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The map label for this gene is gap [H]

Identifier: 50365395

GI number: 50365395

Start: 672923

End: 673936

Strand: Reverse

Name: gap [H]

Synonym: Mfl578

Alternate gene names: 50365395

Gene position: 673936-672923 (Counterclockwise)

Preceding gene: 50365396

Following gene: 50365394

Centisome position: 84.96

GC content: 33.04

Gene sequence:

>1014_bases
ATGTCAAAAAAAGTTGCAATTAACGGATTCGGAAGAATCGGACGTTTAACATTTAGACAATTATTCAACCAAGGAGTTGA
AATCGTTGCTGTTAACGATTTAACAGACACAAAAACATTAGCTTACTTATTAGAATTTGACTCAGCACAAGGAAAATTCC
AAGAAGGAAAAATTTCATACACAGATAACTCAATTATCGTTAATGGAAAAGAAATTAAAATCTTTGCTGAAAGAAATGCA
GCTGATTTACCTTGAGGTAAATTAGGAATCGATTTAGTAATCGAATCAACAGGATTCTACACAGATAAAGAAAAAGCATC
AGCACACTTAACAGCTGGAGCAAAAAAAGTTATTATTTCAGCACCTGCTACAGGAGAAATGAAAACAATCGTTTATGGTG
TAAACCACAAAAACTTATCAGCAGAAGACGTAATTATTTCAGGAGCTTCATGTACAACAAACTGTTTATCACCTGTTGCT
AAAATCATGGATGAAAAATTTGGAATCGTTAAAGGAAAAATGGTTACTGTTCATGCTGTAACAAATGACCAAAAATTATT
AGACTTACCTCATGGTGACTTAAGAAGAGGGCGTGCAGCAGCATGAAACATCGTTCCTTCAACAACTGGAGCAGCTAAAG
CAGTTTCATTAGTTTTACCAAACTTAAAAGGTAAATTAGATGGATACGCATTACGTGTTCCAACAATTACAGGATCAATT
ACAGACGTTACTTTACAATTAAGCAAAAAAACAACTGTTGAAGAAATCAACGCAACTGTTGAAGCAGCTATTAAAGCTGA
TGCTGAATTATCAAAAGCAATTAAATTTAATACACAACAAATCGTTTCAGGAGATACAATCGGATCATCATTCGGTTCAA
TCTTTGATGCAACATTAACAAAAATTACTGAAGTTGATGGTGAACAATTAGTTACTGTTTGTGCATGATATGACAATGAA
TCATCATACGTTTCACAATTAGTAAGAACAACAATTTACTTCATGGGATTATAA

Upstream 100 bases:

>100_bases
GAACATAATTTTTCAAAGATTTTTTTTGAAAAATTTGTTCTTTTTTATTTAAGAGTGTAAAATTATTAATGTATAGATAT
TACAAGAGAGGATAAAATAT

Downstream 100 bases:

>100_bases
TTCCTAGAATTAATAAATTTAAATACCCTTCGGGGTATTTTTTTTGTTATTAATTTAATAATTTATATAAAAGAGTTAAA
ATATATATTGTAAAGAAAAA

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSKKVAINGFGRIGRLTFRQLFNQGVEIVAVNDLTDTKTLAYLLEFDSAQGKFQEGKISYTDNSIIVNGKEIKIFAERNA
ADLPWGKLGIDLVIESTGFYTDKEKASAHLTAGAKKVIISAPATGEMKTIVYGVNHKNLSAEDVIISGASCTTNCLSPVA
KIMDEKFGIVKGKMVTVHAVTNDQKLLDLPHGDLRRGRAAAWNIVPSTTGAAKAVSLVLPNLKGKLDGYALRVPTITGSI
TDVTLQLSKKTTVEEINATVEAAIKADAELSKAIKFNTQQIVSGDTIGSSFGSIFDATLTKITEVDGEQLVTVCAWYDNE
SSYVSQLVRTTIYFMGL

Sequences:

>Translated_337_residues
MSKKVAINGFGRIGRLTFRQLFNQGVEIVAVNDLTDTKTLAYLLEFDSAQGKFQEGKISYTDNSIIVNGKEIKIFAERNA
ADLP*GKLGIDLVIESTGFYTDKEKASAHLTAGAKKVIISAPATGEMKTIVYGVNHKNLSAEDVIISGASCTTNCLSPVA
KIMDEKFGIVKGKMVTVHAVTNDQKLLDLPHGDLRRGRAAA*NIVPSTTGAAKAVSLVLPNLKGKLDGYALRVPTITGSI
TDVTLQLSKKTTVEEINATVEAAIKADAELSKAIKFNTQQIVSGDTIGSSFGSIFDATLTKITEVDGEQLVTVCA*YDNE
SSYVSQLVRTTIYFMGL
>Mature_336_residues
SKKVAINGFGRIGRLTFRQLFNQGVEIVAVNDLTDTKTLAYLLEFDSAQGKFQEGKISYTDNSIIVNGKEIKIFAERNAA
DLP*GKLGIDLVIESTGFYTDKEKASAHLTAGAKKVIISAPATGEMKTIVYGVNHKNLSAEDVIISGASCTTNCLSPVAK
IMDEKFGIVKGKMVTVHAVTNDQKLLDLPHGDLRRGRAAA*NIVPSTTGAAKAVSLVLPNLKGKLDGYALRVPTITGSIT
DVTLQLSKKTTVEEINATVEAAIKADAELSKAIKFNTQQIVSGDTIGSSFGSIFDATLTKITEVDGEQLVTVCA*YDNES
SYVSQLVRTTIYFMGL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=327, Percent_Identity=44.0366972477064, Blast_Score=251, Evalue=7e-67,
Organism=Homo sapiens, GI7657116, Length=333, Percent_Identity=39.6396396396396, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=45.2887537993921, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI1789295, Length=337, Percent_Identity=35.6083086053413, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=45.4005934718101, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=45.4005934718101, Blast_Score=248, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=43.9169139465875, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI32566163, Length=337, Percent_Identity=43.9169139465875, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6321631, Length=327, Percent_Identity=45.2599388379205, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322468, Length=327, Percent_Identity=45.2599388379205, Blast_Score=263, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6322409, Length=327, Percent_Identity=43.1192660550459, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=43.5045317220544, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=43.5045317220544, Blast_Score=251, Evalue=5e-67,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=42.1686746987952, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=42.1686746987952, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=42.2960725075529, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35663; Mature: 35532

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKVAINGFGRIGRLTFRQLFNQGVEIVAVNDLTDTKTLAYLLEFDSAQGKFQEGKISY
CCCEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHEEEEEEECCCCCCCCCCEEEE
TDNSIIVNGKEIKIFAERNAADLPGKLGIDLVIESTGFYTDKEKASAHLTAGAKKVIISA
CCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHCCEEEECCCEEEEEEC
PATGEMKTIVYGVNHKNLSAEDVIISGASCTTNCLSPVAKIMDEKFGIVKGKMVTVHAVT
CCCCCEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEEECEEEEEEEEC
NDQKLLDLPHGDLRRGRAAANIVPSTTGAAKAVSLVLPNLKGKLDGYALRVPTITGSITD
CCCEEEECCCCHHHCCCHHCCCCCCCCCHHHHHEEECCCCCCCCCCEEEEECEEECCEEE
VTLQLSKKTTVEEINATVEAAIKADAELSKAIKFNTQQIVSGDTIGSSFGSIFDATLTKI
EEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHH
TEVDGEQLVTVCAYDNESSYVSQLVRTTIYFMGL
HHCCCCEEEEEEEECCCHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SKKVAINGFGRIGRLTFRQLFNQGVEIVAVNDLTDTKTLAYLLEFDSAQGKFQEGKISY
CCEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHEEEEEEECCCCCCCCCCEEEE
TDNSIIVNGKEIKIFAERNAADLPGKLGIDLVIESTGFYTDKEKASAHLTAGAKKVIISA
CCCEEEEECCEEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHCCEEEECCCEEEEEEC
PATGEMKTIVYGVNHKNLSAEDVIISGASCTTNCLSPVAKIMDEKFGIVKGKMVTVHAVT
CCCCCEEEEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCEEECEEEEEEEEC
NDQKLLDLPHGDLRRGRAAANIVPSTTGAAKAVSLVLPNLKGKLDGYALRVPTITGSITD
CCCEEEECCCCHHHCCCHHCCCCCCCCCHHHHHEEECCCCCCCCCCEEEEECEEECCEEE
VTLQLSKKTTVEEINATVEAAIKADAELSKAIKFNTQQIVSGDTIGSSFGSIFDATLTKI
EEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHEECCCCCCCHHHHHHHHHHHHH
TEVDGEQLVTVCAYDNESSYVSQLVRTTIYFMGL
HHCCCCEEEEEEEECCCHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10463150; 11466286 [H]