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The map label for this gene is purF [H]

Identifier: 51893992

GI number: 51893992

Start: 3075729

End: 3077216

Strand: Reverse

Name: purF [H]

Synonym: STH2854

Alternate gene names: 51893992

Gene position: 3077216-3075729 (Counterclockwise)

Preceding gene: 51893993

Following gene: 51893991

Centisome position: 86.29

GC content: 71.24

Gene sequence:

>1488_bases
GTGATCCGGCCCGGTGACCCGACCCTGGCCCTGGCCCTGCAGCCCTACCTGGGCGGGTGGCCGACCAACCCGGACAAGGG
CCCGGCGGACGAGTGCGGCGTCTTCGGCATCTACGGCCACCCTGAGGCGGCGCGGGTGGTGTACCACGCCCTGATCGCCC
TGCAGCACCGGGGGCAGGAGTCCGCGGGCATCGTGGCCGCGGACGGTGCGAACCTGAACACCCACCGGGGCATGGGGCTG
GTCAGCGACGTGTTCGAGAAGCCCGAGACCATCCAGCGGCTGGCCGGCGACATCGCCATCGGCCACGTGCGGTATTCGAC
CACCGGCTCCTCCCGGCTGGGCAACGCCCAGCCCGTGGTGGTGAACACCCGGCGCGGCGGACTGGCCCTGGCGCACAACG
GCAACCTCGTGGACGCCCCGGCCATCCGGGACCGGCTGGAGGAGCAGGGGGCGATCTTCACCACGTCCATCGACACCGAG
GTGCTGGCCCACCTGATCGTCCGTTCCCGGGCGAAGAGCCTGGAGGACGCGATCGTCGACGCCGTGAGCCAGGTGCACGG
CGGCTATGCCCTGCTCATCCTGGCCGAGGACCGGCTGATCGGCATCCGGGATCCCCACGGCATCCGCCCGCTGCAGCTGG
GCCGGCTTGACGGCAGCTGGGTGCTGGCCTCCGAGACCTGCGCCTTTGACACCATCGGCGCGGAGTTCGTGCGGGAGGTG
GCCCCCGGCGAGATGGTGACCATCAGCGAGGGGGGCAAGCTCCGGTCCCGGGCGGCGGTCAGGGAGGCCGTCGCCCCCCG
GCCGTGCATCTTCGAGTTCATCTACTTCGCCCGTCCCGACTCGCAGTTCGTCGGGGTCAACGTGCACACCGTGCGCAAGG
CGATGGGCCGCCAGCTCGCGAAGGAGGCGCCGGCGGATGCCGACATCGTCATCGGGGTGCCCGACTCCTCGATCTCCGCG
GCCACCGGCTACGCGGAGGAGTCCGGCATCCCCTACGAGGTCGGGCTGGTCAAGAACCGCTACATCGCCCGGACGTTCAT
CCTGCCGTCCCAGGCCGGGCGCGAGTCGGCGCTGAAGCTGAAGCTGAACCCGCTGCGCAAGGTGATCGAGGGCAGGCGGG
TGGTGCTGGTGGACGACTCCATCGTGCGCGGCACCACGTCCCGCCACCTGGTGAGCCTCCTCCGGGAGGCCGGGGCGCGG
GAGGTGCACCTGCGCATCGCATCGCCGCCGTACCAGAACGCCTGCCACTACGGCATCGATACCTCCAAGTCCACCGACCT
GATCGCCCGCGGGCGCACGGTCCGGGAGATCGCCGACGCCATCGGCGCCGACTCCCTGGCCTACCTCTCGGTGGAGGGCA
TGGTGAAGGCCACCGGCCTGTCGCCGGAAGCGGGCTTCTGCCTGGCCTGCTTCACCGGCGACTACCCGGTGCCCGTGCCG
GAAGAGGCCGACAAGTACGCGCTGGAGGGGGGCTGCGGCTGTGACTGA

Upstream 100 bases:

>100_bases
GCGGGTCGGCCCACGTCTACCGGCGTCAGGAGCTGATCGACCTGCCGGTCGCGGACCTGGTGCGTGCGCACAAGGAGGCG
ATCCCCAGGTGGATGGACGC

Downstream 100 bases:

>100_bases
GAAGGGGCTCACCTACGCCGACGCCGGCGTCAACCGGGAGCGGCACTACGAACTGGTGCGGCGGATCGCCGCCCACACGG
CCCGCACCCTCCGGCGGCCC

Product: phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MIRPGDPTLALALQPYLGGWPTNPDKGPADECGVFGIYGHPEAARVVYHALIALQHRGQESAGIVAADGANLNTHRGMGL
VSDVFEKPETIQRLAGDIAIGHVRYSTTGSSRLGNAQPVVVNTRRGGLALAHNGNLVDAPAIRDRLEEQGAIFTTSIDTE
VLAHLIVRSRAKSLEDAIVDAVSQVHGGYALLILAEDRLIGIRDPHGIRPLQLGRLDGSWVLASETCAFDTIGAEFVREV
APGEMVTISEGGKLRSRAAVREAVAPRPCIFEFIYFARPDSQFVGVNVHTVRKAMGRQLAKEAPADADIVIGVPDSSISA
ATGYAEESGIPYEVGLVKNRYIARTFILPSQAGRESALKLKLNPLRKVIEGRRVVLVDDSIVRGTTSRHLVSLLREAGAR
EVHLRIASPPYQNACHYGIDTSKSTDLIARGRTVREIADAIGADSLAYLSVEGMVKATGLSPEAGFCLACFTGDYPVPVP
EEADKYALEGGCGCD

Sequences:

>Translated_495_residues
MIRPGDPTLALALQPYLGGWPTNPDKGPADECGVFGIYGHPEAARVVYHALIALQHRGQESAGIVAADGANLNTHRGMGL
VSDVFEKPETIQRLAGDIAIGHVRYSTTGSSRLGNAQPVVVNTRRGGLALAHNGNLVDAPAIRDRLEEQGAIFTTSIDTE
VLAHLIVRSRAKSLEDAIVDAVSQVHGGYALLILAEDRLIGIRDPHGIRPLQLGRLDGSWVLASETCAFDTIGAEFVREV
APGEMVTISEGGKLRSRAAVREAVAPRPCIFEFIYFARPDSQFVGVNVHTVRKAMGRQLAKEAPADADIVIGVPDSSISA
ATGYAEESGIPYEVGLVKNRYIARTFILPSQAGRESALKLKLNPLRKVIEGRRVVLVDDSIVRGTTSRHLVSLLREAGAR
EVHLRIASPPYQNACHYGIDTSKSTDLIARGRTVREIADAIGADSLAYLSVEGMVKATGLSPEAGFCLACFTGDYPVPVP
EEADKYALEGGCGCD
>Mature_495_residues
MIRPGDPTLALALQPYLGGWPTNPDKGPADECGVFGIYGHPEAARVVYHALIALQHRGQESAGIVAADGANLNTHRGMGL
VSDVFEKPETIQRLAGDIAIGHVRYSTTGSSRLGNAQPVVVNTRRGGLALAHNGNLVDAPAIRDRLEEQGAIFTTSIDTE
VLAHLIVRSRAKSLEDAIVDAVSQVHGGYALLILAEDRLIGIRDPHGIRPLQLGRLDGSWVLASETCAFDTIGAEFVREV
APGEMVTISEGGKLRSRAAVREAVAPRPCIFEFIYFARPDSQFVGVNVHTVRKAMGRQLAKEAPADADIVIGVPDSSISA
ATGYAEESGIPYEVGLVKNRYIARTFILPSQAGRESALKLKLNPLRKVIEGRRVVLVDDSIVRGTTSRHLVSLLREAGAR
EVHLRIASPPYQNACHYGIDTSKSTDLIARGRTVREIADAIGADSLAYLSVEGMVKATGLSPEAGFCLACFTGDYPVPVP
EEADKYALEGGCGCD

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=507, Percent_Identity=40.6311637080868, Blast_Score=357, Evalue=2e-98,
Organism=Escherichia coli, GI1788651, Length=468, Percent_Identity=41.2393162393162, Blast_Score=318, Evalue=6e-88,
Organism=Escherichia coli, GI1790167, Length=212, Percent_Identity=27.8301886792453, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=43.5684647302905, Blast_Score=348, Evalue=4e-96,
Organism=Saccharomyces cerevisiae, GI6323958, Length=472, Percent_Identity=37.2881355932203, Blast_Score=276, Evalue=6e-75,
Organism=Drosophila melanogaster, GI24659598, Length=494, Percent_Identity=43.7246963562753, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=44.8275862068966, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI28573187, Length=507, Percent_Identity=43.1952662721894, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52877; Mature: 52877

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPGDPTLALALQPYLGGWPTNPDKGPADECGVFGIYGHPEAARVVYHALIALQHRGQE
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCC
SAGIVAADGANLNTHRGMGLVSDVFEKPETIQRLAGDIAIGHVRYSTTGSSRLGNAQPVV
CCCEEEECCCCCCCCCCCCHHHHHHCCHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCEE
VNTRRGGLALAHNGNLVDAPAIRDRLEEQGAIFTTSIDTEVLAHLIVRSRAKSLEDAIVD
EEECCCCEEEEECCCEECCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AVSQVHGGYALLILAEDRLIGIRDPHGIRPLQLGRLDGSWVLASETCAFDTIGAEFVREV
HHHHHCCCEEEEEEECCCEEECCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHC
APGEMVTISEGGKLRSRAAVREAVAPRPCIFEFIYFARPDSQFVGVNVHTVRKAMGRQLA
CCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHH
KEAPADADIVIGVPDSSISAATGYAEESGIPYEVGLVKNRYIARTFILPSQAGRESALKL
HHCCCCCEEEEECCCCCCCHHCCCHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEE
KLNPLRKVIEGRRVVLVDDSIVRGTTSRHLVSLLREAGAREVHLRIASPPYQNACHYGID
EEHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
TSKSTDLIARGRTVREIADAIGADSLAYLSVEGMVKATGLSPEAGFCLACFTGDYPVPVP
CCCCCHHHHCCCHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCEEEEEECCCCCCCCC
EEADKYALEGGCGCD
CCCCCEEECCCCCCC
>Mature Secondary Structure
MIRPGDPTLALALQPYLGGWPTNPDKGPADECGVFGIYGHPEAARVVYHALIALQHRGQE
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCC
SAGIVAADGANLNTHRGMGLVSDVFEKPETIQRLAGDIAIGHVRYSTTGSSRLGNAQPVV
CCCEEEECCCCCCCCCCCCHHHHHHCCHHHHHHHHCCEEEEEEEEECCCCCCCCCCCCEE
VNTRRGGLALAHNGNLVDAPAIRDRLEEQGAIFTTSIDTEVLAHLIVRSRAKSLEDAIVD
EEECCCCEEEEECCCEECCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AVSQVHGGYALLILAEDRLIGIRDPHGIRPLQLGRLDGSWVLASETCAFDTIGAEFVREV
HHHHHCCCEEEEEEECCCEEECCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHHHHHHC
APGEMVTISEGGKLRSRAAVREAVAPRPCIFEFIYFARPDSQFVGVNVHTVRKAMGRQLA
CCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECHHHHHHHHHHHHHH
KEAPADADIVIGVPDSSISAATGYAEESGIPYEVGLVKNRYIARTFILPSQAGRESALKL
HHCCCCCEEEEECCCCCCCHHCCCHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCEEEE
KLNPLRKVIEGRRVVLVDDSIVRGTTSRHLVSLLREAGAREVHLRIASPPYQNACHYGID
EEHHHHHHHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCC
TSKSTDLIARGRTVREIADAIGADSLAYLSVEGMVKATGLSPEAGFCLACFTGDYPVPVP
CCCCCHHHHCCCHHHHHHHHHCCCCEEEEEECCEEEECCCCCCCCEEEEEECCCCCCCCC
EEADKYALEGGCGCD
CCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]