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The map label for this gene is pepA [H]

Identifier: 50364887

GI number: 50364887

Start: 93151

End: 94500

Strand: Direct

Name: pepA [H]

Synonym: Mfl072

Alternate gene names: 50364887

Gene position: 93151-94500 (Clockwise)

Preceding gene: 50364886

Following gene: 50364888

Centisome position: 11.74

GC content: 27.93

Gene sequence:

>1350_bases
ATGATAACAATAAATACAAATAAATTAGAATATAAATTAATTGCTGTGCCCAACAATGAAACAAATATTAATATTCTTGA
TAAACCAAGTTGTGTAACCTTTATTAGTGAAAGTAAAACCTTCTATCTTGTTATTAAAAAAGATTCAATGTCATATTTAA
GACAATTAAAATTAGGTTTAGCAAGTTTTATTCAAATGTGTAAAGGTGATACTAATATTGATTTTAATAGTTTTGTTAAA
GTTATACCAAACGTAAATAAAGAAATAATATTTAATGTAATTTGTGATGTTATTTTGTTTGAAACACACAAATTTATAAC
ATATAAAAAAGATGTAAAGACAAATGACTATTTGTTTAATATAGATCAGTCTGAAGATTTTTCTGACCTATTAGATGAGA
ATATTTTAAAAAATAAATATGTTAATTATGCTAGAGATTTACAAGATTTTCCACCAAATATTGGAACTAGTGATTTCTAT
GGTGAAAAGCTTCTAAAAGATTCTTCAAAAATTGAGGGTTTAAAAGTTAGTGTATTAAAAAATAAAGAGTTAAAAGAATT
AGATATGAATTTAGTCTTAGCAGTAAACCAAGGTTCAAATCATGATGCAAATGTTGTAGTTATGGAATTTAACAATAATC
CTGGTGGCAAAAAAATAGCCCTTGTAGGTAAAGGGATTTGCTTTGATACTGGAGGTTATGATTTGAAACCATCAGCTTTT
ATTGAAGGAATGAAATTTGATATGACTGGTGCGGCAATAGTTATGTCAATTGCAATGGCATTGGCAGAGGCAAAAGCTAA
AATTAATTTGGTTGCTGTAGGATTATTTACAGATAATAAAATTGGACAAAAAGCAGTACTACCAGAATCAATAATAAAAT
CTAAAAATGGTTTAACTGTTGAAATTAGCAATACTGATGCTGAAGGAAGATTGGTTCTAGCAGATGGTATAACATATGCT
ATTAGAGAAAAAAATGCAGATGTTATTTATGACATTGCAACTTTAACTGGTGCTACTGCAATGGCACTTGGAGAATCTGC
TTCAGGAGTTTTTACAGAATTTGATGAAATGTGAAAAAAATTAAATGAAGCATCAAACTATACAACAGAAAGATTTTGAA
GATTACCAATTTTTGCTGAACATAAAGAACAAGTTAAGAATGATTCAGTTTTAGCCGATCTAACTAATTCATGCAAAAAA
GGGCCAGGAGGTTCAACTGCAGCAGCATTTTTAACTTTCTTTGCAGAAGATAAAGAGTTTCTTCACATTGATTGTTCAAG
AGTTGTAAGAGTAGGATTAAAGGGACAAGGTTATATTGTTAAAACAATGTTTGAATTACTTAAAAAATAA

Upstream 100 bases:

>100_bases
TGGACAAGGTGTTTTAGTTAAAACATTATTTGAAGTTCTAAAATAATAAAATTAATCATAAGTATTCACTTATGATTTTT
TTAAATAGAAAAGAGATTAT

Downstream 100 bases:

>100_bases
TTAATGCATTTTTAATATATAATATGAATTAAGAAAATAGAGGAACAAATATGAAAATTAATACATGAACATTTTATGAC
GCTAAAGATTTAGTTGATGT

Product: cytosol aminopeptidase (leucine aminopeptidase)

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MITINTNKLEYKLIAVPNNETNINILDKPSCVTFISESKTFYLVIKKDSMSYLRQLKLGLASFIQMCKGDTNIDFNSFVK
VIPNVNKEIIFNVICDVILFETHKFITYKKDVKTNDYLFNIDQSEDFSDLLDENILKNKYVNYARDLQDFPPNIGTSDFY
GEKLLKDSSKIEGLKVSVLKNKELKELDMNLVLAVNQGSNHDANVVVMEFNNNPGGKKIALVGKGICFDTGGYDLKPSAF
IEGMKFDMTGAAIVMSIAMALAEAKAKINLVAVGLFTDNKIGQKAVLPESIIKSKNGLTVEISNTDAEGRLVLADGITYA
IREKNADVIYDIATLTGATAMALGESASGVFTEFDEMWKKLNEASNYTTERFWRLPIFAEHKEQVKNDSVLADLTNSCKK
GPGGSTAAAFLTFFAEDKEFLHIDCSRVVRVGLKGQGYIVKTMFELLKK

Sequences:

>Translated_449_residues
MITINTNKLEYKLIAVPNNETNINILDKPSCVTFISESKTFYLVIKKDSMSYLRQLKLGLASFIQMCKGDTNIDFNSFVK
VIPNVNKEIIFNVICDVILFETHKFITYKKDVKTNDYLFNIDQSEDFSDLLDENILKNKYVNYARDLQDFPPNIGTSDFY
GEKLLKDSSKIEGLKVSVLKNKELKELDMNLVLAVNQGSNHDANVVVMEFNNNPGGKKIALVGKGICFDTGGYDLKPSAF
IEGMKFDMTGAAIVMSIAMALAEAKAKINLVAVGLFTDNKIGQKAVLPESIIKSKNGLTVEISNTDAEGRLVLADGITYA
IREKNADVIYDIATLTGATAMALGESASGVFTEFDEM*KKLNEASNYTTERF*RLPIFAEHKEQVKNDSVLADLTNSCKK
GPGGSTAAAFLTFFAEDKEFLHIDCSRVVRVGLKGQGYIVKTMFELLKK
>Mature_449_residues
MITINTNKLEYKLIAVPNNETNINILDKPSCVTFISESKTFYLVIKKDSMSYLRQLKLGLASFIQMCKGDTNIDFNSFVK
VIPNVNKEIIFNVICDVILFETHKFITYKKDVKTNDYLFNIDQSEDFSDLLDENILKNKYVNYARDLQDFPPNIGTSDFY
GEKLLKDSSKIEGLKVSVLKNKELKELDMNLVLAVNQGSNHDANVVVMEFNNNPGGKKIALVGKGICFDTGGYDLKPSAF
IEGMKFDMTGAAIVMSIAMALAEAKAKINLVAVGLFTDNKIGQKAVLPESIIKSKNGLTVEISNTDAEGRLVLADGITYA
IREKNADVIYDIATLTGATAMALGESASGVFTEFDEM*KKLNEASNYTTERF*RLPIFAEHKEQVKNDSVLADLTNSCKK
GPGGSTAAAFLTFFAEDKEFLHIDCSRVVRVGLKGQGYIVKTMFELLKK

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=322, Percent_Identity=36.3354037267081, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI47155554, Length=313, Percent_Identity=31.3099041533546, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1790710, Length=284, Percent_Identity=37.6760563380282, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI87082123, Length=327, Percent_Identity=36.085626911315, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17556903, Length=308, Percent_Identity=32.7922077922078, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17565172, Length=186, Percent_Identity=34.9462365591398, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357381, Length=332, Percent_Identity=31.3253012048193, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221379063, Length=332, Percent_Identity=31.3253012048193, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI221379062, Length=332, Percent_Identity=31.3253012048193, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355725, Length=357, Percent_Identity=28.5714285714286, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24661038, Length=320, Percent_Identity=29.375, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161077148, Length=298, Percent_Identity=26.8456375838926, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20130057, Length=298, Percent_Identity=26.8456375838926, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI20129969, Length=308, Percent_Identity=24.6753246753247, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24646701, Length=258, Percent_Identity=28.6821705426357, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24646703, Length=258, Percent_Identity=28.6821705426357, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358201, Length=258, Percent_Identity=28.6821705426357, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24662227, Length=305, Percent_Identity=24.5901639344262, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355645, Length=293, Percent_Identity=24.5733788395904, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24662223, Length=293, Percent_Identity=24.5733788395904, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI20129963, Length=298, Percent_Identity=28.1879194630872, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19922386, Length=300, Percent_Identity=24.3333333333333, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 49525; Mature: 49525

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITINTNKLEYKLIAVPNNETNINILDKPSCVTFISESKTFYLVIKKDSMSYLRQLKLGL
CEEEECCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHH
ASFIQMCKGDTNIDFNSFVKVIPNVNKEIIFNVICDVILFETHKFITYKKDVKTNDYLFN
HHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEE
IDQSEDFSDLLDENILKNKYVNYARDLQDFPPNIGTSDFYGEKLLKDSSKIEGLKVSVLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEC
NKELKELDMNLVLAVNQGSNHDANVVVMEFNNNPGGKKIALVGKGICFDTGGYDLKPSAF
CCCHHHCCCEEEEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCEEEECCCCCCCHHHH
IEGMKFDMTGAAIVMSIAMALAEAKAKINLVAVGLFTDNKIGQKAVLPESIIKSKNGLTV
HCCCEECCCHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCHHHHCCCCCEEE
EISNTDAEGRLVLADGITYAIREKNADVIYDIATLTGATAMALGESASGVFTEFDEMKKL
EEECCCCCCEEEEECCCEEEEECCCCCEEEEEHHHCCCHHHHCCCCCCCHHHHHHHHHHH
NEASNYTTERFRLPIFAEHKEQVKNDSVLADLTNSCKKGPGGSTAAAFLTFFAEDKEFLH
HHHCCCCHHHEECCEEECHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
IDCSRVVRVGLKGQGYIVKTMFELLKK
EEHHHHHEECCCCCCEEHHHHHHHHCC
>Mature Secondary Structure
MITINTNKLEYKLIAVPNNETNINILDKPSCVTFISESKTFYLVIKKDSMSYLRQLKLGL
CEEEECCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHH
ASFIQMCKGDTNIDFNSFVKVIPNVNKEIIFNVICDVILFETHKFITYKKDVKTNDYLFN
HHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEE
IDQSEDFSDLLDENILKNKYVNYARDLQDFPPNIGTSDFYGEKLLKDSSKIEGLKVSVLK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCEEEEEEC
NKELKELDMNLVLAVNQGSNHDANVVVMEFNNNPGGKKIALVGKGICFDTGGYDLKPSAF
CCCHHHCCCEEEEEEECCCCCCCEEEEEEECCCCCCCEEEEEECCEEEECCCCCCCHHHH
IEGMKFDMTGAAIVMSIAMALAEAKAKINLVAVGLFTDNKIGQKAVLPESIIKSKNGLTV
HCCCEECCCHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCCCCCCCHHHHCCCCCEEE
EISNTDAEGRLVLADGITYAIREKNADVIYDIATLTGATAMALGESASGVFTEFDEMKKL
EEECCCCCCEEEEECCCEEEEECCCCCEEEEEHHHCCCHHHHCCCCCCCHHHHHHHHHHH
NEASNYTTERFRLPIFAEHKEQVKNDSVLADLTNSCKKGPGGSTAAAFLTFFAEDKEFLH
HHHCCCCHHHEECCEEECHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
IDCSRVVRVGLKGQGYIVKTMFELLKK
EEHHHHHEECCCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]