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The map label for this gene is pepA [H]

Identifier: 50364886

GI number: 50364886

Start: 91753

End: 93096

Strand: Direct

Name: pepA [H]

Synonym: Mfl071

Alternate gene names: 50364886

Gene position: 91753-93096 (Clockwise)

Preceding gene: 50364885

Following gene: 50364887

Centisome position: 11.57

GC content: 30.51

Gene sequence:

>1344_bases
ATGATTACATTAAACAAAAAAAATCATGATATTAAATTAGTTGCTGTTGCTGACAACAAAGCTAATGAATTTATCAAAGA
CAGTGCGGGAGCAGTTACATTTATTTCAGAAGATAAAACAATTTATTTAGTTATTAAACAAAAAGGATGTAGAGTTAAAC
AAATTAAAGCGGGATTAAAATCAGCTTTATCAACTTACACAAAAAATTTAAATATTGACTTAGACTCAATTATTAATCAA
TTAGGAGAAGAAAATAAAGATTTAGTTTTTAACACAGTTTATGAAACAATTTCATTCTTATCACATGATGTAGTTTCATT
CAAAAAAGAATCAAAACCAAGCAAAATTGAATTCAATATTGAAACTAAAAATGACTTTTCTGAATTAGAAGCTGCTGCAG
CTATTAAAACAGAATTTATTAATTATGCTAGAGATTTACAAGATACACCTCCAAATATTGGAACAAGTGAATACTATGCT
GAAAAAGTATCAAAAGATGCAGCAAACATTGAAGGATTAAAAGTTACTGTTCTTGGAAGAAAAGAAGCAGAAAAATTCGG
AATGGGATTATTCTTAGGAGTTAATGCTGGATCAGCTCATGAACCAAGAATTGTTGTTATGGAATACTGTGGAGATTCTT
CAAAACCAAAAACAGGTTTAGTTGGAAAAGGAATTTGTTTTGATTCTGGAGGGTACAACTTAAAACCTTCAGCTTCAATG
TTAGGTATGAAATTTGATATGTCTGGAGCAGCTATTATGTTATCTGCTACTATGGCATTAGCTAAAGCAAAAGCAAAAGC
AAATGTAGTCGCTGTAGCAATGTTCACTGATAATAAAATTGGACAAAATGCAACTTTACCTGAATCAGTTTTAACATCAA
TGAATGGTTTAACTGTAGAAATCAATAATACTGATGCTGAAGGAAGATTAGTTTTAGCGGATGGTATGACATATGCTATT
AGAGAAAAAGGCGTAGAACAAATTATTGAGGCTTCAACTTTAACTGGAGCATTAGTAGTTGCTTTAGGAAGTTCAGCTAT
TGGTGTATTTACTCATCAAGACGAATTATGAAATACTTTTGAAAAAGCTTCATTAAAAACTAGAGAAAGAATGTGAAGAT
TACCAATCTTTGAAGAGCATTTAGAAAGAGTTAAATCTGGAGCAGTTTTAGGTGACTTATCAAATGCTGTTAAAGGATAT
GAAGGAAGTTCAACTGCAGCAGCATTCTTAAATGAATTTGCTGAAGAAAAACCATTTATTCACTTTGATATTGCTGGAAC
AGCAGATATTGATGGACGTGGACAAGGTGTTTTAGTTAAAACATTATTTGAAGTTCTAAAATAA

Upstream 100 bases:

>100_bases
TTGAAATAAAGTGGAATTCCACTTTTTTCTTTTATTAAGTACTTATTGTTTTAGGTATTAAGTGCTATAATTTTTTTAAA
TAGAAAGATGGTATATATTT

Downstream 100 bases:

>100_bases
TAAAATTAATCATAAGTATTCACTTATGATTTTTTTAAATAGAAAAGAGATTATATGATAACAATAAATACAAATAAATT
AGAATATAAATTAATTGCTG

Product: cytosol aminopeptidase (leucine aminopeptidase)

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MITLNKKNHDIKLVAVADNKANEFIKDSAGAVTFISEDKTIYLVIKQKGCRVKQIKAGLKSALSTYTKNLNIDLDSIINQ
LGEENKDLVFNTVYETISFLSHDVVSFKKESKPSKIEFNIETKNDFSELEAAAAIKTEFINYARDLQDTPPNIGTSEYYA
EKVSKDAANIEGLKVTVLGRKEAEKFGMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSASM
LGMKFDMSGAAIMLSATMALAKAKAKANVVAVAMFTDNKIGQNATLPESVLTSMNGLTVEINNTDAEGRLVLADGMTYAI
REKGVEQIIEASTLTGALVVALGSSAIGVFTHQDELWNTFEKASLKTRERMWRLPIFEEHLERVKSGAVLGDLSNAVKGY
EGSSTAAAFLNEFAEEKPFIHFDIAGTADIDGRGQGVLVKTLFEVLK

Sequences:

>Translated_447_residues
MITLNKKNHDIKLVAVADNKANEFIKDSAGAVTFISEDKTIYLVIKQKGCRVKQIKAGLKSALSTYTKNLNIDLDSIINQ
LGEENKDLVFNTVYETISFLSHDVVSFKKESKPSKIEFNIETKNDFSELEAAAAIKTEFINYARDLQDTPPNIGTSEYYA
EKVSKDAANIEGLKVTVLGRKEAEKFGMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSASM
LGMKFDMSGAAIMLSATMALAKAKAKANVVAVAMFTDNKIGQNATLPESVLTSMNGLTVEINNTDAEGRLVLADGMTYAI
REKGVEQIIEASTLTGALVVALGSSAIGVFTHQDEL*NTFEKASLKTRERM*RLPIFEEHLERVKSGAVLGDLSNAVKGY
EGSSTAAAFLNEFAEEKPFIHFDIAGTADIDGRGQGVLVKTLFEVLK
>Mature_447_residues
MITLNKKNHDIKLVAVADNKANEFIKDSAGAVTFISEDKTIYLVIKQKGCRVKQIKAGLKSALSTYTKNLNIDLDSIINQ
LGEENKDLVFNTVYETISFLSHDVVSFKKESKPSKIEFNIETKNDFSELEAAAAIKTEFINYARDLQDTPPNIGTSEYYA
EKVSKDAANIEGLKVTVLGRKEAEKFGMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSASM
LGMKFDMSGAAIMLSATMALAKAKAKANVVAVAMFTDNKIGQNATLPESVLTSMNGLTVEINNTDAEGRLVLADGMTYAI
REKGVEQIIEASTLTGALVVALGSSAIGVFTHQDEL*NTFEKASLKTRERM*RLPIFEEHLERVKSGAVLGDLSNAVKGY
EGSSTAAAFLNEFAEEKPFIHFDIAGTADIDGRGQGVLVKTLFEVLK

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=291, Percent_Identity=40.5498281786942, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI47155554, Length=356, Percent_Identity=31.1797752808989, Blast_Score=152, Evalue=8e-37,
Organism=Escherichia coli, GI1790710, Length=329, Percent_Identity=37.3860182370821, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87082123, Length=301, Percent_Identity=37.2093023255814, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17556903, Length=305, Percent_Identity=33.1147540983607, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17565172, Length=215, Percent_Identity=32.093023255814, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355725, Length=289, Percent_Identity=32.8719723183391, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24661038, Length=310, Percent_Identity=31.9354838709677, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI221379063, Length=317, Percent_Identity=32.807570977918, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI221379062, Length=317, Percent_Identity=32.807570977918, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21357381, Length=317, Percent_Identity=32.807570977918, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI20129969, Length=293, Percent_Identity=29.6928327645051, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19922386, Length=294, Percent_Identity=32.6530612244898, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355645, Length=322, Percent_Identity=28.8819875776398, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662223, Length=322, Percent_Identity=28.8819875776398, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24662227, Length=320, Percent_Identity=28.125, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161077148, Length=293, Percent_Identity=30.0341296928328, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20130057, Length=293, Percent_Identity=30.0341296928328, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129963, Length=290, Percent_Identity=30.6896551724138, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646701, Length=288, Percent_Identity=30.2083333333333, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24646703, Length=288, Percent_Identity=30.2083333333333, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21358201, Length=288, Percent_Identity=30.2083333333333, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 48080; Mature: 48080

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLNKKNHDIKLVAVADNKANEFIKDSAGAVTFISEDKTIYLVIKQKGCRVKQIKAGLK
CEEECCCCCCEEEEEEECCCCHHHHHCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHH
SALSTYTKNLNIDLDSIINQLGEENKDLVFNTVYETISFLSHDVVSFKKESKPSKIEFNI
HHHHHHHCCCCEEHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ETKNDFSELEAAAAIKTEFINYARDLQDTPPNIGTSEYYAEKVSKDAANIEGLKVTVLGR
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEC
KEAEKFGMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSASM
HHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHH
LGMKFDMSGAAIMLSATMALAKAKAKANVVAVAMFTDNKIGQNATLPESVLTSMNGLTVE
EEEEECCCCCEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCEEEE
INNTDAEGRLVLADGMTYAIREKGVEQIIEASTLTGALVVALGSSAIGVFTHQDELNTFE
EECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECHHHHHHH
KASLKTRERMRLPIFEEHLERVKSGAVLGDLSNAVKGYEGSSTAAAFLNEFAEEKPFIHF
HHHHHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHCCCCCCHHHHHHHHHHHHCCCEEEE
DIAGTADIDGRGQGVLVKTLFEVLK
EECCCCCCCCCCCEEHHHHHHHHHC
>Mature Secondary Structure
MITLNKKNHDIKLVAVADNKANEFIKDSAGAVTFISEDKTIYLVIKQKGCRVKQIKAGLK
CEEECCCCCCEEEEEEECCCCHHHHHCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHH
SALSTYTKNLNIDLDSIINQLGEENKDLVFNTVYETISFLSHDVVSFKKESKPSKIEFNI
HHHHHHHCCCCEEHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ETKNDFSELEAAAAIKTEFINYARDLQDTPPNIGTSEYYAEKVSKDAANIEGLKVTVLGR
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEEC
KEAEKFGMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSASM
HHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHH
LGMKFDMSGAAIMLSATMALAKAKAKANVVAVAMFTDNKIGQNATLPESVLTSMNGLTVE
EEEEECCCCCEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHCCCEEEE
INNTDAEGRLVLADGMTYAIREKGVEQIIEASTLTGALVVALGSSAIGVFTHQDELNTFE
EECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCEEEEEEECHHHHHHH
KASLKTRERMRLPIFEEHLERVKSGAVLGDLSNAVKGYEGSSTAAAFLNEFAEEKPFIHF
HHHHHHHHHCCCCHHHHHHHHHHCCCEEECHHHHHCCCCCCHHHHHHHHHHHHCCCEEEE
DIAGTADIDGRGQGVLVKTLFEVLK
EECCCCCCCCCCCEEHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7557291 [H]