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The map label for this gene is oppA-2 [H]

Identifier: 51598587

GI number: 51598587

Start: 335391

End: 336974

Strand: Direct

Name: oppA-2 [H]

Synonym: BG0330

Alternate gene names: 51598587

Gene position: 335391-336974 (Clockwise)

Preceding gene: 51598586

Following gene: 51598588

Centisome position: 37.09

GC content: 32.26

Gene sequence:

>1584_bases
ATGAAATTACAAAAGTTATTATTTTCAGTAATATTTTTTCTAACTTTTCTTTGTTGTAATAAAGAGGAAAAAAAAGAAGG
AATATCATTTAAAATAAGCCTGGGATCAGAACCAAGCAGTCTTGACCCCCAATTAGCAGACGACAATGTCGGATCAAAAA
TGATAGACACAATGTTTAGAGGGCTTATTACAGGCGATCCTAACACAGGAGGAAACAAACCAGGACTTGCAAAAAGTTGG
GATATTTCTCCTGACGGAACAGTTTACACATTCACCCTAAGAGAAAAAATCATCTGGAGTGATGGGGTTGCAATCACTGC
AGAAGGGATTAGAAAATCTTATCTTAGAATTTTAAATAAAGAAACTGGCTCAAATTATGCTGAAATGGTTAAATCAACCA
TTAAGAATGGTCAAAAATATTTTGATGGACAAGTGTCTGACTCTGAACTTGGAATTAGGGCAATTGACGAAAAAACATTA
GAAATAACACTCGAATCACCAAAACCCTATTTTATCGATATGTTAGTACACCAATCATTTATTCCAATACCGATTCATAT
TGCCGAAAAGTATGGACAAAGCTGGACAAGCCCTGAGAACATAGTTACCAGCGGTCCTTTTAAGTTAAAAGAAAGAATTC
CTAACGAAAAATATGTGGTCGAAAAAAATGATAAGTACTATAACTCAAACCAAGTAGAAGTACAAGAAATTACATTTTAT
ACAACCAATGACAGCTCAACAGCATACAAAATGTATGAAAATAAAGAATTAGATGCAATATTTGGCTCCATACCGCCTGA
TTTAATTAAAGATCTAAAGTTAAGAAGTGATTATTACTCATCAGCTGTTAATGCTATATATTTTTACGCATTCAACACGC
ACATTAAACCATTAGACAATGTTAAAGTTAGAAAAGCCTTAACTCTTGCTATTGATAGAGAAACACTTACCTATAAAGTT
CTTGACAATGGGACTACTCCTACAAGGAGAATAGCCCCTAACTTTAGCTCATATTCTTATGCAAAAAATTTGGAATTATT
TAATCCTGAAATTGCAAAAACCCTTCTAGCTGAAGCTGGTTACCCTAATGGCAATGGATTTCCTATTTTAAAATTAAAAT
ATAATACAAGTGAAGCTCATAAAAAAATTTGTGAATTTATTCAAAACCAATGGAAAAAAATTTTAAACATTGATGTGGAA
CTTGAAAACGAAGAATGGACAACTTACCTAAACACTAGGTCAAATGGAAATTACGAAATAGCAAGAGCAGGGTGGATAGG
CGATTATGCTGATCCTTTAACATTTTTAAGTATATTCACACAAGGATACACACAATTCTCATCTCACAATTATTCAAGCC
CAGAATACAACGAACTTATTAAAAAGTCTGATCTTGAACTTGATCCAATAAAAAGACAAGATATCCTAAGAAAAGCAGAA
GAGATAATTATTGAAAAAGATTTTCCAATAGCCCCAATATATATATATGGAAATAGTTACCTTTTCAGAAATGACAAGTG
GACAGGCCGGAATACCAATATTACAGAAAGATTCGATTTATCTCAACTAAAATTAAAAAAATAA

Upstream 100 bases:

>100_bases
TAATAAATTAAGTAATTTTAAAATTAAGATTTAAAATTAATATCTACGGAGGGGCAATTATAAAATTAAAGTTAACTTTA
AAACGGAGGAATAAAAAATT

Downstream 100 bases:

>100_bases
ATAAATAATCTTTAATTAAATAAAAATCGTCTATGAATTTCTTTTCATAGGCGATTTTTTTATTGACTTTCTTCTAAAAA
GAAGATAGCTTAAAACTTAT

Product: oligopeptide ABC transporter, periplasmic oligopeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MKLQKLLFSVIFFLTFLCCNKEEKKEGISFKISLGSEPSSLDPQLADDNVGSKMIDTMFRGLITGDPNTGGNKPGLAKSW
DISPDGTVYTFTLREKIIWSDGVAITAEGIRKSYLRILNKETGSNYAEMVKSTIKNGQKYFDGQVSDSELGIRAIDEKTL
EITLESPKPYFIDMLVHQSFIPIPIHIAEKYGQSWTSPENIVTSGPFKLKERIPNEKYVVEKNDKYYNSNQVEVQEITFY
TTNDSSTAYKMYENKELDAIFGSIPPDLIKDLKLRSDYYSSAVNAIYFYAFNTHIKPLDNVKVRKALTLAIDRETLTYKV
LDNGTTPTRRIAPNFSSYSYAKNLELFNPEIAKTLLAEAGYPNGNGFPILKLKYNTSEAHKKICEFIQNQWKKILNIDVE
LENEEWTTYLNTRSNGNYEIARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSSPEYNELIKKSDLELDPIKRQDILRKAE
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGRNTNITERFDLSQLKLKK

Sequences:

>Translated_527_residues
MKLQKLLFSVIFFLTFLCCNKEEKKEGISFKISLGSEPSSLDPQLADDNVGSKMIDTMFRGLITGDPNTGGNKPGLAKSW
DISPDGTVYTFTLREKIIWSDGVAITAEGIRKSYLRILNKETGSNYAEMVKSTIKNGQKYFDGQVSDSELGIRAIDEKTL
EITLESPKPYFIDMLVHQSFIPIPIHIAEKYGQSWTSPENIVTSGPFKLKERIPNEKYVVEKNDKYYNSNQVEVQEITFY
TTNDSSTAYKMYENKELDAIFGSIPPDLIKDLKLRSDYYSSAVNAIYFYAFNTHIKPLDNVKVRKALTLAIDRETLTYKV
LDNGTTPTRRIAPNFSSYSYAKNLELFNPEIAKTLLAEAGYPNGNGFPILKLKYNTSEAHKKICEFIQNQWKKILNIDVE
LENEEWTTYLNTRSNGNYEIARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSSPEYNELIKKSDLELDPIKRQDILRKAE
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGRNTNITERFDLSQLKLKK
>Mature_527_residues
MKLQKLLFSVIFFLTFLCCNKEEKKEGISFKISLGSEPSSLDPQLADDNVGSKMIDTMFRGLITGDPNTGGNKPGLAKSW
DISPDGTVYTFTLREKIIWSDGVAITAEGIRKSYLRILNKETGSNYAEMVKSTIKNGQKYFDGQVSDSELGIRAIDEKTL
EITLESPKPYFIDMLVHQSFIPIPIHIAEKYGQSWTSPENIVTSGPFKLKERIPNEKYVVEKNDKYYNSNQVEVQEITFY
TTNDSSTAYKMYENKELDAIFGSIPPDLIKDLKLRSDYYSSAVNAIYFYAFNTHIKPLDNVKVRKALTLAIDRETLTYKV
LDNGTTPTRRIAPNFSSYSYAKNLELFNPEIAKTLLAEAGYPNGNGFPILKLKYNTSEAHKKICEFIQNQWKKILNIDVE
LENEEWTTYLNTRSNGNYEIARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSSPEYNELIKKSDLELDPIKRQDILRKAE
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGRNTNITERFDLSQLKLKK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=484, Percent_Identity=34.7107438016529, Blast_Score=284, Evalue=8e-78,
Organism=Escherichia coli, GI87081878, Length=479, Percent_Identity=35.9081419624217, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1789397, Length=481, Percent_Identity=32.016632016632, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789966, Length=461, Percent_Identity=25.3796095444685, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1787052, Length=475, Percent_Identity=25.4736842105263, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1789887, Length=466, Percent_Identity=25.5364806866953, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1787762, Length=516, Percent_Identity=22.2868217054264, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787551, Length=483, Percent_Identity=22.360248447205, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60393; Mature: 60393

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQKLLFSVIFFLTFLCCNKEEKKEGISFKISLGSEPSSLDPQLADDNVGSKMIDTMFR
CCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
GLITGDPNTGGNKPGLAKSWDISPDGTVYTFTLREKIIWSDGVAITAEGIRKSYLRILNK
HEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCC
ETGSNYAEMVKSTIKNGQKYFDGQVSDSELGIRAIDEKTLEITLESPKPYFIDMLVHQSF
CCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCEEEEEEECCCCCCEEHHHHHCCC
IPIPIHIAEKYGQSWTSPENIVTSGPFKLKERIPNEKYVVEKNDKYYNSNQVEVQEITFY
CCCEEEHHHHHCCCCCCCCCEEECCCHHHHHHCCCCCEEEECCCCEECCCCEEEEEEEEE
TTNDSSTAYKMYENKELDAIFGSIPPDLIKDLKLRSDYYSSAVNAIYFYAFNTHIKPLDN
EECCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCC
VKVRKALTLAIDRETLTYKVLDNGTTPTRRIAPNFSSYSYAKNLELFNPEIAKTLLAEAG
CHHEEEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCC
YPNGNGFPILKLKYNTSEAHKKICEFIQNQWKKILNIDVELENEEWTTYLNTRSNGNYEI
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCEEE
ARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSSPEYNELIKKSDLELDPIKRQDILRKAE
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGRNTNITERFDLSQLKLKK
HHEEECCCCEEEEEEECCEEEEECCCCCCCCCCCCEECCCHHHCCCC
>Mature Secondary Structure
MKLQKLLFSVIFFLTFLCCNKEEKKEGISFKISLGSEPSSLDPQLADDNVGSKMIDTMFR
CCHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
GLITGDPNTGGNKPGLAKSWDISPDGTVYTFTLREKIIWSDGVAITAEGIRKSYLRILNK
HEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCC
ETGSNYAEMVKSTIKNGQKYFDGQVSDSELGIRAIDEKTLEITLESPKPYFIDMLVHQSF
CCCCHHHHHHHHHHHCCHHHCCCCCCCCCCCEEEECCEEEEEEECCCCCCEEHHHHHCCC
IPIPIHIAEKYGQSWTSPENIVTSGPFKLKERIPNEKYVVEKNDKYYNSNQVEVQEITFY
CCCEEEHHHHHCCCCCCCCCEEECCCHHHHHHCCCCCEEEECCCCEECCCCEEEEEEEEE
TTNDSSTAYKMYENKELDAIFGSIPPDLIKDLKLRSDYYSSAVNAIYFYAFNTHIKPLDN
EECCCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCC
VKVRKALTLAIDRETLTYKVLDNGTTPTRRIAPNFSSYSYAKNLELFNPEIAKTLLAEAG
CHHEEEEEEEEECCEEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHCC
YPNGNGFPILKLKYNTSEAHKKICEFIQNQWKKILNIDVELENEEWTTYLNTRSNGNYEI
CCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCEEE
ARAGWIGDYADPLTFLSIFTQGYTQFSSHNYSSPEYNELIKKSDLELDPIKRQDILRKAE
EEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHH
EIIIEKDFPIAPIYIYGNSYLFRNDKWTGRNTNITERFDLSQLKLKK
HHEEECCCCEEEEEEECCEEEEECCCCCCCCCCCCEECCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2821267; 3525163; 11677609; 8202710; 8747465; 10422831; 10438628 [H]