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The map label for this gene is dnaN [H]

Identifier: 51598692

GI number: 51598692

Start: 460484

End: 461644

Strand: Direct

Name: dnaN [H]

Synonym: BG0445

Alternate gene names: 51598692

Gene position: 460484-461644 (Clockwise)

Preceding gene: 51598691

Following gene: 51598693

Centisome position: 50.92

GC content: 26.27

Gene sequence:

>1161_bases
ATGCTAAATAACACATTTTTTATTTGCGAAACAAATCAAATTACAAGTGAAATAGAAAAAGCAAAAGGAATAATATTAAA
CAGAAATATGAATGACATTTGGAGCGCATTGTTAATTGAAGTAAAAAGGTCTAATCTCATAATTAAATCAACAGACAGGA
ACATATTTTTTGAAAGCACAATATCAATTATTTCAGAAACAGATTTTAAGGTATTAATTAACGCCTCAAATTTTTATGAC
GCAGTAAAAGCATTCAGTTTTTACAAGAAAATTAAAATAGTTTTTAATGAGAATAACAGTAAATTGGAAATTATGGGAGA
AGCAAAAGATGAAAAAGAAGAAAAATGTGAAGATCATTTAAAGGAACCTACCTTTTCATACGAAGAAATAGAAAATTACA
ATTATGATATGGTTAATGAAGATTACACTTTTGAAATTGAATTAAAACAAAAATCTTTTAAAAAAATAATAAATAGAATA
GCTTTTTCAGCACATCTTGATGAATCCAAAAATGTATTAAATGGTATATATTTTACAAAAGGTGAAGATTCTAAGTTATT
GCTTGTTTCTACTAATGGCCACAGAATGTCTATTTGCAAAACAGAAGTCATAGTTGAAGAAGATGTTAATTTTATAGTTC
CTGTAAAAATATTTAATTTTTTAAAACACCTTATGTCAGGAGAAGGTATGGTTAAAATAAAGTCTTCGGATAAAAAATTT
TATGTTGAATTTGATAATTATAAAATAGCCTGCAGTTTGATTAGTGGTAATTATCCTGATTACGAGAGCATTATACCTAA
AGAACAAGAAAATAAATCTTTGGTTTCGCTAAGCATTTTAAAAGATAGACTCGCAAGAGTTAATTTATATGTTGATAAGT
CAAAAAAGTTGATTTTAACTTTTTCTGAGCTTCAATTTAAACTTCTTGGAGAAGATTTAATTACTGGAAGAAAAGGTGAA
TTTTTTATTAAAAACCCGAACTATCTGTATGACGGATCAGATGAAGTTGTGGCGATTAATATTTCATATTTTATTGAAGC
TATTAGTGTTTTTGAAACTTCGAAGATAGAAATACAATTTAATTCAGGGAATGTATTGAAATTAAGTGAACCTGAAAACT
TTAATTTTACACACTTGATAATGCCGATGTCTTTAGGTTAA

Upstream 100 bases:

>100_bases
ACTTACTACTATTACTATTATATATATCTAAAAAAACTAAAAATAAAAAACAATAAAAACTTACAAAGATAAAATGTAAT
AAAATATATGAGGAGGCATC

Downstream 100 bases:

>100_bases
AGTGAATTATTAATGTAATTTTAAATATTAAAACGAGCTTTTATAAAGCTTATTTTATATTGTTTTTTGCATTGCGGTAT
AAAAAAATTGTTATGAATGA

Product: DNA polymerase III, subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MLNNTFFICETNQITSEIEKAKGIILNRNMNDIWSALLIEVKRSNLIIKSTDRNIFFESTISIISETDFKVLINASNFYD
AVKAFSFYKKIKIVFNENNSKLEIMGEAKDEKEEKCEDHLKEPTFSYEEIENYNYDMVNEDYTFEIELKQKSFKKIINRI
AFSAHLDESKNVLNGIYFTKGEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKSSDKKF
YVEFDNYKIACSLISGNYPDYESIIPKEQENKSLVSLSILKDRLARVNLYVDKSKKLILTFSELQFKLLGEDLITGRKGE
FFIKNPNYLYDGSDEVVAINISYFIEAISVFETSKIEIQFNSGNVLKLSEPENFNFTHLIMPMSLG

Sequences:

>Translated_386_residues
MLNNTFFICETNQITSEIEKAKGIILNRNMNDIWSALLIEVKRSNLIIKSTDRNIFFESTISIISETDFKVLINASNFYD
AVKAFSFYKKIKIVFNENNSKLEIMGEAKDEKEEKCEDHLKEPTFSYEEIENYNYDMVNEDYTFEIELKQKSFKKIINRI
AFSAHLDESKNVLNGIYFTKGEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKSSDKKF
YVEFDNYKIACSLISGNYPDYESIIPKEQENKSLVSLSILKDRLARVNLYVDKSKKLILTFSELQFKLLGEDLITGRKGE
FFIKNPNYLYDGSDEVVAINISYFIEAISVFETSKIEIQFNSGNVLKLSEPENFNFTHLIMPMSLG
>Mature_386_residues
MLNNTFFICETNQITSEIEKAKGIILNRNMNDIWSALLIEVKRSNLIIKSTDRNIFFESTISIISETDFKVLINASNFYD
AVKAFSFYKKIKIVFNENNSKLEIMGEAKDEKEEKCEDHLKEPTFSYEEIENYNYDMVNEDYTFEIELKQKSFKKIINRI
AFSAHLDESKNVLNGIYFTKGEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKSSDKKF
YVEFDNYKIACSLISGNYPDYESIIPKEQENKSLVSLSILKDRLARVNLYVDKSKKLILTFSELQFKLLGEDLITGRKGE
FFIKNPNYLYDGSDEVVAINISYFIEAISVFETSKIEIQFNSGNVLKLSEPENFNFTHLIMPMSLG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=387, Percent_Identity=20.1550387596899, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44635; Mature: 44635

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNTFFICETNQITSEIEKAKGIILNRNMNDIWSALLIEVKRSNLIIKSTDRNIFFEST
CCCCEEEEEECCHHHHHHHHHCCEEEECCHHHHHHHHHEEECCCCEEEEECCCCEEEEEH
ISIISETDFKVLINASNFYDAVKAFSFYKKIKIVFNENNSKLEIMGEAKDEKEEKCEDHL
HHHHCCCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHH
KEPTFSYEEIENYNYDMVNEDYTFEIELKQKSFKKIINRIAFSAHLDESKNVLNGIYFTK
CCCCCCHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEC
GEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKSSDKKF
CCCCEEEEEECCCCEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCEE
YVEFDNYKIACSLISGNYPDYESIIPKEQENKSLVSLSILKDRLARVNLYVDKSKKLILT
EEEECCEEEEEEEECCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHEEEEEEECCCEEEEE
FSELQFKLLGEDLITGRKGEFFIKNPNYLYDGSDEVVAINISYFIEAISVFETSKIEIQF
EHHHEEEEECHHHHCCCCCCEEEECCCEEECCCCCEEEEEHHHHHHHHHHEECEEEEEEE
NSGNVLKLSEPENFNFTHLIMPMSLG
CCCCEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MLNNTFFICETNQITSEIEKAKGIILNRNMNDIWSALLIEVKRSNLIIKSTDRNIFFEST
CCCCEEEEEECCHHHHHHHHHCCEEEECCHHHHHHHHHEEECCCCEEEEECCCCEEEEEH
ISIISETDFKVLINASNFYDAVKAFSFYKKIKIVFNENNSKLEIMGEAKDEKEEKCEDHL
HHHHCCCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHH
KEPTFSYEEIENYNYDMVNEDYTFEIELKQKSFKKIINRIAFSAHLDESKNVLNGIYFTK
CCCCCCHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHCCEEEEC
GEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKSSDKKF
CCCCEEEEEECCCCEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCEE
YVEFDNYKIACSLISGNYPDYESIIPKEQENKSLVSLSILKDRLARVNLYVDKSKKLILT
EEEECCEEEEEEEECCCCCCHHHCCCCCCCCCCEEEHHHHHHHHHEEEEEEECCCEEEEE
FSELQFKLLGEDLITGRKGEFFIKNPNYLYDGSDEVVAINISYFIEAISVFETSKIEIQF
EHHHEEEEECHHHHCCCCCCEEEECCCEEECCCCCEEEEEHHHHHHHHHHEECEEEEEEE
NSGNVLKLSEPENFNFTHLIMPMSLG
CCCCEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8341609; 9403685; 8359672 [H]