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The map label for this gene is hmeA [H]

Identifier: 51893221

GI number: 51893221

Start: 2219303

End: 2220073

Strand: Reverse

Name: hmeA [H]

Synonym: STH2083

Alternate gene names: 51893221

Gene position: 2220073-2219303 (Counterclockwise)

Preceding gene: 51893222

Following gene: 51893220

Centisome position: 62.25

GC content: 69.13

Gene sequence:

>771_bases
GTGCAGCCTTCGGCGGCCCTGCGTGGGCACCTCCCCGAGACCCTGGCACGGTCCTTGCATCCATCTCCGGGTGAAGTCCA
CAGATCCAGCAAGGGAGTGACCCCCATGACCGCAGCAGCCCGGGATCCCGGCTCCCGGCACGAGCCCTACTGGGTGATGG
TCATCGACCTGCAGAAGTGCATCGGCTGCGACACCTGCACCGTCTCCTGCAAGGCCGAAAACCGCACCCCGCCGGGCGTC
AGCTACAACGTTGTCCTGGAAATGGAGCTCGGCGAGTACCCCAACACCCGGCGGGCGTACGTGCCCCGGCCCTGCATGCA
GTGCGACAGTCCGCCCTGCGCCGGCGTCTGCCCGGTGGGCGCCACCTACAAGGGCGAGAGCGGCATTGTCGTCATCGACG
ACGACCGGTGCATCGGCTGCCGGTACTGCATCGCCGCCTGCCCCTACGGCGCCCGGTCCTTCGACTTCGGCGAGAACTAC
GACGAGATGCTGGGCTACACCGAGGTCCAGGCCCCCGAGTATGGGGTCGACCGGGGGCCGCGCCGCAGGGGCGAGACGCC
CATCGGCACGGTGCGCAAGTGCACGTTCTGCCTGCACCGCATCCACCGGGGCGAGGAGCCCGCCTGCGTGGAGACCTGCA
TCGGCGACGCCCGGTACTTTGGCGACCTGAACGACCCCAACTCGGTGGTGGCCAAGCTGGCCGCCAGCCCGCGGGCCTTC
CGGCTGAAGGAGGAGCTGGGCACGCAGCCCCGGGTCATCTACCTGAAGTAG

Upstream 100 bases:

>100_bases
CACAGCGCCGGGAGTGACAAAGTGTCACTCCCGGCGTCGCGCGCCTCTGGAGGTATGGCCCGGACCGTTCCGTGGCCCCT
GGACCCGATTCGCCGGGCAT

Downstream 100 bases:

>100_bases
GAGGAGGGAACGCTCGATGTCCGCACTGACCCGAGAAGAGGCGGCCCTGCGGCTCGCCGACCAGCGCCCGTCGCCCGGGC
CGGGCAGGCTGCTCCGAGCG

Product: molybdopterin oxidoreductase iron-sulfur binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MQPSAALRGHLPETLARSLHPSPGEVHRSSKGVTPMTAAARDPGSRHEPYWVMVIDLQKCIGCDTCTVSCKAENRTPPGV
SYNVVLEMELGEYPNTRRAYVPRPCMQCDSPPCAGVCPVGATYKGESGIVVIDDDRCIGCRYCIAACPYGARSFDFGENY
DEMLGYTEVQAPEYGVDRGPRRRGETPIGTVRKCTFCLHRIHRGEEPACVETCIGDARYFGDLNDPNSVVAKLAASPRAF
RLKEELGTQPRVIYLK

Sequences:

>Translated_256_residues
MQPSAALRGHLPETLARSLHPSPGEVHRSSKGVTPMTAAARDPGSRHEPYWVMVIDLQKCIGCDTCTVSCKAENRTPPGV
SYNVVLEMELGEYPNTRRAYVPRPCMQCDSPPCAGVCPVGATYKGESGIVVIDDDRCIGCRYCIAACPYGARSFDFGENY
DEMLGYTEVQAPEYGVDRGPRRRGETPIGTVRKCTFCLHRIHRGEEPACVETCIGDARYFGDLNDPNSVVAKLAASPRAF
RLKEELGTQPRVIYLK
>Mature_256_residues
MQPSAALRGHLPETLARSLHPSPGEVHRSSKGVTPMTAAARDPGSRHEPYWVMVIDLQKCIGCDTCTVSCKAENRTPPGV
SYNVVLEMELGEYPNTRRAYVPRPCMQCDSPPCAGVCPVGATYKGESGIVVIDDDRCIGCRYCIAACPYGARSFDFGENY
DEMLGYTEVQAPEYGVDRGPRRRGETPIGTVRKCTFCLHRIHRGEEPACVETCIGDARYFGDLNDPNSVVAKLAASPRAF
RLKEELGTQPRVIYLK

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=204, Percent_Identity=36.2745098039216, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1787872, Length=211, Percent_Identity=33.175355450237, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1787122, Length=211, Percent_Identity=32.7014218009479, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787740, Length=155, Percent_Identity=34.1935483870968, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI226510944, Length=182, Percent_Identity=29.6703296703297, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787478, Length=144, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789370, Length=194, Percent_Identity=29.8969072164948, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787749, Length=203, Percent_Identity=31.5270935960591, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1790326, Length=207, Percent_Identity=31.8840579710145, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082179, Length=162, Percent_Identity=30.8641975308642, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789067, Length=184, Percent_Identity=30.9782608695652, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367245, Length=102, Percent_Identity=37.2549019607843, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787963, Length=117, Percent_Identity=30.7692307692308, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 28135; Mature: 28135

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSAALRGHLPETLARSLHPSPGEVHRSSKGVTPMTAAARDPGSRHEPYWVMVIDLQKC
CCCCCCHHCCCHHHHHHHCCCCCCHHHHCCCCCCCCCHHCCCCCCCCCCEEEEEEEEHHH
IGCDTCTVSCKAENRTPPGVSYNVVLEMELGEYPNTRRAYVPRPCMQCDSPPCAGVCPVG
CCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
ATYKGESGIVVIDDDRCIGCRYCIAACPYGARSFDFGENYDEMLGYTEVQAPEYGVDRGP
CEEECCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCC
RRRGETPIGTVRKCTFCLHRIHRGEEPACVETCIGDARYFGDLNDPNSVVAKLAASPRAF
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCE
RLKEELGTQPRVIYLK
EEHHHHCCCCEEEEEC
>Mature Secondary Structure
MQPSAALRGHLPETLARSLHPSPGEVHRSSKGVTPMTAAARDPGSRHEPYWVMVIDLQKC
CCCCCCHHCCCHHHHHHHCCCCCCHHHHCCCCCCCCCHHCCCCCCCCCCEEEEEEEEHHH
IGCDTCTVSCKAENRTPPGVSYNVVLEMELGEYPNTRRAYVPRPCMQCDSPPCAGVCPVG
CCCCCEEEEEECCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
ATYKGESGIVVIDDDRCIGCRYCIAACPYGARSFDFGENYDEMLGYTEVQAPEYGVDRGP
CEEECCCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCC
RRRGETPIGTVRKCTFCLHRIHRGEEPACVETCIGDARYFGDLNDPNSVVAKLAASPRAF
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHCCCCCE
RLKEELGTQPRVIYLK
EEHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]