Click here to switch to the map view.

The map label for this gene is folK [H]

Identifier: 51894321

GI number: 51894321

Start: 3394639

End: 3395166

Strand: Reverse

Name: folK [H]

Synonym: STH3186

Alternate gene names: 51894321

Gene position: 3395166-3394639 (Counterclockwise)

Preceding gene: 51894322

Following gene: 51894320

Centisome position: 95.21

GC content: 73.11

Gene sequence:

>528_bases
GTGACCACCGCGTACCTTTCGCTGGGGGCGAACATCGGCGACCCGGAGGGACAGCTGGTCTCGGCCCTGCGCCGGCTGGC
GTCCGCCCCAGGGACCAGCCTGGCCGCGGTGAGCGGGCTGTACCGCACGGCGCCCCAGGGGCTGACCGACCAGCCGGACT
TCCTGAACTGCGCCGCCCGCATCGAGACGGCCCTGGACCCGTACGGGTTGCTGGCCCTCACGCAGTCCATCGAGGCCGAC
CTGGGCCGGGTGCGCACGATCCGCTGGGGCCCGCGCACCATCGACATCGACATCCTGCTGCACGGGCAGGCGCGGCTGGA
CGGCCCCGGGCTCACCCTGCCCCACCCGCGCATGCTGGAGCGCGCCTTCGTGCTGGCGCCGCTGCTGGAGGTGTGGCGCG
ACGGGGAGGAGCCGGCCGGAATCAGCCGGACGGCGCTGGAGGGGCGGCTCTCGGACCTCGGGGACCAGCCGGTGGTGCGG
ATCCGGGACCGGCAGTCTTTGCTGAGGCAGATTCAGGGGGTAGAATAA

Upstream 100 bases:

>100_bases
GCAACGTGGAGGCCGTGACCATCCGGGTGCACAAGCCGCGGGCGCCGATCCCGGGCGCCCTGGGCGGCGTGATGGTGGAG
ATCCAGAGGAGGCGTGGCCG

Downstream 100 bases:

>100_bases
AGCCAATGGGCGGGACGGCCGGCACGGCCGGACAGTGAAGCCGAGGAGGTCTGCGGAATGACCCTGAACCCAGTTGCCGT
GGAGGAGTTGGTGCGGCGGG

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MTTAYLSLGANIGDPEGQLVSALRRLASAPGTSLAAVSGLYRTAPQGLTDQPDFLNCAARIETALDPYGLLALTQSIEAD
LGRVRTIRWGPRTIDIDILLHGQARLDGPGLTLPHPRMLERAFVLAPLLEVWRDGEEPAGISRTALEGRLSDLGDQPVVR
IRDRQSLLRQIQGVE

Sequences:

>Translated_175_residues
MTTAYLSLGANIGDPEGQLVSALRRLASAPGTSLAAVSGLYRTAPQGLTDQPDFLNCAARIETALDPYGLLALTQSIEAD
LGRVRTIRWGPRTIDIDILLHGQARLDGPGLTLPHPRMLERAFVLAPLLEVWRDGEEPAGISRTALEGRLSDLGDQPVVR
IRDRQSLLRQIQGVE
>Mature_174_residues
TTAYLSLGANIGDPEGQLVSALRRLASAPGTSLAAVSGLYRTAPQGLTDQPDFLNCAARIETALDPYGLLALTQSIEADL
GRVRTIRWGPRTIDIDILLHGQARLDGPGLTLPHPRMLERAFVLAPLLEVWRDGEEPAGISRTALEGRLSDLGDQPVVRI
RDRQSLLRQIQGVE

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=142, Percent_Identity=46.4788732394366, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324073, Length=134, Percent_Identity=39.5522388059701, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 18954; Mature: 18823

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00794 HPPK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAYLSLGANIGDPEGQLVSALRRLASAPGTSLAAVSGLYRTAPQGLTDQPDFLNCAAR
CCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IETALDPYGLLALTQSIEADLGRVRTIRWGPRTIDIDILLHGQARLDGPGLTLPHPRMLE
HHHHCCCHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEEEECCCEECCCCCCCCCHHHHH
RAFVLAPLLEVWRDGEEPAGISRTALEGRLSDLGDQPVVRIRDRQSLLRQIQGVE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCC
>Mature Secondary Structure 
TTAYLSLGANIGDPEGQLVSALRRLASAPGTSLAAVSGLYRTAPQGLTDQPDFLNCAAR
CEEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
IETALDPYGLLALTQSIEADLGRVRTIRWGPRTIDIDILLHGQARLDGPGLTLPHPRMLE
HHHHCCCHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEEEECCCEECCCCCCCCCHHHHH
RAFVLAPLLEVWRDGEEPAGISRTALEGRLSDLGDQPVVRIRDRQSLLRQIQGVE
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]