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The map label for this gene is greA

Identifier: 50364917

GI number: 50364917

Start: 129303

End: 129773

Strand: Direct

Name: greA

Synonym: Mfl102

Alternate gene names: 50364917

Gene position: 129303-129773 (Clockwise)

Preceding gene: 50364916

Following gene: 50364918

Centisome position: 16.3

GC content: 30.15

Gene sequence:

>471_bases
ATGGCAAAAGAAATAATCTTAACTCAAGAAGGTATTGAGGAATTAAAACACGAATTAAAACACTTATTAGAAGTTGTTCG
TCCACAAGTAATTGAAGAATTAGTAGAAGCACGTAATCAAGGAGATTTAAGTGAAAATGCTGATTATGATGCAGCTCGTA
ATCGTCAAGCTGAAGTTGAAGCAAGAATAAAAGAACTTGAAACAATGATAAGCAAAGCAAAATTAATTGAAGATTCTTCT
ACAACTGATGGAGCAATTAAAATTGGTTCAAAAGTTAAATTCATCATGTTAAATACTAAACAAACAAGAGAAGTTAAAAT
TGTAGGAGCTGTTGAAGCTGATCCATTTAAAGGTTTAATTTCAAATGAATCACCAATAGCTAAAGCAATTTTAGGAAAAA
AAGTTGGGGAATCAGTTGAAGTTAAAGATATCAATGCGCCTTACTCAATTGAAATCAAAGAAGTTAATTAA

Upstream 100 bases:

>100_bases
GCTTTAGACAACCTGAACATATATTTAAAGAACAGAAAATAGATACAAATTTAAGAACAAATAATATATAATTAAAAAGT
TAATGAATTAGGAGACAAAC

Downstream 100 bases:

>100_bases
TAATTACCCTTTTGGGTAATTTTATTTTAAGGAGAGTTATGAGAAATCAATCAACATTTAAAAATGATAAACCTACAATA
TATTTAGTTGGTACACCAAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MAKEIILTQEGIEELKHELKHLLEVVRPQVIEELVEARNQGDLSENADYDAARNRQAEVEARIKELETMISKAKLIEDSS
TTDGAIKIGSKVKFIMLNTKQTREVKIVGAVEADPFKGLISNESPIAKAILGKKVGESVEVKDINAPYSIEIKEVN

Sequences:

>Translated_156_residues
MAKEIILTQEGIEELKHELKHLLEVVRPQVIEELVEARNQGDLSENADYDAARNRQAEVEARIKELETMISKAKLIEDSS
TTDGAIKIGSKVKFIMLNTKQTREVKIVGAVEADPFKGLISNESPIAKAILGKKVGESVEVKDINAPYSIEIKEVN
>Mature_155_residues
AKEIILTQEGIEELKHELKHLLEVVRPQVIEELVEARNQGDLSENADYDAARNRQAEVEARIKELETMISKAKLIEDSST
TDGAIKIGSKVKFIMLNTKQTREVKIVGAVEADPFKGLISNESPIAKAILGKKVGESVEVKDINAPYSIEIKEVN

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=139, Percent_Identity=39.568345323741, Blast_Score=95, Evalue=2e-21,
Organism=Escherichia coli, GI87082261, Length=135, Percent_Identity=35.5555555555556, Blast_Score=81, Evalue=4e-17,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_MESFL (Q6F215)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053342.1
- HSSP:   P0A6W5
- ProteinModelPortal:   Q6F215
- SMR:   Q6F215
- GeneID:   2897868
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl102
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   MFLO265311:MFL102-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17309; Mature: 17178

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEIILTQEGIEELKHELKHLLEVVRPQVIEELVEARNQGDLSENADYDAARNRQAEVE
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHHH
ARIKELETMISKAKLIEDSSTTDGAIKIGSKVKFIMLNTKQTREVKIVGAVEADPFKGLI
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEECCCCCCEEEEEEECCCCCCCCCC
SNESPIAKAILGKKVGESVEVKDINAPYSIEIKEVN
CCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEECC
>Mature Secondary Structure 
AKEIILTQEGIEELKHELKHLLEVVRPQVIEELVEARNQGDLSENADYDAARNRQAEVE
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCHHHHH
ARIKELETMISKAKLIEDSSTTDGAIKIGSKVKFIMLNTKQTREVKIVGAVEADPFKGLI
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEEEEEECCCCCCEEEEEEECCCCCCCCCC
SNESPIAKAILGKKVGESVEVKDINAPYSIEIKEVN
CCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA