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The map label for this gene is yjgC [H]

Identifier: 51894430

GI number: 51894430

Start: 3520591

End: 3520995

Strand: Reverse

Name: yjgC [H]

Synonym: STH3296

Alternate gene names: 51894430

Gene position: 3520995-3520591 (Counterclockwise)

Preceding gene: 51894431

Following gene: 51894429

Centisome position: 98.73

GC content: 63.95

Gene sequence:

>405_bases
GTGAAGCAAGTCCTGACCACCTGTCCCTACTGCGGAACGGGCTGCACGTTCTACCTGAACGTGGAAGGGGACCAGGTCGT
GGGCGTGACGCCCAACGACGACCCCCGGTCCGTCAACCAGGGCATCCTCTGCCTGAAGGGCCACTTCGGTTACGACTTCA
TCGACCGGCAGAAGCGGCTCACCACGCCGCTCATCCGAAAGAACGGGGAGCTGGTGGAGGCGTCGTGGGATGAGGCCATC
CACCTGGTCGCCTCCAAGATGAAGGAGACGATCCGGGACTACGGGCCTGACGCCCTGGCGGCCTTCAGCTCGGCCCGCTG
CACCAACGAAGAGAACTACCTGATGCAGAAGCTCATGCGCGCGGTGGTGGGCACCAACAACGTCGACCACTGCGCCCGTC
TCTGA

Upstream 100 bases:

>100_bases
TGGTTCGTGTACGTGCGCCAGGCCCCGGCGACCCCCGACCAGTCGATGGCGGCGTCGGCCGCGGACTAGGCGTGGATTGT
GAATCAAGGGAGGAGAACAC

Downstream 100 bases:

>100_bases
CACGCTCCGACGGTGTCCGGTCTGGGCACAGCGTTTGGTTCCGGAGCGATGACCAACACCATCAACGAACTGCAGGACAT
GGGGCCCGGCGATGCCATCT

Product: formate dehydrogenase-like protein

Products: CO2; NADH; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MKQVLTTCPYCGTGCTFYLNVEGDQVVGVTPNDDPRSVNQGILCLKGHFGYDFIDRQKRLTTPLIRKNGELVEASWDEAI
HLVASKMKETIRDYGPDALAAFSSARCTNEENYLMQKLMRAVVGTNNVDHCARL

Sequences:

>Translated_134_residues
MKQVLTTCPYCGTGCTFYLNVEGDQVVGVTPNDDPRSVNQGILCLKGHFGYDFIDRQKRLTTPLIRKNGELVEASWDEAI
HLVASKMKETIRDYGPDALAAFSSARCTNEENYLMQKLMRAVVGTNNVDHCARL
>Mature_134_residues
MKQVLTTCPYCGTGCTFYLNVEGDQVVGVTPNDDPRSVNQGILCLKGHFGYDFIDRQKRLTTPLIRKNGELVEASWDEAI
HLVASKMKETIRDYGPDALAAFSSARCTNEENYLMQKLMRAVVGTNNVDHCARL

Specific function: Decomposes Formic Acid To Hydrogen And Carbon Dioxide Under Anaerobic Conditions In The Absence Of Exogenous Electron Acceptors. [C]

COG id: COG3383

COG function: function code R; Uncharacterized anaerobic dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=141, Percent_Identity=47.5177304964539, Blast_Score=129, Evalue=7e-32,
Organism=Escherichia coli, GI1788534, Length=133, Percent_Identity=33.8345864661654, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009010
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006478
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR019574 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10588 NADH-G_4Fe-4S_3 [H]

EC number: 1.2.1.2 [C]

Molecular weight: Translated: 14960; Mature: 14960

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQVLTTCPYCGTGCTFYLNVEGDQVVGVTPNDDPRSVNQGILCLKGHFGYDFIDRQKRL
CCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
TTPLIRKNGELVEASWDEAIHLVASKMKETIRDYGPDALAAFSSARCTNEENYLMQKLMR
CCHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
AVVGTNNVDHCARL
HHHCCCCHHHHHCC
>Mature Secondary Structure
MKQVLTTCPYCGTGCTFYLNVEGDQVVGVTPNDDPRSVNQGILCLKGHFGYDFIDRQKRL
CCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEEEECCCCCCHHHHHHHH
TTPLIRKNGELVEASWDEAIHLVASKMKETIRDYGPDALAAFSSARCTNEENYLMQKLMR
CCHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
AVVGTNNVDHCARL
HHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: Fe; Mo; Se [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.032 {NAD+}} 0.54 {deamino-NAD+}} 0.19 {3-pyridinecarboxaldehyde-NAD+}} 1.16 {3-acetylpyridine-NAD+}} 0.17 {thio-NAD+}} [C]

Substrates: Formate; NAD(+) [C]

Specific reaction: Formate + NAD(+) = CO2 + NADH. formate = CO2 + H2 [C]

General reaction: Redox reaction [C]

Inhibitor: Azide; Br-; Cl-; CN-; F-; HCO3-; HCOS-; NO2-; NO3-; OCN-; SCN- [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579062; 9384377 [H]