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The map label for this gene is 50085475

Identifier: 50085475

GI number: 50085475

Start: 2344399

End: 2345250

Strand: Reverse

Name: 50085475

Synonym: ACIAD2388

Alternate gene names: NA

Gene position: 2345250-2344399 (Counterclockwise)

Preceding gene: 50085476

Following gene: 50085474

Centisome position: 65.17

GC content: 41.67

Gene sequence:

>852_bases
ATGCATATTGCGACCATTGACTACTCTGCCTCAAATGCAGGACAACTTTTTGCACAGTCTTTGCATGATACTGGATTTGC
TGTCATCGTGAATCATCCATTAGATTGGCCCTTGATCCAGTTGATCTATACCGAATGGTTGGCATTTTTTAAGTCGGATG
CTAAAAATCAATATCGAGTTCGTCCCGAGCAAAAAGGTGGTTTTTTCCCGACGGATTTGTCTGAAACAGCAAAAGGAGAA
ACAGTTAAAGATTTGAAAGAGTTTTTTCATATCTTTCCATCTGGCGTTTTTCCTCATGAATTATCGACAAATACACATCA
TTATTATGAGCAGGCATTAATACTTGCCAAGCAGCTATTACAATGGATTCAGGACTTTTCTCCATCAAATGTGCAAAATG
CCTATCATGAGCCTTTACCCGCCATGATTGAAGGTTCTGAAAGCTTATTGCGTGTTTTAAGGTATACCCCAGTCGATGTA
GACCATGATTATATTCGTGCTGCGGCGCATGAAGATATTAATCTGATTACCATCTTGCCTGCGGCCACAGCACCAGGGTT
ACAAGTTAAAGATGCAACGGGCGAGTGGTATGATGTGCCTGTCGAGCCAAATTCTTTGATTATTAATATTGGCGATATGT
TGCAAGAAGCGTCGCAATTTTATTATCCGTCTACCACACATCGTGTTGTGAAATATAAAGATCAGGATCGCCTGCAAGAA
CGAATTTCTATGCCGTTCTTTTTACAGCCGCGTGGTGATGTGGTGTTGTCAGCTCGCTATACCGCAGGCAGCTATCATGC
AGAACGCATGCGTGAACTCAAAGTCATTCAGGATGGAAAAATCTTGCTCTAA

Upstream 100 bases:

>100_bases
ATCTTTGATGTTAAATAAATACATACACCAATAGCCGTATTTTACGACATAAATACGGCTTATTTTTTGCTTAGATCAGT
ATCATAAATATGGAGCTTCT

Downstream 100 bases:

>100_bases
ATGAGCATGTGATTAAGCGGTTATTGAATGATGAATTTCTGATTGAAATTCAGGATATTTTATATAAGGGTAAGTGAATG
ACACATTCTCCTCATCAAAT

Product: oxidoreductase

Products: isopenicillin N; H2O

Alternate protein names: Oxidoreductase; 2OG-Fe(II) Oxygenase Family Oxidoreductase; Isopenicillin N Synthase; Oxidoreductase 2OG-Fe(II) Oxygenase Family; 2OG-Fe(II) Oxygenase Superfamily Protein; Iron/Ascorbate Family Oxidoreductase; Oxidoreductase Iron/Ascorbate Family Protein; Iron/Ascorbate Oxidoreductase; Flavonol Synthase/Dioxygenase; 2og-Fe(Ii) Oxygenase; Oxidoreductase 2OG-Fe(II) Oxygenase Family Protein; Oxidoreductase Iron/Ascorbate Family; Fe-Containing Oxidoreductase; Isopenicillin-N Synthase; Isopenicillin N Synthase And Related Dioxygenases; Oxidoreductase 2og-Fe(Ii) Oxygenase Family; Oxygenase/Oxidoreductase; Iron/Ascorbate-Dependent Oxidoreductase Family Protein

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MHIATIDYSASNAGQLFAQSLHDTGFAVIVNHPLDWPLIQLIYTEWLAFFKSDAKNQYRVRPEQKGGFFPTDLSETAKGE
TVKDLKEFFHIFPSGVFPHELSTNTHHYYEQALILAKQLLQWIQDFSPSNVQNAYHEPLPAMIEGSESLLRVLRYTPVDV
DHDYIRAAAHEDINLITILPAATAPGLQVKDATGEWYDVPVEPNSLIINIGDMLQEASQFYYPSTTHRVVKYKDQDRLQE
RISMPFFLQPRGDVVLSARYTAGSYHAERMRELKVIQDGKILL

Sequences:

>Translated_283_residues
MHIATIDYSASNAGQLFAQSLHDTGFAVIVNHPLDWPLIQLIYTEWLAFFKSDAKNQYRVRPEQKGGFFPTDLSETAKGE
TVKDLKEFFHIFPSGVFPHELSTNTHHYYEQALILAKQLLQWIQDFSPSNVQNAYHEPLPAMIEGSESLLRVLRYTPVDV
DHDYIRAAAHEDINLITILPAATAPGLQVKDATGEWYDVPVEPNSLIINIGDMLQEASQFYYPSTTHRVVKYKDQDRLQE
RISMPFFLQPRGDVVLSARYTAGSYHAERMRELKVIQDGKILL
>Mature_283_residues
MHIATIDYSASNAGQLFAQSLHDTGFAVIVNHPLDWPLIQLIYTEWLAFFKSDAKNQYRVRPEQKGGFFPTDLSETAKGE
TVKDLKEFFHIFPSGVFPHELSTNTHHYYEQALILAKQLLQWIQDFSPSNVQNAYHEPLPAMIEGSESLLRVLRYTPVDV
DHDYIRAAAHEDINLITILPAATAPGLQVKDATGEWYDVPVEPNSLIINIGDMLQEASQFYYPSTTHRVVKYKDQDRLQE
RISMPFFLQPRGDVVLSARYTAGSYHAERMRELKVIQDGKILL

Specific function: Unknown

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.21.3.1

Molecular weight: Translated: 32322; Mature: 32322

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIATIDYSASNAGQLFAQSLHDTGFAVIVNHPLDWPLIQLIYTEWLAFFKSDAKNQYRV
CEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEE
RPEQKGGFFPTDLSETAKGETVKDLKEFFHIFPSGVFPHELSTNTHHYYEQALILAKQLL
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QWIQDFSPSNVQNAYHEPLPAMIEGSESLLRVLRYTPVDVDHDYIRAAAHEDINLITILP
HHHHCCCCCCHHHHHHCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEE
AATAPGLQVKDATGEWYDVPVEPNSLIINIGDMLQEASQFYYPSTTHRVVKYKDQDRLQE
CCCCCCCEEECCCCCEEECCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
RISMPFFLQPRGDVVLSARYTAGSYHAERMRELKVIQDGKILL
HHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEC
>Mature Secondary Structure
MHIATIDYSASNAGQLFAQSLHDTGFAVIVNHPLDWPLIQLIYTEWLAFFKSDAKNQYRV
CEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEE
RPEQKGGFFPTDLSETAKGETVKDLKEFFHIFPSGVFPHELSTNTHHYYEQALILAKQLL
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QWIQDFSPSNVQNAYHEPLPAMIEGSESLLRVLRYTPVDVDHDYIRAAAHEDINLITILP
HHHHCCCCCCHHHHHHCCCCHHHCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEEE
AATAPGLQVKDATGEWYDVPVEPNSLIINIGDMLQEASQFYYPSTTHRVVKYKDQDRLQE
CCCCCCCEEECCCCCEEECCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCHHHHHH
RISMPFFLQPRGDVVLSARYTAGSYHAERMRELKVIQDGKILL
HHCCCEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine; O2

Specific reaction: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA