Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 50842919

GI number: 50842919

Start: 1557240

End: 1557959

Strand: Reverse

Name: trmD

Synonym: PPA1440

Alternate gene names: 50842919

Gene position: 1557959-1557240 (Counterclockwise)

Preceding gene: 50842920

Following gene: 50842918

Centisome position: 60.85

GC content: 60.14

Gene sequence:

>720_bases
ATGAGGCTGGACTACCTGTCAATTTTTCCGGCGTACTTTGATGTCCTCGACATATCTTTGCTGGGTAAGGCGGCAGTCAA
CGGTCTGGTGGAGGTCCACGCCCACGACTTGCGCGACTGGACCCACGATCGCCATCGCACCGTGGACGACACCCCGTGTG
GCGGAGGCGCGGGCATGGTCATGAAGCCTGACCCTTGGGGGGAAGCTTTCGACGAGATCATTGGCACTGAGCCGGACTCG
TCGGTTCATGTGATTTTCCCTAGCCCGTCAGGTGCGCCATTTACCCAGTCTGCTGCTCAAGAGTTGTCGTCGGCGGTGCG
GATCGTGTTCTGTTGCGGACGGTACGAGGGGATCGACCACCGCGTGATCGACTATGCGCGGTCGATGTGGACTGTTCATG
AGGTGAGCCTGGGAGACTACGTGCTTAATGGGGGAGAAGTAGCGGCTCTGGCTATCACTGAGGCCGTGGTTCGGCTGGTG
CCCGGGTTCATGGGTAACCCGGAGTCTCTAGCGGAGGAGTCGTTCTCGGCCGGGCAAGACGGCTTGCTGGAGTATCCGCT
GTTCACCCGGCCGGTCTCATGGCGTGGCCTCGATGTCCCCGAGGTTCTCATGAGTGGTCATCATGGTCGAATTGCGAAGT
GGCGCCGTGATGAGTCAGTCCGCCTGACGTGTGAGCGGCGTCCTGATCTGACGGATTCCGACTTCGGTGTTCCCCATTGA

Upstream 100 bases:

>100_bases
TCCACCAACTCGTCCCTACTGTCGATGTGGCGTCCGGGCGGATCGTCGTCAGTGGCATTCCTGGCTTGCTCGATTCGAAC
GCTGAAGAGGCGAGGTGACG

Downstream 100 bases:

>100_bases
AGCGTCTCTAATCGACAAAATTCGAACCGAAAATGTTGCGATAATCAAGAGTAAAACCCTTAGTGAACCAAGGGTCGAGG
GAAGTTTTCGAAAACACAGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRLDYLSIFPAYFDVLDISLLGKAAVNGLVEVHAHDLRDWTHDRHRTVDDTPCGGGAGMVMKPDPWGEAFDEIIGTEPDS
SVHVIFPSPSGAPFTQSAAQELSSAVRIVFCCGRYEGIDHRVIDYARSMWTVHEVSLGDYVLNGGEVAALAITEAVVRLV
PGFMGNPESLAEESFSAGQDGLLEYPLFTRPVSWRGLDVPEVLMSGHHGRIAKWRRDESVRLTCERRPDLTDSDFGVPH

Sequences:

>Translated_239_residues
MRLDYLSIFPAYFDVLDISLLGKAAVNGLVEVHAHDLRDWTHDRHRTVDDTPCGGGAGMVMKPDPWGEAFDEIIGTEPDS
SVHVIFPSPSGAPFTQSAAQELSSAVRIVFCCGRYEGIDHRVIDYARSMWTVHEVSLGDYVLNGGEVAALAITEAVVRLV
PGFMGNPESLAEESFSAGQDGLLEYPLFTRPVSWRGLDVPEVLMSGHHGRIAKWRRDESVRLTCERRPDLTDSDFGVPH
>Mature_239_residues
MRLDYLSIFPAYFDVLDISLLGKAAVNGLVEVHAHDLRDWTHDRHRTVDDTPCGGGAGMVMKPDPWGEAFDEIIGTEPDS
SVHVIFPSPSGAPFTQSAAQELSSAVRIVFCCGRYEGIDHRVIDYARSMWTVHEVSLGDYVLNGGEVAALAITEAVVRLV
PGFMGNPESLAEESFSAGQDGLLEYPLFTRPVSWRGLDVPEVLMSGHHGRIAKWRRDESVRLTCERRPDLTDSDFGVPH

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=234, Percent_Identity=40.5982905982906, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PROAC (Q6A7S7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056146.1
- ProteinModelPortal:   Q6A7S7
- SMR:   Q6A7S7
- GeneID:   2931807
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1440
- NMPDR:   fig|267747.1.peg.1423
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   CLSK2763979
- BioCyc:   PACN267747:PPA1440-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26350; Mature: 26350

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDYLSIFPAYFDVLDISLLGKAAVNGLVEVHAHDLRDWTHDRHRTVDDTPCGGGAGMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
MKPDPWGEAFDEIIGTEPDSSVHVIFPSPSGAPFTQSAAQELSSAVRIVFCCGRYEGIDH
ECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCH
RVIDYARSMWTVHEVSLGDYVLNGGEVAALAITEAVVRLVPGFMGNPESLAEESFSAGQD
HHHHHHHHHHHHEEECCCCEEECCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
GLLEYPLFTRPVSWRGLDVPEVLMSGHHGRIAKWRRDESVRLTCERRPDLTDSDFGVPH
CCEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLDYLSIFPAYFDVLDISLLGKAAVNGLVEVHAHDLRDWTHDRHRTVDDTPCGGGAGMV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
MKPDPWGEAFDEIIGTEPDSSVHVIFPSPSGAPFTQSAAQELSSAVRIVFCCGRYEGIDH
ECCCCCHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEEEECCCCCCCH
RVIDYARSMWTVHEVSLGDYVLNGGEVAALAITEAVVRLVPGFMGNPESLAEESFSAGQD
HHHHHHHHHHHHEEECCCCEEECCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
GLLEYPLFTRPVSWRGLDVPEVLMSGHHGRIAKWRRDESVRLTCERRPDLTDSDFGVPH
CCEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA