Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 51893971

GI number: 51893971

Start: 3054449

End: 3055213

Strand: Reverse

Name: hisF

Synonym: STH2833

Alternate gene names: 51893971

Gene position: 3055213-3054449 (Counterclockwise)

Preceding gene: 51893972

Following gene: 51893970

Centisome position: 85.67

GC content: 72.16

Gene sequence:

>765_bases
ATGCCCCTCGCGAAGCGCATCATCCCGTGCCTGGATATCCGGGACGGGCGGGTGGTGAAGAACGTCCAGTTCCACCTGAA
CACCTGGGACGCCGGCGACCCGGTGGCCCTGGCCGCCGAGTACGACCGGCAGGGGGCGGACGAGATCGTCTTTCTGGACA
TCAACGCCTCCTGGGAGGGGCGGTCGGCCACGCTGGACGTCCTGAGCCGGGCCGCCGAGCAGGTCTTCGTGCCCCTGACC
ATCGGCGGGGGGGTGAGCGCCGTCGAGCACGTGAAGGCGTACCTGCGGGCGGGGGCGGACAAGGTGTCGGTCAACACCGC
GGCCGTGCAGCGGCCCGACCTCATCGACGAGATCGCCGACCTGTTCGGCTCCTCCACCCTGGTGGTGGCCATCGACTGCA
AGCGGCGGCCCGAGGGGGGCTGGGAGGTCTACCTGCACGGCGGACGCACGCCCACCGGCATCGACGCCGTCGCCTGGGCC
GAGGAGGCGGCCCGGCGCGGGGCGGGCGAGTTGCTGGTCACGTCGATGGACGCCGACGGCACCCGGCAGGGGTACGACAT
CGCGCTCCACCAGGCCCTGGCCGACGCGGTCGGGGTGCCCGTCATCGCCTCCGGCGGGGCAGGGAGCCCGGAGGACATCC
TCGAAGTTCTGACCGTGGGCCGGGCGGATGCCGCCCTGGCCGCCTCGATCTTCCACAGCGGCAGGCACACGGTGGGGGAG
GTGAAGGCCTTCCTCCGGGAGAGGGGGGTGCTGGTACGGTCATGA

Upstream 100 bases:

>100_bases
CATCATCGGCAGGGCGCTCTACACCGGCGCGGTGGACCTGGCGGAGGCCCTGTCGCTCTGCGGAGAGACCCGTGACACGT
GATAAGGAGGCGCTGGCACC

Downstream 100 bases:

>100_bases
CGGAGACCTTGACGCTGAAGTGGGGCCCGGACGGGCTGATTCCGGCCGTCGTGCAGGACGTGCGGACCGGGCAGGTGCTG
ATGCAGGCGTACATGAACGA

Product: imidazole glycerol phosphate synthase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MPLAKRIIPCLDIRDGRVVKNVQFHLNTWDAGDPVALAAEYDRQGADEIVFLDINASWEGRSATLDVLSRAAEQVFVPLT
IGGGVSAVEHVKAYLRAGADKVSVNTAAVQRPDLIDEIADLFGSSTLVVAIDCKRRPEGGWEVYLHGGRTPTGIDAVAWA
EEAARRGAGELLVTSMDADGTRQGYDIALHQALADAVGVPVIASGGAGSPEDILEVLTVGRADAALAASIFHSGRHTVGE
VKAFLRERGVLVRS

Sequences:

>Translated_254_residues
MPLAKRIIPCLDIRDGRVVKNVQFHLNTWDAGDPVALAAEYDRQGADEIVFLDINASWEGRSATLDVLSRAAEQVFVPLT
IGGGVSAVEHVKAYLRAGADKVSVNTAAVQRPDLIDEIADLFGSSTLVVAIDCKRRPEGGWEVYLHGGRTPTGIDAVAWA
EEAARRGAGELLVTSMDADGTRQGYDIALHQALADAVGVPVIASGGAGSPEDILEVLTVGRADAALAASIFHSGRHTVGE
VKAFLRERGVLVRS
>Mature_253_residues
PLAKRIIPCLDIRDGRVVKNVQFHLNTWDAGDPVALAAEYDRQGADEIVFLDINASWEGRSATLDVLSRAAEQVFVPLTI
GGGVSAVEHVKAYLRAGADKVSVNTAAVQRPDLIDEIADLFGSSTLVVAIDCKRRPEGGWEVYLHGGRTPTGIDAVAWAE
EAARRGAGELLVTSMDADGTRQGYDIALHQALADAVGVPVIASGGAGSPEDILEVLTVGRADAALAASIFHSGRHTVGEV
KAFLRERGVLVRS

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=44.53125, Blast_Score=197, Evalue=4e-52,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=28.3333333333333, Blast_Score=83, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=35.4632587859425, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_SYMTH (Q67KI0)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076662.1
- ProteinModelPortal:   Q67KI0
- SMR:   Q67KI0
- GeneID:   2979879
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2833
- NMPDR:   fig|292459.1.peg.2708
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- BioCyc:   STHE292459:STH2833-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26872; Mature: 26740

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 12-12 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAKRIIPCLDIRDGRVVKNVQFHLNTWDAGDPVALAAEYDRQGADEIVFLDINASWEG
CCCHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEECCCCCC
RSATLDVLSRAAEQVFVPLTIGGGVSAVEHVKAYLRAGADKVSVNTAAVQRPDLIDEIAD
CHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHH
LFGSSTLVVAIDCKRRPEGGWEVYLHGGRTPTGIDAVAWAEEAARRGAGELLVTSMDADG
HHCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TRQGYDIALHQALADAVGVPVIASGGAGSPEDILEVLTVGRADAALAASIFHSGRHTVGE
CCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
VKAFLRERGVLVRS
HHHHHHHCCCEECC
>Mature Secondary Structure 
PLAKRIIPCLDIRDGRVVKNVQFHLNTWDAGDPVALAAEYDRQGADEIVFLDINASWEG
CCHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCEEEEECCCCCCCCEEEEEEECCCCCC
RSATLDVLSRAAEQVFVPLTIGGGVSAVEHVKAYLRAGADKVSVNTAAVQRPDLIDEIAD
CHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHH
LFGSSTLVVAIDCKRRPEGGWEVYLHGGRTPTGIDAVAWAEEAARRGAGELLVTSMDADG
HHCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCC
TRQGYDIALHQALADAVGVPVIASGGAGSPEDILEVLTVGRADAALAASIFHSGRHTVGE
CCCCHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHH
VKAFLRERGVLVRS
HHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA