15809

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVLAKENIHLNQSAATQEEAIRLAGKALVDAGYVTADYIEKMLEREGLTSTYMGNAIA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHCCCEEE
IPHGTEDSKQDVLHSGISVIQLPEGVEYGDGNIAKVIFGIAGKNDEHLEILSKIAIVCSE
CCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCC
ADNVERIINAKNEDELMAVFEEGDQV
CCCHHHHHCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MKPVLAKENIHLNQSAATQEEAIRLAGKALVDAGYVTADYIEKMLEREGLTSTYMGNAIA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHCCCEEE
IPHGTEDSKQDVLHSGISVIQLPEGVEYGDGNIAKVIFGIAGKNDEHLEILSKIAIVCSE
CCCCCCCHHHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCC
ADNVERIINAKNEDELMAVFEEGDQV
CCCHHHHHCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]