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The map label for this gene is gltX

Identifier: 50843323

GI number: 50843323

Start: 2021881

End: 2023374

Strand: Reverse

Name: gltX

Synonym: PPA1869

Alternate gene names: 50843323

Gene position: 2023374-2021881 (Counterclockwise)

Preceding gene: 50843327

Following gene: 50843322

Centisome position: 79.03

GC content: 57.83

Gene sequence:

>1494_bases
ATGACTGATTTGCATCCCGCGCGCACGCGCGTTGCCCCCTCGCCCACTGGTGATCCTCACGTCGGTACGGCCTATATGGC
CTTGTTCGACAAGGCATGGGCACAACGCACCGGTGGCCAGTTTGTGCTGCGCATCGAGGACACCGATCGCACTCGTCTGG
TGGCGGATTCGGAAGATCAGATTTATCAGACACTCGAGTGGCTGGGGTTATCTCCTGACGAGTCCCCGCTCGTCGGTGGC
CCGTATGAGCCGTACAAGCAGTCTGAGCGGTTAGACACGTACAAACCGCTCGTTGAGAAGCTCATCGAGTCCGGTCACGC
CTATCGTTGCTGGTGCTCCCAGGAGAGGCTCAAGCAGTTGCGTGAGGAGCGTGCTGCAGCGAAGTCGCCGGAGACTGGTT
ACGACCGGATGTGTCTGGGGATGTCGAAAGAGGAAAGAGCTAAGCTGCCAGGTTTCACCGAGACCCCGGTGGTTCGGATG
CTTATTCCTGAGGATGTCGATCTGGAGTTTGATGACCTTATCCGGGGCACGGTGCGCGCTCCTCGTCCTGACGATCAGGT
GATCCTCAAAGCTGACGGGTTCCCGACCTATCACATGGCCGTCGTCGTCGACGATCATCTGATGGGTATCGATACCGTCG
TGCGCGGTGAGGAATGGATTTCGTCAACGCCCAAGCATCTATTGCTTTACAAGTGGCTGGGATGGGAAGCGCCGAAGTTC
GCTCATATGCCCCTGCTGCGTAATACTGATAAATCCAAAATTTCTAAGCGTAAGAACCCGGCTGCTCGGCTGATGTGGTT
CCGTGAGCAAGGCTATCTGCCTGAGGCGTTGCGTAATTTCCTTCAATTGCTGGCTTACCCGACGGTCGCTGAGGGCCAGG
AAATGGAGGACTTTGATTCCTTCGTATCCCGGTTCGATTGGGGGAACGTTTCTACAGGCGGCCCGGTCTTCGACGTCACT
AAATTGGACTGGCTCAACGGTCAGTACATTCGCAGCCTTGACGCTGAGGTCCTCACCGATCGTGTGCTCGACTACGCCGA
CCAGATGTGTCAGGCGCGACACGTTCTGGGTCGTGATCTGACCCAGGAGGATCGCTGTGTCATCCACGCCGCGATGCCGC
TAGTTCGGGAAAGATTAACGTTGTTGTCCGAGGCCCTGCCCAAGCTGGCCTTCCTGCTGACTGACGACGACGCCTTGGTC
TTCGATGAGGATTCCGTGGCCAAGCTCAAGGCGGGAGCCGACGACATCCAGCATGCCGCTGTTGAGGTACTCAAGGGACT
TGACGATTTTTCCGCTGACGCTATCCAAGCTGCCTTGCGCGCCCGACTGTGCGACGAGATGGGCATCAAGCCCCGCCTAG
CCTTTGCGCCGGTTCGGGTGGCTGTGACTGGTTCGCGGGTGTCCCCGCCGCTGTTCGAGGCGATGGAGATTCTGGGCAAG
GAGTCGACTCTTCGTCGTATGCAAAGGTTCGAGGCTGAGATAGACCAGAAGTAA

Upstream 100 bases:

>100_bases
ATGAAGTCCGAGCTGGACTCAGCCGGGTTTCCAGTAGGTTCTGCCATGGCGGGAACTTTACCGGAGTCTGTCAGCGGATC
CCGATAGACTTCGTGTCATC

Downstream 100 bases:

>100_bases
TGACTGAACACAGGGAGACGAGGGTTGCGCTGCGGCGCCATCCACTTCTGCGACGCCCAATAGTGTGGTTCTGGGCGCTC
GTCACCTGTTATTGGGCGTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTDLHPARTRVAPSPTGDPHVGTAYMALFDKAWAQRTGGQFVLRIEDTDRTRLVADSEDQIYQTLEWLGLSPDESPLVGG
PYEPYKQSERLDTYKPLVEKLIESGHAYRCWCSQERLKQLREERAAAKSPETGYDRMCLGMSKEERAKLPGFTETPVVRM
LIPEDVDLEFDDLIRGTVRAPRPDDQVILKADGFPTYHMAVVVDDHLMGIDTVVRGEEWISSTPKHLLLYKWLGWEAPKF
AHMPLLRNTDKSKISKRKNPAARLMWFREQGYLPEALRNFLQLLAYPTVAEGQEMEDFDSFVSRFDWGNVSTGGPVFDVT
KLDWLNGQYIRSLDAEVLTDRVLDYADQMCQARHVLGRDLTQEDRCVIHAAMPLVRERLTLLSEALPKLAFLLTDDDALV
FDEDSVAKLKAGADDIQHAAVEVLKGLDDFSADAIQAALRARLCDEMGIKPRLAFAPVRVAVTGSRVSPPLFEAMEILGK
ESTLRRMQRFEAEIDQK

Sequences:

>Translated_497_residues
MTDLHPARTRVAPSPTGDPHVGTAYMALFDKAWAQRTGGQFVLRIEDTDRTRLVADSEDQIYQTLEWLGLSPDESPLVGG
PYEPYKQSERLDTYKPLVEKLIESGHAYRCWCSQERLKQLREERAAAKSPETGYDRMCLGMSKEERAKLPGFTETPVVRM
LIPEDVDLEFDDLIRGTVRAPRPDDQVILKADGFPTYHMAVVVDDHLMGIDTVVRGEEWISSTPKHLLLYKWLGWEAPKF
AHMPLLRNTDKSKISKRKNPAARLMWFREQGYLPEALRNFLQLLAYPTVAEGQEMEDFDSFVSRFDWGNVSTGGPVFDVT
KLDWLNGQYIRSLDAEVLTDRVLDYADQMCQARHVLGRDLTQEDRCVIHAAMPLVRERLTLLSEALPKLAFLLTDDDALV
FDEDSVAKLKAGADDIQHAAVEVLKGLDDFSADAIQAALRARLCDEMGIKPRLAFAPVRVAVTGSRVSPPLFEAMEILGK
ESTLRRMQRFEAEIDQK
>Mature_496_residues
TDLHPARTRVAPSPTGDPHVGTAYMALFDKAWAQRTGGQFVLRIEDTDRTRLVADSEDQIYQTLEWLGLSPDESPLVGGP
YEPYKQSERLDTYKPLVEKLIESGHAYRCWCSQERLKQLREERAAAKSPETGYDRMCLGMSKEERAKLPGFTETPVVRML
IPEDVDLEFDDLIRGTVRAPRPDDQVILKADGFPTYHMAVVVDDHLMGIDTVVRGEEWISSTPKHLLLYKWLGWEAPKFA
HMPLLRNTDKSKISKRKNPAARLMWFREQGYLPEALRNFLQLLAYPTVAEGQEMEDFDSFVSRFDWGNVSTGGPVFDVTK
LDWLNGQYIRSLDAEVLTDRVLDYADQMCQARHVLGRDLTQEDRCVIHAAMPLVRERLTLLSEALPKLAFLLTDDDALVF
DEDSVAKLKAGADDIQHAAVEVLKGLDDFSADAIQAALRARLCDEMGIKPRLAFAPVRVAVTGSRVSPPLFEAMEILGKE
STLRRMQRFEAEIDQK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=36.5111561866126, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1788743, Length=486, Percent_Identity=34.156378600823, Blast_Score=242, Evalue=5e-65,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=31.1284046692607, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=32.7272727272727, Blast_Score=259, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324540, Length=508, Percent_Identity=33.2677165354331, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=34.7560975609756, Blast_Score=261, Evalue=8e-70,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PROAC (Q6A6M1)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056550.1
- ProteinModelPortal:   Q6A6M1
- SMR:   Q6A6M1
- GeneID:   2933042
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1869
- NMPDR:   fig|267747.1.peg.1827
- HOGENOM:   HBG628189
- OMA:   ICREKVN
- ProtClustDB:   PRK01406
- BioCyc:   PACN267747:PPA1869-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56312; Mature: 56181

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS01094 UPF0076

Important sites: BINDING 256-256

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLHPARTRVAPSPTGDPHVGTAYMALFDKAWAQRTGGQFVLRIEDTDRTRLVADSEDQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCHHH
IYQTLEWLGLSPDESPLVGGPYEPYKQSERLDTYKPLVEKLIESGHAYRCWCSQERLKQL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
REERAAAKSPETGYDRMCLGMSKEERAKLPGFTETPVVRMLIPEDVDLEFDDLIRGTVRA
HHHHHHCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCC
PRPDDQVILKADGFPTYHMAVVVDDHLMGIDTVVRGEEWISSTPKHLLLYKWLGWEAPKF
CCCCCCEEEEECCCCCEEEEEEECCCHHCHHHHHCCHHHHHCCCHHEEEEHHCCCCCCCH
AHMPLLRNTDKSKISKRKNPAARLMWFREQGYLPEALRNFLQLLAYPTVAEGQEMEDFDS
HCCCHHCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
FVSRFDWGNVSTGGPVFDVTKLDWLNGQYIRSLDAEVLTDRVLDYADQMCQARHVLGRDL
HHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TQEDRCVIHAAMPLVRERLTLLSEALPKLAFLLTDDDALVFDEDSVAKLKAGADDIQHAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCHHHHHCCHHHHHHHH
VEVLKGLDDFSADAIQAALRARLCDEMGIKPRLAFAPVRVAVTGSRVSPPLFEAMEILGK
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCHHHHHHHHHCC
ESTLRRMQRFEAEIDQK
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDLHPARTRVAPSPTGDPHVGTAYMALFDKAWAQRTGGQFVLRIEDTDRTRLVADSEDQ
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCHHH
IYQTLEWLGLSPDESPLVGGPYEPYKQSERLDTYKPLVEKLIESGHAYRCWCSQERLKQL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
REERAAAKSPETGYDRMCLGMSKEERAKLPGFTETPVVRMLIPEDVDLEFDDLIRGTVRA
HHHHHHCCCCCCCCHHHHCCCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCCC
PRPDDQVILKADGFPTYHMAVVVDDHLMGIDTVVRGEEWISSTPKHLLLYKWLGWEAPKF
CCCCCCEEEEECCCCCEEEEEEECCCHHCHHHHHCCHHHHHCCCHHEEEEHHCCCCCCCH
AHMPLLRNTDKSKISKRKNPAARLMWFREQGYLPEALRNFLQLLAYPTVAEGQEMEDFDS
HCCCHHCCCCHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
FVSRFDWGNVSTGGPVFDVTKLDWLNGQYIRSLDAEVLTDRVLDYADQMCQARHVLGRDL
HHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TQEDRCVIHAAMPLVRERLTLLSEALPKLAFLLTDDDALVFDEDSVAKLKAGADDIQHAA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEECCCCHHHHHCCHHHHHHHH
VEVLKGLDDFSADAIQAALRARLCDEMGIKPRLAFAPVRVAVTGSRVSPPLFEAMEILGK
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCHHHHHHHHHCC
ESTLRRMQRFEAEIDQK
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA