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The map label for this gene is nfnB [C]

Identifier: 51892201

GI number: 51892201

Start: 1175739

End: 1176383

Strand: Reverse

Name: nfnB [C]

Synonym: STH1063

Alternate gene names: 51892201

Gene position: 1176383-1175739 (Counterclockwise)

Preceding gene: 51892202

Following gene: 51892197

Centisome position: 32.99

GC content: 71.78

Gene sequence:

>645_bases
ATGACCGAGTTGCCGCAGAATCCGTGTGACGTCCTCGAAGCGATCGCCCGCCGCCACAGCGTGCGGGAGTTCCGGCCCGA
TCCGGTGTCCGAGCAGGTGCTCCTGCAACTCCTGGAGGCCGCCCGGCAGGCCCCCAGCGCCATGAACCTTCAGCCCTGGG
AGTTCGTGGTGGTCCGCCGACCGGAGACCAAGGCGCGGCTGGCCGCCGCCACCGCAGGGCGGAACGCGCAGGTCGTGCAG
ACGGCGGGAGCATCGGTGGTCTGCCTGGCATCGCTCCGGCAGGCGGACGCCCTGGCGGACCGGATCGAAGGCCAGATCCG
GGCCGGACAGGCGCCGCCCGACCGCGAGGCCATGGTCCGACGGCTGCGGGAGGACCAGGCTTACCGGCAGCTGCTGGTCC
TGCCCAACACCTATACAGCCGTCGGCTTCCTGCTGCTGGCCGCGACCCGGCTCGGGCTCGCGACCCTCTGGATGACCGGC
TTCGACGGCGAGAAGGTCAAGGAGGCCGTCGGCGCGCCGGCGCAGGACTCCCTCTTCGCCGGCGTCGTGGCCGTGGGCTA
CCCGGCGGAGGGATGGCAACAGCCCGCCCGCACCCGCAGGCCCCTGGAGGAGATCTACTCGTTCGAGCGGTTCGGGGAGA
AGTAG

Upstream 100 bases:

>100_bases
CTCCTGGGGTGGGCGGTGCTGCTCCTCTCCTCCATCCCGCTGCGGGGCATAAAGGAGGGCCTCTCCTCCGGAGACTGATC
CCGTGAAGGGAGTTGTGAAC

Downstream 100 bases:

>100_bases
GCCAGCGAAGAGCCCCCGGGTGGCTGTGCGCACCCGGGGGCTGCCCGTTGCCGTCTGCCGGTCCGTCTTCCCTGGGGTGC
CGCCCGGCGCTGCGCCCGGC

Product: NAD(P)H nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTELPQNPCDVLEAIARRHSVREFRPDPVSEQVLLQLLEAARQAPSAMNLQPWEFVVVRRPETKARLAAATAGRNAQVVQ
TAGASVVCLASLRQADALADRIEGQIRAGQAPPDREAMVRRLREDQAYRQLLVLPNTYTAVGFLLLAATRLGLATLWMTG
FDGEKVKEAVGAPAQDSLFAGVVAVGYPAEGWQQPARTRRPLEEIYSFERFGEK

Sequences:

>Translated_214_residues
MTELPQNPCDVLEAIARRHSVREFRPDPVSEQVLLQLLEAARQAPSAMNLQPWEFVVVRRPETKARLAAATAGRNAQVVQ
TAGASVVCLASLRQADALADRIEGQIRAGQAPPDREAMVRRLREDQAYRQLLVLPNTYTAVGFLLLAATRLGLATLWMTG
FDGEKVKEAVGAPAQDSLFAGVVAVGYPAEGWQQPARTRRPLEEIYSFERFGEK
>Mature_213_residues
TELPQNPCDVLEAIARRHSVREFRPDPVSEQVLLQLLEAARQAPSAMNLQPWEFVVVRRPETKARLAAATAGRNAQVVQT
AGASVVCLASLRQADALADRIEGQIRAGQAPPDREAMVRRLREDQAYRQLLVLPNTYTAVGFLLLAATRLGLATLWMTGF
DGEKVKEAVGAPAQDSLFAGVVAVGYPAEGWQQPARTRRPLEEIYSFERFGEK

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23577; Mature: 23446

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELPQNPCDVLEAIARRHSVREFRPDPVSEQVLLQLLEAARQAPSAMNLQPWEFVVVRR
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
PETKARLAAATAGRNAQVVQTAGASVVCLASLRQADALADRIEGQIRAGQAPPDREAMVR
CCHHHHHHHHHCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHH
RLREDQAYRQLLVLPNTYTAVGFLLLAATRLGLATLWMTGFDGEKVKEAVGAPAQDSLFA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHH
GVVAVGYPAEGWQQPARTRRPLEEIYSFERFGEK
HHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TELPQNPCDVLEAIARRHSVREFRPDPVSEQVLLQLLEAARQAPSAMNLQPWEFVVVRR
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEC
PETKARLAAATAGRNAQVVQTAGASVVCLASLRQADALADRIEGQIRAGQAPPDREAMVR
CCHHHHHHHHHCCCCCEEEHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHH
RLREDQAYRQLLVLPNTYTAVGFLLLAATRLGLATLWMTGFDGEKVKEAVGAPAQDSLFA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHH
GVVAVGYPAEGWQQPARTRRPLEEIYSFERFGEK
HHHHCCCCCHHHHCHHHHHCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]