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The map label for this gene is oppA-1 [H]

Identifier: 51598586

GI number: 51598586

Start: 333722

End: 335293

Strand: Direct

Name: oppA-1 [H]

Synonym: BG0329

Alternate gene names: 51598586

Gene position: 333722-335293 (Clockwise)

Preceding gene: 51598582

Following gene: 51598587

Centisome position: 36.91

GC content: 31.11

Gene sequence:

>1572_bases
ATGAAATACATAAAAATAGCTTTAATACTAACTATCTTCTCTTTAATAGCCTGTACTAATAATGGAAAAAAAGAAAAAAT
AACCTTTAGAGCGTCAAACTTAAGCGAACCATCATCACTTGATCCACAGCTCTCAACAGACCTTTACGGTAGCAATATTA
TTACAAACCTATTCTTAGGGCTAGCAGTAAAAGATTCACAAACTGGAAGATATAAACCAGGACTTGCAAAAAGCTGGGAT
ATTTCTGGCAAGGGAACTATTTACACATTTACCCTAAGAGAAGATATAGTTTGGAGCGATGGAGTTGCCATTACTGCTGA
AGAGATAAAAAAATCATATCTAAGAATTTTAAATAAAAAAACAGCTGCACTATATGCCAATTTAGTAAAGTCTACAATAA
AAAACGCACAAGAATATTTTGATGAGACAGTGCCTGAATCTGAGCTTGGTATAAAAGCTATTGACAGTAAAACCCTGGAA
ATAACATTAACATCTCCAAAGCCTTATTTCCCCGATATGCTAACAAACTCAGCATACATACCAGTTCCAATACATATTGT
TGAAAAATATGGAGAAAATTGGACAAATCCCGAAAATATAGTTGTTAATGGCGCATACAAACTCAAAGAAAGATTAATTA
ACGATAAAATCGTAATAGAAAAAAATGAAAAATATTATAATGCAAAAAATGTAGAAATTGATGAAGTGATATTTTATCCA
ATAGAAGGTAATGTAGCTTACAATATGTACATAAACGGTGAACTCGATTTTCTGCAAGGAGCAGAAAAGAGCAATTTAGA
AGAAATTAAAATAAGAGATGATTATTACTCAGGATTAAAAAATGGAGTGGCATACATAGCATTCAATACAACCATAAAGC
CACTAGATAATTTAAAAGTTCGACAAGCTATCTCACTTGCTATTGATAGAGAAACTTTATGTAAAATAGTTTTAAAAGGA
AGTTCAGAGCCAACAAGAAACTTAACTCCAAAATTTGATGATTATTCTTATGGAAAAAATTTAATATTATTTGATCCCGA
GAATGCAAAAAAACTTTTAGCTGAAGCTGGATATCCAGATGGGAAAGGATTCCCTACATTAAAATATAAAGTCTCAGGGG
GAGGCCAAAAAGGAGCAGAATTCTTACAAGAACAATTTAAAAAAATCCTAAACATTAAAATAGAAATCGAAAACGAGGAA
TGGACAACATTTTTAGGAAGCAGAAGAACTGGAAATTACCAAATTTCAAGTGTGGGATGGATAGGAGACTATTTTGATCC
TTTAACATTCTTAGAAAGCTTATTTACAACAGAAAATCATTTTTTTGGAGCATACAAATATTCAAACAAAGAGTATGATG
CATTAATAACAAAATCTAATTTAGAACTTGATCCAATAAAAAGGCAAGACATTTTAAGAGAAGCTGAAGCAATCATAGTA
GAAAAAGATTTTCCTATGGCGCCCTTATCTATACCCAAATCTCATTACCTTTTCAGAAATGATAAATGGACAGGATGGGC
GCCAAATATTGCAGAAAGCTATTTATATGAAAATATTAAAACTAAAAAATAA

Upstream 100 bases:

>100_bases
CCCATATTTAAAAGCAAAATAAAGTAAATATTTTTTATTAAAAAAGATTAATAAAAAATCAACAAAACAAATATGTAAAA
TATTGAAAAAGGAAAATCCC

Downstream 100 bases:

>100_bases
TAAATTAAGTAATTTTAAAATTAAGATTTAAAATTAATATCTACGGAGGGGCAATTATAAAATTAAAGTTAACTTTAAAA
CGGAGGAATAAAAAATTATG

Product: oligopeptide ABC transporter, periplasmic oligopeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MKYIKIALILTIFSLIACTNNGKKEKITFRASNLSEPSSLDPQLSTDLYGSNIITNLFLGLAVKDSQTGRYKPGLAKSWD
ISGKGTIYTFTLREDIVWSDGVAITAEEIKKSYLRILNKKTAALYANLVKSTIKNAQEYFDETVPESELGIKAIDSKTLE
ITLTSPKPYFPDMLTNSAYIPVPIHIVEKYGENWTNPENIVVNGAYKLKERLINDKIVIEKNEKYYNAKNVEIDEVIFYP
IEGNVAYNMYINGELDFLQGAEKSNLEEIKIRDDYYSGLKNGVAYIAFNTTIKPLDNLKVRQAISLAIDRETLCKIVLKG
SSEPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPDGKGFPTLKYKVSGGGQKGAEFLQEQFKKILNIKIEIENEE
WTTFLGSRRTGNYQISSVGWIGDYFDPLTFLESLFTTENHFFGAYKYSNKEYDALITKSNLELDPIKRQDILREAEAIIV
EKDFPMAPLSIPKSHYLFRNDKWTGWAPNIAESYLYENIKTKK

Sequences:

>Translated_523_residues
MKYIKIALILTIFSLIACTNNGKKEKITFRASNLSEPSSLDPQLSTDLYGSNIITNLFLGLAVKDSQTGRYKPGLAKSWD
ISGKGTIYTFTLREDIVWSDGVAITAEEIKKSYLRILNKKTAALYANLVKSTIKNAQEYFDETVPESELGIKAIDSKTLE
ITLTSPKPYFPDMLTNSAYIPVPIHIVEKYGENWTNPENIVVNGAYKLKERLINDKIVIEKNEKYYNAKNVEIDEVIFYP
IEGNVAYNMYINGELDFLQGAEKSNLEEIKIRDDYYSGLKNGVAYIAFNTTIKPLDNLKVRQAISLAIDRETLCKIVLKG
SSEPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPDGKGFPTLKYKVSGGGQKGAEFLQEQFKKILNIKIEIENEE
WTTFLGSRRTGNYQISSVGWIGDYFDPLTFLESLFTTENHFFGAYKYSNKEYDALITKSNLELDPIKRQDILREAEAIIV
EKDFPMAPLSIPKSHYLFRNDKWTGWAPNIAESYLYENIKTKK
>Mature_523_residues
MKYIKIALILTIFSLIACTNNGKKEKITFRASNLSEPSSLDPQLSTDLYGSNIITNLFLGLAVKDSQTGRYKPGLAKSWD
ISGKGTIYTFTLREDIVWSDGVAITAEEIKKSYLRILNKKTAALYANLVKSTIKNAQEYFDETVPESELGIKAIDSKTLE
ITLTSPKPYFPDMLTNSAYIPVPIHIVEKYGENWTNPENIVVNGAYKLKERLINDKIVIEKNEKYYNAKNVEIDEVIFYP
IEGNVAYNMYINGELDFLQGAEKSNLEEIKIRDDYYSGLKNGVAYIAFNTTIKPLDNLKVRQAISLAIDRETLCKIVLKG
SSEPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPDGKGFPTLKYKVSGGGQKGAEFLQEQFKKILNIKIEIENEE
WTTFLGSRRTGNYQISSVGWIGDYFDPLTFLESLFTTENHFFGAYKYSNKEYDALITKSNLELDPIKRQDILREAEAIIV
EKDFPMAPLSIPKSHYLFRNDKWTGWAPNIAESYLYENIKTKK

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=477, Percent_Identity=33.5429769392034, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=33.8266384778013, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1789397, Length=477, Percent_Identity=32.9140461215933, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1789966, Length=516, Percent_Identity=25.3875968992248, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787052, Length=493, Percent_Identity=26.1663286004057, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789887, Length=470, Percent_Identity=24.8936170212766, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1787551, Length=449, Percent_Identity=22.71714922049, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59459; Mature: 59459

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIKIALILTIFSLIACTNNGKKEKITFRASNLSEPSSLDPQLSTDLYGSNIITNLFLG
CCEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHE
LAVKDSQTGRYKPGLAKSWDISGKGTIYTFTLREDIVWSDGVAITAEEIKKSYLRILNKK
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCEEEEHHHHHHHHHHHHCCH
TAALYANLVKSTIKNAQEYFDETVPESELGIKAIDSKTLEITLTSPKPYFPDMLTNSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHCCCCCC
PVPIHIVEKYGENWTNPENIVVNGAYKLKERLINDKIVIEKNEKYYNAKNVEIDEVIFYP
EEHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCEEEEECCCCEECCCCCEEEEEEEEE
IEGNVAYNMYINGELDFLQGAEKSNLEEIKIRDDYYSGLKNGVAYIAFNTTIKPLDNLKV
ECCCEEEEEEECCCCHHHCCCCCCCCEEEEECCHHHHHHHCCEEEEEEECEECCCCCCHH
RQAISLAIDRETLCKIVLKGSSEPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPD
HHHHHHHCCHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCC
GKGFPTLKYKVSGGGQKGAEFLQEQFKKILNIKIEIENEEWTTFLGSRRTGNYQISSVGW
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEEEEECCCEEEEECCCCCCCEEEEEECC
IGDYFDPLTFLESLFTTENHFFGAYKYSNKEYDALITKSNLELDPIKRQDILREAEAIIV
CHHHCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHCCEEEE
EKDFPMAPLSIPKSHYLFRNDKWTGWAPNIAESYLYENIKTKK
ECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKYIKIALILTIFSLIACTNNGKKEKITFRASNLSEPSSLDPQLSTDLYGSNIITNLFLG
CCEEHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHE
LAVKDSQTGRYKPGLAKSWDISGKGTIYTFTLREDIVWSDGVAITAEEIKKSYLRILNKK
EEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCEEEEHHHHHHHHHHHHCCH
TAALYANLVKSTIKNAQEYFDETVPESELGIKAIDSKTLEITLTSPKPYFPDMLTNSAYI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEECCCCCCCCHHHCCCCCC
PVPIHIVEKYGENWTNPENIVVNGAYKLKERLINDKIVIEKNEKYYNAKNVEIDEVIFYP
EEHHHHHHHHCCCCCCCCCEEEECHHHHHHHHCCCEEEEECCCCEECCCCCEEEEEEEEE
IEGNVAYNMYINGELDFLQGAEKSNLEEIKIRDDYYSGLKNGVAYIAFNTTIKPLDNLKV
ECCCEEEEEEECCCCHHHCCCCCCCCEEEEECCHHHHHHHCCEEEEEEECEECCCCCCHH
RQAISLAIDRETLCKIVLKGSSEPTRNLTPKFDDYSYGKNLILFDPENAKKLLAEAGYPD
HHHHHHHCCHHHHHHHEECCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCC
GKGFPTLKYKVSGGGQKGAEFLQEQFKKILNIKIEIENEEWTTFLGSRRTGNYQISSVGW
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEEEEECCCEEEEECCCCCCCEEEEEECC
IGDYFDPLTFLESLFTTENHFFGAYKYSNKEYDALITKSNLELDPIKRQDILREAEAIIV
CHHHCCHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHCCEEEE
EKDFPMAPLSIPKSHYLFRNDKWTGWAPNIAESYLYENIKTKK
ECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]