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The map label for this gene is greA2 [H]

Identifier: 51894292

GI number: 51894292

Start: 3354272

End: 3354745

Strand: Reverse

Name: greA2 [H]

Synonym: STH3157

Alternate gene names: 51894292

Gene position: 3354745-3354272 (Counterclockwise)

Preceding gene: 51894293

Following gene: 51894289

Centisome position: 94.07

GC content: 63.5

Gene sequence:

>474_bases
ATGGCCGAGAAAGAGGTTCTGCTATCCCTGGACGGCTTGAAGCAGCTCGAGCAGGAGCTGCTGTACCTCCGCACCGTGAA
GCGCATGGAGGTCGCTGAGCGCATCAAGCAGGCCCGCGAGTACGGGGACATCTCCGAGAACTCCGAATACGAGGATGCGA
AGAACGAGCAGGCCTTCATCGAAGGGCGCATTCTGGTGCTGGAGAAGATGCTGCGCAACGCGCGGGTGATTGATGAGCCC
GTCGGCCAGCAGGAGGTGGTGGTGCTCGGCAGCACGGTGGTGCTGAGGGACCTGGAGTATGGCGACGAGCATACGTACAC
CATCGTCGGCACCACGGAGGCCGATCCCGCCCACAACCGGATCTCCAACGAGTCCCCGGTCGGCCGCGCCATCATGGGCC
AGCGGGTGGGCACCGTGGTGGCCGTCGATGCGCCGGACGGGGTCTTTAAGTACGAGATCGTCGCGATCAAGTGA

Upstream 100 bases:

>100_bases
TATACTGCGGCCAGCCCGGGTCGTATACATCCCTACCACAACGAGCGTACCACCGCCACGGCACCGTGGTGTGGCTGACT
GGTGTAAAGGGGAGAACCAC

Downstream 100 bases:

>100_bases
GCAGGCGGGGGGCATGGGTGCCCCCCGCTCGGTGTGCAGGCCTGCAGTCAGTCTTTGACGTCCGCGCCCAGCCGCAGCGC
CGGGCCGCTGGGGCGCCGCT

Product: transcription elongation factor

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAEKEVLLSLDGLKQLEQELLYLRTVKRMEVAERIKQAREYGDISENSEYEDAKNEQAFIEGRILVLEKMLRNARVIDEP
VGQQEVVVLGSTVVLRDLEYGDEHTYTIVGTTEADPAHNRISNESPVGRAIMGQRVGTVVAVDAPDGVFKYEIVAIK

Sequences:

>Translated_157_residues
MAEKEVLLSLDGLKQLEQELLYLRTVKRMEVAERIKQAREYGDISENSEYEDAKNEQAFIEGRILVLEKMLRNARVIDEP
VGQQEVVVLGSTVVLRDLEYGDEHTYTIVGTTEADPAHNRISNESPVGRAIMGQRVGTVVAVDAPDGVFKYEIVAIK
>Mature_156_residues
AEKEVLLSLDGLKQLEQELLYLRTVKRMEVAERIKQAREYGDISENSEYEDAKNEQAFIEGRILVLEKMLRNARVIDEPV
GQQEVVVLGSTVVLRDLEYGDEHTYTIVGTTEADPAHNRISNESPVGRAIMGQRVGTVVAVDAPDGVFKYEIVAIK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=38.562091503268, Blast_Score=112, Evalue=9e-27,
Organism=Escherichia coli, GI87082261, Length=153, Percent_Identity=32.6797385620915, Blast_Score=78, Evalue=2e-16,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17618; Mature: 17487

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKEVLLSLDGLKQLEQELLYLRTVKRMEVAERIKQAREYGDISENSEYEDAKNEQAFI
CCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
EGRILVLEKMLRNARVIDEPVGQQEVVVLGSTVVLRDLEYGDEHTYTIVGTTEADPAHNR
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCEEEEEEECCCCCCCHHC
ISNESPVGRAIMGQRVGTVVAVDAPDGVFKYEIVAIK
CCCCCCCCHHHHHCCCCEEEEEECCCCEEEEEEEEEC
>Mature Secondary Structure 
AEKEVLLSLDGLKQLEQELLYLRTVKRMEVAERIKQAREYGDISENSEYEDAKNEQAFI
CCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
EGRILVLEKMLRNARVIDEPVGQQEVVVLGSTVVLRDLEYGDEHTYTIVGTTEADPAHNR
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCEEEEEEECCCCCCCHHC
ISNESPVGRAIMGQRVGTVVAVDAPDGVFKYEIVAIK
CCCCCCCCHHHHHCCCCEEEEEECCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA