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The map label for this gene is metR [H]

Identifier: 50085472

GI number: 50085472

Start: 2340640

End: 2341560

Strand: Reverse

Name: metR [H]

Synonym: ACIAD2384

Alternate gene names: 50085472

Gene position: 2341560-2340640 (Counterclockwise)

Preceding gene: 50085474

Following gene: 50085470

Centisome position: 65.07

GC content: 41.04

Gene sequence:

>921_bases
ATGCTCGAAATTCGTCATCTAAAAACTTTACTGGCTTTACGTGAGCATGGTTCGCTGGTTGCTGCTGCAAATGATTTGTG
TCTGACGCCTTCGGCTATATCTCATCAATTAAAAGAGCTTGATCACTGGTACGGTGTTGAGGTAGTTAACCGCCGTAGTC
GTCCTGTGACATTTTCAAATGTTGGCCAGCGTTTGCTTAAATTGGCAGATGATATTTTGCCACAGATTCAAATTGCACAA
AGCGATATTACTCGGATTGTACATGGGCAAACAGGACGTATTGTTTTCTCCTCAGAATGTCATAGCTGTTTTGATTGGTT
AATGCCCTTACTTAATCAATATCGTCAACAGTATCCTGATGTAGATCTTGACTTTGCTTCTGGCTTCGAAGCCAATCCAC
ACGACTTGTTGCAAACAGGAGAGTTTGATCTTTTGATCACAGCAGACCCTATTGCACTAAAAGGTATTGAGTATTTTCCA
ATTTTTGAATATGAGTCGCGTTTGGTATTGTCTAATACTCATCCTTTGGTTCGTGCAGAAAAAGTAACGGTTGAGGAGTT
GGCCGAGCAAACTCTGATTACTTATCCTGTAGACAAGCATCGTTTGGATATCATGGCACATCTGTTTATTCCAGCCAATA
TTCAACCTAAAGAGATCCGTACTACGGATTTAACCCAGATGTTGATTCAACTTGTAGCCAGTGGACGGGGTATTTCGGCT
TTACCAGATTGGGTGGTTAATGAATATGAGCAAAAAGGCTGGGTGACGAGCCGTCGTCTTGACTGCGTTTCTGCAAATGG
TTTACGCCGTACTTTATATGCTGGCTATCGGATTGAAGAAAAAGAGAAAAGCTACTTTGAAGGTTTCTTAAAGCAATTGG
CTAAATTTTCGCAAAAACGTGCTCATTATTATGCTAACTAG

Upstream 100 bases:

>100_bases
GATGAATCATTGTTCAGTTGTTTAAAATGAAAGCATTGAAAATCGCAAATTAAAGAGTTTCAGATTTTCAGCACTGCAAG
TATTTGAGAGATTGTTCTAC

Downstream 100 bases:

>100_bases
CAATATGTTATGTTGAATGATGACTTAACAAGATCTCACCATGAGTTAAAAAGAGAGGATATTTAATCAAAATTAAATAT
CCTGCTCGAAAATATATAAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MLEIRHLKTLLALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVTFSNVGQRLLKLADDILPQIQIAQ
SDITRIVHGQTGRIVFSSECHSCFDWLMPLLNQYRQQYPDVDLDFASGFEANPHDLLQTGEFDLLITADPIALKGIEYFP
IFEYESRLVLSNTHPLVRAEKVTVEELAEQTLITYPVDKHRLDIMAHLFIPANIQPKEIRTTDLTQMLIQLVASGRGISA
LPDWVVNEYEQKGWVTSRRLDCVSANGLRRTLYAGYRIEEKEKSYFEGFLKQLAKFSQKRAHYYAN

Sequences:

>Translated_306_residues
MLEIRHLKTLLALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVTFSNVGQRLLKLADDILPQIQIAQ
SDITRIVHGQTGRIVFSSECHSCFDWLMPLLNQYRQQYPDVDLDFASGFEANPHDLLQTGEFDLLITADPIALKGIEYFP
IFEYESRLVLSNTHPLVRAEKVTVEELAEQTLITYPVDKHRLDIMAHLFIPANIQPKEIRTTDLTQMLIQLVASGRGISA
LPDWVVNEYEQKGWVTSRRLDCVSANGLRRTLYAGYRIEEKEKSYFEGFLKQLAKFSQKRAHYYAN
>Mature_306_residues
MLEIRHLKTLLALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVTFSNVGQRLLKLADDILPQIQIAQ
SDITRIVHGQTGRIVFSSECHSCFDWLMPLLNQYRQQYPDVDLDFASGFEANPHDLLQTGEFDLLITADPIALKGIEYFP
IFEYESRLVLSNTHPLVRAEKVTVEELAEQTLITYPVDKHRLDIMAHLFIPANIQPKEIRTTDLTQMLIQLVASGRGISA
LPDWVVNEYEQKGWVTSRRLDCVSANGLRRTLYAGYRIEEKEKSYFEGFLKQLAKFSQKRAHYYAN

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=291, Percent_Identity=41.9243986254296, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI157672245, Length=248, Percent_Identity=22.5806451612903, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1786401, Length=257, Percent_Identity=23.7354085603113, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788887, Length=252, Percent_Identity=25.7936507936508, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790208, Length=247, Percent_Identity=25.1012145748988, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790399, Length=313, Percent_Identity=27.1565495207668, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788296, Length=190, Percent_Identity=27.3684210526316, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787530, Length=297, Percent_Identity=20.2020202020202, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=256, Percent_Identity=23.046875, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081904, Length=200, Percent_Identity=28.5, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787879, Length=268, Percent_Identity=24.2537313432836, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35081; Mature: 35081

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIRHLKTLLALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVTFSN
CCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCCCCHHH
VGQRLLKLADDILPQIQIAQSDITRIVHGQTGRIVFSSECHSCFDWLMPLLNQYRQQYPD
HHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
VDLDFASGFEANPHDLLQTGEFDLLITADPIALKGIEYFPIFEYESRLVLSNTHPLVRAE
CCCCCCCCCCCCHHHHHHCCCEEEEEECCCCEECCCEEEEEEECCCEEEEECCCCCCEEH
KVTVEELAEQTLITYPVDKHRLDIMAHLFIPANIQPKEIRTTDLTQMLIQLVASGRGISA
HCCHHHHHHHHEEECCCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
LPDWVVNEYEQKGWVTSRRLDCVSANGLRRTLYAGYRIEEKEKSYFEGFLKQLAKFSQKR
CHHHHHHHHHHCCCCHHHHCCEECCCCHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHH
AHYYAN
HHCCCC
>Mature Secondary Structure
MLEIRHLKTLLALREHGSLVAAANDLCLTPSAISHQLKELDHWYGVEVVNRRSRPVTFSN
CCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHCCCCCCCCHHH
VGQRLLKLADDILPQIQIAQSDITRIVHGQTGRIVFSSECHSCFDWLMPLLNQYRQQYPD
HHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
VDLDFASGFEANPHDLLQTGEFDLLITADPIALKGIEYFPIFEYESRLVLSNTHPLVRAE
CCCCCCCCCCCCHHHHHHCCCEEEEEECCCCEECCCEEEEEEECCCEEEEECCCCCCEEH
KVTVEELAEQTLITYPVDKHRLDIMAHLFIPANIQPKEIRTTDLTQMLIQLVASGRGISA
HCCHHHHHHHHEEECCCCHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
LPDWVVNEYEQKGWVTSRRLDCVSANGLRRTLYAGYRIEEKEKSYFEGFLKQLAKFSQKR
CHHHHHHHHHHCCCCHHHHCCEECCCCHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHH
AHYYAN
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]