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The map label for this gene is pgk [H]

Identifier: 50365394

GI number: 50365394

Start: 671596

End: 672810

Strand: Reverse

Name: pgk [H]

Synonym: Mfl577

Alternate gene names: 50365394

Gene position: 672810-671596 (Counterclockwise)

Preceding gene: 50365395

Following gene: 50365392

Centisome position: 84.82

GC content: 32.26

Gene sequence:

>1215_bases
ATGAATTACAATTTGAAAAAAACATTAAAAGATATTAATGTAAAAGGAAAAAAAGTTTTAGTTCGTGTTGACTTTAATGT
TCCATTAAAAGATGGAGTTATTACTGATGACAATCGTATTCAAGCAGCATTACCAACAATTAAATATTTAGTTGAAAATG
GCGCAAAAGTTATCTTATTTTCACACTTATCAAGAATTAAAAGTGAAGATGACAAAAAAACTAAATCATTAGCTCCAGTA
GCAAAAAGATTGGAAGAAGTTTTAGCACAATCAGTAAACTTTGTTAACCAAACAAGAGGAACTGAATTAGAAACTGCAAT
TAATGCATTAAATGAAAAAGAAGTTTTATTATTTGAAAACACTCGTTTCGAAGATGTAAAAGATGGTGAAGTAGTTAAAG
CTGAATCAAAAAATGATCCAGAATTAGGAAAATACTGAGCAAGCCTTGGAGATGTATTTATTAATGATGCATTTGGTACT
GCACACCGTGCTCATGCTTCAAACGTTGGTATAGCTTCAAACATTTCTGAATCAGCATTAGGTTTCTTAGTTCAAAATGA
AATTGAAATGTTAGGTAAAGGAATAGACGCACCTGAAAGACCTTTTGTTGCAATTTTAGGAGGAGCAAAAGTTTCTGACA
AAATTGGTGTTATTGATAACCTTTTAACTAAAGTTGACAAAATTTTAATTGGTGGAGGTATGACTTATACATTCCATAAA
GCAATGGGATTAGAAATTGGTAACTCATTATTAGAAGCTGATAAAGTTGAATTGGCTAAAGAATACTTAGAAAAAGCTAA
TGGAAAAATAATGTTACCTGTTGATAGTTTATGTGCTCCTGAATATGCTGATGTTGCTCCAACATTATGTGGAGAAAATG
TTCCAGCAGGAATGATGGGATTAGATATCGGACCAAAAACTATTGAAATGTATGAAAAAGAATTAGCAAACGCAAAAACT
GTTGTTTGAAATGGACCAATGGGAGTTAGTGAATTTGAAAACTTCAAAGCTGGTACTGTTGCTGTTTGTGAAGCTGCTGC
AAAACGTAAAGCTGATGGAGGTTTCACTTTAATTGGTGGAGGAGACTCAGCTGCAGCTGCAATTAAATTAGGATTCAAAG
ATGAATTCTCATGAATTTCTACAGGTGGTGGAGCTTCATTAGAATATATGGAAGGTAAAGAATTACCTGGAATTGCTGCA
ATCCAAAACAAATAA

Upstream 100 bases:

>100_bases
ATAAATTTAAATACCCTTCGGGGTATTTTTTTTGTTATTAATTTAATAATTTATATAAAAGAGTTAAAATATATATTGTA
AAGAAAAAGAGGAGAATAAA

Downstream 100 bases:

>100_bases
TTAATTAATATATAAAAAACAGACATGAGTCTGTTTTTCTTATACTCTAGATTGTTTAATTAAAATTAATAAAACCTTTA
ACGTTGAAGCAAAGAGCAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MNYNLKKTLKDINVKGKKVLVRVDFNVPLKDGVITDDNRIQAALPTIKYLVENGAKVILFSHLSRIKSEDDKKTKSLAPV
AKRLEEVLAQSVNFVNQTRGTELETAINALNEKEVLLFENTRFEDVKDGEVVKAESKNDPELGKYWASLGDVFINDAFGT
AHRAHASNVGIASNISESALGFLVQNEIEMLGKGIDAPERPFVAILGGAKVSDKIGVIDNLLTKVDKILIGGGMTYTFHK
AMGLEIGNSLLEADKVELAKEYLEKANGKIMLPVDSLCAPEYADVAPTLCGENVPAGMMGLDIGPKTIEMYEKELANAKT
VVWNGPMGVSEFENFKAGTVAVCEAAAKRKADGGFTLIGGGDSAAAAIKLGFKDEFSWISTGGGASLEYMEGKELPGIAA
IQNK

Sequences:

>Translated_404_residues
MNYNLKKTLKDINVKGKKVLVRVDFNVPLKDGVITDDNRIQAALPTIKYLVENGAKVILFSHLSRIKSEDDKKTKSLAPV
AKRLEEVLAQSVNFVNQTRGTELETAINALNEKEVLLFENTRFEDVKDGEVVKAESKNDPELGKY*ASLGDVFINDAFGT
AHRAHASNVGIASNISESALGFLVQNEIEMLGKGIDAPERPFVAILGGAKVSDKIGVIDNLLTKVDKILIGGGMTYTFHK
AMGLEIGNSLLEADKVELAKEYLEKANGKIMLPVDSLCAPEYADVAPTLCGENVPAGMMGLDIGPKTIEMYEKELANAKT
VV*NGPMGVSEFENFKAGTVAVCEAAAKRKADGGFTLIGGGDSAAAAIKLGFKDEFS*ISTGGGASLEYMEGKELPGIAA
IQNK
>Mature_404_residues
MNYNLKKTLKDINVKGKKVLVRVDFNVPLKDGVITDDNRIQAALPTIKYLVENGAKVILFSHLSRIKSEDDKKTKSLAPV
AKRLEEVLAQSVNFVNQTRGTELETAINALNEKEVLLFENTRFEDVKDGEVVKAESKNDPELGKY*ASLGDVFINDAFGT
AHRAHASNVGIASNISESALGFLVQNEIEMLGKGIDAPERPFVAILGGAKVSDKIGVIDNLLTKVDKILIGGGMTYTFHK
AMGLEIGNSLLEADKVELAKEYLEKANGKIMLPVDSLCAPEYADVAPTLCGENVPAGMMGLDIGPKTIEMYEKELANAKT
VV*NGPMGVSEFENFKAGTVAVCEAAAKRKADGGFTLIGGGDSAAAAIKLGFKDEFS*ISTGGGASLEYMEGKELPGIAA
IQNK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=418, Percent_Identity=44.7368421052632, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI31543397, Length=418, Percent_Identity=43.3014354066986, Blast_Score=304, Evalue=8e-83,
Organism=Escherichia coli, GI1789294, Length=399, Percent_Identity=42.6065162907268, Blast_Score=266, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17508823, Length=412, Percent_Identity=46.3592233009709, Blast_Score=323, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6319857, Length=419, Percent_Identity=47.0167064439141, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI17136394, Length=418, Percent_Identity=42.5837320574163, Blast_Score=295, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24581203, Length=414, Percent_Identity=42.7536231884058, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42830; Mature: 42830

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYNLKKTLKDINVKGKKVLVRVDFNVPLKDGVITDDNRIQAALPTIKYLVENGAKVILF
CCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEEH
SHLSRIKSEDDKKTKSLAPVAKRLEEVLAQSVNFVNQTRGTELETAINALNEKEVLLFEN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEC
TRFEDVKDGEVVKAESKNDPELGKYASLGDVFINDAFGTAHRAHASNVGIASNISESALG
CCCCCCCCCCEEEECCCCCCCCCCHHCCCHHEEECCCCCHHHHCCCCCCCCCCCCHHHHH
FLVQNEIEMLGKGIDAPERPFVAILGGAKVSDKIGVIDNLLTKVDKILIGGGMTYTFHKA
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEECCCCEEEHHHH
MGLEIGNSLLEADKVELAKEYLEKANGKIMLPVDSLCAPEYADVAPTLCGENVPAGMMGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCHHHCCHHHCCCCCCCCEEEC
DIGPKTIEMYEKELANAKTVVNGPMGVSEFENFKAGTVAVCEAAAKRKADGGFTLIGGGD
CCCCHHHHHHHHHHCCCCEEECCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCC
SAAAAIKLGFKDEFSISTGGGASLEYMEGKELPGIAAIQNK
CCEEEEEECCCCCCEEECCCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNYNLKKTLKDINVKGKKVLVRVDFNVPLKDGVITDDNRIQAALPTIKYLVENGAKVILF
CCCCHHHHHHHCCCCCEEEEEEEECCCCCCCCEECCCCCHHHHHHHHHHHHHCCCEEEEH
SHLSRIKSEDDKKTKSLAPVAKRLEEVLAQSVNFVNQTRGTELETAINALNEKEVLLFEN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEC
TRFEDVKDGEVVKAESKNDPELGKYASLGDVFINDAFGTAHRAHASNVGIASNISESALG
CCCCCCCCCCEEEECCCCCCCCCCHHCCCHHEEECCCCCHHHHCCCCCCCCCCCCHHHHH
FLVQNEIEMLGKGIDAPERPFVAILGGAKVSDKIGVIDNLLTKVDKILIGGGMTYTFHKA
HHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHEECCCCEEEHHHH
MGLEIGNSLLEADKVELAKEYLEKANGKIMLPVDSLCAPEYADVAPTLCGENVPAGMMGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCHHHCCHHHCCCCCCCCEEEC
DIGPKTIEMYEKELANAKTVVNGPMGVSEFENFKAGTVAVCEAAAKRKADGGFTLIGGGD
CCCCHHHHHHHHHHCCCCEEECCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCC
SAAAAIKLGFKDEFSISTGGGASLEYMEGKELPGIAAIQNK
CCEEEEEECCCCCCEEECCCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA