Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 50843382

GI number: 50843382

Start: 2093854

End: 2094639

Strand: Reverse

Name: nuoE [H]

Synonym: PPA1932

Alternate gene names: 50843382

Gene position: 2094639-2093854 (Counterclockwise)

Preceding gene: 50843383

Following gene: 50843381

Centisome position: 81.81

GC content: 60.56

Gene sequence:

>786_bases
ATGAGCGATCGCGAACCCGTCAACCTGGGATACCCCTACGTCGAGTCTTTCCACTCGGACTTCTCGGGGACCGGCGGAGT
CGATCAGACCGACCGTTCTACCAATATCGACGAGCACACCATCGAGGAGATGCATCAGATCGCCTCGCGTTACCCCGACT
CCCGTTCGGCGTTGCTGCCGATCCTGCACCTGGTTCAGTCGGTGGACGGACGCATCTCGCCGGTCGGCATTGAGACTGCG
GCTGAAGTGCTCGGCATTACCACCGCCCAGGTATCCGGGGTGGCGACCTTCTACACCATGTATAAGAAGCACCCTGCGGG
CCAGCATCACATCGGTGTCTGCACCACGGCGCTGTGCGCCGTCATGGGTGGCGAGGAGGTGCTTGCCCGTGTCGAGAAGA
AGCTTGGCATTAAAGAGGGGCAGACAACCCCCGACGGCAAGTTCTCTCTTGAGCGGGTGGAGTGTAATGCTGCCTGCGAT
TTCGCGCCGATCATGATGGTCAACTGGGAATACATGGATAACATGACTCCGATCCGCGCTGAGGAAATCTTGGATTCCTT
GGCTCGCGACGAAGAGGTTCATTCCACCCGCGGTGCGAAGATCACTAGCTGGCGTGAGGCTGAGCGAGTGCTCGCTGGCT
TCCCCGATGGTCGGGCTGACGAGGGCCCTAGCGCCGGTGAGGCATCTCTGCAGGGTGTCCGGCTAGCTAAGCAGAGCGGC
TGGAAGGCTCCAGATCCGAATAACCTGCCCGCTCCCGCTGAGCCCGTGGAAGAGGAGAAGAAGTGA

Upstream 100 bases:

>100_bases
TCAACGTGCAGTCCCTGCCCGCCATGTGTGAGGGCGGAATGCTTTCTGACGTGGTGCCGTCTCTGGCATCCTTGGACCCC
GTCATGGGAGGAGTCGACCG

Downstream 100 bases:

>100_bases
CCGATCCACTGACCCCGGTTCTGTCGGCCAATTGGGATGAAGAGCGCAGTTGGAAGCTGCTTAACTACGAGCGACAGGGC
GGATACACCGGCCTTCGTAA

Product: NADH dehydrogenase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MSDREPVNLGYPYVESFHSDFSGTGGVDQTDRSTNIDEHTIEEMHQIASRYPDSRSALLPILHLVQSVDGRISPVGIETA
AEVLGITTAQVSGVATFYTMYKKHPAGQHHIGVCTTALCAVMGGEEVLARVEKKLGIKEGQTTPDGKFSLERVECNAACD
FAPIMMVNWEYMDNMTPIRAEEILDSLARDEEVHSTRGAKITSWREAERVLAGFPDGRADEGPSAGEASLQGVRLAKQSG
WKAPDPNNLPAPAEPVEEEKK

Sequences:

>Translated_261_residues
MSDREPVNLGYPYVESFHSDFSGTGGVDQTDRSTNIDEHTIEEMHQIASRYPDSRSALLPILHLVQSVDGRISPVGIETA
AEVLGITTAQVSGVATFYTMYKKHPAGQHHIGVCTTALCAVMGGEEVLARVEKKLGIKEGQTTPDGKFSLERVECNAACD
FAPIMMVNWEYMDNMTPIRAEEILDSLARDEEVHSTRGAKITSWREAERVLAGFPDGRADEGPSAGEASLQGVRLAKQSG
WKAPDPNNLPAPAEPVEEEKK
>Mature_260_residues
SDREPVNLGYPYVESFHSDFSGTGGVDQTDRSTNIDEHTIEEMHQIASRYPDSRSALLPILHLVQSVDGRISPVGIETAA
EVLGITTAQVSGVATFYTMYKKHPAGQHHIGVCTTALCAVMGGEEVLARVEKKLGIKEGQTTPDGKFSLERVECNAACDF
APIMMVNWEYMDNMTPIRAEEILDSLARDEEVHSTRGAKITSWREAERVLAGFPDGRADEGPSAGEASLQGVRLAKQSGW
KAPDPNNLPAPAEPVEEEKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=162, Percent_Identity=38.2716049382716, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1788621, Length=143, Percent_Identity=39.1608391608392, Blast_Score=100, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17561328, Length=148, Percent_Identity=38.5135135135135, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI18859877, Length=152, Percent_Identity=37.5, Blast_Score=114, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21355353, Length=143, Percent_Identity=33.5664335664336, Blast_Score=94, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 28438; Mature: 28307

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDREPVNLGYPYVESFHSDFSGTGGVDQTDRSTNIDEHTIEEMHQIASRYPDSRSALLP
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
ILHLVQSVDGRISPVGIETAAEVLGITTAQVSGVATFYTMYKKHPAGQHHIGVCTTALCA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VMGGEEVLARVEKKLGIKEGQTTPDGKFSLERVECNAACDFAPIMMVNWEYMDNMTPIRA
HHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHCCCCCCCH
EEILDSLARDEEVHSTRGAKITSWREAERVLAGFPDGRADEGPSAGEASLQGVRLAKQSG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
WKAPDPNNLPAPAEPVEEEKK
CCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDREPVNLGYPYVESFHSDFSGTGGVDQTDRSTNIDEHTIEEMHQIASRYPDSRSALLP
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHH
ILHLVQSVDGRISPVGIETAAEVLGITTAQVSGVATFYTMYKKHPAGQHHIGVCTTALCA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VMGGEEVLARVEKKLGIKEGQTTPDGKFSLERVECNAACDFAPIMMVNWEYMDNMTPIRA
HHCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEHHHHCCCCCCCH
EEILDSLARDEEVHSTRGAKITSWREAERVLAGFPDGRADEGPSAGEASLQGVRLAKQSG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCC
WKAPDPNNLPAPAEPVEEEKK
CCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]