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The map label for this gene is purA

Identifier: 50843441

GI number: 50843441

Start: 2166495

End: 2167781

Strand: Reverse

Name: purA

Synonym: PPA1994

Alternate gene names: 50843441

Gene position: 2167781-2166495 (Counterclockwise)

Preceding gene: 50843442

Following gene: 50843440

Centisome position: 84.67

GC content: 59.83

Gene sequence:

>1287_bases
ATGCCTGGAATCGTCGTCATGGGTACCCAATGGGGTGACGAGGGCAAGGGCAAGGCCACCGATCTCATGTCCGAACGCGT
CGACTATTGCGTGCGGTATTCGGGAGGCAATAACGCTGGACACACTGTCGTCGTCGGTGACGAGAAGTTCTTCATGCACC
TGCTTCCGTCTGGGGTCCTTAACCCGAACTCCACGGCCGTGCTCGGCAACGGAGTCGTGCTCAACCTTGACGTGCTGGCC
GATGAGATCGACGCCTTGCGCGGCCGCGGCGTAGACATTCCGCACCCGCTCATCAGCGCCAATGCGCACCTCATCACCCC
GTACCATCAGACTCTCGACAAGGTCACTGAGCGTTTTCTTGGCAGTCGACGTATCGGCACCACGGGTCGTGGCATCGGGC
CGACCTACTCCGACAAGATCAATCGGATGGGCATTCGCGTCCAGGATCTTTTCGACGAATCGATCCTGCGTCAGAAGGTC
GAGGCCTCTCTAGACCAAAAGAACCAGATCCTGGTGAAGATCTACAACCGTCGCGCTATCGATCCAGGCGAGGTTGCCGA
CGGTCTGCTGGCCCACGCCGAGCGGATCCGCCCCTACGTCGTTGACGTGGCTCGAGTTCTCAACAAGGGCCTTGACGAGG
GCAAGGTCGTACTTTTTGAGGGCGCCCAGGCCCATCATCTTGACGTTGACTTCGGCACGTATCCGTATGTCACCTCGTCC
AACCCGATCGCTGCGGGCGCGTGTACTGGCTCAGGCGTCGGACCGACTCGCATCGACCGCATTGTTGGCATCGCTAAGGC
GTACACCACTCGCGTTGGTGAAGGTCCGTTCCCGACCGAGCTCTGTGACGATGATGGTGAGCGGCTGCGCAGTGAAGGTG
GTGAGTACGGTGTCACGACTAAGCGTCCGCGTCGCTGTGGCTGGTTTGACGCGCTGGTGGTGCAGCAGGCGGTCATGATC
AATTCGGTGACTGACCTGTTCCTCACCAAGCTTGACGTGCTGACAGGCTGGGAGAGGATCCCGGTCTGCGTGGGCTACGA
CATTGACGGTGAGCGTACCGACATTATGCCCGTCACCCAGTCCGATTTGCACCACGCCAAGCCGGTCTATGAGTTCCTGG
ATGGCTGGAGTGAAGACATTTCTACGGCACGTTCCTTCGATGATCTGCCGCGCAACTGTCAGGCTTACGTGCGTCGTCTA
GAGGAGCTTGTGGGTACTCGGGTCTCCGGGATCGGCGTGGGGGCCGGACGCGACGAGTCAGTCGTGATCAACGACCTCAT
TGACTGA

Upstream 100 bases:

>100_bases
TTGTCGTTATGGGCACGTAAAGTGTCCTTGGTTGCACAGCGGCATGTGCCGATGCAACCAGAGGCAACCCGCACAGTGGT
GCGAGGAAATGGAGGAACAC

Downstream 100 bases:

>100_bases
TTCCTCCCGGCTGACGGGAATAGACGCTGTAGGTTCTAGGCTAGTCCCCGTGTCCAGCATGACTGTTCTCGTCCTTGGGT
CCGGTGGCCGCGAGCATGCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MPGIVVMGTQWGDEGKGKATDLMSERVDYCVRYSGGNNAGHTVVVGDEKFFMHLLPSGVLNPNSTAVLGNGVVLNLDVLA
DEIDALRGRGVDIPHPLISANAHLITPYHQTLDKVTERFLGSRRIGTTGRGIGPTYSDKINRMGIRVQDLFDESILRQKV
EASLDQKNQILVKIYNRRAIDPGEVADGLLAHAERIRPYVVDVARVLNKGLDEGKVVLFEGAQAHHLDVDFGTYPYVTSS
NPIAAGACTGSGVGPTRIDRIVGIAKAYTTRVGEGPFPTELCDDDGERLRSEGGEYGVTTKRPRRCGWFDALVVQQAVMI
NSVTDLFLTKLDVLTGWERIPVCVGYDIDGERTDIMPVTQSDLHHAKPVYEFLDGWSEDISTARSFDDLPRNCQAYVRRL
EELVGTRVSGIGVGAGRDESVVINDLID

Sequences:

>Translated_428_residues
MPGIVVMGTQWGDEGKGKATDLMSERVDYCVRYSGGNNAGHTVVVGDEKFFMHLLPSGVLNPNSTAVLGNGVVLNLDVLA
DEIDALRGRGVDIPHPLISANAHLITPYHQTLDKVTERFLGSRRIGTTGRGIGPTYSDKINRMGIRVQDLFDESILRQKV
EASLDQKNQILVKIYNRRAIDPGEVADGLLAHAERIRPYVVDVARVLNKGLDEGKVVLFEGAQAHHLDVDFGTYPYVTSS
NPIAAGACTGSGVGPTRIDRIVGIAKAYTTRVGEGPFPTELCDDDGERLRSEGGEYGVTTKRPRRCGWFDALVVQQAVMI
NSVTDLFLTKLDVLTGWERIPVCVGYDIDGERTDIMPVTQSDLHHAKPVYEFLDGWSEDISTARSFDDLPRNCQAYVRRL
EELVGTRVSGIGVGAGRDESVVINDLID
>Mature_427_residues
PGIVVMGTQWGDEGKGKATDLMSERVDYCVRYSGGNNAGHTVVVGDEKFFMHLLPSGVLNPNSTAVLGNGVVLNLDVLAD
EIDALRGRGVDIPHPLISANAHLITPYHQTLDKVTERFLGSRRIGTTGRGIGPTYSDKINRMGIRVQDLFDESILRQKVE
ASLDQKNQILVKIYNRRAIDPGEVADGLLAHAERIRPYVVDVARVLNKGLDEGKVVLFEGAQAHHLDVDFGTYPYVTSSN
PIAAGACTGSGVGPTRIDRIVGIAKAYTTRVGEGPFPTELCDDDGERLRSEGGEYGVTTKRPRRCGWFDALVVQQAVMIN
SVTDLFLTKLDVLTGWERIPVCVGYDIDGERTDIMPVTQSDLHHAKPVYEFLDGWSEDISTARSFDDLPRNCQAYVRRLE
ELVGTRVSGIGVGAGRDESVVINDLID

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=44.8598130841121, Blast_Score=328, Evalue=6e-90,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.1924882629108, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=42.2110552763819, Blast_Score=286, Evalue=3e-77,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=46.3700234192037, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=42.2632794457275, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=42.2632794457275, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324109, Length=425, Percent_Identity=42.5882352941177, Blast_Score=346, Evalue=3e-96,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=43.6018957345972, Blast_Score=332, Evalue=4e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PROAC (Q6A6A3)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056668.1
- ProteinModelPortal:   Q6A6A3
- SMR:   Q6A6A3
- GeneID:   2931301
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1994
- NMPDR:   fig|267747.1.peg.1945
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   PACN267747:PPA1994-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46776; Mature: 46644

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGIVVMGTQWGDEGKGKATDLMSERVDYCVRYSGGNNAGHTVVVGDEKFFMHLLPSGVL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEECCHHHHHHHCCCCCC
NPNSTAVLGNGVVLNLDVLADEIDALRGRGVDIPHPLISANAHLITPYHQTLDKVTERFL
CCCCCEEEECCEEEEHHHHHHHHHHHHCCCCCCCCHHHCCCCEEECHHHHHHHHHHHHHH
GSRRIGTTGRGIGPTYSDKINRMGIRVQDLFDESILRQKVEASLDQKNQILVKIYNRRAI
CCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
DPGEVADGLLAHAERIRPYVVDVARVLNKGLDEGKVVLFEGAQAHHLDVDFGTYPYVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCEEECC
NPIAAGACTGSGVGPTRIDRIVGIAKAYTTRVGEGPFPTELCDDDGERLRSEGGEYGVTT
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHCCCCCCCCC
KRPRRCGWFDALVVQQAVMINSVTDLFLTKLDVLTGWERIPVCVGYDIDGERTDIMPVTQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEECCCH
SDLHHAKPVYEFLDGWSEDISTARSFDDLPRNCQAYVRRLEELVGTRVSGIGVGAGRDES
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCC
VVINDLID
EEHHCCCC
>Mature Secondary Structure 
PGIVVMGTQWGDEGKGKATDLMSERVDYCVRYSGGNNAGHTVVVGDEKFFMHLLPSGVL
CCEEEEECCCCCCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEECCHHHHHHHCCCCCC
NPNSTAVLGNGVVLNLDVLADEIDALRGRGVDIPHPLISANAHLITPYHQTLDKVTERFL
CCCCCEEEECCEEEEHHHHHHHHHHHHCCCCCCCCHHHCCCCEEECHHHHHHHHHHHHHH
GSRRIGTTGRGIGPTYSDKINRMGIRVQDLFDESILRQKVEASLDQKNQILVKIYNRRAI
CCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCC
DPGEVADGLLAHAERIRPYVVDVARVLNKGLDEGKVVLFEGAQAHHLDVDFGTYPYVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCCCCEEECC
NPIAAGACTGSGVGPTRIDRIVGIAKAYTTRVGEGPFPTELCDDDGERLRSEGGEYGVTT
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHCCCCCCCCC
KRPRRCGWFDALVVQQAVMINSVTDLFLTKLDVLTGWERIPVCVGYDIDGERTDIMPVTQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEECCCH
SDLHHAKPVYEFLDGWSEDISTARSFDDLPRNCQAYVRRLEELVGTRVSGIGVGAGRDES
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCC
VVINDLID
EEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA