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The map label for this gene is purB [H]

Identifier: 50843439

GI number: 50843439

Start: 2163687

End: 2165120

Strand: Reverse

Name: purB [H]

Synonym: PPA1992

Alternate gene names: 50843439

Gene position: 2165120-2163687 (Counterclockwise)

Preceding gene: 50843440

Following gene: 50843435

Centisome position: 84.57

GC content: 59.48

Gene sequence:

>1434_bases
ATGAGCGTACCCAATATCCTTGCCACCCGTTATGCCTCCGCGGCCATGCGTTCCATCTGGACGCCGGAAAACAAAATCCG
TGCCGAGCGCAGACTCTGGCTGACCGTCATGGAAGCTCAAGCCGATCTCGGTGTGAGCTTTGGTGGCGACGATCCCGAAC
AGGTGTTGGCCGACTACCAGGCGGTTCTCGACGACGTTGACCTTGACTCGATCGCCGAGCGGGAGGCGATTACTCGTCAT
GACGTCAAGGCCAGGATTGAGGAGTTCAACGCCCTGGCCGGTCATGAGCACATTCACAAGGGCATGACGAGCCGTGACCT
CACCGAAAACATTGAGCAGATGCAGGTGCTGGAGTCGCTGCGGCTAGTGCGCGACAAAATGGTTGCCACGCTCAACCAGC
TGTCGCGCCTGGCCGCTCAGTTCTCCGATCAAGCCATTGCTGGACGCTCTCACAATGTTGCCGCCCAGGTGACGACCCTG
GGCAAGCGGTTCGCCACCGCTATCGATGAGATGCTGGTGGCATGGGATCACGTCAACGAGCTCATTGAGCGCTACCCGGC
TCGTGGTATCAAGGGCCCGATGGGAACTGCCCAGGACATGCTTGACCTGCTTGGCGGTGATGAGTCCAAGCTTGACGATC
TTGAGCATCGCGTTGCTGATCATCTGGGGTTTAACCAGGTACTCACTTCGACCGGTCAGATTTACCCGAGGTCCTTGGAC
TTTGAGGTCATGTCAACTCTGGTGCAGGTGCTGTCAGGCCCGTCTAACCTGGCGACGTCTATCCGCCTTATGGCCGGTAA
CGAACTTGTCACCGAGGGGTTCAAGCCCGGCCAGGTGGGTTCCTCGGCGATGCCGCACAAGATGAATGCTCGTTCCTGCG
AGCGGGTCAACGGCTTCCTTGTTGTGTTACGTGGCTATTTGACTATGGCCACCGGTCTGGCGGGAGTGCAGTGGAACGAG
GGTGACGTGTCCTGTTCGGTGGTGCGTCGTGTGGCGCTGCCCGATTCCTTCTATGCCATTGATGGTGCTTTTGAGACCTT
TTTGACGATCCTTGGCGACTTCGGGGCGTTCCCCGCTGTCATTGAGGCTGAGCTTGAGCGCTACCTGCCCTTCCTGACGA
CTACTAAGGTCCTTATGGCTTGTGTGCGTGCTGGCGTCGGTCGGGAGGACGCCCACGAGGCCATCAAGGAACACGCCGTA
GCCGTGGCCCTCGACATGCGCGAAACCGGTCGCACTGGTAACGACCTTTTCGCCCGTCTGGCCGCCGATCCGCGCATCCC
GCTCACCAAGGAGCAGCTACGTGGTTTGGTGTCCTCTCCGCTGGATCTCACTGGTGGCGCCCAGGATCAGGTCGAGGCGC
TGTGCGCCAAGGTGGAGGACATTGTTCGGGAATACCCGAAGGCTGCCAACTACCTGCCTGGTCTGGTGTTGTGA

Upstream 100 bases:

>100_bases
TTGCCCGCCATGCAGCCGAGCTCGAGGCCGTTGACGAAATGGCTGACGGACGCGCACCGCAGTCCGAGAATTCCACCAAC
CTGACCGACGGGGAGTCTTC

Downstream 100 bases:

>100_bases
GGTGATCGTGCCGGGGCACGCACCACGTTGTCATCACATCCCGTGAGAGCACAACGATGCTCCGGCAACGCCGAGAGGCT
CGACGACGTCACCTGGTAGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSVPNILATRYASAAMRSIWTPENKIRAERRLWLTVMEAQADLGVSFGGDDPEQVLADYQAVLDDVDLDSIAEREAITRH
DVKARIEEFNALAGHEHIHKGMTSRDLTENIEQMQVLESLRLVRDKMVATLNQLSRLAAQFSDQAIAGRSHNVAAQVTTL
GKRFATAIDEMLVAWDHVNELIERYPARGIKGPMGTAQDMLDLLGGDESKLDDLEHRVADHLGFNQVLTSTGQIYPRSLD
FEVMSTLVQVLSGPSNLATSIRLMAGNELVTEGFKPGQVGSSAMPHKMNARSCERVNGFLVVLRGYLTMATGLAGVQWNE
GDVSCSVVRRVALPDSFYAIDGAFETFLTILGDFGAFPAVIEAELERYLPFLTTTKVLMACVRAGVGREDAHEAIKEHAV
AVALDMRETGRTGNDLFARLAADPRIPLTKEQLRGLVSSPLDLTGGAQDQVEALCAKVEDIVREYPKAANYLPGLVL

Sequences:

>Translated_477_residues
MSVPNILATRYASAAMRSIWTPENKIRAERRLWLTVMEAQADLGVSFGGDDPEQVLADYQAVLDDVDLDSIAEREAITRH
DVKARIEEFNALAGHEHIHKGMTSRDLTENIEQMQVLESLRLVRDKMVATLNQLSRLAAQFSDQAIAGRSHNVAAQVTTL
GKRFATAIDEMLVAWDHVNELIERYPARGIKGPMGTAQDMLDLLGGDESKLDDLEHRVADHLGFNQVLTSTGQIYPRSLD
FEVMSTLVQVLSGPSNLATSIRLMAGNELVTEGFKPGQVGSSAMPHKMNARSCERVNGFLVVLRGYLTMATGLAGVQWNE
GDVSCSVVRRVALPDSFYAIDGAFETFLTILGDFGAFPAVIEAELERYLPFLTTTKVLMACVRAGVGREDAHEAIKEHAV
AVALDMRETGRTGNDLFARLAADPRIPLTKEQLRGLVSSPLDLTGGAQDQVEALCAKVEDIVREYPKAANYLPGLVL
>Mature_476_residues
SVPNILATRYASAAMRSIWTPENKIRAERRLWLTVMEAQADLGVSFGGDDPEQVLADYQAVLDDVDLDSIAEREAITRHD
VKARIEEFNALAGHEHIHKGMTSRDLTENIEQMQVLESLRLVRDKMVATLNQLSRLAAQFSDQAIAGRSHNVAAQVTTLG
KRFATAIDEMLVAWDHVNELIERYPARGIKGPMGTAQDMLDLLGGDESKLDDLEHRVADHLGFNQVLTSTGQIYPRSLDF
EVMSTLVQVLSGPSNLATSIRLMAGNELVTEGFKPGQVGSSAMPHKMNARSCERVNGFLVVLRGYLTMATGLAGVQWNEG
DVSCSVVRRVALPDSFYAIDGAFETFLTILGDFGAFPAVIEAELERYLPFLTTTKVLMACVRAGVGREDAHEAIKEHAVA
VALDMRETGRTGNDLFARLAADPRIPLTKEQLRGLVSSPLDLTGGAQDQVEALCAKVEDIVREYPKAANYLPGLVL

Specific function: Unknown

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=448, Percent_Identity=31.25, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=31.1688311688312, Blast_Score=193, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17508577, Length=456, Percent_Identity=27.1929824561404, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32564234, Length=334, Percent_Identity=28.4431137724551, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6323391, Length=473, Percent_Identity=33.615221987315, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI24647570, Length=465, Percent_Identity=30.752688172043, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52231; Mature: 52100

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPNILATRYASAAMRSIWTPENKIRAERRLWLTVMEAQADLGVSFGGDDPEQVLADYQ
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AVLDDVDLDSIAEREAITRHDVKARIEEFNALAGHEHIHKGMTSRDLTENIEQMQVLESL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RLVRDKMVATLNQLSRLAAQFSDQAIAGRSHNVAAQVTTLGKRFATAIDEMLVAWDHVNE
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIERYPARGIKGPMGTAQDMLDLLGGDESKLDDLEHRVADHLGFNQVLTSTGQIYPRSLD
HHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCC
FEVMSTLVQVLSGPSNLATSIRLMAGNELVTEGFKPGQVGSSAMPHKMNARSCERVNGFL
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VVLRGYLTMATGLAGVQWNEGDVSCSVVRRVALPDSFYAIDGAFETFLTILGDFGAFPAV
HHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHH
IEAELERYLPFLTTTKVLMACVRAGVGREDAHEAIKEHAVAVALDMRETGRTGNDLFARL
HHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEHHHCCCCHHHHHHHH
AADPRIPLTKEQLRGLVSSPLDLTGGAQDQVEALCAKVEDIVREYPKAANYLPGLVL
HCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure 
SVPNILATRYASAAMRSIWTPENKIRAERRLWLTVMEAQADLGVSFGGDDPEQVLADYQ
CCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
AVLDDVDLDSIAEREAITRHDVKARIEEFNALAGHEHIHKGMTSRDLTENIEQMQVLESL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RLVRDKMVATLNQLSRLAAQFSDQAIAGRSHNVAAQVTTLGKRFATAIDEMLVAWDHVNE
HHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIERYPARGIKGPMGTAQDMLDLLGGDESKLDDLEHRVADHLGFNQVLTSTGQIYPRSLD
HHHHCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCC
FEVMSTLVQVLSGPSNLATSIRLMAGNELVTEGFKPGQVGSSAMPHKMNARSCERVNGFL
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VVLRGYLTMATGLAGVQWNEGDVSCSVVRRVALPDSFYAIDGAFETFLTILGDFGAFPAV
HHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHH
IEAELERYLPFLTTTKVLMACVRAGVGREDAHEAIKEHAVAVALDMRETGRTGNDLFARL
HHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEHHHCCCCHHHHHHHH
AADPRIPLTKEQLRGLVSSPLDLTGGAQDQVEALCAKVEDIVREYPKAANYLPGLVL
HCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]