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The map label for this gene is rsuA [H]

Identifier: 50085470

GI number: 50085470

Start: 2338567

End: 2339286

Strand: Reverse

Name: rsuA [H]

Synonym: ACIAD2380

Alternate gene names: 50085470

Gene position: 2339286-2338567 (Counterclockwise)

Preceding gene: 50085472

Following gene: 50085466

Centisome position: 65.01

GC content: 37.08

Gene sequence:

>720_bases
CTGGATATCGATATGCTCTTGGAAAAAATGTTGCAGTCACAAGGTTTTGGTTCAAGAAAATACTGTCAGCAACTTATTAA
GAACGGAGCAGTTTCGATACAAGATGAAATTCAAAAAGATCTAAAATATCGAATTCATGATTTAACTGATTTTAATTTTC
AGGTTTTTGATCAAAATTACGTATATCGGGAAAAGGTATATATTATTTTAAATAAACCTAAAGGCTTTGAATGTTCTCAT
CAGCCACATCATCATCAAAGCGTATTCAGTTTACTGCCAGATTTATTAATTCAGCGCAATATTGCGGCTGTAGGGCGCTT
GGATCAGGATACTACAGGGCTGTTGTTACTGACTGATGATGGACAATACCTACACACGCTAACGCATCCCAAGAAGCATG
TTCCAAAGGTATATCATGTGGCTACGATTGATCCAATTACAGTTGATCAAATGCATCAATTAGAACAAGGTGTTGAATTA
CATAATGAAAAAGGTGTTTTTGCAGCGACAAATGTTACTTTGCTCAATGAAACACAATTGAGCATGACGATTCATCAAGG
TGTGTACCACCAAGTCAAACGAATGATTGCTGCTGTAGGCAACAAAGTGACTGCCTTGCATCGCCATAAGATTGGTCAAT
TAGAATTGCCAAATTTGGCAGAAGGTGAGTGGATGTATCTGACCGAAACACAAAAATTAGCGGCTCAACAAATCACTTAA

Upstream 100 bases:

>100_bases
GAAGCTTATAAATCGCATCCACTTAACTGCCGCACAGGCAGCTTAACTATTCAATCAATTGACTTAAAAAAATAAGTTAC
ACTAGGATGTCTTTTTTATT

Downstream 100 bases:

>100_bases
GAAAACAATGATAGGTTTGGAATTCTTCGTCAAGGACGAACCCCATTTTTTGATAGAGCCGCATAGATTCAATATTATTT
TTTTGTGTTTCTAGTGAAAT

Product: 16S rRNA pseudouridylate 516 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MDIDMLLEKMLQSQGFGSRKYCQQLIKNGAVSIQDEIQKDLKYRIHDLTDFNFQVFDQNYVYREKVYIILNKPKGFECSH
QPHHHQSVFSLLPDLLIQRNIAAVGRLDQDTTGLLLLTDDGQYLHTLTHPKKHVPKVYHVATIDPITVDQMHQLEQGVEL
HNEKGVFAATNVTLLNETQLSMTIHQGVYHQVKRMIAAVGNKVTALHRHKIGQLELPNLAEGEWMYLTETQKLAAQQIT

Sequences:

>Translated_239_residues
MDIDMLLEKMLQSQGFGSRKYCQQLIKNGAVSIQDEIQKDLKYRIHDLTDFNFQVFDQNYVYREKVYIILNKPKGFECSH
QPHHHQSVFSLLPDLLIQRNIAAVGRLDQDTTGLLLLTDDGQYLHTLTHPKKHVPKVYHVATIDPITVDQMHQLEQGVEL
HNEKGVFAATNVTLLNETQLSMTIHQGVYHQVKRMIAAVGNKVTALHRHKIGQLELPNLAEGEWMYLTETQKLAAQQIT
>Mature_239_residues
MDIDMLLEKMLQSQGFGSRKYCQQLIKNGAVSIQDEIQKDLKYRIHDLTDFNFQVFDQNYVYREKVYIILNKPKGFECSH
QPHHHQSVFSLLPDLLIQRNIAAVGRLDQDTTGLLLLTDDGQYLHTLTHPKKHVPKVYHVATIDPITVDQMHQLEQGVEL
HNEKGVFAATNVTLLNETQLSMTIHQGVYHQVKRMIAAVGNKVTALHRHKIGQLELPNLAEGEWMYLTETQKLAAQQIT

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=38.961038961039, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787524, Length=231, Percent_Identity=31.6017316017316, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI87081838, Length=215, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790453, Length=237, Percent_Identity=23.6286919831224, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 27444; Mature: 27444

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIDMLLEKMLQSQGFGSRKYCQQLIKNGAVSIQDEIQKDLKYRIHDLTDFNFQVFDQNY
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCE
VYREKVYIILNKPKGFECSHQPHHHQSVFSLLPDLLIQRNIAAVGRLDQDTTGLLLLTDD
EEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
GQYLHTLTHPKKHVPKVYHVATIDPITVDQMHQLEQGVELHNEKGVFAATNVTLLNETQL
CCEEEECCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHH
SMTIHQGVYHQVKRMIAAVGNKVTALHRHKIGQLELPNLAEGEWMYLTETQKLAAQQIT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHCCC
>Mature Secondary Structure
MDIDMLLEKMLQSQGFGSRKYCQQLIKNGAVSIQDEIQKDLKYRIHDLTDFNFQVFDQNY
CCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEECCCE
VYREKVYIILNKPKGFECSHQPHHHQSVFSLLPDLLIQRNIAAVGRLDQDTTGLLLLTDD
EEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
GQYLHTLTHPKKHVPKVYHVATIDPITVDQMHQLEQGVELHNEKGVFAATNVTLLNETQL
CCEEEECCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCHH
SMTIHQGVYHQVKRMIAAVGNKVTALHRHKIGQLELPNLAEGEWMYLTETQKLAAQQIT
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]