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The map label for this gene is proB

Identifier: 51893678

GI number: 51893678

Start: 2724430

End: 2725218

Strand: Reverse

Name: proB

Synonym: STH2540

Alternate gene names: 51893678

Gene position: 2725218-2724430 (Counterclockwise)

Preceding gene: 51893679

Following gene: 51893677

Centisome position: 76.42

GC content: 70.34

Gene sequence:

>789_bases
TTGCGTGAACGGCTGAGGCACTGCAAGCGGGTAATTATCAAGGTAGGGACATCGACGCTGACCCATCCCGGCGGCCATCT
GCACCTGGGCCGCATGGAGGCGCTGGTGCGCCAGATCGCCGACCTGCACTTCGAGGGGCGGCAGGTGATCCTCGTGACCT
CGGGGGCGGTGGGTGCGGGGCTGGGCCGGCTGGGCCTTGCCGAGCGGCCCGCGGAGGTGGCGGCGAAACAGGCCCTCGCG
GCGGTGGGACAGGGGCTCCTGATGCAGCGGTACGAGGGGCTCTTCAGCGAGTACGGCCTGGTGGTCGGCCAGGTGCTGCT
CACCCGGGAGGACCTGGAGGATCCGGACCGTCGCGCCTCCTCCGCCCAGGTGATGGAGCGGCTCCTCGCCTGGGGGGTGA
TCCCCATCGTCAACGAGAACGACACCGTGACCAGCGAGGAGATCCGGGTGGGCGACAACGACACGCTGTCCGCGCGGGTG
GCTGCGCTGGTCCGTGCGGATCTGCTGATCCTGCTCTCCGATGTGGACGGGCTGTACCCGGCGGATCCCCATCTGCACCC
GGGGCTTTCGCCGATCCCGTGGGTCTCGCCGGACGACGACCTGGACCGTTTCGCCGGCGGGCCCGGCAGCGCCAACGGCA
CGGGGGGCATGGTGACCAAGGTCGCGGCCGCCCGCATCTGCGCCGAACACGGGATCCCGATGGTGCTGGCCTGCGGCGAG
CGGCCCGACGTCCTCAGGCAGATCCTGGCCGGTGAGGAGATCGGCACACTCTTCAGTCGGGAGGGGTAG

Upstream 100 bases:

>100_bases
CGAGGCAGCCGCCGCTCAGGCTGGTGGTACCACGGGAGAACACCCCGTCCGGCATGGACGGGGTTATTCTTTTTGGACGA
CCCGTAGGGAGGCGGCGGGT

Downstream 100 bases:

>100_bases
ACGGTGGATGTGCGGAGCATGGCCCGGCAGGCCCGGGCGGCCCAGCGGCAACTGGAGCTGACGTCGCCGGAGGAGCGCAA
CGCGGCCCTGGAGGCGATCG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRERLRHCKRVIIKVGTSTLTHPGGHLHLGRMEALVRQIADLHFEGRQVILVTSGAVGAGLGRLGLAERPAEVAAKQALA
AVGQGLLMQRYEGLFSEYGLVVGQVLLTREDLEDPDRRASSAQVMERLLAWGVIPIVNENDTVTSEEIRVGDNDTLSARV
AALVRADLLILLSDVDGLYPADPHLHPGLSPIPWVSPDDDLDRFAGGPGSANGTGGMVTKVAAARICAEHGIPMVLACGE
RPDVLRQILAGEEIGTLFSREG

Sequences:

>Translated_262_residues
MRERLRHCKRVIIKVGTSTLTHPGGHLHLGRMEALVRQIADLHFEGRQVILVTSGAVGAGLGRLGLAERPAEVAAKQALA
AVGQGLLMQRYEGLFSEYGLVVGQVLLTREDLEDPDRRASSAQVMERLLAWGVIPIVNENDTVTSEEIRVGDNDTLSARV
AALVRADLLILLSDVDGLYPADPHLHPGLSPIPWVSPDDDLDRFAGGPGSANGTGGMVTKVAAARICAEHGIPMVLACGE
RPDVLRQILAGEEIGTLFSREG
>Mature_262_residues
MRERLRHCKRVIIKVGTSTLTHPGGHLHLGRMEALVRQIADLHFEGRQVILVTSGAVGAGLGRLGLAERPAEVAAKQALA
AVGQGLLMQRYEGLFSEYGLVVGQVLLTREDLEDPDRRASSAQVMERLLAWGVIPIVNENDTVTSEEIRVGDNDTLSARV
AALVRADLLILLSDVDGLYPADPHLHPGLSPIPWVSPDDDLDRFAGGPGSANGTGGMVTKVAAARICAEHGIPMVLACGE
RPDVLRQILAGEEIGTLFSREG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=291, Percent_Identity=34.7079037800687, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI21361368, Length=293, Percent_Identity=34.4709897610922, Blast_Score=144, Evalue=7e-35,
Organism=Escherichia coli, GI1786437, Length=255, Percent_Identity=45.4901960784314, Blast_Score=204, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17569969, Length=273, Percent_Identity=36.996336996337, Blast_Score=163, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17569967, Length=273, Percent_Identity=36.996336996337, Blast_Score=163, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6321822, Length=243, Percent_Identity=41.5637860082305, Blast_Score=174, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6320506, Length=239, Percent_Identity=41.0041841004184, Blast_Score=168, Evalue=8e-43,
Organism=Drosophila melanogaster, GI21357643, Length=290, Percent_Identity=34.1379310344828, Blast_Score=164, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_SYMTH (Q67LC1)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_076369.1
- ProteinModelPortal:   Q67LC1
- SMR:   Q67LC1
- GeneID:   2979426
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH2540
- NMPDR:   fig|292459.1.peg.2430
- HOGENOM:   HBG507643
- OMA:   LINTIDP
- ProtClustDB:   PRK12314
- BioCyc:   STHE292459:STH2540-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 28007; Mature: 28007

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERLRHCKRVIIKVGTSTLTHPGGHLHLGRMEALVRQIADLHFEGRQVILVTSGAVGAG
CHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCC
LGRLGLAERPAEVAAKQALAAVGQGLLMQRYEGLFSEYGLVVGQVLLTREDLEDPDRRAS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SAQVMERLLAWGVIPIVNENDTVTSEEIRVGDNDTLSARVAALVRADLLILLSDVDGLYP
HHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
ADPHLHPGLSPIPWVSPDDDLDRFAGGPGSANGTGGMVTKVAAARICAEHGIPMVLACGE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
RPDVLRQILAGEEIGTLFSREG
CHHHHHHHHCCCHHHHHHCCCC
>Mature Secondary Structure
MRERLRHCKRVIIKVGTSTLTHPGGHLHLGRMEALVRQIADLHFEGRQVILVTSGAVGAG
CHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEECCCHHCC
LGRLGLAERPAEVAAKQALAAVGQGLLMQRYEGLFSEYGLVVGQVLLTREDLEDPDRRAS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
SAQVMERLLAWGVIPIVNENDTVTSEEIRVGDNDTLSARVAALVRADLLILLSDVDGLYP
HHHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
ADPHLHPGLSPIPWVSPDDDLDRFAGGPGSANGTGGMVTKVAAARICAEHGIPMVLACGE
CCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
RPDVLRQILAGEEIGTLFSREG
CHHHHHHHHCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA