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The map label for this gene is ahpF [H]

Identifier: 50843437

GI number: 50843437

Start: 2160996

End: 2162702

Strand: Direct

Name: ahpF [H]

Synonym: PPA1990

Alternate gene names: 50843437

Gene position: 2160996-2162702 (Clockwise)

Preceding gene: 50843436

Following gene: 50843438

Centisome position: 84.41

GC content: 63.56

Gene sequence:

>1707_bases
ATGAGACCCTCGCCCCGTCGATCGACCTCGTCGGCAAGATCTGAGTTCCGACCCGAGCATCTGCTGCAGGGGCGCTCGTC
CCTGCGGCGGATCCCCGGACCGGTACCCTTCCCTCCCCCCTTCAGGAGAACCATGCTTGATAATGCCCTGACCACTCAGC
TCAAGACCTACCTCGAGATGGTGCGCGAGCCGATCGTCCTGGTGCCTTCCCCATACCGGGGTGACGACGCCGCAAAATCG
TCAAAGTCGGCTCACATGGACGAGCTTTTATCCGAGATCGCCGCCTTGTCTGACAAAGTGAGCGTCGGTAAGCCCGTCGA
CAATGAGCGCACCCCCTCCTTTGCCATCACCCGCCCCGGAAGCCCCATCGACATCCGCTTTGCGGGCCTGCCGATGGGCC
ACGAATTCACCTCCCTCGTACTGGCCCTGCTGCAGGTAGGTGGACACCCCGTCAAGGAGGAGCAGTCCCTCATCGACGCC
GTGCGCGCGGTCAAGGGTGAGCACCACTTCGTCACCTACATGTCACTAACCTGCCAAAATTGCCCGACGGTAGTGCAGGC
CCTCAACGCAATGGCCGTCCTCAACCCGAATATTCACCACACCGCCGTTGACGGTGGCACCCACACCGATGAGGTAGAAG
CCAAAGGAATCGCCTCGGTGCCGGCCATCTACCTGGACGGCGAGGACTGGGGCTCCGGGCGCATGGACATGGCCGAAATC
CTCGAGCGTCTCGACGCCGACGCCGCCCAGGGAGCCAAGGAAGACCTCTCGCAGCGCGACCCCTACGACGTGCTCGTCGT
AGGGGCGGGTCCCGCCGGTGCCGCGGCCGCCGTGTACGCGGCTCGTAAGGGCATTCGCACCGCCATGGTCGGGTCTCGAA
TCGGCGGCCAGGTACTCGATACCGAGGCCATCGACAACCTCATCTCGGTGCCGCACACCACCGGTCCGCGTCTGGCCGAC
GCCCTCCGCAGCCACGTCAACGACTACAACATTGACGTTATTGAGCGTCAGACCGCCAGCGCCATAGAGACCACCGGCGG
TATGACCACCGTGCATCTGACCGACGGCGACCTGCGGGCGCGCTCAGTCATCGTGGCCACCGGTGCCCGCTGGCGCAACC
TTGGCGTGCCTGGCGAGAAGGAATACCGCACCAAGGGTGTGACCTACTGCCCGCACTGCGATGGCCCGCTATTCACAGGC
AAAAAGGTGGCCGTCGTCGGAGGTGGAAACTCCGGCATTGAGGCCGCTATCGACATTGCCGGCGTCGTCGACCACGTCAC
CGTCGTGGAGTTCCTCGACGCCCTCAAGGCTGACGACGTACTACTACGCACCCTGAACTCACTGCCGAACGTCGACGTCA
TCACCTCGGCCCGCACCACCCAGATCCTCGGCGACGGATCCAAGGTGACCGGTCTGGAATACGAGGACCGCACCAGCGGC
GACGTTAAGACCATCGAGCTAGCAGGTGTGTTTGTCCAAATCGGTCTGCTGCCCAACACCGAATGGCTCGAAGGTGCCCT
GGAGCGCAACCAGCGGGGAGAAATCGTCATCGACGAGCGTGGCACCACCTCAGCCCCCGGAATCTTCGCCGCCGGTGACT
GCACCACTCAGCCGTACAAGCAGATCATCATCTCGATGGGGGCCGGCGCCACCGCTGCTCTAGGCGCCTTCGACCACCTC
ATCCGTCAAAATCCGACACAGAACTGA

Upstream 100 bases:

>100_bases
ATTGGGCGTAATGCCACCGAGCTGGTCCGCAAGGTTAAGGCCGCCCAATACATCTACACCCACCCAGGCGAGGTGTGCCC
CGCCAAGTGGGAAGAAGGCG

Downstream 100 bases:

>100_bases
GGCCGTGTGAACCTTCATGATCTGCGGTATTTCGTCGCCCTGGCCGAAGAACACCACTTCGGCCGGGCTGCGGCGTCATG
CGGTGTCAGCCAGCCCACCC

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: Alkyl hydroperoxide reductase F52A protein [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MRPSPRRSTSSARSEFRPEHLLQGRSSLRRIPGPVPFPPPFRRTMLDNALTTQLKTYLEMVREPIVLVPSPYRGDDAAKS
SKSAHMDELLSEIAALSDKVSVGKPVDNERTPSFAITRPGSPIDIRFAGLPMGHEFTSLVLALLQVGGHPVKEEQSLIDA
VRAVKGEHHFVTYMSLTCQNCPTVVQALNAMAVLNPNIHHTAVDGGTHTDEVEAKGIASVPAIYLDGEDWGSGRMDMAEI
LERLDADAAQGAKEDLSQRDPYDVLVVGAGPAGAAAAVYAARKGIRTAMVGSRIGGQVLDTEAIDNLISVPHTTGPRLAD
ALRSHVNDYNIDVIERQTASAIETTGGMTTVHLTDGDLRARSVIVATGARWRNLGVPGEKEYRTKGVTYCPHCDGPLFTG
KKVAVVGGGNSGIEAAIDIAGVVDHVTVVEFLDALKADDVLLRTLNSLPNVDVITSARTTQILGDGSKVTGLEYEDRTSG
DVKTIELAGVFVQIGLLPNTEWLEGALERNQRGEIVIDERGTTSAPGIFAAGDCTTQPYKQIIISMGAGATAALGAFDHL
IRQNPTQN

Sequences:

>Translated_568_residues
MRPSPRRSTSSARSEFRPEHLLQGRSSLRRIPGPVPFPPPFRRTMLDNALTTQLKTYLEMVREPIVLVPSPYRGDDAAKS
SKSAHMDELLSEIAALSDKVSVGKPVDNERTPSFAITRPGSPIDIRFAGLPMGHEFTSLVLALLQVGGHPVKEEQSLIDA
VRAVKGEHHFVTYMSLTCQNCPTVVQALNAMAVLNPNIHHTAVDGGTHTDEVEAKGIASVPAIYLDGEDWGSGRMDMAEI
LERLDADAAQGAKEDLSQRDPYDVLVVGAGPAGAAAAVYAARKGIRTAMVGSRIGGQVLDTEAIDNLISVPHTTGPRLAD
ALRSHVNDYNIDVIERQTASAIETTGGMTTVHLTDGDLRARSVIVATGARWRNLGVPGEKEYRTKGVTYCPHCDGPLFTG
KKVAVVGGGNSGIEAAIDIAGVVDHVTVVEFLDALKADDVLLRTLNSLPNVDVITSARTTQILGDGSKVTGLEYEDRTSG
DVKTIELAGVFVQIGLLPNTEWLEGALERNQRGEIVIDERGTTSAPGIFAAGDCTTQPYKQIIISMGAGATAALGAFDHL
IRQNPTQN
>Mature_568_residues
MRPSPRRSTSSARSEFRPEHLLQGRSSLRRIPGPVPFPPPFRRTMLDNALTTQLKTYLEMVREPIVLVPSPYRGDDAAKS
SKSAHMDELLSEIAALSDKVSVGKPVDNERTPSFAITRPGSPIDIRFAGLPMGHEFTSLVLALLQVGGHPVKEEQSLIDA
VRAVKGEHHFVTYMSLTCQNCPTVVQALNAMAVLNPNIHHTAVDGGTHTDEVEAKGIASVPAIYLDGEDWGSGRMDMAEI
LERLDADAAQGAKEDLSQRDPYDVLVVGAGPAGAAAAVYAARKGIRTAMVGSRIGGQVLDTEAIDNLISVPHTTGPRLAD
ALRSHVNDYNIDVIERQTASAIETTGGMTTVHLTDGDLRARSVIVATGARWRNLGVPGEKEYRTKGVTYCPHCDGPLFTG
KKVAVVGGGNSGIEAAIDIAGVVDHVTVVEFLDALKADDVLLRTLNSLPNVDVITSARTTQILGDGSKVTGLEYEDRTSG
DVKTIELAGVFVQIGLLPNTEWLEGALERNQRGEIVIDERGTTSAPGIFAAGDCTTQPYKQIIISMGAGATAALGAFDHL
IRQNPTQN

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=525, Percent_Identity=59.4285714285714, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1787114, Length=308, Percent_Identity=33.4415584415584, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321898, Length=326, Percent_Identity=29.4478527607362, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=29.5819935691318, Blast_Score=134, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 60677; Mature: 60677

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSPRRSTSSARSEFRPEHLLQGRSSLRRIPGPVPFPPPFRRTMLDNALTTQLKTYLEM
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VREPIVLVPSPYRGDDAAKSSKSAHMDELLSEIAALSDKVSVGKPVDNERTPSFAITRPG
HHCCEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
SPIDIRFAGLPMGHEFTSLVLALLQVGGHPVKEEQSLIDAVRAVKGEHHFVTYMSLTCQN
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCC
CPTVVQALNAMAVLNPNIHHTAVDGGTHTDEVEAKGIASVPAIYLDGEDWGSGRMDMAEI
CHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
LERLDADAAQGAKEDLSQRDPYDVLVVGAGPAGAAAAVYAARKGIRTAMVGSRIGGQVLD
HHHHCCHHHCCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEC
TEAIDNLISVPHTTGPRLADALRSHVNDYNIDVIERQTASAIETTGGMTTVHLTDGDLRA
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCEEEEEEECCCCCC
RSVIVATGARWRNLGVPGEKEYRTKGVTYCPHCDGPLFTGKKVAVVGGGNSGIEAAIDIA
CEEEEECCCCCCCCCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
GVVDHVTVVEFLDALKADDVLLRTLNSLPNVDVITSARTTQILGDGSKVTGLEYEDRTSG
HHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCEEEECCCCEEECCCCCCCCCC
DVKTIELAGVFVQIGLLPNTEWLEGALERNQRGEIVIDERGTTSAPGIFAAGDCTTQPYK
CEEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHH
QIIISMGAGATAALGAFDHLIRQNPTQN
HHHHHCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRPSPRRSTSSARSEFRPEHLLQGRSSLRRIPGPVPFPPPFRRTMLDNALTTQLKTYLEM
CCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VREPIVLVPSPYRGDDAAKSSKSAHMDELLSEIAALSDKVSVGKPVDNERTPSFAITRPG
HHCCEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCC
SPIDIRFAGLPMGHEFTSLVLALLQVGGHPVKEEQSLIDAVRAVKGEHHFVTYMSLTCQN
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCC
CPTVVQALNAMAVLNPNIHHTAVDGGTHTDEVEAKGIASVPAIYLDGEDWGSGRMDMAEI
CHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHH
LERLDADAAQGAKEDLSQRDPYDVLVVGAGPAGAAAAVYAARKGIRTAMVGSRIGGQVLD
HHHHCCHHHCCCHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHCCCCEEC
TEAIDNLISVPHTTGPRLADALRSHVNDYNIDVIERQTASAIETTGGMTTVHLTDGDLRA
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCEEEEEEECCCCCC
RSVIVATGARWRNLGVPGEKEYRTKGVTYCPHCDGPLFTGKKVAVVGGGNSGIEAAIDIA
CEEEEECCCCCCCCCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHH
GVVDHVTVVEFLDALKADDVLLRTLNSLPNVDVITSARTTQILGDGSKVTGLEYEDRTSG
HHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCEEEECCCCEEECCCCCCCCCC
DVKTIELAGVFVQIGLLPNTEWLEGALERNQRGEIVIDERGTTSAPGIFAAGDCTTQPYK
CEEEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCEEECCCCCCCHHH
QIIISMGAGATAALGAFDHLIRQNPTQN
HHHHHCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 1592833 [H]