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The map label for this gene is rsmD [H]

Identifier: 50842943

GI number: 50842943

Start: 1580139

End: 1580747

Strand: Reverse

Name: rsmD [H]

Synonym: PPA1464

Alternate gene names: 50842943

Gene position: 1580747-1580139 (Counterclockwise)

Preceding gene: 50842945

Following gene: 50842942

Centisome position: 61.74

GC content: 60.26

Gene sequence:

>609_bases
GTGAGCAGGATTATTGCGGGACGTGCCGGCGGGAAACGTCTGACTACCCCGCCGGGACGACTGACCCGTCCGACGACCGA
CCGGGTACGCGAGGCTGTGTTCTCCGCTTTGGCAGCATGGAACGGGACCGCTGACGAGGTTCCCGAGGACCAATTGGCCG
GTCAGACATTCTGCGATTTGTTCGCTGGTTCAGGTGCCGTGGCCTTAGAGGCTGCCAGTAGGGGGGCCACAACGGTGGTT
GCCGTCGACCGCGATCGCTGCGCCTGCAATGTCATGAAAGATAATTCTCGGACAACCCGGTTGAGGATCGAAGTGTTATC
CCAGACGGTGACGGCTTTCCTCGCCGAGAACCACCGCGTCTTTGATGTCGTTTGGTTCGATCCGCCCTACGACCTCGCCG
CCGATGATATGGATAGCCTCATCTTGACAGCGTGCGACGGGGCCCTCGCCCACAACGGTCTGCTAGTGGTTGAGAGATCA
CGGAGAAGTCGGGATCCCTGTTTTCCGGATGGATACGAGTCGTGGAATTCTCGCTACGGCGAGACAGTTGTCTATTACGC
CCAACGTACCGGTGAGCCGGAGGCAGATGCGGGCAGGGAGCAGCAGTGA

Upstream 100 bases:

>100_bases
TCGACTGGCACAAAGTTACCGAGGTTCTTCACCTTGGAATATCACATGACCCCTGCACGCGGCTGGAGAAATGGGAATCT
GGTACCGCTAGCCTGGAGCA

Downstream 100 bases:

>100_bases
AAGCAGTCTTTTCAGGGTCATTCGACCCCATCACCCTCGGTCACGTTGACATCGTGACCCGAGCAGCCGAACTCATCGAC
GAGGTCGTCGTCGGAGTGGC

Product: putative methylase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MSRIIAGRAGGKRLTTPPGRLTRPTTDRVREAVFSALAAWNGTADEVPEDQLAGQTFCDLFAGSGAVALEAASRGATTVV
AVDRDRCACNVMKDNSRTTRLRIEVLSQTVTAFLAENHRVFDVVWFDPPYDLAADDMDSLILTACDGALAHNGLLVVERS
RRSRDPCFPDGYESWNSRYGETVVYYAQRTGEPEADAGREQQ

Sequences:

>Translated_202_residues
MSRIIAGRAGGKRLTTPPGRLTRPTTDRVREAVFSALAAWNGTADEVPEDQLAGQTFCDLFAGSGAVALEAASRGATTVV
AVDRDRCACNVMKDNSRTTRLRIEVLSQTVTAFLAENHRVFDVVWFDPPYDLAADDMDSLILTACDGALAHNGLLVVERS
RRSRDPCFPDGYESWNSRYGETVVYYAQRTGEPEADAGREQQ
>Mature_201_residues
SRIIAGRAGGKRLTTPPGRLTRPTTDRVREAVFSALAAWNGTADEVPEDQLAGQTFCDLFAGSGAVALEAASRGATTVVA
VDRDRCACNVMKDNSRTTRLRIEVLSQTVTAFLAENHRVFDVVWFDPPYDLAADDMDSLILTACDGALAHNGLLVVERSR
RSRDPCFPDGYESWNSRYGETVVYYAQRTGEPEADAGREQQ

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=197, Percent_Identity=30.4568527918782, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22091; Mature: 21960

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIIAGRAGGKRLTTPPGRLTRPTTDRVREAVFSALAAWNGTADEVPEDQLAGQTFCDL
CCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FAGSGAVALEAASRGATTVVAVDRDRCACNVMKDNSRTTRLRIEVLSQTVTAFLAENHRV
HCCCCEEEEEECCCCCEEEEEECCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCEE
FDVVWFDPPYDLAADDMDSLILTACDGALAHNGLLVVERSRRSRDPCFPDGYESWNSRYG
EEEEEECCCCCCCCCCHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCHHHHHHCCC
ETVVYYAQRTGEPEADAGREQQ
CEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SRIIAGRAGGKRLTTPPGRLTRPTTDRVREAVFSALAAWNGTADEVPEDQLAGQTFCDL
CCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
FAGSGAVALEAASRGATTVVAVDRDRCACNVMKDNSRTTRLRIEVLSQTVTAFLAENHRV
HCCCCEEEEEECCCCCEEEEEECCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCEE
FDVVWFDPPYDLAADDMDSLILTACDGALAHNGLLVVERSRRSRDPCFPDGYESWNSRYG
EEEEEECCCCCCCCCCHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCHHHHHHCCC
ETVVYYAQRTGEPEADAGREQQ
CEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]