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The map label for this gene is hisS2

Identifier: 51893977

GI number: 51893977

Start: 3059322

End: 3060521

Strand: Reverse

Name: hisS2

Synonym: STH2839

Alternate gene names: 51893977

Gene position: 3060521-3059322 (Counterclockwise)

Preceding gene: 51893978

Following gene: 51893976

Centisome position: 85.82

GC content: 73.58

Gene sequence:

>1200_bases
GTGAGCACACCGGTGACCCGGACGCAGGTGCCCGACGGAGTGCGCGACCGGCTGCCGTCGGAAGCGGCAGCAATGCGCCG
CCTCTCCGCAAGGCTGGACAACGTCTTCCGCGCCTGGGGTTACCGGGAGGTCTCCACTCCGGTCATCGAGTACCTGGAGG
CGGTTGCCGCCGGCGCGGCCAACTGGGGCCGCCGGGAGGACCTGTACCAGTTCTTCGACCGAAAGGGGCGCACCCTGGCC
TTGCGCCCGGACATGACAACCCCCATCGCCCGCCTGATGGCGACCCGGCTGGCCGACGAGCCCCTGCCGCTCCGGCTTTC
TTATTTTGCGCCGGTCTTCCGGCACCGGGAACTCAGGGCCGGCGCGACCAGTGAGATCTGGCAGGCCGGGGTGGAGCTGG
TCGGGGCGCCGGGGGAGGCCGCCGACGCGGAGGTCATCAGCCTGGCGTGCGCCGCGGTCCGGGCGGCGGACCTCACCGGT
TTTCGTATCGGTCTGGGCCACGTCGGCGTCGTGGAGGGGCTGTTTGAGAGCGCCGGCGTCGATCCTCAGGCCGCGGCCGT
GCTGAAGGAGGCGATGGTCGCCCGGGATCTTGTGGCCTTCGAGCAGGGGGTCGCCCGGGCGGGCCTCTCCGGCGAGCGGG
CGGAACGGCTGCTGGCGCTGGTCCACTTCCACGGCAGTTATGCGGAGGCCGTGGCCCGCTTCGGCGGCGTCGGTGGCCGG
GTGGCGGAGGCGCTGCAGCACCTGGGCCGGGTGCTCGAGGTGCTGGAGGCCCTGGGGGTGGCCGAGCAGGTCAACCTCGA
CCTGGGGCTGGTGCGGTCCCTGGGCTACTACACCGGGGTCGTCTTCGAGGGCTATCTCCCGGGCATCGGGGCGCCGGTCC
TGGGCGGCGGCCGGTACGACAACCTGGTCGCGGAGTTCGGCCGGCCGCTGGCAGCGACGGGCTTCGCCCTGGAGGTGGAC
CGGCTGCTGATGGCGCAGGAGCAGCAGGGTGCTCTCGCCGCGGAGCCGGGCCTTGACGCCGTGATCGCCTGCCCGCCCGG
GCAGGAGGCGGCGGCCATGGCCTGGGCGGCGCGGCTCCGGGCGCAGGGGCTGGCCGTGGAGGTGGATTTCCTGGACCGGA
CCGGGGAGGAGCTGGCCGCGTACGCTCGCGCCCGGGCCGCGGCACGGGTGTTGCGCCCGGGGGTGCCGTCGATTCACTAG

Upstream 100 bases:

>100_bases
TCGGGTTTTTCGCGCCCTCTTGACCAAAGCGGTCGATTATGGTAGCCTCGTTTTAGCCATTTATCACATTAGCCTACTAA
AGTACAGGAGGGCGATGGCC

Downstream 100 bases:

>100_bases
CCGTTGCAGGCGCCGGGCAGCGGGGCTCGGCGCCGCAGGCCCCGGGGCCCCGGCACCCCGGCACCCATGAAGTCTTCCAG
GAGGGAACTGCATTCATGTC

Product: histidyl-tRNA synthetase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSTPVTRTQVPDGVRDRLPSEAAAMRRLSARLDNVFRAWGYREVSTPVIEYLEAVAAGAANWGRREDLYQFFDRKGRTLA
LRPDMTTPIARLMATRLADEPLPLRLSYFAPVFRHRELRAGATSEIWQAGVELVGAPGEAADAEVISLACAAVRAADLTG
FRIGLGHVGVVEGLFESAGVDPQAAAVLKEAMVARDLVAFEQGVARAGLSGERAERLLALVHFHGSYAEAVARFGGVGGR
VAEALQHLGRVLEVLEALGVAEQVNLDLGLVRSLGYYTGVVFEGYLPGIGAPVLGGGRYDNLVAEFGRPLAATGFALEVD
RLLMAQEQQGALAAEPGLDAVIACPPGQEAAAMAWAARLRAQGLAVEVDFLDRTGEELAAYARARAAARVLRPGVPSIH

Sequences:

>Translated_399_residues
MSTPVTRTQVPDGVRDRLPSEAAAMRRLSARLDNVFRAWGYREVSTPVIEYLEAVAAGAANWGRREDLYQFFDRKGRTLA
LRPDMTTPIARLMATRLADEPLPLRLSYFAPVFRHRELRAGATSEIWQAGVELVGAPGEAADAEVISLACAAVRAADLTG
FRIGLGHVGVVEGLFESAGVDPQAAAVLKEAMVARDLVAFEQGVARAGLSGERAERLLALVHFHGSYAEAVARFGGVGGR
VAEALQHLGRVLEVLEALGVAEQVNLDLGLVRSLGYYTGVVFEGYLPGIGAPVLGGGRYDNLVAEFGRPLAATGFALEVD
RLLMAQEQQGALAAEPGLDAVIACPPGQEAAAMAWAARLRAQGLAVEVDFLDRTGEELAAYARARAAARVLRPGVPSIH
>Mature_398_residues
STPVTRTQVPDGVRDRLPSEAAAMRRLSARLDNVFRAWGYREVSTPVIEYLEAVAAGAANWGRREDLYQFFDRKGRTLAL
RPDMTTPIARLMATRLADEPLPLRLSYFAPVFRHRELRAGATSEIWQAGVELVGAPGEAADAEVISLACAAVRAADLTGF
RIGLGHVGVVEGLFESAGVDPQAAAVLKEAMVARDLVAFEQGVARAGLSGERAERLLALVHFHGSYAEAVARFGGVGGRV
AEALQHLGRVLEVLEALGVAEQVNLDLGLVRSLGYYTGVVFEGYLPGIGAPVLGGGRYDNLVAEFGRPLAATGFALEVDR
LLMAQEQQGALAAEPGLDAVIACPPGQEAAAMAWAARLRAQGLAVEVDFLDRTGEELAAYARARAAARVLRPGVPSIH

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI6996014, Length=347, Percent_Identity=26.8011527377522, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI15029520, Length=352, Percent_Identity=25.8522727272727, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788861, Length=366, Percent_Identity=30.8743169398907, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71993686, Length=346, Percent_Identity=23.121387283237, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71993693, Length=359, Percent_Identity=22.8412256267409, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6325290, Length=375, Percent_Identity=23.2, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24643061, Length=363, Percent_Identity=25.8953168044077, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24643059, Length=381, Percent_Identity=25.1968503937008, Blast_Score=102, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_SYMTH (Q67KH4)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076668.1
- ProteinModelPortal:   Q67KH4
- SMR:   Q67KH4
- GeneID:   2979791
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2839
- NMPDR:   fig|292459.1.peg.2714
- HOGENOM:   HBG616575
- OMA:   YIDLEIL
- BioCyc:   STHE292459:STH2839-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 42441; Mature: 42310

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPVTRTQVPDGVRDRLPSEAAAMRRLSARLDNVFRAWGYREVSTPVIEYLEAVAAGAA
CCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHCCC
NWGRREDLYQFFDRKGRTLALRPDMTTPIARLMATRLADEPLPLRLSYFAPVFRHRELRA
CCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCC
GATSEIWQAGVELVGAPGEAADAEVISLACAAVRAADLTGFRIGLGHVGVVEGLFESAGV
CCHHHHHHHCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCC
DPQAAAVLKEAMVARDLVAFEQGVARAGLSGERAERLLALVHFHGSYAEAVARFGGVGGR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
VAEALQHLGRVLEVLEALGVAEQVNLDLGLVRSLGYYTGVVFEGYLPGIGAPVLGGGRYD
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHH
NLVAEFGRPLAATGFALEVDRLLMAQEQQGALAAEPGLDAVIACPPGQEAAAMAWAARLR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCCHHHHHHHHHHHH
AQGLAVEVDFLDRTGEELAAYARARAAARVLRPGVPSIH
HCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STPVTRTQVPDGVRDRLPSEAAAMRRLSARLDNVFRAWGYREVSTPVIEYLEAVAAGAA
CCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHCCC
NWGRREDLYQFFDRKGRTLALRPDMTTPIARLMATRLADEPLPLRLSYFAPVFRHRELRA
CCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHCC
GATSEIWQAGVELVGAPGEAADAEVISLACAAVRAADLTGFRIGLGHVGVVEGLFESAGV
CCHHHHHHHCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCC
DPQAAAVLKEAMVARDLVAFEQGVARAGLSGERAERLLALVHFHGSYAEAVARFGGVGGR
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHH
VAEALQHLGRVLEVLEALGVAEQVNLDLGLVRSLGYYTGVVFEGYLPGIGAPVLGGGRYD
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHH
NLVAEFGRPLAATGFALEVDRLLMAQEQQGALAAEPGLDAVIACPPGQEAAAMAWAARLR
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCEEEECCCCCHHHHHHHHHHHH
AQGLAVEVDFLDRTGEELAAYARARAAARVLRPGVPSIH
HCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA