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The map label for this gene is purD [H]

Identifier: 50843440

GI number: 50843440

Start: 2165117

End: 2166436

Strand: Reverse

Name: purD [H]

Synonym: PPA1993

Alternate gene names: 50843440

Gene position: 2166436-2165117 (Counterclockwise)

Preceding gene: 50843441

Following gene: 50843439

Centisome position: 84.62

GC content: 64.17

Gene sequence:

>1320_bases
ATGACTGTTCTCGTCCTTGGGTCCGGTGGCCGCGAGCATGCCCTTGCCTTGGCCTGCCATCGTGACCCAGAGGTTGCCGC
CGTCCACGTCGCTCCCGGTAACCCGGGGACGGCATCCTTCGCCACGAATCATCCGTTGGATCCGTGCGATTGTGATGCCG
TTGTCTCCTTGGCGCGTGGCCTGGAAGCTGACCTCGTCGTCGTCGGCCCGGAGGCGCCGCTGGTTGCCGGGGTGGCCGAT
GCTCTGCGCGAGTCCGGGATCGACTGCTTCGGTCCAAGTGCCGAGGCCGCCCAGTTGGAAGGGTCGAAGGCTTTCGCCAA
GGAAGTGATGGCTGCTGCCCGTGTGCCGACGGCAGCCTCCCGTTCTTGCGTCGATGCCAATTCCGTCTCGGCTGCCCTTG
ACGAGTTCGGGCCACCATACGTGGTCAAGGACGATGGTCTGGCCGGGGGCAAGGGAGTCGTCGTCACACACGATCGCGCC
GAGGCTCTCGCTCATGCCAAACGGTGTTTCGAGTCCGATCACCGGGTGGTCGTTGAGGAGTACCTTGACGGCCCGGAGGT
AAGTCTTTTTGCTATCTGTGATGGCACCCGTGCCCTTCCGATGCAGCCGGCCCAGGACTTTAAGCGGGTCGGGAATGGGG
GAGTTGGTCCGAACACCGGTGGCATGGGTGCCTACACCCCGTTGCCGTGGGCCCCAGACGATCTCGTCGAGACGGTGTCG
ACTACCATCATCTCGCCAACCCTGAGGGCCATGGCGGATCGCGGTACCCCATTCGTCGGCCTGCTTTACGTGGGGTTGGC
GCTCACTTCTGCTGGTCCCAAAGTCGTTGAGTTCAACGTGCGATTCGGCGATCCCGAGACTGAGCCACTACTGAGTATGG
TGGAGTCACCTATGGCCCAGATCATGGCTGCCGCGGCTCGTGGAGACCTGTCCGGTGTTGACGGTCTTCGCTTCCGTTGT
GGCGCAGCCGTCGGCGTCGTGATGGCCAGCGAGGGTTATCCCGCTTGCCCGGCGAAAGGGCGCCGGGTCGTCGCCCCGGT
TGACGACGAGGACGTTTTGCACGCTGGGACTCGTATCGAAGGGGACGGCCTCGTTACCAGTGGTGGCCGCGTTCTCGTCG
TGCTCGGCCACGGCGAAACTCTGGCCGGAGCCCGCGCTGACGCCTACCGCAGGATCGACCAGTTGGAGGTTAGTGACGGG
TTCCACCGCACGGACATTGCCCGCCATGCAGCCGAGCTCGAGGCCGTTGACGAAATGGCTGACGGACGCGCACCGCAGTC
CGAGAATTCCACCAACCTGACCGACGGGGAGTCTTCATGA

Upstream 100 bases:

>100_bases
GGACGCGACGAGTCAGTCGTGATCAACGACCTCATTGACTGATTCCTCCCGGCTGACGGGAATAGACGCTGTAGGTTCTA
GGCTAGTCCCCGTGTCCAGC

Downstream 100 bases:

>100_bases
GCGTACCCAATATCCTTGCCACCCGTTATGCCTCCGCGGCCATGCGTTCCATCTGGACGCCGGAAAACAAAATCCGTGCC
GAGCGCAGACTCTGGCTGAC

Product: phosphoribosylamine-glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTVLVLGSGGREHALALACHRDPEVAAVHVAPGNPGTASFATNHPLDPCDCDAVVSLARGLEADLVVVGPEAPLVAGVAD
ALRESGIDCFGPSAEAAQLEGSKAFAKEVMAAARVPTAASRSCVDANSVSAALDEFGPPYVVKDDGLAGGKGVVVTHDRA
EALAHAKRCFESDHRVVVEEYLDGPEVSLFAICDGTRALPMQPAQDFKRVGNGGVGPNTGGMGAYTPLPWAPDDLVETVS
TTIISPTLRAMADRGTPFVGLLYVGLALTSAGPKVVEFNVRFGDPETEPLLSMVESPMAQIMAAAARGDLSGVDGLRFRC
GAAVGVVMASEGYPACPAKGRRVVAPVDDEDVLHAGTRIEGDGLVTSGGRVLVVLGHGETLAGARADAYRRIDQLEVSDG
FHRTDIARHAAELEAVDEMADGRAPQSENSTNLTDGESS

Sequences:

>Translated_439_residues
MTVLVLGSGGREHALALACHRDPEVAAVHVAPGNPGTASFATNHPLDPCDCDAVVSLARGLEADLVVVGPEAPLVAGVAD
ALRESGIDCFGPSAEAAQLEGSKAFAKEVMAAARVPTAASRSCVDANSVSAALDEFGPPYVVKDDGLAGGKGVVVTHDRA
EALAHAKRCFESDHRVVVEEYLDGPEVSLFAICDGTRALPMQPAQDFKRVGNGGVGPNTGGMGAYTPLPWAPDDLVETVS
TTIISPTLRAMADRGTPFVGLLYVGLALTSAGPKVVEFNVRFGDPETEPLLSMVESPMAQIMAAAARGDLSGVDGLRFRC
GAAVGVVMASEGYPACPAKGRRVVAPVDDEDVLHAGTRIEGDGLVTSGGRVLVVLGHGETLAGARADAYRRIDQLEVSDG
FHRTDIARHAAELEAVDEMADGRAPQSENSTNLTDGESS
>Mature_438_residues
TVLVLGSGGREHALALACHRDPEVAAVHVAPGNPGTASFATNHPLDPCDCDAVVSLARGLEADLVVVGPEAPLVAGVADA
LRESGIDCFGPSAEAAQLEGSKAFAKEVMAAARVPTAASRSCVDANSVSAALDEFGPPYVVKDDGLAGGKGVVVTHDRAE
ALAHAKRCFESDHRVVVEEYLDGPEVSLFAICDGTRALPMQPAQDFKRVGNGGVGPNTGGMGAYTPLPWAPDDLVETVST
TIISPTLRAMADRGTPFVGLLYVGLALTSAGPKVVEFNVRFGDPETEPLLSMVESPMAQIMAAAARGDLSGVDGLRFRCG
AAVGVVMASEGYPACPAKGRRVVAPVDDEDVLHAGTRIEGDGLVTSGGRVLVVLGHGETLAGARADAYRRIDQLEVSDGF
HRTDIARHAAELEAVDEMADGRAPQSENSTNLTDGESS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=421, Percent_Identity=40.1425178147268, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI209869995, Length=421, Percent_Identity=40.1425178147268, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI209869993, Length=421, Percent_Identity=40.1425178147268, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI28416899, Length=421, Percent_Identity=40.1425178147268, Blast_Score=290, Evalue=1e-78,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=44.392523364486, Blast_Score=316, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=35.4609929078014, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321203, Length=444, Percent_Identity=36.9369369369369, Blast_Score=265, Evalue=1e-71,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=38.5150812064965, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=38.5150812064965, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45308; Mature: 45177

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLVLGSGGREHALALACHRDPEVAAVHVAPGNPGTASFATNHPLDPCDCDAVVSLARG
CEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
LEADLVVVGPEAPLVAGVADALRESGIDCFGPSAEAAQLEGSKAFAKEVMAAARVPTAAS
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCC
RSCVDANSVSAALDEFGPPYVVKDDGLAGGKGVVVTHDRAEALAHAKRCFESDHRVVVEE
CCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEHHH
YLDGPEVSLFAICDGTRALPMQPAQDFKRVGNGGVGPNTGGMGAYTPLPWAPDDLVETVS
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TTIISPTLRAMADRGTPFVGLLYVGLALTSAGPKVVEFNVRFGDPETEPLLSMVESPMAQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
IMAAAARGDLSGVDGLRFRCGAAVGVVMASEGYPACPAKGRRVVAPVDDEDVLHAGTRIE
HHHHHHCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCEEEECCCCCHHHHCCCEEC
GDGLVTSGGRVLVVLGHGETLAGARADAYRRIDQLEVSDGFHRTDIARHAAELEAVDEMA
CCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
DGRAPQSENSTNLTDGESS
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVLVLGSGGREHALALACHRDPEVAAVHVAPGNPGTASFATNHPLDPCDCDAVVSLARG
EEEEECCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
LEADLVVVGPEAPLVAGVADALRESGIDCFGPSAEAAQLEGSKAFAKEVMAAARVPTAAS
CCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCC
RSCVDANSVSAALDEFGPPYVVKDDGLAGGKGVVVTHDRAEALAHAKRCFESDHRVVVEE
CCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCCCEEEHHH
YLDGPEVSLFAICDGTRALPMQPAQDFKRVGNGGVGPNTGGMGAYTPLPWAPDDLVETVS
HCCCCCEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TTIISPTLRAMADRGTPFVGLLYVGLALTSAGPKVVEFNVRFGDPETEPLLSMVESPMAQ
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
IMAAAARGDLSGVDGLRFRCGAAVGVVMASEGYPACPAKGRRVVAPVDDEDVLHAGTRIE
HHHHHHCCCCCCCCCCCEECCCEEEEEEECCCCCCCCCCCCEEEECCCCCHHHHCCCEEC
GDGLVTSGGRVLVVLGHGETLAGARADAYRRIDQLEVSDGFHRTDIARHAAELEAVDEMA
CCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
DGRAPQSENSTNLTDGESS
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]