Click here to switch to the map view.

The map label for this gene is pepA [H]

Identifier: 50364885

GI number: 50364885

Start: 90315

End: 91661

Strand: Direct

Name: pepA [H]

Synonym: Mfl070

Alternate gene names: 50364885

Gene position: 90315-91661 (Clockwise)

Preceding gene: 50364884

Following gene: 50364886

Centisome position: 11.39

GC content: 28.51

Gene sequence:

>1347_bases
ATGATTCAAATAAATGAAACTAAAAATACAATTAAAATAGTTGGAGTTAAAGATAACTCAAAAAATACTTTTATAAAAAA
TAAAGTTGGAGTACCAACTTTAATATCTGAAGATAAAACAATTTATATTGTTTTCAAAAAAGACGAAGAAACTTTTACAG
AACAAATTAAAAAAGGAATAGAAGCAACAATATCAAATTTTGATTTTGATGTAGATATTGACATTGATTCTTTTGAAAAT
GTTTGTGATGGAATTAGTAAAGAAGAAATTATTAAAACAGTTTATGAAACAATATCTTTTTCAACACATAAAGGTTTATC
AATTGAAAAAGTAGAAAAAGAAACAAAATATAATTTAATTTTTACTGGTAAACATCAGGAATTAATAAATCAATTAACTA
TTGTTTGTGAATATATCAATTATGCTAGAGATTTACAAGACACACCTCCAAATATTGGAACAAGTGAATACTATGCTGAC
AAACTTGTAAGTGATGCAAAAGATATAAAAGGTTTAAAGGTTACTATTTTGGGTAGAAAAGAAGCAACTAAATTAAACAT
GGGCTTATTTTTAGGAGTTAATGCTGGATCAGCTCATGAACCAAGAATTGTTGTGATGGAATATTGTGGAGATTCTTCAA
AACCAAAAACAGGTTTAGTTGGAAAAGGAATTTGTTTTGACTCTGGGGGATATAATTTAAAACCATCAAATTATATGGAA
GGTATGAAATTTGATATGTCTGGAGCAGCGGTTGTATTATCTGCAACTATGGCTTTAGCCAAAGCAAAAGCAAAAGCAAA
TATTGTTGCAGTAGGTATGTTCACTGATAATAAAATTGGACAGAATGCTACATTACCTGATTCTGTAATTGAATCAATGA
GTGGTAAAAAAGTATTGATATTTGATACTGATGCAGAAGGTCGACTTGTTTTAGCCGATGGAATTAGTTATGTTGTTAAA
GAAAAGCAAGTAGATCAAATAATTGATGCTTCAACTTTAACAGGAACAGTTTTATTTGCTTTAGGTCATAATGCAACTGG
AGCATTTTCTCATTCTGATAAAATGATAAATGAATTGAATGAAGCTTCACAAAAATCTGGTGAAAGATTTTGAAGATTAC
CAATTTTCAAAGAACATTTAAAACAAGTAAAAAAAGATGCTGCACCTTTAGCAGATTTAACTAATGCATGTAGAGTTAGA
TATGAAGATTGTTCATATGCAGCAGCCTTCTTGAATGAGTTTGCAGAAAATAAACCTTTCTTGCACTTAGATATAGCTGG
TACAGCTGATAAAGAAAATAAAGGTCAAGGTGTTTTGGTTAAAACGTTATTTGAAATGTTGAAATAA

Upstream 100 bases:

>100_bases
TTTGTCATCAACGTTCATATTATTAATACTAACCTCTATCAAAATATATACAAAACGACAAAATCCAAAAGAAATAATTA
AATAAAAAAGGAGATTGAAT

Downstream 100 bases:

>100_bases
AGTGGAATTCCACTTTTTTCTTTTATTAAGTACTTATTGTTTTAGGTATTAAGTGCTATAATTTTTTTAAATAGAAAGAT
GGTATATATTTATGATTACA

Product: cytosol aminopeptidase (leucine aminopeptidase)

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MIQINETKNTIKIVGVKDNSKNTFIKNKVGVPTLISEDKTIYIVFKKDEETFTEQIKKGIEATISNFDFDVDIDIDSFEN
VCDGISKEEIIKTVYETISFSTHKGLSIEKVEKETKYNLIFTGKHQELINQLTIVCEYINYARDLQDTPPNIGTSEYYAD
KLVSDAKDIKGLKVTILGRKEATKLNMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSNYME
GMKFDMSGAAVVLSATMALAKAKAKANIVAVGMFTDNKIGQNATLPDSVIESMSGKKVLIFDTDAEGRLVLADGISYVVK
EKQVDQIIDASTLTGTVLFALGHNATGAFSHSDKMINELNEASQKSGERFWRLPIFKEHLKQVKKDAAPLADLTNACRVR
YEDCSYAAAFLNEFAENKPFLHLDIAGTADKENKGQGVLVKTLFEMLK

Sequences:

>Translated_448_residues
MIQINETKNTIKIVGVKDNSKNTFIKNKVGVPTLISEDKTIYIVFKKDEETFTEQIKKGIEATISNFDFDVDIDIDSFEN
VCDGISKEEIIKTVYETISFSTHKGLSIEKVEKETKYNLIFTGKHQELINQLTIVCEYINYARDLQDTPPNIGTSEYYAD
KLVSDAKDIKGLKVTILGRKEATKLNMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSNYME
GMKFDMSGAAVVLSATMALAKAKAKANIVAVGMFTDNKIGQNATLPDSVIESMSGKKVLIFDTDAEGRLVLADGISYVVK
EKQVDQIIDASTLTGTVLFALGHNATGAFSHSDKMINELNEASQKSGERF*RLPIFKEHLKQVKKDAAPLADLTNACRVR
YEDCSYAAAFLNEFAENKPFLHLDIAGTADKENKGQGVLVKTLFEMLK
>Mature_448_residues
MIQINETKNTIKIVGVKDNSKNTFIKNKVGVPTLISEDKTIYIVFKKDEETFTEQIKKGIEATISNFDFDVDIDIDSFEN
VCDGISKEEIIKTVYETISFSTHKGLSIEKVEKETKYNLIFTGKHQELINQLTIVCEYINYARDLQDTPPNIGTSEYYAD
KLVSDAKDIKGLKVTILGRKEATKLNMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSNYME
GMKFDMSGAAVVLSATMALAKAKAKANIVAVGMFTDNKIGQNATLPDSVIESMSGKKVLIFDTDAEGRLVLADGISYVVK
EKQVDQIIDASTLTGTVLFALGHNATGAFSHSDKMINELNEASQKSGERF*RLPIFKEHLKQVKKDAAPLADLTNACRVR
YEDCSYAAAFLNEFAENKPFLHLDIAGTADKENKGQGVLVKTLFEMLK

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=338, Percent_Identity=34.9112426035503, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI47155554, Length=355, Percent_Identity=30.4225352112676, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1790710, Length=431, Percent_Identity=32.9466357308585, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082123, Length=367, Percent_Identity=34.6049046321526, Blast_Score=145, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17556903, Length=310, Percent_Identity=31.9354838709677, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17565172, Length=222, Percent_Identity=32.8828828828829, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI20129969, Length=296, Percent_Identity=32.7702702702703, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24661038, Length=286, Percent_Identity=31.8181818181818, Blast_Score=128, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21355725, Length=286, Percent_Identity=31.4685314685315, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI221379063, Length=326, Percent_Identity=31.2883435582822, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI221379062, Length=326, Percent_Identity=31.2883435582822, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI21357381, Length=326, Percent_Identity=31.2883435582822, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24662227, Length=294, Percent_Identity=30.6122448979592, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161077148, Length=294, Percent_Identity=29.9319727891156, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI20130057, Length=294, Percent_Identity=29.9319727891156, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355645, Length=294, Percent_Identity=28.9115646258503, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24662223, Length=294, Percent_Identity=28.9115646258503, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI19922386, Length=294, Percent_Identity=30.2721088435374, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129963, Length=290, Percent_Identity=29.3103448275862, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646701, Length=221, Percent_Identity=30.316742081448, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646703, Length=221, Percent_Identity=30.316742081448, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21358201, Length=221, Percent_Identity=30.316742081448, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 49188; Mature: 49188

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQINETKNTIKIVGVKDNSKNTFIKNKVGVPTLISEDKTIYIVFKKDEETFTEQIKKGI
CEEECCCCCEEEEEEEECCCCCCEEECCCCCCEEECCCCEEEEEEECCHHHHHHHHHHHH
EATISNFDFDVDIDIDSFENVCDGISKEEIIKTVYETISFSTHKGLSIEKVEKETKYNLI
HHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEE
FTGKHQELINQLTIVCEYINYARDLQDTPPNIGTSEYYADKLVSDAKDIKGLKVTILGRK
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
EATKLNMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSNYME
CCCEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHC
GMKFDMSGAAVVLSATMALAKAKAKANIVAVGMFTDNKIGQNATLPDSVIESMSGKKVLI
CCEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCCEEEE
FDTDAEGRLVLADGISYVVKEKQVDQIIDASTLTGTVLFALGHNATGAFSHSDKMINELN
EECCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHH
EASQKSGERFRLPIFKEHLKQVKKDAAPLADLTNACRVRYEDCSYAAAFLNEFAENKPFL
HHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHEEEECCHHHHHHHHHHHHCCCCEE
HLDIAGTADKENKGQGVLVKTLFEMLK
EEEECCCCCCCCCCCEEHHHHHHHHHC
>Mature Secondary Structure
MIQINETKNTIKIVGVKDNSKNTFIKNKVGVPTLISEDKTIYIVFKKDEETFTEQIKKGI
CEEECCCCCEEEEEEEECCCCCCEEECCCCCCEEECCCCEEEEEEECCHHHHHHHHHHHH
EATISNFDFDVDIDIDSFENVCDGISKEEIIKTVYETISFSTHKGLSIEKVEKETKYNLI
HHHHHCCCEEEEECHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCEEEE
FTGKHQELINQLTIVCEYINYARDLQDTPPNIGTSEYYADKLVSDAKDIKGLKVTILGRK
EECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
EATKLNMGLFLGVNAGSAHEPRIVVMEYCGDSSKPKTGLVGKGICFDSGGYNLKPSNYME
CCCEECCEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCCHHHC
GMKFDMSGAAVVLSATMALAKAKAKANIVAVGMFTDNKIGQNATLPDSVIESMSGKKVLI
CCEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHCCCCEEEE
FDTDAEGRLVLADGISYVVKEKQVDQIIDASTLTGTVLFALGHNATGAFSHSDKMINELN
EECCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHEEEEEEECCCCCCCCCHHHHHHHHHH
EASQKSGERFRLPIFKEHLKQVKKDAAPLADLTNACRVRYEDCSYAAAFLNEFAENKPFL
HHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHEEEECCHHHHHHHHHHHHCCCCEE
HLDIAGTADKENKGQGVLVKTLFEMLK
EEEECCCCCCCCCCCEEHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]