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The map label for this gene is iunH [H]

Identifier: 50085474

GI number: 50085474

Start: 2343374

End: 2344321

Strand: Reverse

Name: iunH [H]

Synonym: ACIAD2386

Alternate gene names: 50085474

Gene position: 2344321-2343374 (Counterclockwise)

Preceding gene: 50085475

Following gene: 50085472

Centisome position: 65.14

GC content: 44.2

Gene sequence:

>948_bases
ATGACACATTCTCCTCATCAAATTATTATTGATACAGATCCAGGTCAGGATGATGCAGTTGCAATTTTATTGGCACTGGC
GAGCCCTGAAGTAAAGGTACTTGCGATTACAACGGTCGCTGGAAATGTGCCATTGGCATTGACCAGTGCAAATGCACGTA
AAGTATGTGAATTGGCTGGACGTCCAGATATTTCTGTTTTCGCTGGATGCGATCGTCCGTTGAAACGTCCATTAATTACC
GCCGAAAATGTACATGGAAAAACAGGACTCGATGGTATTGAGTTACCTGAGCCGCAAATGCCTTTGCAGGCCCAGCACTC
AGTTGATTTTATTATTGAAACTCTGCGAAATGCACCTGAGAAAACAATTACGATCTGTTCTTTGGGGCCGATGACCAATA
CTGCTCAGGCACTATTAAAAGCTCCAGATATTGCAGCGCGAGTTAAGCGAATTGTCTTGATGGGGGGCGGCTTTTTTGAA
GGAGGAAATATTACACCTTCGGCAGAATTTAATATGTTTGTTGACCCAGACGCAGCGAAAATTGTATTTGCTGCGGGTAT
TCCATTAACTGTGATTCCACTTGATGTCACACATCAAGTCTTAACCTCGAAAGAATGGGTCGATGGTTTGCGCCGTATGG
ATAATCAGGTCGGTCCTGCTGTAGCAGCCTGGACAGATTTCTTTGAACGTTATGATATGGAAAAATACGGTTCTGAAGGC
GCACCATTACATGATCCTTGTGTCATCGGCTATATGATCAAACCTGAACTATTCTCTGGTCGTTTTATCAATGTCGAGAT
TGAAGCGGATAGCCCATTGACAGTGGGCGCTATGGTTGCAGACTGGTGGAATGTCACTGGTCGCACTGCAAATGCAGAAT
ATTTACGTGATATTGATCGTGATGGCTTCTTTGATTTGCTATCAGAGCGTCTGGCAACGTATCAGTAG

Upstream 100 bases:

>100_bases
AGGATGGAAAAATCTTGCTCTAAATGAGCATGTGATTAAGCGGTTATTGAATGATGAATTTCTGATTGAAATTCAGGATA
TTTTATATAAGGGTAAGTGA

Downstream 100 bases:

>100_bases
ATTGTATCTTAAATAGCATGAATCATCATGATATTGATCATGGTGATTCATTCAAAAGTTTAACAAGATTAAGCGCATTG
ATTTGACGAAGTTTTTTTCT

Product: inosine-uridine preferring nucleoside hydrolase

Products: D-ribose; purine

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTHSPHQIIIDTDPGQDDAVAILLALASPEVKVLAITTVAGNVPLALTSANARKVCELAGRPDISVFAGCDRPLKRPLIT
AENVHGKTGLDGIELPEPQMPLQAQHSVDFIIETLRNAPEKTITICSLGPMTNTAQALLKAPDIAARVKRIVLMGGGFFE
GGNITPSAEFNMFVDPDAAKIVFAAGIPLTVIPLDVTHQVLTSKEWVDGLRRMDNQVGPAVAAWTDFFERYDMEKYGSEG
APLHDPCVIGYMIKPELFSGRFINVEIEADSPLTVGAMVADWWNVTGRTANAEYLRDIDRDGFFDLLSERLATYQ

Sequences:

>Translated_315_residues
MTHSPHQIIIDTDPGQDDAVAILLALASPEVKVLAITTVAGNVPLALTSANARKVCELAGRPDISVFAGCDRPLKRPLIT
AENVHGKTGLDGIELPEPQMPLQAQHSVDFIIETLRNAPEKTITICSLGPMTNTAQALLKAPDIAARVKRIVLMGGGFFE
GGNITPSAEFNMFVDPDAAKIVFAAGIPLTVIPLDVTHQVLTSKEWVDGLRRMDNQVGPAVAAWTDFFERYDMEKYGSEG
APLHDPCVIGYMIKPELFSGRFINVEIEADSPLTVGAMVADWWNVTGRTANAEYLRDIDRDGFFDLLSERLATYQ
>Mature_314_residues
THSPHQIIIDTDPGQDDAVAILLALASPEVKVLAITTVAGNVPLALTSANARKVCELAGRPDISVFAGCDRPLKRPLITA
ENVHGKTGLDGIELPEPQMPLQAQHSVDFIIETLRNAPEKTITICSLGPMTNTAQALLKAPDIAARVKRIVLMGGGFFEG
GNITPSAEFNMFVDPDAAKIVFAAGIPLTVIPLDVTHQVLTSKEWVDGLRRMDNQVGPAVAAWTDFFERYDMEKYGSEGA
PLHDPCVIGYMIKPELFSGRFINVEIEADSPLTVGAMVADWWNVTGRTANAEYLRDIDRDGFFDLLSERLATYQ

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=308, Percent_Identity=46.7532467532467, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1788486, Length=312, Percent_Identity=39.1025641025641, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786213, Length=305, Percent_Identity=34.4262295081967, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI32565474, Length=199, Percent_Identity=37.1859296482412, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17565698, Length=299, Percent_Identity=27.4247491638796, Blast_Score=94, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320608, Length=261, Percent_Identity=34.0996168582375, Blast_Score=129, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24583915, Length=201, Percent_Identity=31.8407960199005, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24641839, Length=210, Percent_Identity=31.4285714285714, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24641837, Length=203, Percent_Identity=33.0049261083744, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 34225; Mature: 34093

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSPHQIIIDTDPGQDDAVAILLALASPEVKVLAITTVAGNVPLALTSANARKVCELAG
CCCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCEEEECCCHHHHHHHCC
RPDISVFAGCDRPLKRPLITAENVHGKTGLDGIELPEPQMPLQAQHSVDFIIETLRNAPE
CCCEEEEECCCCHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
KTITICSLGPMTNTAQALLKAPDIAARVKRIVLMGGGFFEGGNITPSAEFNMFVDPDAAK
CEEEEEECCCCCHHHHHHHCCCCHHHHHEEEEEECCCEECCCCCCCCCCEEEEECCCCCE
IVFAAGIPLTVIPLDVTHQVLTSKEWVDGLRRMDNQVGPAVAAWTDFFERYDMEKYGSEG
EEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHCCCC
APLHDPCVIGYMIKPELFSGRFINVEIEADSPLTVGAMVADWWNVTGRTANAEYLRDIDR
CCCCCCEEEEEEECCCCCCCEEEEEEEECCCCEEEHHHHHHHHCCCCCCCCHHHHHHCCC
DGFFDLLSERLATYQ
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
THSPHQIIIDTDPGQDDAVAILLALASPEVKVLAITTVAGNVPLALTSANARKVCELAG
CCCCCEEEEECCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCEEEECCCHHHHHHHCC
RPDISVFAGCDRPLKRPLITAENVHGKTGLDGIELPEPQMPLQAQHSVDFIIETLRNAPE
CCCEEEEECCCCHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
KTITICSLGPMTNTAQALLKAPDIAARVKRIVLMGGGFFEGGNITPSAEFNMFVDPDAAK
CEEEEEECCCCCHHHHHHHCCCCHHHHHEEEEEECCCEECCCCCCCCCCEEEEECCCCCE
IVFAAGIPLTVIPLDVTHQVLTSKEWVDGLRRMDNQVGPAVAAWTDFFERYDMEKYGSEG
EEEECCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHCCCC
APLHDPCVIGYMIKPELFSGRFINVEIEADSPLTVGAMVADWWNVTGRTANAEYLRDIDR
CCCCCCEEEEEEECCCCCCCEEEEEEEECCCCEEEHHHHHHHHCCCCCCCCHHHHHHCCC
DGFFDLLSERLATYQ
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA