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The map label for this gene is mdh

Identifier: 51893681

GI number: 51893681

Start: 2728872

End: 2729819

Strand: Reverse

Name: mdh

Synonym: STH2543

Alternate gene names: 51893681

Gene position: 2729819-2728872 (Counterclockwise)

Preceding gene: 51893682

Following gene: 51893680

Centisome position: 76.55

GC content: 67.62

Gene sequence:

>948_bases
ATGAAGCGCAAGAAGATCGCGATTGTGGGCTCCGGGTTCACCGGCCAGGGAACCGCCCTGTTTGCGGCCGCCCGCGACCT
GGGCGACATCGTGCTCGTCGACCTGCCCGCCCGGGAGAACTACGCCAAGGGCGTGGCCCTGGACATGATGGAGGCCATGC
CCGTCTACGGCAGTGACACCCGCCTGGTCGGCACCTCGGACTACGCCGAGATCGCCGGCGCTGACGTGGTCGTGATCACC
GCCGGCGTCCCCCGCAAGCCCGGCATGAGCCGGGAGGACCTGGTCAACACCAACGCCGGCATCGTCAAGGATGTCGCGGA
GAAGGTGGCCCGGTACGCCCCCGACTCGGTGATCATCGTCCTGACCAACCCCGTCGACTCCATGACCTACGTGGCCCTGA
AGGCCTCGGGCTTCCCCAAGAACCGGGTGATCGGCCAGTCCGGCGTGCTGGACACCGCCCGCTTCCGCACCTTCCTGGCC
AATGCGATCGGCTGCTCGTTCCAGGACGTCGTCGGCTGCGTGCTCGGCGGCCACGGCGATGACATGGTGCCGCTGGTTCG
GTACACCTCTGCCGGCGGCATCCCCGTGGAGAAGCTGCTGCCCAAGGAGACCATCGACGCCATCGTCGAACGGACCCGCA
AGGGCGGCGGCGAGATCGTCAACCTCATGGGCACCTCGGCCGGCTACGCCCCGGGCATGGCCCTGGTGGAGATGATCGAC
GCCATTCTGAACGACCGCAAGCGCATCCTGCCCTCGATCGCCTACCTGGAGGGCGAGTACGGGTACACCGACATGTGCCT
GGGCGTGATGACCGTCCTGGGCGGGGGCGGCCTCGAGAAGGTCATCGAGCTCGACCTCACCGACGAGGAGAAGGTGGCGC
TGGACAAGGGCGCCCAGGGCGTCAGGAACCTGATCGAGATGCTGAAGGCCGGCATCCTGGCGCAGTAG

Upstream 100 bases:

>100_bases
GCTCACCGAGGGCGCGACCGAGGTGAAGTGCTCCGAGTTCGGCCGGCTCATCATCGCGAACATGTAGACCGACTGCGGCA
AGGAAAGGGGCAGAGACCGG

Downstream 100 bases:

>100_bases
GTCGATCGCATCCAACAGGGGGAGGAAGGGGACTGTGTCACAGCTGAAAGCCACCCTGGTTCGGAAGATCGAGGAGCATC
GCCCGCGGACCCAGCGGCTG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKRKKIAIVGSGFTGQGTALFAAARDLGDIVLVDLPARENYAKGVALDMMEAMPVYGSDTRLVGTSDYAEIAGADVVVIT
AGVPRKPGMSREDLVNTNAGIVKDVAEKVARYAPDSVIIVLTNPVDSMTYVALKASGFPKNRVIGQSGVLDTARFRTFLA
NAIGCSFQDVVGCVLGGHGDDMVPLVRYTSAGGIPVEKLLPKETIDAIVERTRKGGGEIVNLMGTSAGYAPGMALVEMID
AILNDRKRILPSIAYLEGEYGYTDMCLGVMTVLGGGGLEKVIELDLTDEEKVALDKGAQGVRNLIEMLKAGILAQ

Sequences:

>Translated_315_residues
MKRKKIAIVGSGFTGQGTALFAAARDLGDIVLVDLPARENYAKGVALDMMEAMPVYGSDTRLVGTSDYAEIAGADVVVIT
AGVPRKPGMSREDLVNTNAGIVKDVAEKVARYAPDSVIIVLTNPVDSMTYVALKASGFPKNRVIGQSGVLDTARFRTFLA
NAIGCSFQDVVGCVLGGHGDDMVPLVRYTSAGGIPVEKLLPKETIDAIVERTRKGGGEIVNLMGTSAGYAPGMALVEMID
AILNDRKRILPSIAYLEGEYGYTDMCLGVMTVLGGGGLEKVIELDLTDEEKVALDKGAQGVRNLIEMLKAGILAQ
>Mature_315_residues
MKRKKIAIVGSGFTGQGTALFAAARDLGDIVLVDLPARENYAKGVALDMMEAMPVYGSDTRLVGTSDYAEIAGADVVVIT
AGVPRKPGMSREDLVNTNAGIVKDVAEKVARYAPDSVIIVLTNPVDSMTYVALKASGFPKNRVIGQSGVLDTARFRTFLA
NAIGCSFQDVVGCVLGGHGDDMVPLVRYTSAGGIPVEKLLPKETIDAIVERTRKGGGEIVNLMGTSAGYAPGMALVEMID
AILNDRKRILPSIAYLEGEYGYTDMCLGVMTVLGGGGLEKVIELDLTDEEKVALDKGAQGVRNLIEMLKAGILAQ

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=318, Percent_Identity=33.6477987421384, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI221136809, Length=318, Percent_Identity=33.6477987421384, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=31.4465408805031, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI5031857, Length=305, Percent_Identity=33.1147540983607, Blast_Score=168, Evalue=5e-42,
Organism=Homo sapiens, GI260099723, Length=305, Percent_Identity=33.1147540983607, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=32.2784810126582, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=32.2784810126582, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI9257228, Length=309, Percent_Identity=31.3915857605178, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI4504973, Length=309, Percent_Identity=31.3915857605178, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI260099727, Length=224, Percent_Identity=35.2678571428571, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI260099725, Length=213, Percent_Identity=36.150234741784, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI207028494, Length=187, Percent_Identity=33.1550802139037, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI21735621, Length=255, Percent_Identity=31.3725490196078, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI103472011, Length=311, Percent_Identity=26.6881028938907, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=29.6703296703297, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789632, Length=290, Percent_Identity=31.7241379310345, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=283, Percent_Identity=28.6219081272085, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17554310, Length=173, Percent_Identity=39.3063583815029, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320125, Length=284, Percent_Identity=30.6338028169014, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322765, Length=150, Percent_Identity=34.6666666666667, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324446, Length=248, Percent_Identity=29.4354838709677, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=318, Percent_Identity=29.874213836478, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=29.1390728476821, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24647881, Length=224, Percent_Identity=33.9285714285714, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24663599, Length=238, Percent_Identity=32.7731092436975, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24663595, Length=240, Percent_Identity=31.25, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_SYMTH (Q67LB8)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076372.1
- ProteinModelPortal:   Q67LB8
- SMR:   Q67LB8
- GeneID:   2979396
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2543
- NMPDR:   fig|292459.1.peg.2433
- HOGENOM:   HBG566126
- OMA:   AMTYTVF
- ProtClustDB:   PRK06223
- BioCyc:   STHE292459:STH2543-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33229; Mature: 33229

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: ACT_SITE 178-178 BINDING 34-34 BINDING 85-85 BINDING 91-91 BINDING 98-98 BINDING 123-123 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKKIAIVGSGFTGQGTALFAAARDLGDIVLVDLPARENYAKGVALDMMEAMPVYGSDT
CCCCEEEEEECCCCCCCCEEHEEHHCCCCEEEEECCCCCCHHCCHHHHHHHHCCCCCCCC
RLVGTSDYAEIAGADVVVITAGVPRKPGMSREDLVNTNAGIVKDVAEKVARYAPDSVIIV
EEECCCCHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
LTNPVDSMTYVALKASGFPKNRVIGQSGVLDTARFRTFLANAIGCSFQDVVGCVLGGHGD
EECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
DMVPLVRYTSAGGIPVEKLLPKETIDAIVERTRKGGGEIVNLMGTSAGYAPGMALVEMID
CCCHHEEECCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHH
AILNDRKRILPSIAYLEGEYGYTDMCLGVMTVLGGGGLEKVIELDLTDEEKVALDKGAQG
HHHCCHHHHCCHHHEECCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHCCHHH
VRNLIEMLKAGILAQ
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRKKIAIVGSGFTGQGTALFAAARDLGDIVLVDLPARENYAKGVALDMMEAMPVYGSDT
CCCCEEEEEECCCCCCCCEEHEEHHCCCCEEEEECCCCCCHHCCHHHHHHHHCCCCCCCC
RLVGTSDYAEIAGADVVVITAGVPRKPGMSREDLVNTNAGIVKDVAEKVARYAPDSVIIV
EEECCCCHHHHCCCCEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHCCCCEEEE
LTNPVDSMTYVALKASGFPKNRVIGQSGVLDTARFRTFLANAIGCSFQDVVGCVLGGHGD
EECCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
DMVPLVRYTSAGGIPVEKLLPKETIDAIVERTRKGGGEIVNLMGTSAGYAPGMALVEMID
CCCHHEEECCCCCCCHHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHH
AILNDRKRILPSIAYLEGEYGYTDMCLGVMTVLGGGGLEKVIELDLTDEEKVALDKGAQG
HHHCCHHHHCCHHHEECCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCHHHHHHCCHHH
VRNLIEMLKAGILAQ
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA