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The map label for this gene is dus1 [H]

Identifier: 51894293

GI number: 51894293

Start: 3354961

End: 3355998

Strand: Reverse

Name: dus1 [H]

Synonym: STH3158

Alternate gene names: 51894293

Gene position: 3355998-3354961 (Counterclockwise)

Preceding gene: 51894294

Following gene: 51894292

Centisome position: 94.11

GC content: 69.75

Gene sequence:

>1038_bases
GTGAAAGGGTTGTCCGAACCGCTCCTGAAGCCGATCCGCCTAGGCGGTCTGACCATCGAGCGTCCGCTGGCCCTGGCGCC
CATGGCCGGCGTCACCAACTGGCCCTTTCGCCGCCTCTGCAAGGAGCACGGCTGCGGCCTGGTGGTCACTGAGTTCGTCA
GCGACAAGGCGCTCCTGTACGACAGCAAGCGCACCCGGGAGATGATCCGGCTGCTGCCGGACGAGCGGCCCGCGGGGGTA
CAGCTCTTTGGCGCGGACCCCGACACGATGGCGCGGGCGGCGGCGCGCGTCGTGGAGCTGGAGCAGCCGGACCTGATCGA
CATCAACATGGGCTGCCCGGCGCCGAAGGTGACCAAGGGCCGGGGTGGCTCCTCCCTGCTGAAGGAGCCTGAACTGGCCC
AGGAGATCGTGCGCCGGGTGGTGCAGGCCGTCGCACCAACACCGGTGACGGTGAAGATGCGCATCGGCTGGGACTCCCGC
TCGATCAACGCCGTGGAGGTCGCGAAGCGGGTGGAGGAGGCCGGTGCGCGGATGATCACGGTGCATGGCCGCACCAAGGA
GCAGCATTACTCCGGCCGGGCCGACTGGGGCGTGATCGCCGCGGTCGCCCGGGCCGTATCGATCCCCGTGCTGGGCAACG
GCGACATCGCGGACCCCAGGGAGGCCGAGGCGCGGCTGCGCACGTCCGGGGTCGCAGGTCTCGCGGTGGGGCGGGGCGCG
CTCGGGAACCCCTGGATCTTCAGCCGCACCCTACATTACCTGGAGACGGGCGAGCTTCTGCCGGAGCCGGGGGTCCGGGA
GCGGGTGGAGACCGCGCTCCGCCACCTGGATCTGATGATTGAGTACAAGGGCGAGTTCCTGGCCGTCCGGGAGATGCGCA
AGCACGCCGCCTGGTATCTGAAAGGGCTCCACGGGTCCGCCGGGGTCCGGGCGGCGGTCAACGAGGCGGAGCGCGCAGAC
GACCTGCGCGCCCTCCTCCTGGGTTACCTGGACCGCATGGAGGCCGTCGCAGCGGAAAACGTCAGACTGTTAAGTTGA

Upstream 100 bases:

>100_bases
GGACGGCCGCGGTGATGCTGCCGCTGTTATGAGGGGTTAACATAGACCTCCCCGCGTGGCGTGGGTATAATAGACCCCGC
CACCGACTTTTTTTGCGGGG

Downstream 100 bases:

>100_bases
CACTGCGCAGCAGTGTCCGTATAATGAATTGACCACAGGCGTGGAGCACTGCGGGACGCACCGAATGGTCCGCCCCCGCA
GTGCTCCGTCGCCGCACCGG

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MKGLSEPLLKPIRLGGLTIERPLALAPMAGVTNWPFRRLCKEHGCGLVVTEFVSDKALLYDSKRTREMIRLLPDERPAGV
QLFGADPDTMARAAARVVELEQPDLIDINMGCPAPKVTKGRGGSSLLKEPELAQEIVRRVVQAVAPTPVTVKMRIGWDSR
SINAVEVAKRVEEAGARMITVHGRTKEQHYSGRADWGVIAAVARAVSIPVLGNGDIADPREAEARLRTSGVAGLAVGRGA
LGNPWIFSRTLHYLETGELLPEPGVRERVETALRHLDLMIEYKGEFLAVREMRKHAAWYLKGLHGSAGVRAAVNEAERAD
DLRALLLGYLDRMEAVAAENVRLLS

Sequences:

>Translated_345_residues
MKGLSEPLLKPIRLGGLTIERPLALAPMAGVTNWPFRRLCKEHGCGLVVTEFVSDKALLYDSKRTREMIRLLPDERPAGV
QLFGADPDTMARAAARVVELEQPDLIDINMGCPAPKVTKGRGGSSLLKEPELAQEIVRRVVQAVAPTPVTVKMRIGWDSR
SINAVEVAKRVEEAGARMITVHGRTKEQHYSGRADWGVIAAVARAVSIPVLGNGDIADPREAEARLRTSGVAGLAVGRGA
LGNPWIFSRTLHYLETGELLPEPGVRERVETALRHLDLMIEYKGEFLAVREMRKHAAWYLKGLHGSAGVRAAVNEAERAD
DLRALLLGYLDRMEAVAAENVRLLS
>Mature_345_residues
MKGLSEPLLKPIRLGGLTIERPLALAPMAGVTNWPFRRLCKEHGCGLVVTEFVSDKALLYDSKRTREMIRLLPDERPAGV
QLFGADPDTMARAAARVVELEQPDLIDINMGCPAPKVTKGRGGSSLLKEPELAQEIVRRVVQAVAPTPVTVKMRIGWDSR
SINAVEVAKRVEEAGARMITVHGRTKEQHYSGRADWGVIAAVARAVSIPVLGNGDIADPREAEARLRTSGVAGLAVGRGA
LGNPWIFSRTLHYLETGELLPEPGVRERVETALRHLDLMIEYKGEFLAVREMRKHAAWYLKGLHGSAGVRAAVNEAERAD
DLRALLLGYLDRMEAVAAENVRLLS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=240, Percent_Identity=37.0833333333333, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=36.9294605809129, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI40807366, Length=227, Percent_Identity=34.3612334801762, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=33.0396475770925, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI8923374, Length=256, Percent_Identity=31.640625, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789660, Length=324, Percent_Identity=39.5061728395062, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788462, Length=247, Percent_Identity=39.2712550607287, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=28.4552845528455, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543114, Length=244, Percent_Identity=35.2459016393443, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17507177, Length=268, Percent_Identity=32.8358208955224, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144369, Length=228, Percent_Identity=29.8245614035088, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=285, Percent_Identity=25.9649122807018, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=239, Percent_Identity=31.7991631799163, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=309, Percent_Identity=24.5954692556634, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323437, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24585320, Length=227, Percent_Identity=37.0044052863436, Blast_Score=146, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24580595, Length=250, Percent_Identity=33.6, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19920448, Length=250, Percent_Identity=33.6, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921524, Length=226, Percent_Identity=28.7610619469027, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45549423, Length=216, Percent_Identity=31.0185185185185, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37747; Mature: 37747

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLSEPLLKPIRLGGLTIERPLALAPMAGVTNWPFRRLCKEHGCGLVVTEFVSDKALLY
CCCCHHHHHHHHHHCCEEECCCCHHCCCCCCCCCHHHHHHHHCCCCEEEEHHHCCCCEEE
DSKRTREMIRLLPDERPAGVQLFGADPDTMARAAARVVELEQPDLIDINMGCPAPKVTKG
CCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
RGGSSLLKEPELAQEIVRRVVQAVAPTPVTVKMRIGWDSRSINAVEVAKRVEEAGARMIT
CCCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEE
VHGRTKEQHYSGRADWGVIAAVARAVSIPVLGNGDIADPREAEARLRTSGVAGLAVGRGA
EECCCCHHHCCCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCCEEECCCC
LGNPWIFSRTLHYLETGELLPEPGVRERVETALRHLDLMIEYKGEFLAVREMRKHAAWYL
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
KGLHGSAGVRAAVNEAERADDLRALLLGYLDRMEAVAAENVRLLS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MKGLSEPLLKPIRLGGLTIERPLALAPMAGVTNWPFRRLCKEHGCGLVVTEFVSDKALLY
CCCCHHHHHHHHHHCCEEECCCCHHCCCCCCCCCHHHHHHHHCCCCEEEEHHHCCCCEEE
DSKRTREMIRLLPDERPAGVQLFGADPDTMARAAARVVELEQPDLIDINMGCPAPKVTKG
CCHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCC
RGGSSLLKEPELAQEIVRRVVQAVAPTPVTVKMRIGWDSRSINAVEVAKRVEEAGARMIT
CCCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEE
VHGRTKEQHYSGRADWGVIAAVARAVSIPVLGNGDIADPREAEARLRTSGVAGLAVGRGA
EECCCCHHHCCCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCCEEECCCC
LGNPWIFSRTLHYLETGELLPEPGVRERVETALRHLDLMIEYKGEFLAVREMRKHAAWYL
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
KGLHGSAGVRAAVNEAERADDLRALLLGYLDRMEAVAAENVRLLS
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]