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The map label for this gene is pntA

Identifier: 50842112

GI number: 50842112

Start: 690354

End: 692018

Strand: Direct

Name: pntA

Synonym: PPA0625

Alternate gene names: 50842112

Gene position: 690354-692018 (Clockwise)

Preceding gene: 50842109

Following gene: 50842113

Centisome position: 26.96

GC content: 60.78

Gene sequence:

>1665_bases
ATGCGGTGGGTTACTGCAAGTGGGGTGCGCCATTTTTCACTCCACATAGCCCCCAATCTGGGGAAACGTGCAACCCCGGT
GTCGTGGGTAAGGACGTTTGGCCGTAAAGTCACCATCATGATCATCGGAATCCCACGGGAGTTACTCCCAGGTGAGAATC
GGGTCGCCGCGACTCCGACGACTGTTCCCGCCCTACTCAAACTCGGCTACGAGGTGGTCGTCGAGGCCGGAGCTGGCGAG
CATGCTGCTCAGCCTGACCGCGCGTATGAAGAGGCCGGAGCAAGGATTGTCGAACCGGGGGAGGCGTGGAAGGCTGACCT
CGTACTGGCCGTCAACGAACCCACCGACGACCAGATCTCCCTCATGCGCGCAGGAGCCGTCCTCGTCACCTTTCTCCATC
CCCGTCAGGACCTCGCCCTTACCGAGAAGCTGGCCGCAGCTGGCGTCACCGGTCTCAGCATGGATATGGTCCCGCGCATT
TCGCGCGCCCAGTCGATGGACGCACTGTCTTCGATGGCCAATATCTCCGGTTACCGCGCCGTCGTCGAGGCGGCATTCGC
CTACGGACGCACCTTCGGTGGCCAGGTCACTGCGGCAGGCAAGGTTCCGCCGGCCAAAGTCTTCGTCATCGGTACTGGCG
TCGCCGGTTTGGCTGCACTGGGCGCCGCCAATTCGATGGGTGCCGAGGTTTACGCCACCGATGTCCGCCCCGAGACCGCT
GAGCAGGTGCAGTCGATGGGCGCCATCTTCCTACACGTGCGAACCGGAGATTCTGACCAAGGCGTCAGTTCTGACGGCTA
CGCCAAAGAGACGAGCGACGACTACAACGCCCGCGCCGCCGAGTTGTACATGGAACAGGCTGGCAAGGACGATGTCATCA
TCACCACGGCTGCTATCCCTGGCAAGCCGTCCCCCAAACTCATCACTGCCGAGATGGTCTACGCGATGAAGCCTGGCAGC
GTCATCGTTGACCTTGCTGCCCTAGGGGGTGGCAACTGCGAGTTGACTCGCCCCGGTGAGTCCTATGTCACCGATAACGG
TGTGACGATTATTGGTTACACTGATCTGCCGTCTCGCCTGCCTGGTCAGGCCAGCCAGCTTTACGGCACCAACCTCGTCA
ATTTGCTTAAACTGGCCACCCCGGAAAAGAACGGCGAGCTCGTCCTTAACTTTGACGACGAGGTCATCCGCACTATGACG
GTGTGCCACGAAGGTCAGGTGGCTTTCCCTCCACCTCCGGTACAGGTCGCTGCCGCGCCCAAGCCTGTCGCCAAAGCCGA
GATTGCCAGTGCTCCGGCCACCCCGGAGAAAAAGCCGCGCCCGTGGCCGCAGACCTTCGGCATCGTCGCAGTGCTGTCGA
TCGCGCTGATTGCCCTGTTGACCTTCTCCCCGATGGAATTCGTAGCCTTGTTTGGCACCTTCGCCATCGCGGTCGTCGTT
GGCTACTACGTGGTGTGGAACGTCACCCACGCACTCCACACCCCGCTTATGAGTGTCACGAACGCTATCAGCGGCATCAT
CATCGTTGGTGCGATCACCCAACTCGGCAGTGAATCGTGGGGTATCCGGATTGTCGCCGCGTTGACTGTGCTTATTGCCA
GTATCAACATCTTTGGCGGCTTCACGGTCACCCAGCGCATGCTCAAGATGTTCAGGAAGGCCTGA

Upstream 100 bases:

>100_bases
CGTCTCAGGACCAGTGGGGTCAGCCCCTTGCCCAGCCGCAGCCTTGGACCCGGCTTCTGACCTCAAGGGGCCTCATCCGA
GCAGCTGATTTCCTTCGAAG

Downstream 100 bases:

>100_bases
TCCATGACGTCAACACTTCTTATTGCCCCGTTGGCCGGGTCGTTGGTGAATTTCGTCAACGCATCCTTCATCGTCGCAGG
CCTACTGTTCATCTTTGCCC

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MRWVTASGVRHFSLHIAPNLGKRATPVSWVRTFGRKVTIMIIGIPRELLPGENRVAATPTTVPALLKLGYEVVVEAGAGE
HAAQPDRAYEEAGARIVEPGEAWKADLVLAVNEPTDDQISLMRAGAVLVTFLHPRQDLALTEKLAAAGVTGLSMDMVPRI
SRAQSMDALSSMANISGYRAVVEAAFAYGRTFGGQVTAAGKVPPAKVFVIGTGVAGLAALGAANSMGAEVYATDVRPETA
EQVQSMGAIFLHVRTGDSDQGVSSDGYAKETSDDYNARAAELYMEQAGKDDVIITTAAIPGKPSPKLITAEMVYAMKPGS
VIVDLAALGGGNCELTRPGESYVTDNGVTIIGYTDLPSRLPGQASQLYGTNLVNLLKLATPEKNGELVLNFDDEVIRTMT
VCHEGQVAFPPPPVQVAAAPKPVAKAEIASAPATPEKKPRPWPQTFGIVAVLSIALIALLTFSPMEFVALFGTFAIAVVV
GYYVVWNVTHALHTPLMSVTNAISGIIIVGAITQLGSESWGIRIVAALTVLIASINIFGGFTVTQRMLKMFRKA

Sequences:

>Translated_554_residues
MRWVTASGVRHFSLHIAPNLGKRATPVSWVRTFGRKVTIMIIGIPRELLPGENRVAATPTTVPALLKLGYEVVVEAGAGE
HAAQPDRAYEEAGARIVEPGEAWKADLVLAVNEPTDDQISLMRAGAVLVTFLHPRQDLALTEKLAAAGVTGLSMDMVPRI
SRAQSMDALSSMANISGYRAVVEAAFAYGRTFGGQVTAAGKVPPAKVFVIGTGVAGLAALGAANSMGAEVYATDVRPETA
EQVQSMGAIFLHVRTGDSDQGVSSDGYAKETSDDYNARAAELYMEQAGKDDVIITTAAIPGKPSPKLITAEMVYAMKPGS
VIVDLAALGGGNCELTRPGESYVTDNGVTIIGYTDLPSRLPGQASQLYGTNLVNLLKLATPEKNGELVLNFDDEVIRTMT
VCHEGQVAFPPPPVQVAAAPKPVAKAEIASAPATPEKKPRPWPQTFGIVAVLSIALIALLTFSPMEFVALFGTFAIAVVV
GYYVVWNVTHALHTPLMSVTNAISGIIIVGAITQLGSESWGIRIVAALTVLIASINIFGGFTVTQRMLKMFRKA
>Mature_554_residues
MRWVTASGVRHFSLHIAPNLGKRATPVSWVRTFGRKVTIMIIGIPRELLPGENRVAATPTTVPALLKLGYEVVVEAGAGE
HAAQPDRAYEEAGARIVEPGEAWKADLVLAVNEPTDDQISLMRAGAVLVTFLHPRQDLALTEKLAAAGVTGLSMDMVPRI
SRAQSMDALSSMANISGYRAVVEAAFAYGRTFGGQVTAAGKVPPAKVFVIGTGVAGLAALGAANSMGAEVYATDVRPETA
EQVQSMGAIFLHVRTGDSDQGVSSDGYAKETSDDYNARAAELYMEQAGKDDVIITTAAIPGKPSPKLITAEMVYAMKPGS
VIVDLAALGGGNCELTRPGESYVTDNGVTIIGYTDLPSRLPGQASQLYGTNLVNLLKLATPEKNGELVLNFDDEVIRTMT
VCHEGQVAFPPPPVQVAAAPKPVAKAEIASAPATPEKKPRPWPQTFGIVAVLSIALIALLTFSPMEFVALFGTFAIAVVV
GYYVVWNVTHALHTPLMSVTNAISGIIIVGAITQLGSESWGIRIVAALTVLIASINIFGGFTVTQRMLKMFRKA

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=534, Percent_Identity=43.6329588014981, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI122939153, Length=534, Percent_Identity=43.6329588014981, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1787887, Length=515, Percent_Identity=59.6116504854369, Blast_Score=580, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17550456, Length=549, Percent_Identity=45.1730418943534, Blast_Score=412, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 58488; Mature: 58488

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00836 ALADH_PNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWVTASGVRHFSLHIAPNLGKRATPVSWVRTFGRKVTIMIIGIPRELLPGENRVAATPT
CCEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCHHHCCCCCCEEECCC
TVPALLKLGYEVVVEAGAGEHAAQPDRAYEEAGARIVEPGEAWKADLVLAVNEPTDDQIS
HHHHHHHCCEEEEEECCCCCCCCCCCHHHHHCCCEEECCCCCCCEEEEEEECCCCCHHHH
LMRAGAVLVTFLHPRQDLALTEKLAAAGVTGLSMDMVPRISRAQSMDALSSMANISGYRA
HHHHCEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVEAAFAYGRTFGGQVTAAGKVPPAKVFVIGTGVAGLAALGAANSMGAEVYATDVRPETA
HHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCHHH
EQVQSMGAIFLHVRTGDSDQGVSSDGYAKETSDDYNARAAELYMEQAGKDDVIITTAAIP
HHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
GKPSPKLITAEMVYAMKPGSVIVDLAALGGGNCELTRPGESYVTDNGVTIIGYTDLPSRL
CCCCCCEEEEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCEECCCEEEEEECCCCCCC
PGQASQLYGTNLVNLLKLATPEKNGELVLNFDDEVIRTMTVCHEGQVAFPPPPVQVAAAP
CCCHHHHHCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCEECCCCCCCEEECCC
KPVAKAEIASAPATPEKKPRPWPQTFGIVAVLSIALIALLTFSPMEFVALFGTFAIAVVV
CCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GYYVVWNVTHALHTPLMSVTNAISGIIIVGAITQLGSESWGIRIVAALTVLIASINIFGG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
FTVTQRMLKMFRKA
HHHHHHHHHHHHCC
>Mature Secondary Structure
MRWVTASGVRHFSLHIAPNLGKRATPVSWVRTFGRKVTIMIIGIPRELLPGENRVAATPT
CCEEECCCCEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCHHHCCCCCCEEECCC
TVPALLKLGYEVVVEAGAGEHAAQPDRAYEEAGARIVEPGEAWKADLVLAVNEPTDDQIS
HHHHHHHCCEEEEEECCCCCCCCCCCHHHHHCCCEEECCCCCCCEEEEEEECCCCCHHHH
LMRAGAVLVTFLHPRQDLALTEKLAAAGVTGLSMDMVPRISRAQSMDALSSMANISGYRA
HHHHCEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVEAAFAYGRTFGGQVTAAGKVPPAKVFVIGTGVAGLAALGAANSMGAEVYATDVRPETA
HHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHCCCCCCCCEEEEECCCCHHH
EQVQSMGAIFLHVRTGDSDQGVSSDGYAKETSDDYNARAAELYMEQAGKDDVIITTAAIP
HHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECC
GKPSPKLITAEMVYAMKPGSVIVDLAALGGGNCELTRPGESYVTDNGVTIIGYTDLPSRL
CCCCCCEEEEEEEEEECCCCEEEEEECCCCCCEEEECCCCCCEECCCEEEEEECCCCCCC
PGQASQLYGTNLVNLLKLATPEKNGELVLNFDDEVIRTMTVCHEGQVAFPPPPVQVAAAP
CCCHHHHHCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHCCCEECCCCCCCEEECCC
KPVAKAEIASAPATPEKKPRPWPQTFGIVAVLSIALIALLTFSPMEFVALFGTFAIAVVV
CCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
GYYVVWNVTHALHTPLMSVTNAISGIIIVGAITQLGSESWGIRIVAALTVLIASINIFGG
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
FTVTQRMLKMFRKA
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]