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The map label for this gene is tuf

Identifier: 50843327

GI number: 50843327

Start: 2029170

End: 2030363

Strand: Reverse

Name: tuf

Synonym: PPA1873

Alternate gene names: 50843327

Gene position: 2030363-2029170 (Counterclockwise)

Preceding gene: 50843328

Following gene: 50843323

Centisome position: 79.3

GC content: 61.73

Gene sequence:

>1194_bases
GTGGCAAAGGCCAAGTTCGAGCGGACCAAGCCGCACTGCAACATCGGCACCATCGGACACATCGACCACGGCAAGACGAC
CCTCACCGCGGCGATCTCCAAGGTGCTGCACGACAAGTACCCGGAGCTGAACGAGGAGTCGCCGTTCGACCAGATCGACA
AGGCTCCCGAGGAGCGTCAGCGCGGCATCACCATTTCGATCGCTCACATCGAGTACCAGACCGAGAAGCGTCACTACGCT
CACGTCGACTGCCCTGGGCACGCTGACTACGTCAAGAACATGATCACCGGTGCTGCTCAGATGGACGGCGCCATCCTCGT
GGTTGCTGCTACCGACGGCCCGATGCCTCAGACTCGCGAGCACGTTCTGCTCGCTCGTCAGGTGGGCGTGCCCGCCATCG
TCGTCGCCCTCAACAAGTGCGACATGGTTGACGATGAGGAGCTCATTGAGCTCGTCGAGATGGAGGTCCGCGAGCTGCTG
ACCTCGCAGGAGTTCGACGGCGACAACTGCCCTGTCGTTCGCATCTCCGCCTTCCAGGCCCTCCAGGGTGATGAGAAGTG
GACCCAGTCGATCCTCGACCTCATGGACGCCGTGGACGAGTACATCCCGCAGCCTGAGCGCGATCTCGACAAGCCCTTCC
TTATGCCGATCGAGGACGTCTTCACCATCACCGGCCGTGGCACCGTTGTCACCGGTCGTGTCGAGCGCGGCGTCGTCAAG
ACTGGCGAAGAGGTCGAGATCGTCGGTATCCACGAGAAGACCCAGAAGACCACCGTTACCGGTGTCGAGATGTTCCGCAA
GATCCTCGACGAGGGCCGCGCTGGTGAGAACGTCGGCGTTCTGCTCCGTGGCACCAAGAAGGAGGATGTCGTTCGCGGCA
TGGTCCTCTCCAAGCCTGGTTCCACCACCCCCCACACCGACTTCGAGGGCCAGGTCTACGTCCTCAAGAAGGATGAGGGT
GGCCGCCACAAGCCGTTCTTCTCCCACTACAGCCCCCAGTTCTACTTCCGTACCACGGACGTGACTGGCACTGTTGAGCT
CCCCGAGGGCACCGAGATGGTCATGCCTGGCGACAACACCGACATGACTGTGCACCTGATTCACCCGGTTGCCATGGAGG
ATCAGCTCAAGTTCGCTATCCGTGAGGGCGGCCGCACCGTCGGTGCTGGTCGAGTCACCAAGATCATCAAGTGA

Upstream 100 bases:

>100_bases
CTGGTCGGGGGCCCGTCCAGAAACGTTCCTGGGAACCTCCCAGGAATATCCACATGACGCGTCCCGTGCACAAGCGGGCG
ACATTCCAGAAGGAGCCCCA

Downstream 100 bases:

>100_bases
TGTGATCTGAGCTTTTCACGAGGCCCGTTCCCCTAGGGGAACGGGCCTCGTCGCATGTGAATACAGATAAATTCTGCTTA
CTTGGCAATAGGCAGCAAAG

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKAKFERTKPHCNIGTIGHIDHGKTTLTAAISKVLHDKYPELNEESPFDQIDKAPEERQRGITISIAHIEYQTEKRHYA
HVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPAIVVALNKCDMVDDEELIELVEMEVRELL
TSQEFDGDNCPVVRISAFQALQGDEKWTQSILDLMDAVDEYIPQPERDLDKPFLMPIEDVFTITGRGTVVTGRVERGVVK
TGEEVEIVGIHEKTQKTTVTGVEMFRKILDEGRAGENVGVLLRGTKKEDVVRGMVLSKPGSTTPHTDFEGQVYVLKKDEG
GRHKPFFSHYSPQFYFRTTDVTGTVELPEGTEMVMPGDNTDMTVHLIHPVAMEDQLKFAIREGGRTVGAGRVTKIIK

Sequences:

>Translated_397_residues
MAKAKFERTKPHCNIGTIGHIDHGKTTLTAAISKVLHDKYPELNEESPFDQIDKAPEERQRGITISIAHIEYQTEKRHYA
HVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPAIVVALNKCDMVDDEELIELVEMEVRELL
TSQEFDGDNCPVVRISAFQALQGDEKWTQSILDLMDAVDEYIPQPERDLDKPFLMPIEDVFTITGRGTVVTGRVERGVVK
TGEEVEIVGIHEKTQKTTVTGVEMFRKILDEGRAGENVGVLLRGTKKEDVVRGMVLSKPGSTTPHTDFEGQVYVLKKDEG
GRHKPFFSHYSPQFYFRTTDVTGTVELPEGTEMVMPGDNTDMTVHLIHPVAMEDQLKFAIREGGRTVGAGRVTKIIK
>Mature_396_residues
AKAKFERTKPHCNIGTIGHIDHGKTTLTAAISKVLHDKYPELNEESPFDQIDKAPEERQRGITISIAHIEYQTEKRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPAIVVALNKCDMVDDEELIELVEMEVRELLT
SQEFDGDNCPVVRISAFQALQGDEKWTQSILDLMDAVDEYIPQPERDLDKPFLMPIEDVFTITGRGTVVTGRVERGVVKT
GEEVEIVGIHEKTQKTTVTGVEMFRKILDEGRAGENVGVLLRGTKKEDVVRGMVLSKPGSTTPHTDFEGQVYVLKKDEGG
RHKPFFSHYSPQFYFRTTDVTGTVELPEGTEMVMPGDNTDMTVHLIHPVAMEDQLKFAIREGGRTVGAGRVTKIIK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=401, Percent_Identity=54.6134663341646, Blast_Score=450, Evalue=1e-126,
Organism=Homo sapiens, GI4503471, Length=349, Percent_Identity=34.0974212034384, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4503475, Length=349, Percent_Identity=33.2378223495702, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=29.0249433106576, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=28.7981859410431, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI194018522, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI194097354, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI194018520, Length=339, Percent_Identity=28.9085545722714, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=30.3030303030303, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI54607086, Length=325, Percent_Identity=27.6923076923077, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI4503507, Length=373, Percent_Identity=26.5415549597855, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157426893, Length=255, Percent_Identity=28.6274509803922, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI53729339, Length=232, Percent_Identity=31.4655172413793, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI53729337, Length=232, Percent_Identity=31.4655172413793, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=73.7373737373737, Blast_Score=581, Evalue=1e-167,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=73.7373737373737, Blast_Score=580, Evalue=1e-167,
Organism=Escherichia coli, GI2367247, Length=340, Percent_Identity=32.0588235294118, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI48994988, Length=259, Percent_Identity=32.8185328185328, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789108, Length=325, Percent_Identity=29.5384615384615, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788922, Length=243, Percent_Identity=27.9835390946502, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=49.0049751243781, Blast_Score=371, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=45.4545454545455, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=329, Percent_Identity=34.9544072948328, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=329, Percent_Identity=34.9544072948328, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=314, Percent_Identity=32.1656050955414, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=31.7518248175182, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=27.8801843317972, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=27.8801843317972, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.1061946902655, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=145, Percent_Identity=41.3793103448276, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=298, Percent_Identity=29.8657718120805, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=275, Percent_Identity=29.8181818181818, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=400, Percent_Identity=62.75, Blast_Score=504, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6325337, Length=348, Percent_Identity=35.0574712643678, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=348, Percent_Identity=35.0574712643678, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=362, Percent_Identity=27.0718232044199, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320377, Length=357, Percent_Identity=27.7310924369748, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=309, Percent_Identity=28.1553398058252, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=248, Percent_Identity=27.0161290322581, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=59.0909090909091, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=59.0909090909091, Blast_Score=478, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=50.3856041131105, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553807, Length=349, Percent_Identity=34.6704871060172, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553816, Length=349, Percent_Identity=34.6704871060172, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651721, Length=349, Percent_Identity=34.6704871060172, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17864154, Length=349, Percent_Identity=34.6704871060172, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652838, Length=349, Percent_Identity=34.3839541547278, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=349, Percent_Identity=34.3839541547278, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922690, Length=304, Percent_Identity=30.9210526315789, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI45550900, Length=431, Percent_Identity=29.0023201856148, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI17137380, Length=339, Percent_Identity=30.3834808259587, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24647048, Length=369, Percent_Identity=25.7452574525745, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647046, Length=369, Percent_Identity=25.7452574525745, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI28572034, Length=166, Percent_Identity=34.9397590361446, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_PROAC (Q6A6L7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056554.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q6A6L7
- SMR:   Q6A6L7
- GeneID:   2931415
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1873
- NMPDR:   fig|267747.1.peg.1831
- HOGENOM:   HBG307581
- OMA:   FEGQVYV
- ProtClustDB:   PRK12736
- BioCyc:   PACN267747:PPA1873-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 44141; Mature: 44010

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHCNIGTIGHIDHGKTTLTAAISKVLHDKYPELNEESPFDQIDKAPEERQ
CCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
RGITISIAHIEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPAIVVALNKCDMVDDEELIELVEMEVRELLTSQEFDGDNCPVVRISAFQA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
LQGDEKWTQSILDLMDAVDEYIPQPERDLDKPFLMPIEDVFTITGRGTVVTGRVERGVVK
HCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHEEEEECCCEEEECCCCCCCCC
TGEEVEIVGIHEKTQKTTVTGVEMFRKILDEGRAGENVGVLLRGTKKEDVVRGMVLSKPG
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHEEECCC
STTPHTDFEGQVYVLKKDEGGRHKPFFSHYSPQFYFRTTDVTGTVELPEGTEMVMPGDNT
CCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCEEEEEEECCEEEEECCCCCEEEECCCCC
DMTVHLIHPVAMEDQLKFAIREGGRTVGAGRVTKIIK
CEEEEEECCCCCCHHHHHHHHCCCCEECCCHHHHHCC
>Mature Secondary Structure 
AKAKFERTKPHCNIGTIGHIDHGKTTLTAAISKVLHDKYPELNEESPFDQIDKAPEERQ
CCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHH
RGITISIAHIEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTRE
CCEEEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHH
HVLLARQVGVPAIVVALNKCDMVDDEELIELVEMEVRELLTSQEFDGDNCPVVRISAFQA
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHH
LQGDEKWTQSILDLMDAVDEYIPQPERDLDKPFLMPIEDVFTITGRGTVVTGRVERGVVK
HCCCHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHEEEEECCCEEEECCCCCCCCC
TGEEVEIVGIHEKTQKTTVTGVEMFRKILDEGRAGENVGVLLRGTKKEDVVRGMVLSKPG
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHEEECCC
STTPHTDFEGQVYVLKKDEGGRHKPFFSHYSPQFYFRTTDVTGTVELPEGTEMVMPGDNT
CCCCCCCCCCEEEEEEECCCCCCCCCHHCCCCEEEEEEECCEEEEECCCCCEEEECCCCC
DMTVHLIHPVAMEDQLKFAIREGGRTVGAGRVTKIIK
CEEEEEECCCCCCHHHHHHHHCCCCEECCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA