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The map label for this gene is dnaI [H]

Identifier: 50365396

GI number: 50365396

Start: 674029

End: 674964

Strand: Reverse

Name: dnaI [H]

Synonym: Mfl579

Alternate gene names: 50365396

Gene position: 674964-674029 (Counterclockwise)

Preceding gene: 50365397

Following gene: 50365395

Centisome position: 85.09

GC content: 24.89

Gene sequence:

>936_bases
ATGGATATTCAAATTTTTAAAGATAATAAAGTTATTGCAAATTTAATTGAAAATAGCAAAAATGAATCTCATGAAATAAC
AGATGAAGTTTTAAAGCAAAATTTTATTATTTTATTTGAATTTGTATCCGACTATACAGAATGTTCAACTGGTCCAATAA
AGGAATGTAAGCAAAGAATTAGGGGACGACAATTGAATTTAACTTTTAAAAATTCAAACTTTTATTTAAGTTCTTCACCG
TGTACTCATTTTCTATATGAAAATAAAAATCAAACAATTAAAAACAATTATATTTATAAAGACTTTAGTGTAGATGCATT
TCCTAAGACAGTAAAGAATTATTTAGAAGATTTAAAAACTGAAAACTTCTTTAGTGCTGAAGAAATTCAAGAGCGAGAAT
TGATAATAAAAAATTCGGGAAAATTAGTTGTTGATTATATTAAAGACATAAAAAATATTGATCGAACAAATATTAAGGGA
GTTTATTTACATGGTAATCCTGGTATTGGTAAAACAACAATGTTAAAAACATTAGCCAATGAAGCAGCAAAAAGAGATGC
CAAAGTATTCTTTTGCACAGCACCAGATATAATAGAAAAATGCAAGGAACAGTTTAGTAAAAACTCTAATTCTTCTATTC
CATATATTGATCAAATGAAAAAAGCTGACATTTTATTCATAGATGACTTTGCTGGTGAAATGGTTTCAAGCTGAACAAGA
GATAATTTATGATATATAATTTTGAATTACAGAATGAATAGAAAAAAACTCACTTTCATGTCATCAAATTTTACGTTTTA
TGAACTAGATAAAATTTATAATATAAATAAAAATAATAAAGAAATTGAAGTTATGAAAGTGGCTAGACTAAAAGAAAGAA
TAACAACTTTAACAACATTTGTAACACTAATGGGAAAACAATCTAAAAGAACATAA

Upstream 100 bases:

>100_bases
ACTGATAAAAATACTGAAGATCAAAATTATACATATGCTAACCAGCAAACAACAGAATTCGTAATGGAACAAAATATAGA
TATAGAATGGTAGGTCGAAT

Downstream 100 bases:

>100_bases
TTTTTCAAAGATTTTTTTTGAAAAATTTGTTCTTTTTTATTTAAGAGTGTAAAATTATTAATGTATAGATATTACAAGAG
AGGATAAAATATATGTCAAA

Product: chromosomal replication initiator protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MDIQIFKDNKVIANLIENSKNESHEITDEVLKQNFIILFEFVSDYTECSTGPIKECKQRIRGRQLNLTFKNSNFYLSSSP
CTHFLYENKNQTIKNNYIYKDFSVDAFPKTVKNYLEDLKTENFFSAEEIQERELIIKNSGKLVVDYIKDIKNIDRTNIKG
VYLHGNPGIGKTTMLKTLANEAAKRDAKVFFCTAPDIIEKCKEQFSKNSNSSIPYIDQMKKADILFIDDFAGEMVSSWTR
DNLWYIILNYRMNRKKLTFMSSNFTFYELDKIYNINKNNKEIEVMKVARLKERITTLTTFVTLMGKQSKRT

Sequences:

>Translated_311_residues
MDIQIFKDNKVIANLIENSKNESHEITDEVLKQNFIILFEFVSDYTECSTGPIKECKQRIRGRQLNLTFKNSNFYLSSSP
CTHFLYENKNQTIKNNYIYKDFSVDAFPKTVKNYLEDLKTENFFSAEEIQERELIIKNSGKLVVDYIKDIKNIDRTNIKG
VYLHGNPGIGKTTMLKTLANEAAKRDAKVFFCTAPDIIEKCKEQFSKNSNSSIPYIDQMKKADILFIDDFAGEMVSS*TR
DNL*YIILNYRMNRKKLTFMSSNFTFYELDKIYNINKNNKEIEVMKVARLKERITTLTTFVTLMGKQSKRT
>Mature_311_residues
MDIQIFKDNKVIANLIENSKNESHEITDEVLKQNFIILFEFVSDYTECSTGPIKECKQRIRGRQLNLTFKNSNFYLSSSP
CTHFLYENKNQTIKNNYIYKDFSVDAFPKTVKNYLEDLKTENFFSAEEIQERELIIKNSGKLVVDYIKDIKNIDRTNIKG
VYLHGNPGIGKTTMLKTLANEAAKRDAKVFFCTAPDIIEKCKEQFSKNSNSSIPYIDQMKKADILFIDDFAGEMVSS*TR
DNL*YIILNYRMNRKKLTFMSSNFTFYELDKIYNINKNNKEIEVMKVARLKERITTLTTFVTLMGKQSKRT

Specific function: Probably involved in DNA replication [H]

COG id: COG1484

COG function: function code L; DNA replication protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR009928
- InterPro:   IPR002611 [H]

Pfam domain/function: PF07319 DnaI_N; PF01695 IstB [H]

EC number: NA

Molecular weight: Translated: 36017; Mature: 36017

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIQIFKDNKVIANLIENSKNESHEITDEVLKQNFIILFEFVSDYTECSTGPIKECKQRI
CCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCCHHHHHHHH
RGRQLNLTFKNSNFYLSSSPCTHFLYENKNQTIKNNYIYKDFSVDAFPKTVKNYLEDLKT
CCCEEEEEEECCEEEEECCCCEEEEECCCCCEEECCEEEEECCCCCCHHHHHHHHHHHCC
ENFFSAEEIQERELIIKNSGKLVVDYIKDIKNIDRTNIKGVYLHGNPGIGKTTMLKTLAN
CCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHH
EAAKRDAKVFFCTAPDIIEKCKEQFSKNSNSSIPYIDQMKKADILFIDDFAGEMVSSTRD
HHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEECHHHHHHHCCCC
NLYIILNYRMNRKKLTFMSSNFTFYELDKIYNINKNNKEIEVMKVARLKERITTLTTFVT
CEEEEEEEECCCEEEEEEECCCEEEEECCEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
LMGKQSKRT
HHCCCCCCC
>Mature Secondary Structure
MDIQIFKDNKVIANLIENSKNESHEITDEVLKQNFIILFEFVSDYTECSTGPIKECKQRI
CCEEEECCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEHHHHHHHHCCCCCHHHHHHHH
RGRQLNLTFKNSNFYLSSSPCTHFLYENKNQTIKNNYIYKDFSVDAFPKTVKNYLEDLKT
CCCEEEEEEECCEEEEECCCCEEEEECCCCCEEECCEEEEECCCCCCHHHHHHHHHHHCC
ENFFSAEEIQERELIIKNSGKLVVDYIKDIKNIDRTNIKGVYLHGNPGIGKTTMLKTLAN
CCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHH
EAAKRDAKVFFCTAPDIIEKCKEQFSKNSNSSIPYIDQMKKADILFIDDFAGEMVSSTRD
HHHCCCCEEEEEECHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEECHHHHHHHCCCC
NLYIILNYRMNRKKLTFMSSNFTFYELDKIYNINKNNKEIEVMKVARLKERITTLTTFVT
CEEEEEEEECCCEEEEEEECCCEEEEECCEEECCCCCCEEHHHHHHHHHHHHHHHHHHHH
LMGKQSKRT
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027671; 8969504; 9387221; 9384377; 3027697; 1549481; 7773414 [H]