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The map label for this gene is mscL

Identifier: 51892529

GI number: 51892529

Start: 1539477

End: 1539917

Strand: Reverse

Name: mscL

Synonym: STH1391

Alternate gene names: 51892529

Gene position: 1539917-1539477 (Counterclockwise)

Preceding gene: 51892530

Following gene: 51892519

Centisome position: 43.18

GC content: 64.85

Gene sequence:

>441_bases
ATGCTGTCTGAGTTCAAGCGGTTCGCGCTGAGGGGAAACGTGCTGGATCTGGCCGTCGGCGTGGTGATCGGGGCGGCGTT
CAACCAGATCGTCAACTCGCTGGTCAACGACGTGATCATGCCACCGCTGGGATTCCTGGTCGGCAAGATGGACTTCTCGA
ACCTGTACCTCAACCTGAGCCTGACGCCCTACGCCTCGCTGGCCGAGGCGCGCGAGGCCGGGGCCCCCGTCATTGCCTAC
GGCCTCTTCCTCAACAACCTGCTCAACTTCCTCATCGTCGCCTTCGCCGTGTTCCTGCTGGTCCGCCAGGTCAACCGGTG
GCGCCCGACGCCCCCGCCCGAGCCGCCCAAGACCAAGGAGTGCCCCTACTGCCTCTCCACGGTGCCCGTCAAGGCGACGC
GGTGTGCCCACTGCACATCTGACCTCACCGCCGAAAGCTAA

Upstream 100 bases:

>100_bases
GCCCGCCGAGCTGGTCCCCCTCTGACCGGGTGCACGACTGTCTGGTGCGCGACCGTTTGTGTTAGACTATATAAACATCT
CTTACAGAGGAGGGGCCGGG

Downstream 100 bases:

>100_bases
CGCAAGGCCCTCCGCCCTGGACCGGGGCGGAGGGCCTTGGGTGTTGGCGAATGCAACGCTTACAGTTCGATCTTCGTCCC
CAGTACCTTCAGGAACTGGG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLSEFKRFALRGNVLDLAVGVVIGAAFNQIVNSLVNDVIMPPLGFLVGKMDFSNLYLNLSLTPYASLAEAREAGAPVIAY
GLFLNNLLNFLIVAFAVFLLVRQVNRWRPTPPPEPPKTKECPYCLSTVPVKATRCAHCTSDLTAES

Sequences:

>Translated_146_residues
MLSEFKRFALRGNVLDLAVGVVIGAAFNQIVNSLVNDVIMPPLGFLVGKMDFSNLYLNLSLTPYASLAEAREAGAPVIAY
GLFLNNLLNFLIVAFAVFLLVRQVNRWRPTPPPEPPKTKECPYCLSTVPVKATRCAHCTSDLTAES
>Mature_146_residues
MLSEFKRFALRGNVLDLAVGVVIGAAFNQIVNSLVNDVIMPPLGFLVGKMDFSNLYLNLSLTPYASLAEAREAGAPVIAY
GLFLNNLLNFLIVAFAVFLLVRQVNRWRPTPPPEPPKTKECPYCLSTVPVKATRCAHCTSDLTAES

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=127, Percent_Identity=47.244094488189, Blast_Score=114, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_SYMTH (Q67PL7)

Other databases:

- EMBL:   AP006840
- RefSeq:   YP_075220.1
- ProteinModelPortal:   Q67PL7
- SMR:   Q67PL7
- GeneID:   2980441
- GenomeReviews:   AP006840_GR
- KEGG:   sth:STH1391
- NMPDR:   fig|292459.1.peg.1335
- HOGENOM:   HBG727604
- OMA:   FATITKS
- ProtClustDB:   PRK00567
- BioCyc:   STHE292459:STH1391-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15956; Mature: 15956

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1289dbd4)-; HASH(0x12818098)-;

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEFKRFALRGNVLDLAVGVVIGAAFNQIVNSLVNDVIMPPLGFLVGKMDFSNLYLNLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEE
LTPYASLAEAREAGAPVIAYGLFLNNLLNFLIVAFAVFLLVRQVNRWRPTPPPEPPKTKE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
CPYCLSTVPVKATRCAHCTSDLTAES
CCCHHHCCCCCHHHHHHHHCCCCCCH
>Mature Secondary Structure
MLSEFKRFALRGNVLDLAVGVVIGAAFNQIVNSLVNDVIMPPLGFLVGKMDFSNLYLNLS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEEEE
LTPYASLAEAREAGAPVIAYGLFLNNLLNFLIVAFAVFLLVRQVNRWRPTPPPEPPKTKE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
CPYCLSTVPVKATRCAHCTSDLTAES
CCCHHHCCCCCHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA