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The map label for this gene is glpF [H]

Identifier: 50843742

GI number: 50843742

Start: 2504189

End: 2504941

Strand: Reverse

Name: glpF [H]

Synonym: PPA2305

Alternate gene names: 50843742

Gene position: 2504941-2504189 (Counterclockwise)

Preceding gene: 50843744

Following gene: 50843741

Centisome position: 97.84

GC content: 60.96

Gene sequence:

>753_bases
ATGCTTCTCGAAACCGCGGTGCCCGGTGCAACGATCTTCGGGTCGGAGTTCTTGGGCACCTTGATCCTCATTCTCCTGGG
TTGTGGCGTCGTCGCCAATAACCTCCTTCCCAAGAGCAAGGGCCACGCTAACGCCCCCGGTTCCCTCCAGATTAACTGGG
GATGGGGCTTCGGCGTGATGTTCGGGGTTTACGCCGCTTACAAGACCGGTGGGCATCTGAATCCGGCTGTGACCGTCGGT
CTGGCCATCGCCGGCAAGGATCTGGCCCCTGGTATCCCTGCTACGGCTGGCAACATCACGATCTACATCCTCGCCCAGTT
CGCCGGTGCCTTTGTCGGTGCGGTGCTGTGCTGGCTGGCGTACAAACAGCACTATGACGAGGATTGCGACCCCGCCCTCA
AGCTCGGCACTTTCGCTACCGGTCCTGAGGTTCGCAATCCGCTGTGGAACACCATAACTGAGGCCATCGGCACCTGGGTC
CTTGTGCTGTGGGTCATCCTCTCCGGCTTCACCCAGGGAGTGAAAATTGGCCCCCTGGCTGTCGGTTTGCTCATTGTCTC
CATCGGTGCCTCCCTCGGTGGCCCCACCGGATATGCCATCAACCCGGCCCGTGACCTCTCTCCCCGTATCGCCCACGCCG
TTTTGCCAATCAAGGGCAAGGGTGACAGCGATTGGGGATATGCCTGGGTCCCGGTCGTTGGCCCGATGATCGGTGCTGTC
CTGGCCGCTCTCACCTACTCCATTTTCTGGTGA

Upstream 100 bases:

>100_bases
GGGGGCGGGACGACGGTCCCCGTTCCTAAATTCTACGGGATCGACTGCCACCGGCGAGTAAACCGGTGTCAACGGATGAC
ACCACGACGAAGGAGTCTTC

Downstream 100 bases:

>100_bases
GTGGGAATACCTCTCGTGACACACTGATTGCGCGTCAGCCGTAATGTGTCAGACACGTCAATGCAGATAAGAAAGGCAAC
CAACATGAGCGATGAGAAGT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLLETAVPGATIFGSEFLGTLILILLGCGVVANNLLPKSKGHANAPGSLQINWGWGFGVMFGVYAAYKTGGHLNPAVTVG
LAIAGKDLAPGIPATAGNITIYILAQFAGAFVGAVLCWLAYKQHYDEDCDPALKLGTFATGPEVRNPLWNTITEAIGTWV
LVLWVILSGFTQGVKIGPLAVGLLIVSIGASLGGPTGYAINPARDLSPRIAHAVLPIKGKGDSDWGYAWVPVVGPMIGAV
LAALTYSIFW

Sequences:

>Translated_250_residues
MLLETAVPGATIFGSEFLGTLILILLGCGVVANNLLPKSKGHANAPGSLQINWGWGFGVMFGVYAAYKTGGHLNPAVTVG
LAIAGKDLAPGIPATAGNITIYILAQFAGAFVGAVLCWLAYKQHYDEDCDPALKLGTFATGPEVRNPLWNTITEAIGTWV
LVLWVILSGFTQGVKIGPLAVGLLIVSIGASLGGPTGYAINPARDLSPRIAHAVLPIKGKGDSDWGYAWVPVVGPMIGAV
LAALTYSIFW
>Mature_250_residues
MLLETAVPGATIFGSEFLGTLILILLGCGVVANNLLPKSKGHANAPGSLQINWGWGFGVMFGVYAAYKTGGHLNPAVTVG
LAIAGKDLAPGIPATAGNITIYILAQFAGAFVGAVLCWLAYKQHYDEDCDPALKLGTFATGPEVRNPLWNTITEAIGTWV
LVLWVILSGFTQGVKIGPLAVGLLIVSIGASLGGPTGYAINPARDLSPRIAHAVLPIKGKGDSDWGYAWVPVVGPMIGAV
LAALTYSIFW

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=268, Percent_Identity=35.0746268656716, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI4826645, Length=256, Percent_Identity=33.59375, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI4502187, Length=254, Percent_Identity=33.8582677165354, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI22538420, Length=253, Percent_Identity=31.2252964426877, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1790362, Length=259, Percent_Identity=36.6795366795367, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71994009, Length=249, Percent_Identity=32.9317269076305, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17531429, Length=239, Percent_Identity=32.2175732217573, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531431, Length=239, Percent_Identity=32.2175732217573, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17533613, Length=247, Percent_Identity=32.3886639676113, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32564052, Length=247, Percent_Identity=32.3886639676113, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71992966, Length=251, Percent_Identity=32.2709163346614, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17544068, Length=257, Percent_Identity=31.5175097276265, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558372, Length=249, Percent_Identity=28.9156626506024, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71993722, Length=237, Percent_Identity=28.2700421940928, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=213, Percent_Identity=36.150234741784, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25983; Mature: 25983

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLETAVPGATIFGSEFLGTLILILLGCGVVANNLLPKSKGHANAPGSLQINWGWGFGVM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHH
FGVYAAYKTGGHLNPAVTVGLAIAGKDLAPGIPATAGNITIYILAQFAGAFVGAVLCWLA
HHHHHHHHCCCCCCHHHEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
YKQHYDEDCDPALKLGTFATGPEVRNPLWNTITEAIGTWVLVLWVILSGFTQGVKIGPLA
HHHHCCCCCCHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
VGLLIVSIGASLGGPTGYAINPARDLSPRIAHAVLPIKGKGDSDWGYAWVPVVGPMIGAV
HHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHEEEEECCCCCCCCCEEHHHHHHHHHHHH
LAALTYSIFW
HHHHHHHHCC
>Mature Secondary Structure
MLLETAVPGATIFGSEFLGTLILILLGCGVVANNLLPKSKGHANAPGSLQINWGWGFGVM
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHH
FGVYAAYKTGGHLNPAVTVGLAIAGKDLAPGIPATAGNITIYILAQFAGAFVGAVLCWLA
HHHHHHHHCCCCCCHHHEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHH
YKQHYDEDCDPALKLGTFATGPEVRNPLWNTITEAIGTWVLVLWVILSGFTQGVKIGPLA
HHHHCCCCCCHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHH
VGLLIVSIGASLGGPTGYAINPARDLSPRIAHAVLPIKGKGDSDWGYAWVPVVGPMIGAV
HHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHEEEEECCCCCCCCCEEHHHHHHHHHHHH
LAALTYSIFW
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7565084; 11463916 [H]