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The map label for this gene is camA [H]

Identifier: 50841628

GI number: 50841628

Start: 167045

End: 168424

Strand: Direct

Name: camA [H]

Synonym: PPA0139

Alternate gene names: 50841628

Gene position: 167045-168424 (Clockwise)

Preceding gene: 50841627

Following gene: 50841629

Centisome position: 6.52

GC content: 62.39

Gene sequence:

>1380_bases
GTGCCTTGTCAGGGGGTTTACGCGTGGTGTGGTAATCCGGTTTCTGGTGGCGACGGCTGCCACCCCTCGTGGCAAGACAT
GCCGTTGCGTGCCGATATGCCATACGAAGCTTGGCCTAGTGCGAAAAGCTCGCTGGAACCCTCGAAGAGGCAGGGTCGGC
AGGTTACCCTGGTTGGTGTATGCATCGTTTCGACGATGAACCCCATTCTGGGAGCAGATATGACGACCTACCAGTACCTC
ATCGTCGGTGGCGGGATGGCCGCTGATTCTGCCGCCCGCGGTATCCGCGACATCGACAAGAAAGGGTCGATCGCCATCCT
CAGCGCTGACGTCGACGCCCCGTATCCTCGGCCAGCGCTGAGCAAGAAGCTGTGGACTGACCCTGAGTTCACCTGGGACC
AGGCCGACCTCGCTACCGTCGCAGACACCGGCGCGGAATTGCGGCTCGGCACTGAGGTGCTCAGCATTGACCGTGACGGC
AAGACCGTCCTGACCGCTTCCGGCCAGGTATTCGGCTACCAGAAGTTGCTGCTCGTTACCGGCCTTACCCCGTCGCGCAT
TGACGACGACGGCGATGCCGTCCTCTACTTTCGGTCTGCTCGGGACTACCAGCAGCTGCGTGCGCTGGCCCAGCCCGGTC
ACCAGTTCGTGGTCGTCGGCGGCGGATATATCGGTGCCGAGCTTGCTGCGGGCCTCGTCCAGCAGGGCTGCGAGGTCGCC
CTCGTCACCCCCGATCCGACGCTGGGTGGTTCGCAATTCCCGGCCCAGATTGCGTCTGAGTACCAGAAGCTTTTCGCCGA
TGCTGGGGTGCACCTCGTCACTGGGAAGCGCGTCTGTTCGGTGCGCAAACATGAAGCGGCCGAGGTCACCCTCGATGACG
GCACTATTCTCCAGGCTGACGATGTTATTGCGGGTTTGGGGGCATCCCCGGTCACCAAACTGGCCGAGGAAGCGGGTCTT
ACCGTTAATGATGGGGTCATCGTTGATGAGCAGTTGCGCACCAACGATCCGGCCATCTGGGCCGCCGGAGACATCGCCAA
TTACCCGGACCCGGTGTTGGGCCGCACCCGCGTCGAGCATGTGGACAATGCCATCGCGATGGGCAAGGCGGCCGGCCGCA
TCATGGCGGGATCAAAAGACTCCTACACCCATACCCCGATGATGTACTCACAAATCTTTGGGGTCCGCTGGGAGGCAGTC
GGCACCCTCGACTCCAGCCTCGCGACCACATCAGTGGAAGCTGGGGATGGGCAGGTCGTCTACTACCTGTCCGACGGCAA
GCCTGTCGGTGTGCTGCTGTGGAACCTTCCCGGACGCACCGACAAGGCCGTCACAGTGCTTGCCGATCCGCCTGAGGACC
TCTCGACCGCTATCAGCTAA

Upstream 100 bases:

>100_bases
CCAGGCGAAGATGAAGGATGTCGACTCCGTTGCCGAGGAGGGTGACGCCTGACCTCACTCCCAACCCATTGCGAGGTCCG
TCCCGTTTGGGGTGGGCCTC

Downstream 100 bases:

>100_bases
CCACTAGGAGGAATCATGGATCTTGGACTCAACGGACGCACTGCGCTCATCACTGGCGCTGATTCGGGTATTGGCTGGCA
CACCGCGAAACTCCTCCTCG

Product: reductase, ferredoxin

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MPCQGVYAWCGNPVSGGDGCHPSWQDMPLRADMPYEAWPSAKSSLEPSKRQGRQVTLVGVCIVSTMNPILGADMTTYQYL
IVGGGMAADSAARGIRDIDKKGSIAILSADVDAPYPRPALSKKLWTDPEFTWDQADLATVADTGAELRLGTEVLSIDRDG
KTVLTASGQVFGYQKLLLVTGLTPSRIDDDGDAVLYFRSARDYQQLRALAQPGHQFVVVGGGYIGAELAAGLVQQGCEVA
LVTPDPTLGGSQFPAQIASEYQKLFADAGVHLVTGKRVCSVRKHEAAEVTLDDGTILQADDVIAGLGASPVTKLAEEAGL
TVNDGVIVDEQLRTNDPAIWAAGDIANYPDPVLGRTRVEHVDNAIAMGKAAGRIMAGSKDSYTHTPMMYSQIFGVRWEAV
GTLDSSLATTSVEAGDGQVVYYLSDGKPVGVLLWNLPGRTDKAVTVLADPPEDLSTAIS

Sequences:

>Translated_459_residues
MPCQGVYAWCGNPVSGGDGCHPSWQDMPLRADMPYEAWPSAKSSLEPSKRQGRQVTLVGVCIVSTMNPILGADMTTYQYL
IVGGGMAADSAARGIRDIDKKGSIAILSADVDAPYPRPALSKKLWTDPEFTWDQADLATVADTGAELRLGTEVLSIDRDG
KTVLTASGQVFGYQKLLLVTGLTPSRIDDDGDAVLYFRSARDYQQLRALAQPGHQFVVVGGGYIGAELAAGLVQQGCEVA
LVTPDPTLGGSQFPAQIASEYQKLFADAGVHLVTGKRVCSVRKHEAAEVTLDDGTILQADDVIAGLGASPVTKLAEEAGL
TVNDGVIVDEQLRTNDPAIWAAGDIANYPDPVLGRTRVEHVDNAIAMGKAAGRIMAGSKDSYTHTPMMYSQIFGVRWEAV
GTLDSSLATTSVEAGDGQVVYYLSDGKPVGVLLWNLPGRTDKAVTVLADPPEDLSTAIS
>Mature_458_residues
PCQGVYAWCGNPVSGGDGCHPSWQDMPLRADMPYEAWPSAKSSLEPSKRQGRQVTLVGVCIVSTMNPILGADMTTYQYLI
VGGGMAADSAARGIRDIDKKGSIAILSADVDAPYPRPALSKKLWTDPEFTWDQADLATVADTGAELRLGTEVLSIDRDGK
TVLTASGQVFGYQKLLLVTGLTPSRIDDDGDAVLYFRSARDYQQLRALAQPGHQFVVVGGGYIGAELAAGLVQQGCEVAL
VTPDPTLGGSQFPAQIASEYQKLFADAGVHLVTGKRVCSVRKHEAAEVTLDDGTILQADDVIAGLGASPVTKLAEEAGLT
VNDGVIVDEQLRTNDPAIWAAGDIANYPDPVLGRTRVEHVDNAIAMGKAAGRIMAGSKDSYTHTPMMYSQIFGVRWEAVG
TLDSSLATTSVEAGDGQVVYYLSDGKPVGVLLWNLPGRTDKAVTVLADPPEDLSTAIS

Specific function: The oxidation of camphor by cytochrome P450-CAM requires the participation of a flavoprotein, putidaredoxin reductase, and an iron-sulfur protein, putidaredoxin, to mediate the transfer of electrons from NADH to P450 for oxygen activation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4757732, Length=462, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI22202629, Length=462, Percent_Identity=28.5714285714286, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI65787454, Length=351, Percent_Identity=28.4900284900285, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI226437568, Length=351, Percent_Identity=28.4900284900285, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI21389617, Length=351, Percent_Identity=28.4900284900285, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI22202631, Length=277, Percent_Identity=27.7978339350181, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI195927004, Length=225, Percent_Identity=30.2222222222222, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI195927006, Length=186, Percent_Identity=31.1827956989247, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788892, Length=277, Percent_Identity=32.4909747292419, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789065, Length=285, Percent_Identity=29.8245614035088, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789765, Length=296, Percent_Identity=26.3513513513513, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI32564386, Length=419, Percent_Identity=28.4009546539379, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17559934, Length=399, Percent_Identity=25.5639097744361, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24581020, Length=466, Percent_Identity=28.5407725321888, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI28573993, Length=466, Percent_Identity=28.5407725321888, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI281359715, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI281359713, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639250, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI18543267, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639252, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24639257, Length=365, Percent_Identity=26.8493150684932, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585130, Length=366, Percent_Identity=24.5901639344262, Blast_Score=90, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 48584; Mature: 48453

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCQGVYAWCGNPVSGGDGCHPSWQDMPLRADMPYEAWPSAKSSLEPSKRQGRQVTLVGV
CCCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEE
CIVSTMNPILGADMTTYQYLIVGGGMAADSAARGIRDIDKKGSIAILSADVDAPYPRPAL
EEHHHCCHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
SKKLWTDPEFTWDQADLATVADTGAELRLGTEVLSIDRDGKTVLTASGQVFGYQKLLLVT
HHHCCCCCCCCCCCCCHHHHHCCCCCEEECCEEEEECCCCCEEEEECCCEECEEEEEEEE
GLTPSRIDDDGDAVLYFRSARDYQQLRALAQPGHQFVVVGGGYIGAELAAGLVQQGCEVA
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEE
LVTPDPTLGGSQFPAQIASEYQKLFADAGVHLVTGKRVCSVRKHEAAEVTLDDGTILQAD
EECCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCEEEEECCCCEEECC
DVIAGLGASPVTKLAEEAGLTVNDGVIVDEQLRTNDPAIWAAGDIANYPDPVLGRTRVEH
CHHCCCCCCHHHHHHHHCCCEECCCEEEECHHCCCCCCEEEECCCCCCCCCCCCCHHHHH
VDNAIAMGKAAGRIMAGSKDSYTHTPMMYSQIFGVRWEAVGTLDSSLATTSVEAGDGQVV
HHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHEEEEECCCCEEE
YYLSDGKPVGVLLWNLPGRTDKAVTVLADPPEDLSTAIS
EEECCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHCC
>Mature Secondary Structure 
PCQGVYAWCGNPVSGGDGCHPSWQDMPLRADMPYEAWPSAKSSLEPSKRQGRQVTLVGV
CCCCEEHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEE
CIVSTMNPILGADMTTYQYLIVGGGMAADSAARGIRDIDKKGSIAILSADVDAPYPRPAL
EEHHHCCHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
SKKLWTDPEFTWDQADLATVADTGAELRLGTEVLSIDRDGKTVLTASGQVFGYQKLLLVT
HHHCCCCCCCCCCCCCHHHHHCCCCCEEECCEEEEECCCCCEEEEECCCEECEEEEEEEE
GLTPSRIDDDGDAVLYFRSARDYQQLRALAQPGHQFVVVGGGYIGAELAAGLVQQGCEVA
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCEE
LVTPDPTLGGSQFPAQIASEYQKLFADAGVHLVTGKRVCSVRKHEAAEVTLDDGTILQAD
EECCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHCCCCEEEEECCCCEEECC
DVIAGLGASPVTKLAEEAGLTVNDGVIVDEQLRTNDPAIWAAGDIANYPDPVLGRTRVEH
CHHCCCCCCHHHHHHHHCCCEECCCEEEECHHCCCCCCEEEECCCCCCCCCCCCCHHHHH
VDNAIAMGKAAGRIMAGSKDSYTHTPMMYSQIFGVRWEAVGTLDSSLATTSVEAGDGQVV
HHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHCEEEEEECCCCCCHHEEEEECCCCEEE
YYLSDGKPVGVLLWNLPGRTDKAVTVLADPPEDLSTAIS
EEECCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180940; 2613690; 3003058; 12011076; 11524002 [H]