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The map label for this gene is recG [H]

Identifier: 50842945

GI number: 50842945

Start: 1581410

End: 1583659

Strand: Reverse

Name: recG [H]

Synonym: PPA1466

Alternate gene names: 50842945

Gene position: 1583659-1581410 (Counterclockwise)

Preceding gene: 50842946

Following gene: 50842943

Centisome position: 61.86

GC content: 61.11

Gene sequence:

>2250_bases
ATGGGTCGATACGGTGACGATGTGAATGCAGTGACCAGGTGGCGTACCCGCACCCAGAAGGAGCTTCATCGTCCCTTAGC
GGAGGCGGTGGGAGCCAAGACGGCCAAGGCCTTCTCTGCGCTGCATGTTGGCACGGTGGGAGAGGCCCTCGGTCATCTTC
CGCGTCGCTACCTTACCGGCACCGAAACCACCGACTTGTCCCACTTAGTCATTGACACTGAAGTCGCCTTGGTGGCCGAT
GTTGTCGGCGTCGAGGTTCACACCAACAAGACTGTCACTGGCAGGGAGAGACGCCCGCGTCAACGTCTGGAAGTCATGCT
TTCTGACGGTAAAGCGCGCCTGCCCGTTACGTTCTTCGGCAAACCCCACATCGTTTCCTACTGGCAACGCATTTTTTCGG
CCCATACTCACGGCATCTTTGTCGGCAAGGTGGGGGAGTTCCGCGGGAACCCGCAGCTCGTCCACCCCGACTTCGTCATG
ATTGACCGCAACGGGAAGGTCGTGGCAGGTCGCGAGGAGGGCAAGGTGATGGCTGCCCAGGTACAACGTCATGGTTTGCT
GGGCCTTTACCCGCAGACGTCGAAATTGCGTACCTGGGAAATCGCCTCAGTGGAGTCGATGCTGCTCGAATCCACGCCCG
AACTCGAGGACACCCTGCCTGAATGGTTACGTCAAGAAGCTGACCTACCGAGTTTATGGGAGGCTTACCACGCCATCCAT
TACCCGCATTCGGTGGCTGAGGCTGATCGCGGCGCCGAACGACTTATCTGGGAAGAGGCGATAGCCACCCAGGTGACGAT
GGCGGTGCGACGCCGCAGTGCAGAGCGTCATGATGCCCCGGTGTGTTCGCGTCGCGACGGCGGATTGCTGGTAGCCTTTG
AGGATCGCCTGCCCTTTACCCCGACTGTCGGCCAGGACGAGGTGAGTCGGGTGATTGACGCCGACCTCTCGGCAGATCGT
CCCATGCACCGATTATTGCAAGGAGAAGTTGGCTCGGGGAAAACCTTCGTTGCGCTGCGAGCGATGTTGCAGGTTGTTGA
CGCTGGGCACCAGGCTGTCCTGCTGGCGCCGACCGAAGTCCTTGCTCAGCAGCATTACCGAACCATTATCAATATGTTGG
GCGACTTGGCGTCCGGCGAGGGATTGGATGCCCCGGAGATCTCCACGGGTGTGGCGCTACTGACCGGCTCGATGAAGGCT
GCGGGCACCCGGGCGGCACTGGCTGATATTGCCTCAGGAGCCGCTGGAATTATCGTCGGCACCCATTCCCTGCTGTCGGG
CCGAGTGATTTACAACGACATTGGATTAGTCGTCGTCGATGAGCAGCATCGCTTCGGCGTTGAGCAACGCAGTGTTCTCA
CTACTGGGGAGGGCGCACGCCCCCACGAACTCGTTCTCACCGCGACCCCGATTCCGCGAACGGTGGCGATGACGGTCTTC
GGTGACCTCGAGGTCTCGAGCTTGAGAGAATTACCGACTGGACGTGCCGACGTTCAGACCACGGTCGTCGACCTGCCCGC
CCACGGATCCTGGCTCACCCGCGCCTGGCAGCGGATCCGGGAGGAATGTGATGCCGGACATCAGGTGTTTGTGGTCTGCC
CTCGCATCAATTCCGACGACGCTGACGACGTCGAAGGTGGGCGCCCTCCCGCAGCTGCCGTCGAAGAGCTCGCTCCTCAA
CTGGCGACGGGACCTCTCGCCGGATTAAGAGTTGAGGCCCTGCACTCCCGGCTAGATTCGTCCGAGAAAGACCTCGTTAT
GGACAGGTTTATCAAGGGTGAGTCGCAGGTGCTTATCTCCACAACGGTGATTGAGGTCGGCGTCGACGTCCCCAATGCCA
CCATGATGGTCATCATGGACGCCGACCGGTTTGGCGTCTCGCAGCTGCACCAGTTGCGCGGTCGGATCGGTCGAGGCAAC
CTGCCGGGGCTCTGCCTGCTGGTGACGACGGCGCCGCCCGGATCGGTGGCGCGCGAGCGTTTGGACGCCGTGGCCGCGTC
CCGTGATGGATTTGAACTAGCCGAACTTGACCTAGCTCAGCGTCATGAAGGCAATGTGCTGGGATCCTCTCAAGCCGGTT
ATTCGTCCCCGCTACGCCTGTTGCGGGTGCTCGACCACGCTGAGATTGTGACGGCATCGAAAGACCTGGCCGAACGGTGG
GTGGCCGAAGCCCCCGATGATCCGAGACTCCTTGACGTCGTGACCGCCACCGAGATGCTCGCTGAGGGTGACCTCATGGA
ACAGGGTTGA

Upstream 100 bases:

>100_bases
CGCATGAACGTGTGCACTTCGTGCCTCAAGGCTGGTCGAGTCAGCCGCTGAGCGCCGCGTTTGACGATGGAACCCGCCGT
AAGGCGGGTTCTTTGTCGTT

Downstream 100 bases:

>100_bases
GCCGCCCCCGTTAAAGAAGACTGAGCCGACCCTCCGCCACGTCGGCTATGAAGCATCATGAAGGCCCGAACCGCGAGAGC
GATCCGGGCCTACTGAAATA

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MGRYGDDVNAVTRWRTRTQKELHRPLAEAVGAKTAKAFSALHVGTVGEALGHLPRRYLTGTETTDLSHLVIDTEVALVAD
VVGVEVHTNKTVTGRERRPRQRLEVMLSDGKARLPVTFFGKPHIVSYWQRIFSAHTHGIFVGKVGEFRGNPQLVHPDFVM
IDRNGKVVAGREEGKVMAAQVQRHGLLGLYPQTSKLRTWEIASVESMLLESTPELEDTLPEWLRQEADLPSLWEAYHAIH
YPHSVAEADRGAERLIWEEAIATQVTMAVRRRSAERHDAPVCSRRDGGLLVAFEDRLPFTPTVGQDEVSRVIDADLSADR
PMHRLLQGEVGSGKTFVALRAMLQVVDAGHQAVLLAPTEVLAQQHYRTIINMLGDLASGEGLDAPEISTGVALLTGSMKA
AGTRAALADIASGAAGIIVGTHSLLSGRVIYNDIGLVVVDEQHRFGVEQRSVLTTGEGARPHELVLTATPIPRTVAMTVF
GDLEVSSLRELPTGRADVQTTVVDLPAHGSWLTRAWQRIREECDAGHQVFVVCPRINSDDADDVEGGRPPAAAVEELAPQ
LATGPLAGLRVEALHSRLDSSEKDLVMDRFIKGESQVLISTTVIEVGVDVPNATMMVIMDADRFGVSQLHQLRGRIGRGN
LPGLCLLVTTAPPGSVARERLDAVAASRDGFELAELDLAQRHEGNVLGSSQAGYSSPLRLLRVLDHAEIVTASKDLAERW
VAEAPDDPRLLDVVTATEMLAEGDLMEQG

Sequences:

>Translated_749_residues
MGRYGDDVNAVTRWRTRTQKELHRPLAEAVGAKTAKAFSALHVGTVGEALGHLPRRYLTGTETTDLSHLVIDTEVALVAD
VVGVEVHTNKTVTGRERRPRQRLEVMLSDGKARLPVTFFGKPHIVSYWQRIFSAHTHGIFVGKVGEFRGNPQLVHPDFVM
IDRNGKVVAGREEGKVMAAQVQRHGLLGLYPQTSKLRTWEIASVESMLLESTPELEDTLPEWLRQEADLPSLWEAYHAIH
YPHSVAEADRGAERLIWEEAIATQVTMAVRRRSAERHDAPVCSRRDGGLLVAFEDRLPFTPTVGQDEVSRVIDADLSADR
PMHRLLQGEVGSGKTFVALRAMLQVVDAGHQAVLLAPTEVLAQQHYRTIINMLGDLASGEGLDAPEISTGVALLTGSMKA
AGTRAALADIASGAAGIIVGTHSLLSGRVIYNDIGLVVVDEQHRFGVEQRSVLTTGEGARPHELVLTATPIPRTVAMTVF
GDLEVSSLRELPTGRADVQTTVVDLPAHGSWLTRAWQRIREECDAGHQVFVVCPRINSDDADDVEGGRPPAAAVEELAPQ
LATGPLAGLRVEALHSRLDSSEKDLVMDRFIKGESQVLISTTVIEVGVDVPNATMMVIMDADRFGVSQLHQLRGRIGRGN
LPGLCLLVTTAPPGSVARERLDAVAASRDGFELAELDLAQRHEGNVLGSSQAGYSSPLRLLRVLDHAEIVTASKDLAERW
VAEAPDDPRLLDVVTATEMLAEGDLMEQG
>Mature_748_residues
GRYGDDVNAVTRWRTRTQKELHRPLAEAVGAKTAKAFSALHVGTVGEALGHLPRRYLTGTETTDLSHLVIDTEVALVADV
VGVEVHTNKTVTGRERRPRQRLEVMLSDGKARLPVTFFGKPHIVSYWQRIFSAHTHGIFVGKVGEFRGNPQLVHPDFVMI
DRNGKVVAGREEGKVMAAQVQRHGLLGLYPQTSKLRTWEIASVESMLLESTPELEDTLPEWLRQEADLPSLWEAYHAIHY
PHSVAEADRGAERLIWEEAIATQVTMAVRRRSAERHDAPVCSRRDGGLLVAFEDRLPFTPTVGQDEVSRVIDADLSADRP
MHRLLQGEVGSGKTFVALRAMLQVVDAGHQAVLLAPTEVLAQQHYRTIINMLGDLASGEGLDAPEISTGVALLTGSMKAA
GTRAALADIASGAAGIIVGTHSLLSGRVIYNDIGLVVVDEQHRFGVEQRSVLTTGEGARPHELVLTATPIPRTVAMTVFG
DLEVSSLRELPTGRADVQTTVVDLPAHGSWLTRAWQRIREECDAGHQVFVVCPRINSDDADDVEGGRPPAAAVEELAPQL
ATGPLAGLRVEALHSRLDSSEKDLVMDRFIKGESQVLISTTVIEVGVDVPNATMMVIMDADRFGVSQLHQLRGRIGRGNL
PGLCLLVTTAPPGSVARERLDAVAASRDGFELAELDLAQRHEGNVLGSSQAGYSSPLRLLRVLDHAEIVTASKDLAERWV
AEAPDDPRLLDVVTATEMLAEGDLMEQG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=730, Percent_Identity=33.8356164383562, Blast_Score=308, Evalue=7e-85,
Organism=Escherichia coli, GI1787357, Length=406, Percent_Identity=34.4827586206897, Blast_Score=203, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81402; Mature: 81270

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRYGDDVNAVTRWRTRTQKELHRPLAEAVGAKTAKAFSALHVGTVGEALGHLPRRYLTG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHCCC
TETTDLSHLVIDTEVALVADVVGVEVHTNKTVTGRERRPRQRLEVMLSDGKARLPVTFFG
CCCCCCEEEEEECHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECC
KPHIVSYWQRIFSAHTHGIFVGKVGEFRGNPQLVHPDFVMIDRNGKVVAGREEGKVMAAQ
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCEEEEECCCCEEECCCCCCEEHHH
VQRHGLLGLYPQTSKLRTWEIASVESMLLESTPELEDTLPEWLRQEADLPSLWEAYHAIH
HHHCCCEEECCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
YPHSVAEADRGAERLIWEEAIATQVTMAVRRRSAERHDAPVCSRRDGGLLVAFEDRLPFT
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCC
PTVGQDEVSRVIDADLSADRPMHRLLQGEVGSGKTFVALRAMLQVVDAGHQAVLLAPTEV
CCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHH
LAQQHYRTIINMLGDLASGEGLDAPEISTGVALLTGSMKAAGTRAALADIASGAAGIIVG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHCCCCEEEEE
THSLLSGRVIYNDIGLVVVDEQHRFGVEQRSVLTTGEGARPHELVLTATPIPRTVAMTVF
CHHHHCCEEEEECCCEEEECCHHHCCCCHHCEEECCCCCCCCEEEEEECCCCCEEEEEEE
GDLEVSSLRELPTGRADVQTTVVDLPAHGSWLTRAWQRIREECDAGHQVFVVCPRINSDD
CCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
ADDVEGGRPPAAAVEELAPQLATGPLAGLRVEALHSRLDSSEKDLVMDRFIKGESQVLIS
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
TTVIEVGVDVPNATMMVIMDADRFGVSQLHQLRGRIGRGNLPGLCLLVTTAPPGSVARER
EEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHH
LDAVAASRDGFELAELDLAQRHEGNVLGSSQAGYSSPLRLLRVLDHAEIVTASKDLAERW
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEAPDDPRLLDVVTATEMLAEGDLMEQG
HHCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GRYGDDVNAVTRWRTRTQKELHRPLAEAVGAKTAKAFSALHVGTVGEALGHLPRRYLTG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHCCC
TETTDLSHLVIDTEVALVADVVGVEVHTNKTVTGRERRPRQRLEVMLSDGKARLPVTFFG
CCCCCCEEEEEECHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCEEECC
KPHIVSYWQRIFSAHTHGIFVGKVGEFRGNPQLVHPDFVMIDRNGKVVAGREEGKVMAAQ
CCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEECCCEEEEECCCCEEECCCCCCEEHHH
VQRHGLLGLYPQTSKLRTWEIASVESMLLESTPELEDTLPEWLRQEADLPSLWEAYHAIH
HHHCCCEEECCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
YPHSVAEADRGAERLIWEEAIATQVTMAVRRRSAERHDAPVCSRRDGGLLVAFEDRLPFT
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCC
PTVGQDEVSRVIDADLSADRPMHRLLQGEVGSGKTFVALRAMLQVVDAGHQAVLLAPTEV
CCCCHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHH
LAQQHYRTIINMLGDLASGEGLDAPEISTGVALLTGSMKAAGTRAALADIASGAAGIIVG
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHCCHHHHHHHHHCCCCEEEEE
THSLLSGRVIYNDIGLVVVDEQHRFGVEQRSVLTTGEGARPHELVLTATPIPRTVAMTVF
CHHHHCCEEEEECCCEEEECCHHHCCCCHHCEEECCCCCCCCEEEEEECCCCCEEEEEEE
GDLEVSSLRELPTGRADVQTTVVDLPAHGSWLTRAWQRIREECDAGHQVFVVCPRINSDD
CCCCHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
ADDVEGGRPPAAAVEELAPQLATGPLAGLRVEALHSRLDSSEKDLVMDRFIKGESQVLIS
CCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
TTVIEVGVDVPNATMMVIMDADRFGVSQLHQLRGRIGRGNLPGLCLLVTTAPPGSVARER
EEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHH
LDAVAASRDGFELAELDLAQRHEGNVLGSSQAGYSSPLRLLRVLDHAEIVTASKDLAERW
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VAEAPDDPRLLDVVTATEMLAEGDLMEQG
HHCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]