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The map label for this gene is nasD [H]

Identifier: 52784304

GI number: 52784304

Start: 489769

End: 492189

Strand: Direct

Name: nasD [H]

Synonym: BLi00484

Alternate gene names: 52784304

Gene position: 489769-492189 (Clockwise)

Preceding gene: 52784303

Following gene: 52784305

Centisome position: 11.6

GC content: 48.45

Gene sequence:

>2421_bases
GTGAAAAAAAAGCAGCTTGTCCTTGTAGGGAACGGTATGGCCGGGGTCAGGGCCATTGAAGAAATTCTTAAAATTTCAAA
CGAAGAGTTTCAGATCACGATTTTCGGCAGCGAACCGCACCCAAACTATAACCGGATTCTTCTTTCCAAAGTGCTTCAAG
GAGATACGGATGTTAAGGACATTACACTGAATGATTGGGATTGGTATGAAGAAAACGGCATTCAGCTGTATACAGGTGAA
GAAGTCGTTAAAGTTGATCCGGAGTCCAAAACCGTCATCACGGATACGGGAAGAGTCCAGCCGTATGATGAACTGATTTT
GGCGACAGGTTCTCTCCCTTTTATTCTGCCATTGCCCGGAGCGGATAAAGAAGGTGTAACAGCTTTCCGTGATATCAAGG
ATACGGATATCATGCTCGAAGCTTCCAAAACGTACAAAAAAGCAGCAGTTATCGGCGGCGGGCTGCTGGGACTGGAAGCA
GCGCGCGGACTTCTCAACCTTGGCATGGATGTTACCGTTGTCCACCTCGCGCCATATTTGATGGAGCGTCAGCTTGATGC
AGCCGCAGGACGGCTTTTGCAAAAAGAGCTGGAAAAGCAGGGCATGAAATTTTTACTGGAAAAGCAGACCGAAGAAATTT
ACGGAGAAACACGTGTCGAAGGCTTAAAATTCAAAGACGGCTCAACGCTTGAGGCCGATTTGGTCGTCATGGCGGTCGGA
ATCAAGCCGAACGTTCAGCTCGGCAAAGACTGCGGCCTTCCGGTAAACAGAGGAATCGTCGTCAATGATTACATGGAGAC
CGAAGTTCCCAACATTTACGCTGTCGGAGAATGCGCGGAGCATAGGGGCATTGCCTACGGACTTGTCGCGCCTCTTTATG
AACAGGCAAAAGTGCTTGCCAAAAAAATCTGCGGCGTTGAAACGGCTCCGTATGAAGGATCTGTTTTATCTACCCAGCTG
AAGGTTTCCGGTGTTGAGGTCTTTTCTGCCGGTGACTTTAATGAGGATGACGGAGAAGACAAAAAAGCGCTCAAAGTGTT
TGACGAACAGGACGGAATCTACAAAAAAATCGTTCTGCGCGGCAATCAGGTGATCGGCGCCGTACTGTTCGGAGACAGCA
GCGAAGGAAACAGGCTGTTTTCCATGATTCAAAAAGGAGCGGATATTTCTGATACTTCAAAAATTTCAATCTTGCAGCCT
TTGAATCAGGAAGAAGGCATGAGCTTGACCGCTGCGATGAGCGACGATGAGATCGTGTGCGGCTGCAACGGCGTTTCGAA
AGGAATGATCATTCAGGCGATCAAAGAGCAGGGATGCACATCGACGGATGAAATTAAAGCATGCACAGGCGCATCGCGCT
CTTGCGGAGGATGTAAGCCGCTTGTCGCAGAGATTCTCCAGCATACGCTCGGCTCAGAATTTGATGCTTCAGCACAAAAA
GAAGCGATTTGCGGATGTACGGACCTTTCAAGAGACGAACTCGTGGCCGAAATCAGAGAAAAAGGGCTGACCCATACAAA
AGAAGTCATGAACGTGCTCGGCTGGAAAACGGCGGAAGGCTGTTCCAAATGCCGCCCTGCGCTTAACTACTACCTCGGCA
TGATCAATCCGAAGGAATATGAAGATGAAAGAGAATCCCGCTTCGTCAATGAACGGATGCACGCCAACATTCAAAAAGAC
GGCACATATTCAGTCGTACCGCGGATGTACGGCGGGGTCACCAATTCGAATGACTTGCGGCGCATCGCCGATGTCGTCGA
CAAATATGAAATTCCTCTAGTGAAAATGACCGGCGGCCAGCGGATCGATCTGATCGGCGTCAAAAAAGAGGATCTTCCGA
AAGTATGGGCTGATCTTGACATGCCTTCTGGATTTGCTTACGGAAAAACGCTCAGAACCGTAAAAACGTGCGTAGGCGAA
CAGTTCTGCCGCTTCGGAACGCAAGATTCAATGGCTCTCGGAATTGACCTTGAGAAAAAATTTGAAGGGCTCTACACGCC
TCACAAAATTAAAATGGCGGTTTCCGCTTGTCCGAGAAACTGTGCTGAATCCGGCATTAAAGATATCGGCGTTGTCGGCA
TCGACGGCGGCTGGGAAATTTACGTCGGCGGAAACGGAGGCACACATCTGCGTGCAGGCGATCTGTTAATGAAAGTCAAA
ACGGCTGAAGAAGTCATCGAAATCACAGGCGCATATCTCCAATACTATCGTGAAACAGCCAACTATTTAGAGCGGACATC
TGCATGGATCGAACGTGTCGGCTTGTCCCACGTCCAATCCGTATTGAATGATAAAGAAAAACGCAAAGAGCTGAATGACC
GCATCAATCAAGCGCTGTCTGTCCATAAAGACCCGTGGAAAGACTTTTTGGAAGACGGAAAGACAACAAGACAGCTATTC
GAAAATGTTGTCACTTCCTAA

Upstream 100 bases:

>100_bases
GTTTACAAAATTAACTATAATTTGTGATAGTTTTAAAGATGTAAATAGAATACTTGTTTAGGGCGTATAGTGAATTGTTT
TGAAAAAGGGGAGGATCATA

Downstream 100 bases:

>100_bases
AATGAAAAACCAACTTGAGGAGGAATTCACATGGTGAACAAGAACTTAACAAAGGTGTTTGTTTCGAAGATCGGGGATTT
GCCGAATCAGCTTGGGAAGA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 806; Mature: 806

Protein sequence:

>806_residues
MKKKQLVLVGNGMAGVRAIEEILKISNEEFQITIFGSEPHPNYNRILLSKVLQGDTDVKDITLNDWDWYEENGIQLYTGE
EVVKVDPESKTVITDTGRVQPYDELILATGSLPFILPLPGADKEGVTAFRDIKDTDIMLEASKTYKKAAVIGGGLLGLEA
ARGLLNLGMDVTVVHLAPYLMERQLDAAAGRLLQKELEKQGMKFLLEKQTEEIYGETRVEGLKFKDGSTLEADLVVMAVG
IKPNVQLGKDCGLPVNRGIVVNDYMETEVPNIYAVGECAEHRGIAYGLVAPLYEQAKVLAKKICGVETAPYEGSVLSTQL
KVSGVEVFSAGDFNEDDGEDKKALKVFDEQDGIYKKIVLRGNQVIGAVLFGDSSEGNRLFSMIQKGADISDTSKISILQP
LNQEEGMSLTAAMSDDEIVCGCNGVSKGMIIQAIKEQGCTSTDEIKACTGASRSCGGCKPLVAEILQHTLGSEFDASAQK
EAICGCTDLSRDELVAEIREKGLTHTKEVMNVLGWKTAEGCSKCRPALNYYLGMINPKEYEDERESRFVNERMHANIQKD
GTYSVVPRMYGGVTNSNDLRRIADVVDKYEIPLVKMTGGQRIDLIGVKKEDLPKVWADLDMPSGFAYGKTLRTVKTCVGE
QFCRFGTQDSMALGIDLEKKFEGLYTPHKIKMAVSACPRNCAESGIKDIGVVGIDGGWEIYVGGNGGTHLRAGDLLMKVK
TAEEVIEITGAYLQYYRETANYLERTSAWIERVGLSHVQSVLNDKEKRKELNDRINQALSVHKDPWKDFLEDGKTTRQLF
ENVVTS

Sequences:

>Translated_806_residues
MKKKQLVLVGNGMAGVRAIEEILKISNEEFQITIFGSEPHPNYNRILLSKVLQGDTDVKDITLNDWDWYEENGIQLYTGE
EVVKVDPESKTVITDTGRVQPYDELILATGSLPFILPLPGADKEGVTAFRDIKDTDIMLEASKTYKKAAVIGGGLLGLEA
ARGLLNLGMDVTVVHLAPYLMERQLDAAAGRLLQKELEKQGMKFLLEKQTEEIYGETRVEGLKFKDGSTLEADLVVMAVG
IKPNVQLGKDCGLPVNRGIVVNDYMETEVPNIYAVGECAEHRGIAYGLVAPLYEQAKVLAKKICGVETAPYEGSVLSTQL
KVSGVEVFSAGDFNEDDGEDKKALKVFDEQDGIYKKIVLRGNQVIGAVLFGDSSEGNRLFSMIQKGADISDTSKISILQP
LNQEEGMSLTAAMSDDEIVCGCNGVSKGMIIQAIKEQGCTSTDEIKACTGASRSCGGCKPLVAEILQHTLGSEFDASAQK
EAICGCTDLSRDELVAEIREKGLTHTKEVMNVLGWKTAEGCSKCRPALNYYLGMINPKEYEDERESRFVNERMHANIQKD
GTYSVVPRMYGGVTNSNDLRRIADVVDKYEIPLVKMTGGQRIDLIGVKKEDLPKVWADLDMPSGFAYGKTLRTVKTCVGE
QFCRFGTQDSMALGIDLEKKFEGLYTPHKIKMAVSACPRNCAESGIKDIGVVGIDGGWEIYVGGNGGTHLRAGDLLMKVK
TAEEVIEITGAYLQYYRETANYLERTSAWIERVGLSHVQSVLNDKEKRKELNDRINQALSVHKDPWKDFLEDGKTTRQLF
ENVVTS
>Mature_806_residues
MKKKQLVLVGNGMAGVRAIEEILKISNEEFQITIFGSEPHPNYNRILLSKVLQGDTDVKDITLNDWDWYEENGIQLYTGE
EVVKVDPESKTVITDTGRVQPYDELILATGSLPFILPLPGADKEGVTAFRDIKDTDIMLEASKTYKKAAVIGGGLLGLEA
ARGLLNLGMDVTVVHLAPYLMERQLDAAAGRLLQKELEKQGMKFLLEKQTEEIYGETRVEGLKFKDGSTLEADLVVMAVG
IKPNVQLGKDCGLPVNRGIVVNDYMETEVPNIYAVGECAEHRGIAYGLVAPLYEQAKVLAKKICGVETAPYEGSVLSTQL
KVSGVEVFSAGDFNEDDGEDKKALKVFDEQDGIYKKIVLRGNQVIGAVLFGDSSEGNRLFSMIQKGADISDTSKISILQP
LNQEEGMSLTAAMSDDEIVCGCNGVSKGMIIQAIKEQGCTSTDEIKACTGASRSCGGCKPLVAEILQHTLGSEFDASAQK
EAICGCTDLSRDELVAEIREKGLTHTKEVMNVLGWKTAEGCSKCRPALNYYLGMINPKEYEDERESRFVNERMHANIQKD
GTYSVVPRMYGGVTNSNDLRRIADVVDKYEIPLVKMTGGQRIDLIGVKKEDLPKVWADLDMPSGFAYGKTLRTVKTCVGE
QFCRFGTQDSMALGIDLEKKFEGLYTPHKIKMAVSACPRNCAESGIKDIGVVGIDGGWEIYVGGNGGTHLRAGDLLMKVK
TAEEVIEITGAYLQYYRETANYLERTSAWIERVGLSHVQSVLNDKEKRKELNDRINQALSVHKDPWKDFLEDGKTTRQLF
ENVVTS

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=29.7101449275362, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI65787454, Length=275, Percent_Identity=29.8181818181818, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=29.7101449275362, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI22202629, Length=256, Percent_Identity=27.34375, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI4757732, Length=256, Percent_Identity=27.34375, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1789765, Length=784, Percent_Identity=36.4795918367347, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1789065, Length=333, Percent_Identity=26.1261261261261, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1788892, Length=282, Percent_Identity=28.7234042553192, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1787352, Length=305, Percent_Identity=27.2131147540984, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=406, Percent_Identity=23.3990147783251, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI32565766, Length=256, Percent_Identity=28.90625, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281359715, Length=326, Percent_Identity=27.6073619631902, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281359713, Length=326, Percent_Identity=27.6073619631902, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639250, Length=326, Percent_Identity=27.6073619631902, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18543267, Length=326, Percent_Identity=27.6073619631902, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639252, Length=326, Percent_Identity=27.6073619631902, Blast_Score=118, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639257, Length=326, Percent_Identity=27.6073619631902, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24585130, Length=284, Percent_Identity=24.2957746478873, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI28573993, Length=317, Percent_Identity=26.1829652996845, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24581020, Length=316, Percent_Identity=25.6329113924051, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 88670; Mature: 88670

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKQLVLVGNGMAGVRAIEEILKISNEEFQITIFGSEPHPNYNRILLSKVLQGDTDVKD
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCE
ITLNDWDWYEENGIQLYTGEEVVKVDPESKTVITDTGRVQPYDELILATGSLPFILPLPG
EEECCCCCCCCCCEEEEECCEEEEECCCCCEEEECCCCCCCHHHHEEECCCCCEEEECCC
ADKEGVTAFRDIKDTDIMLEASKTYKKAAVIGGGLLGLEAARGLLNLGMDVTVVHLAPYL
CCCCCCHHHHCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
MERQLDAAAGRLLQKELEKQGMKFLLEKQTEEIYGETRVEGLKFKDGSTLEADLVVMAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEECCCCCCEEEEEEEEEC
IKPNVQLGKDCGLPVNRGIVVNDYMETEVPNIYAVGECAEHRGIAYGLVAPLYEQAKVLA
CCCCCCCCCCCCCCCCCCEEEECHHHCCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHH
KKICGVETAPYEGSVLSTQLKVSGVEVFSAGDFNEDDGEDKKALKVFDEQDGIYKKIVLR
HHHCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEHHEEE
GNQVIGAVLFGDSSEGNRLFSMIQKGADISDTSKISILQPLNQEEGMSLTAAMSDDEIVC
CCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCEEE
GCNGVSKGMIIQAIKEQGCTSTDEIKACTGASRSCGGCKPLVAEILQHTLGSEFDASAQK
ECCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
EAICGCTDLSRDELVAEIREKGLTHTKEVMNVLGWKTAEGCSKCRPALNYYLGMINPKEY
HHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
EDERESRFVNERMHANIQKDGTYSVVPRMYGGVTNSNDLRRIADVVDKYEIPLVKMTGGQ
CHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
RIDLIGVKKEDLPKVWADLDMPSGFAYGKTLRTVKTCVGEQFCRFGTQDSMALGIDLEKK
EEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHH
FEGLYTPHKIKMAVSACPRNCAESGIKDIGVVGIDGGWEIYVGGNGGTHLRAGDLLMKVK
HCCCCCCHHHHHHHHHCCHHHHHCCCCCCEEEEECCCEEEEECCCCCCEEECCCEEEEHH
TAEEVIEITGAYLQYYRETANYLERTSAWIERVGLSHVQSVLNDKEKRKELNDRINQALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VHKDPWKDFLEDGKTTRQLFENVVTS
CCCCHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKQLVLVGNGMAGVRAIEEILKISNEEFQITIFGSEPHPNYNRILLSKVLQGDTDVKD
CCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCE
ITLNDWDWYEENGIQLYTGEEVVKVDPESKTVITDTGRVQPYDELILATGSLPFILPLPG
EEECCCCCCCCCCEEEEECCEEEEECCCCCEEEECCCCCCCHHHHEEECCCCCEEEECCC
ADKEGVTAFRDIKDTDIMLEASKTYKKAAVIGGGLLGLEAARGLLNLGMDVTVVHLAPYL
CCCCCCHHHHCCCCCCEEEECHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEHHHHHHHH
MERQLDAAAGRLLQKELEKQGMKFLLEKQTEEIYGETRVEGLKFKDGSTLEADLVVMAVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCEEECCCCCCEEEEEEEEEC
IKPNVQLGKDCGLPVNRGIVVNDYMETEVPNIYAVGECAEHRGIAYGLVAPLYEQAKVLA
CCCCCCCCCCCCCCCCCCEEEECHHHCCCCCEEEECCHHHHCCCCHHHHHHHHHHHHHHH
KKICGVETAPYEGSVLSTQLKVSGVEVFSAGDFNEDDGEDKKALKVFDEQDGIYKKIVLR
HHHCCCCCCCCCCCEEEEEEEECCEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEHHEEE
GNQVIGAVLFGDSSEGNRLFSMIQKGADISDTSKISILQPLNQEEGMSLTAAMSDDEIVC
CCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEEEECCCCCEEE
GCNGVSKGMIIQAIKEQGCTSTDEIKACTGASRSCGGCKPLVAEILQHTLGSEFDASAQK
ECCCCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCH
EAICGCTDLSRDELVAEIREKGLTHTKEVMNVLGWKTAEGCSKCRPALNYYLGMINPKEY
HHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHH
EDERESRFVNERMHANIQKDGTYSVVPRMYGGVTNSNDLRRIADVVDKYEIPLVKMTGGQ
CHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCC
RIDLIGVKKEDLPKVWADLDMPSGFAYGKTLRTVKTCVGEQFCRFGTQDSMALGIDLEKK
EEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHH
FEGLYTPHKIKMAVSACPRNCAESGIKDIGVVGIDGGWEIYVGGNGGTHLRAGDLLMKVK
HCCCCCCHHHHHHHHHCCHHHHHCCCCCCEEEEECCCEEEEECCCCCCEEECCCEEEEHH
TAEEVIEITGAYLQYYRETANYLERTSAWIERVGLSHVQSVLNDKEKRKELNDRINQALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VHKDPWKDFLEDGKTTRQLFENVVTS
CCCCHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]