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The map label for this gene is tmk [H]

Identifier: 50841735

GI number: 50841735

Start: 296471

End: 297157

Strand: Direct

Name: tmk [H]

Synonym: PPA0243

Alternate gene names: 50841735

Gene position: 296471-297157 (Clockwise)

Preceding gene: 50841734

Following gene: 50841736

Centisome position: 11.58

GC content: 62.3

Gene sequence:

>687_bases
ATGACTGGCAACAGCATCGCCGCAAGATCCTTAAGGCTATCCATGACTGATCAGACGGTGACTGGCCGCGATCTGGGCGG
TGTATTCGTTGTCTTCGAGGGTGGGGACGGTGCCGGTAAGACGACCCAGGCCCGACTACTTGACCAGTGGCTGACGACCG
AGGGAATCCCTCACCTCATGACTCGCGAGCCCGGGGATTCCTGGCTTGGGCAGCGGATCCGCGAGCTGGTGCTCTCTCCT
GGCTCCGGGCCGATCTCCTCGCGCGCTGAGGCTTTGCTGTACAACGCCGACAAGGCTCAACACGTCGACGAGGTTGTCAT
TCCGGCTTTGCGAGAGGGCAAGGTCGTCGTTTGCGACCGTTACGTTGACTCGACTATCGCCTATCAGGGGGCTGGCAGGG
CCCTCGACCCTGGCGAGGTCGGCCAGCTCGCGTGCTGGGCGACGACCGGCCTGGTACCTGATGTGACGGTTCTGCTCGAC
GTCGATCCCTGCGAAGGGGCCGGTAAGATCGCCGCCAAGGATCGTCTGGAAGCTGCTGGTGACGAGTTCCACCTTCGGGT
GCGTCAACATTTTCTTGACCTCGCTGCGGCCCATCCGCAGCGATACCTCGTGCTGAACGCGCGGAAGAGTCGCAAGGAGA
TCAGTGAGGCCATCCGTCAGCGCGTGACTGGATTGCTCAACCGGTGA

Upstream 100 bases:

>100_bases
GTTGACGTCTCCAAGATGTCTCCTCAGGAAGCTTTTGAGGCCGGACGCGAGGTAGAGCGGGCGTCGGCATCGTTGTTTGC
GGCCCGACAATCGTTTAGGG

Downstream 100 bases:

>100_bases
GGAATCGAGCGGTTACGATGTCGGGCCGATCTGCAACGATGATCTTGTGAGCGATGTGTTGACCGGCGTGTGGGCCGATC
TTGTGGGTCAGGAGAAGGCT

Product: putative thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTGNSIAARSLRLSMTDQTVTGRDLGGVFVVFEGGDGAGKTTQARLLDQWLTTEGIPHLMTREPGDSWLGQRIRELVLSP
GSGPISSRAEALLYNADKAQHVDEVVIPALREGKVVVCDRYVDSTIAYQGAGRALDPGEVGQLACWATTGLVPDVTVLLD
VDPCEGAGKIAAKDRLEAAGDEFHLRVRQHFLDLAAAHPQRYLVLNARKSRKEISEAIRQRVTGLLNR

Sequences:

>Translated_228_residues
MTGNSIAARSLRLSMTDQTVTGRDLGGVFVVFEGGDGAGKTTQARLLDQWLTTEGIPHLMTREPGDSWLGQRIRELVLSP
GSGPISSRAEALLYNADKAQHVDEVVIPALREGKVVVCDRYVDSTIAYQGAGRALDPGEVGQLACWATTGLVPDVTVLLD
VDPCEGAGKIAAKDRLEAAGDEFHLRVRQHFLDLAAAHPQRYLVLNARKSRKEISEAIRQRVTGLLNR
>Mature_227_residues
TGNSIAARSLRLSMTDQTVTGRDLGGVFVVFEGGDGAGKTTQARLLDQWLTTEGIPHLMTREPGDSWLGQRIRELVLSPG
SGPISSRAEALLYNADKAQHVDEVVIPALREGKVVVCDRYVDSTIAYQGAGRALDPGEVGQLACWATTGLVPDVTVLLDV
DPCEGAGKIAAKDRLEAAGDEFHLRVRQHFLDLAAAHPQRYLVLNARKSRKEISEAIRQRVTGLLNR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=202, Percent_Identity=36.6336633663366, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 24694; Mature: 24563

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGNSIAARSLRLSMTDQTVTGRDLGGVFVVFEGGDGAGKTTQARLLDQWLTTEGIPHLM
CCCCHHHHHEEEEEECCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHH
TREPGDSWLGQRIRELVLSPGSGPISSRAEALLYNADKAQHVDEVVIPALREGKVVVCDR
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEC
YVDSTIAYQGAGRALDPGEVGQLACWATTGLVPDVTVLLDVDPCEGAGKIAAKDRLEAAG
HHCCHHEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
DEFHLRVRQHFLDLAAAHPQRYLVLNARKSRKEISEAIRQRVTGLLNR
CHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGNSIAARSLRLSMTDQTVTGRDLGGVFVVFEGGDGAGKTTQARLLDQWLTTEGIPHLM
CCCHHHHHEEEEEECCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHH
TREPGDSWLGQRIRELVLSPGSGPISSRAEALLYNADKAQHVDEVVIPALREGKVVVCDR
CCCCCHHHHHHHHHHHHCCCCCCCCCHHHHEEEECCCHHHHHHHHHHHHHCCCCEEEEEC
YVDSTIAYQGAGRALDPGEVGQLACWATTGLVPDVTVLLDVDPCEGAGKIAAKDRLEAAG
HHCCHHEECCCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
DEFHLRVRQHFLDLAAAHPQRYLVLNARKSRKEISEAIRQRVTGLLNR
CHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA