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The map label for this gene is hemK [H]

Identifier: 50085502

GI number: 50085502

Start: 2381237

End: 2382061

Strand: Reverse

Name: hemK [H]

Synonym: ACIAD2417

Alternate gene names: 50085502

Gene position: 2382061-2381237 (Counterclockwise)

Preceding gene: 161349962

Following gene: 50085501

Centisome position: 66.19

GC content: 42.42

Gene sequence:

>825_bases
ATGAATATCGGACAAGCACTCAACTTACGTGGTGAATCTGACAGTTATGAACGTCAGGAAGCAATGTGGTTGCTTGAACA
TATTTTAGAGTTGAACAGTCTTGAGCTAAAAATGCGTCAGATGCAGATACTGACGCAGGAGCAGGAACAACAGTATTTAG
ATGGTCTGATGCGACTTTCTAAAGGCGAACCTCTGGCTTATATTACCGGTTCACAGCCATTCTGGAGTCTAGATTTAAAT
GTGACTCCTGATACGCTGGTGCCACGACCAGATACCGAAATTTTGATTGAAACCGTATTGTCGCTAGATTTACCTGAACA
TTGTTCGATGGTCGATTTAGGAACGGGTACAGGGGCAATTGCTTTATCATTAGCAAAAGAACGACCTTACTGGAAAATTC
TGGCTACAGATATTTATTTCCCAACGCTGGAAGTAGCTCAGTCCAATGCGAAAAAGCATGCTTTGAATCAGGTTAAGTTT
TTATGTAGTGCTTGGTTTGAAGCTATTCCATCGCAAAAATTTGACCTCATTGTGTCTAATCCGCCATATATAGATGCTAA
TGATGAACATATGCTAAATTTGGGCACTGAACCACGCCGAGCTTTGGTTGCAGACAAGCACGGATTGGCAGATCTTGAGC
AGATTATTGGGCAGGCAGTGCTGTGGTTGAACACGCACGGCTGGATTGCAGTTGAACATGGTTATGATCAGGGGCAGGCT
GTTTGTCAGTTATTGATGGATCATAATTTTGCTAAAGTACAAACATGCAAGGACTATGGCGGTAATGATCGCATTAGTTT
AGGACAATGGTTAGCCGATACTTGA

Upstream 100 bases:

>100_bases
ATGCAATTATGGAAGGTGATTTGACTGAACTGCTGGATAGTTTACACCGTGAATATCAGGCTGATCAGTTGGCTATGTTG
GCACAAGAGAATGGTGGTTA

Downstream 100 bases:

>100_bases
TACTCGATAAACCCTGTATTGGCACTAAAAATTTATGTAACTAAAGATACCTATAAAATACGTTTGATTTTAAATAAAAA
TGTCTGTTTTATTGATCGTT

Product: methyl transferase

Products: NA

Alternate protein names: M.StyLTHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNIGQALNLRGESDSYERQEAMWLLEHILELNSLELKMRQMQILTQEQEQQYLDGLMRLSKGEPLAYITGSQPFWSLDLN
VTPDTLVPRPDTEILIETVLSLDLPEHCSMVDLGTGTGAIALSLAKERPYWKILATDIYFPTLEVAQSNAKKHALNQVKF
LCSAWFEAIPSQKFDLIVSNPPYIDANDEHMLNLGTEPRRALVADKHGLADLEQIIGQAVLWLNTHGWIAVEHGYDQGQA
VCQLLMDHNFAKVQTCKDYGGNDRISLGQWLADT

Sequences:

>Translated_274_residues
MNIGQALNLRGESDSYERQEAMWLLEHILELNSLELKMRQMQILTQEQEQQYLDGLMRLSKGEPLAYITGSQPFWSLDLN
VTPDTLVPRPDTEILIETVLSLDLPEHCSMVDLGTGTGAIALSLAKERPYWKILATDIYFPTLEVAQSNAKKHALNQVKF
LCSAWFEAIPSQKFDLIVSNPPYIDANDEHMLNLGTEPRRALVADKHGLADLEQIIGQAVLWLNTHGWIAVEHGYDQGQA
VCQLLMDHNFAKVQTCKDYGGNDRISLGQWLADT
>Mature_274_residues
MNIGQALNLRGESDSYERQEAMWLLEHILELNSLELKMRQMQILTQEQEQQYLDGLMRLSKGEPLAYITGSQPFWSLDLN
VTPDTLVPRPDTEILIETVLSLDLPEHCSMVDLGTGTGAIALSLAKERPYWKILATDIYFPTLEVAQSNAKKHALNQVKF
LCSAWFEAIPSQKFDLIVSNPPYIDANDEHMLNLGTEPRRALVADKHGLADLEQIIGQAVLWLNTHGWIAVEHGYDQGQA
VCQLLMDHNFAKVQTCKDYGGNDRISLGQWLADT

Specific function: Methylates the translation termination release factor RF1 on 'Gln-235' and RF2 on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=274, Percent_Identity=27.007299270073, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787463, Length=257, Percent_Identity=45.9143968871595, Blast_Score=208, Evalue=3e-55,
Organism=Escherichia coli, GI87082085, Length=201, Percent_Identity=30.8457711442786, Blast_Score=85, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24582226, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30890; Mature: 30890

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGQALNLRGESDSYERQEAMWLLEHILELNSLELKMRQMQILTQEQEQQYLDGLMRLS
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KGEPLAYITGSQPFWSLDLNVTPDTLVPRPDTEILIETVLSLDLPEHCSMVDLGTGTGAI
CCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCE
ALSLAKERPYWKILATDIYFPTLEVAQSNAKKHALNQVKFLCSAWFEAIPSQKFDLIVSN
EEEECCCCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
PPYIDANDEHMLNLGTEPRRALVADKHGLADLEQIIGQAVLWLNTHGWIAVEHGYDQGQA
CCEECCCCCEEEECCCCCHHHHHHCCCCHHHHHHHHHHHEEEEECCCEEEEECCCCHHHH
VCQLLMDHNFAKVQTCKDYGGNDRISLGQWLADT
HHHHHHHCCCHHHHHHHHCCCCCCEEHHHHHCCC
>Mature Secondary Structure
MNIGQALNLRGESDSYERQEAMWLLEHILELNSLELKMRQMQILTQEQEQQYLDGLMRLS
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KGEPLAYITGSQPFWSLDLNVTPDTLVPRPDTEILIETVLSLDLPEHCSMVDLGTGTGAI
CCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCE
ALSLAKERPYWKILATDIYFPTLEVAQSNAKKHALNQVKFLCSAWFEAIPSQKFDLIVSN
EEEECCCCCCEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
PPYIDANDEHMLNLGTEPRRALVADKHGLADLEQIIGQAVLWLNTHGWIAVEHGYDQGQA
CCEECCCCCEEEECCCCCHHHHHHCCCCHHHHHHHHHHHEEEEECCCEEEEECCCCHHHH
VCQLLMDHNFAKVQTCKDYGGNDRISLGQWLADT
HHHHHHHCCCHHHHHHHHCCCCCCEEHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 2544564 [H]