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The map label for this gene is hisH

Identifier: 51893973

GI number: 51893973

Start: 3055948

End: 3056586

Strand: Reverse

Name: hisH

Synonym: STH2835

Alternate gene names: 51893973

Gene position: 3056586-3055948 (Counterclockwise)

Preceding gene: 51893974

Following gene: 51893972

Centisome position: 85.71

GC content: 73.24

Gene sequence:

>639_bases
ATGGCCCGGATCGTCATCGTGGACTACGGGATGGGCAACCTCGCCAGCGTCCGGAACGCCCTGCGGGCGGTGGGCTTCGA
GGCGGCGGTGAGCGACGACCCGGCGGCGGTGGCGGGGGCGGACGGCCTGGTGCTGCCCGGCGTGGGCGCGTTCGGCACCG
GCATGCAGAACCTGGCCCGCCGCGGCCTCGATCAGGCCGTGCGGCAGGCGGCCGCAGCCGGCCGGCCGGTCCTGGGCATC
TGCCTGGGGATGCAGCTCCTCCTCGCGGAGGGCGACGAGGGCGGCCCGCGCCCGGGGCTGGGCCTCCTGGAGGGGCGGGT
TGCGCGCCTTCCCGACGGGCTGCCCCTTCCCCAGATCGGCTGGAACCTGGTGGAGCCGCAGCGGGACCACCCGCTCTTCG
CCGGGCTGCCGACCCCCTTCTGGGCCTATTTCGACCACGCTTACGCGGTGGAGGGCGAACCGCCGTCCACGGCGCTGGCC
CTGACCGACTACGGCCGCACCTACCCCTCGGTGGTGGGCCGCGGCAACCTGCTGGGTATCCAGTTCCATCCCGAGAAGTC
CTCCCGGGCGGGACTCCGGATGCTGGCCAACTGGGGGAGAATGGTATGCGACTTGATCTCTACCCGGCCATCGACCTGA

Upstream 100 bases:

>100_bases
TCGAGGGTCTGTTCAAGGCTTTCGCCCGGGCCCTCGGCGATGCCGTGGCGCGGGATCCCCGGGTCCTGGGGGTCCCCTCC
ACCAAGGGGGCCCTGTTCTG

Downstream 100 bases:

>100_bases
AGGACGGCCAGGTGGTGCGGCTCCGGCAGGGGCGCATGGACGAGGCCACGGTCTATGGGGTGGACCCGGTCCGGATCGCG
GCTCGCTGGGCGGAGGCCGG

Product: imidazole glycerol phosphate synthase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MARIVIVDYGMGNLASVRNALRAVGFEAAVSDDPAAVAGADGLVLPGVGAFGTGMQNLARRGLDQAVRQAAAAGRPVLGI
CLGMQLLLAEGDEGGPRPGLGLLEGRVARLPDGLPLPQIGWNLVEPQRDHPLFAGLPTPFWAYFDHAYAVEGEPPSTALA
LTDYGRTYPSVVGRGNLLGIQFHPEKSSRAGLRMLANWGRMVCDLISTRPST

Sequences:

>Translated_212_residues
MARIVIVDYGMGNLASVRNALRAVGFEAAVSDDPAAVAGADGLVLPGVGAFGTGMQNLARRGLDQAVRQAAAAGRPVLGI
CLGMQLLLAEGDEGGPRPGLGLLEGRVARLPDGLPLPQIGWNLVEPQRDHPLFAGLPTPFWAYFDHAYAVEGEPPSTALA
LTDYGRTYPSVVGRGNLLGIQFHPEKSSRAGLRMLANWGRMVCDLISTRPST
>Mature_211_residues
ARIVIVDYGMGNLASVRNALRAVGFEAAVSDDPAAVAGADGLVLPGVGAFGTGMQNLARRGLDQAVRQAAAAGRPVLGIC
LGMQLLLAEGDEGGPRPGLGLLEGRVARLPDGLPLPQIGWNLVEPQRDHPLFAGLPTPFWAYFDHAYAVEGEPPSTALAL
TDYGRTYPSVVGRGNLLGIQFHPEKSSRAGLRMLANWGRMVCDLISTRPST

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=41, Blast_Score=143, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6319725, Length=210, Percent_Identity=33.3333333333333, Blast_Score=128, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_SYMTH (Q67KH8)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076664.1
- ProteinModelPortal:   Q67KH8
- SMR:   Q67KH8
- GeneID:   2979862
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2835
- NMPDR:   fig|292459.1.peg.2710
- HOGENOM:   HBG292341
- OMA:   ASENFED
- BioCyc:   STHE292459:STH2835-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22294; Mature: 22163

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 183-183 ACT_SITE 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIVIVDYGMGNLASVRNALRAVGFEAAVSDDPAAVAGADGLVLPGVGAFGTGMQNLAR
CEEEEEEECCCCCHHHHHHHHHHHCCHHHCCCCCCEEECCCCEEECCCCCHHHHHHHHHH
RGLDQAVRQAAAAGRPVLGICLGMQLLLAEGDEGGPRPGLGLLEGRVARLPDGLPLPQIG
HHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHCHHHHCCCCCCCCCCC
WNLVEPQRDHPLFAGLPTPFWAYFDHAYAVEGEPPSTALALTDYGRTYPSVVGRGNLLGI
CCCCCCCCCCCEEECCCCHHHHHHCCEEEECCCCCCCEEEECCCCCHHHHHHCCCCEEEE
QFHPEKSSRAGLRMLANWGRMVCDLISTRPST
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ARIVIVDYGMGNLASVRNALRAVGFEAAVSDDPAAVAGADGLVLPGVGAFGTGMQNLAR
EEEEEEECCCCCHHHHHHHHHHHCCHHHCCCCCCEEECCCCEEECCCCCHHHHHHHHHH
RGLDQAVRQAAAAGRPVLGICLGMQLLLAEGDEGGPRPGLGLLEGRVARLPDGLPLPQIG
HHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHCHHHHCCCCCCCCCCC
WNLVEPQRDHPLFAGLPTPFWAYFDHAYAVEGEPPSTALALTDYGRTYPSVVGRGNLLGI
CCCCCCCCCCCEEECCCCHHHHHHCCEEEECCCCCCCEEEECCCCCHHHHHHCCCCEEEE
QFHPEKSSRAGLRMLANWGRMVCDLISTRPST
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA