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The map label for this gene is metB [H]

Identifier: 51893501

GI number: 51893501

Start: 2553233

End: 2554312

Strand: Reverse

Name: metB [H]

Synonym: STH2363

Alternate gene names: 51893501

Gene position: 2554312-2553233 (Counterclockwise)

Preceding gene: 51893502

Following gene: 51893500

Centisome position: 71.63

GC content: 71.39

Gene sequence:

>1080_bases
ATGAAACTGGACACGGTGCTCGTCCACGCAGGGGTCAGGCGTGACCCGGCCTATGGCGCCGTCTCGGTGCCGGTCTACCA
ATCGGCGACCTTTCAGCATCCGGCCCTGGGCGAGTCGACGGGCTACGATTACAGCCGCAGCGGCAACCCGACCCGGGCGG
CCCTGGAGGAAGCGCTTGCCCGGGCGGAGGGGGGCGCCCGGGCGCTCGCCTTTGCCTCGGGCATGGCGGCACTGACCTGT
GCACTGGGGCTCTTCGGGCCCGGCGACCACCTGGTCGTCACCGAGGACTTGTACGGCGGCACCTACCGGCTGTTGGAGCA
GGTGCTGGCCCTGCCGCACACCTACGCGGACACGGCGGATCTGGACGCGGTGCGGGCGGCGATCCGGCCCGACACCCGGG
CGGTCCTCGTCGAGTCCCTCACCAACCCGCGCATGAAGCGGGCGGATGTCGCCGCCCTGGCCGGTCTCTGCCGGGCGCAC
GGCCTGCTGCTGATCGTCGACAACACCTTCCTCACGCCCTGGCTCTGCCGCCCGCTGGAGCTGGGCGCGGACATCGTCGT
CCACAGCGCCACCAAGTACCTGGCCGGCCACAACGACGTGGTGGCCGGGGCGCTGATCACCCGGGACACCGCCCTGGGGG
ACCGGCTCTCCTTTCTCCAGAACGCGGTGGGCTCGGTGCTGGGGCCGCAGGACAGCTGGCTGGTGCTGCGCGGGCTGAAG
ACCCTCGCCCTGCGGATGGAGCGCCACCAGCAGAACGCGGCGCGCATCGCCGCCTGGCTCCGGGAGCACCCGCGGGTCGA
AGAGGTGCTCTACCCGGGCGTGGGCGGGATGCTCTCCTTCACCGTGACGGAGCCCTCTCTGGTGCCGCAGGTGCTGCGCC
GGGTGAAGCTGATCCTGTTCGCCGAGTCGCTGGGGGGCGTGGAGAGCCTGATCACCTTCCCCTGGACCCAGACCCACGCC
GACATGCCGGAGGAGGTGCGGCGGCGGCTGGGCATCACGGACCGGCTGCTGCGGCTCTCGGTGGGCATCGAGGACGCCGA
CGACCTGATCGCCGATCTTGCTCAGGCGCTGGAGGGATAG

Upstream 100 bases:

>100_bases
GACGGCCATCGTCGACGCGCGATCGCTGCCGTTCACCTGCTGGCGATGGCCCGGAAGGGCCCGCTTCGGGTGCCGATTCT
TCCATCCTGGGGGGAGAACC

Downstream 100 bases:

>100_bases
AGCCTTGGAGTTTGGGACGCGGGTTCTGCACACGGGGCGCGAGGTGGACTCCGTCACGGGCGCGGCCGCCGTGCCCATCT
ACCAGGTTTCCACGTTCCGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKLDTVLVHAGVRRDPAYGAVSVPVYQSATFQHPALGESTGYDYSRSGNPTRAALEEALARAEGGARALAFASGMAALTC
ALGLFGPGDHLVVTEDLYGGTYRLLEQVLALPHTYADTADLDAVRAAIRPDTRAVLVESLTNPRMKRADVAALAGLCRAH
GLLLIVDNTFLTPWLCRPLELGADIVVHSATKYLAGHNDVVAGALITRDTALGDRLSFLQNAVGSVLGPQDSWLVLRGLK
TLALRMERHQQNAARIAAWLREHPRVEEVLYPGVGGMLSFTVTEPSLVPQVLRRVKLILFAESLGGVESLITFPWTQTHA
DMPEEVRRRLGITDRLLRLSVGIEDADDLIADLAQALEG

Sequences:

>Translated_359_residues
MKLDTVLVHAGVRRDPAYGAVSVPVYQSATFQHPALGESTGYDYSRSGNPTRAALEEALARAEGGARALAFASGMAALTC
ALGLFGPGDHLVVTEDLYGGTYRLLEQVLALPHTYADTADLDAVRAAIRPDTRAVLVESLTNPRMKRADVAALAGLCRAH
GLLLIVDNTFLTPWLCRPLELGADIVVHSATKYLAGHNDVVAGALITRDTALGDRLSFLQNAVGSVLGPQDSWLVLRGLK
TLALRMERHQQNAARIAAWLREHPRVEEVLYPGVGGMLSFTVTEPSLVPQVLRRVKLILFAESLGGVESLITFPWTQTHA
DMPEEVRRRLGITDRLLRLSVGIEDADDLIADLAQALEG
>Mature_359_residues
MKLDTVLVHAGVRRDPAYGAVSVPVYQSATFQHPALGESTGYDYSRSGNPTRAALEEALARAEGGARALAFASGMAALTC
ALGLFGPGDHLVVTEDLYGGTYRLLEQVLALPHTYADTADLDAVRAAIRPDTRAVLVESLTNPRMKRADVAALAGLCRAH
GLLLIVDNTFLTPWLCRPLELGADIVVHSATKYLAGHNDVVAGALITRDTALGDRLSFLQNAVGSVLGPQDSWLVLRGLK
TLALRMERHQQNAARIAAWLREHPRVEEVLYPGVGGMLSFTVTEPSLVPQVLRRVKLILFAESLGGVESLITFPWTQTHA
DMPEEVRRRLGITDRLLRLSVGIEDADDLIADLAQALEG

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=42.5925925925926, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=39.1534391534392, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=38.1962864721485, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=43.3070866141732, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=27.8772378516624, Blast_Score=148, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17560056, Length=358, Percent_Identity=43.5754189944134, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17538055, Length=358, Percent_Identity=43.5754189944134, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=35.3099730458221, Blast_Score=224, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=41.038961038961, Blast_Score=262, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6321254, Length=401, Percent_Identity=34.1645885286783, Blast_Score=202, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=433, Percent_Identity=33.0254041570439, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=28.7179487179487, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=277, Percent_Identity=32.129963898917, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922554, Length=379, Percent_Identity=46.4379947229551, Blast_Score=315, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 38736; Mature: 38736

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDTVLVHAGVRRDPAYGAVSVPVYQSATFQHPALGESTGYDYSRSGNPTRAALEEALA
CCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RAEGGARALAFASGMAALTCALGLFGPGDHLVVTEDLYGGTYRLLEQVLALPHTYADTAD
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCHHHHHHHHHHCCCCCCCCCH
LDAVRAAIRPDTRAVLVESLTNPRMKRADVAALAGLCRAHGLLLIVDNTFLTPWLCRPLE
HHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHCCHHH
LGADIVVHSATKYLAGHNDVVAGALITRDTALGDRLSFLQNAVGSVLGPQDSWLVLRGLK
CCCCCEEEHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLALRMERHQQNAARIAAWLREHPRVEEVLYPGVGGMLSFTVTEPSLVPQVLRRVKLILF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
AESLGGVESLITFPWTQTHADMPEEVRRRLGITDRLLRLSVGIEDADDLIADLAQALEG
HHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLDTVLVHAGVRRDPAYGAVSVPVYQSATFQHPALGESTGYDYSRSGNPTRAALEEALA
CCCCEEEEECCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
RAEGGARALAFASGMAALTCALGLFGPGDHLVVTEDLYGGTYRLLEQVLALPHTYADTAD
HCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCHHHHHHHHHHCCCCCCCCCH
LDAVRAAIRPDTRAVLVESLTNPRMKRADVAALAGLCRAHGLLLIVDNTFLTPWLCRPLE
HHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHCCHHH
LGADIVVHSATKYLAGHNDVVAGALITRDTALGDRLSFLQNAVGSVLGPQDSWLVLRGLK
CCCCCEEEHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLALRMERHQQNAARIAAWLREHPRVEEVLYPGVGGMLSFTVTEPSLVPQVLRRVKLILF
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHH
AESLGGVESLITFPWTQTHADMPEEVRRRLGITDRLLRLSVGIEDADDLIADLAQALEG
HHHHCCHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]