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The map label for this gene is paaF [C]

Identifier: 50085442

GI number: 50085442

Start: 2312548

End: 2313669

Strand: Reverse

Name: paaF [C]

Synonym: ACIAD2351

Alternate gene names: 50085442

Gene position: 2313669-2312548 (Counterclockwise)

Preceding gene: 50085443

Following gene: 50085441

Centisome position: 64.29

GC content: 41.89

Gene sequence:

>1122_bases
ATGACTAACTCTCCCAATGTAACAAGTTATCATCCTGATCTAATTGTAGAAGAGGCAAAAAACGGCTGGCGTATCATACG
TCTTAACCGCCCGAAGTCTTTGCATGCACTTGATGAATCAATTGTCGTTGCACTGCTACAAGTATTTGAGGAATTTCATA
CAGATGATGCTGTAAAAGCGATCTGGTTTGATTCAACCACACCAAAAGCATTTTGTGCTGGTGGAGATGTTCGTAAATTA
AGACAATTGGTAATTAATCAGGAAGTAGATACTGCCAAGCGTTTTTTTGAAAAAGAATATGCACTTGATCTTTTATTACA
TAATTACGCTAAACCTATTCTGGTTTGGGGTGAGGGTTATGTAATGGGCGGCGGATTGGGCTTATTTATGGCGGCTCCTT
TTAGGCTGGTTACACCCTATTCGCGACTTGCTATGCCTGAGATCAATATTGGCTTATATCCAGATGTAGGCGCTAGCCGA
TTCCTTGCAGACCGTGGGCCAATTGGGTTGTTTACAGGGCTGACAGGTTCAATTATGACAGCGGCAGGTGCTTATATCAT
TGGTTGGGCAACGCATATCTGTGATGCTCAGCGCGATAATGTACTTAATAAAGTGGTTAACATTGACTGGAGTCATTATC
CTGCTGGAGATTTTAGGGCAATTGACGATACGCTAAATAGTTTGCATCGTCCCGTGGGGCCTGGACCTTTACAGAACTCA
TTAGACGTCATTCATAGTGTATGTCGTGGGGTCGATTTCGAGCAGGATTATCAATCCATTATTGGTCTGATTGATGCGAG
AAGTGACTGGTTAAGACAAGCAAGTGAAAATCTACAAAAAGGGTCTCCAAGTACTGCCGCAATTACCTGGTTATTATGGC
AATGGGGTAAGAAGGTACACGCCTGGGAGGAAGTATTTAAACTTGAGGCACAAATTTCTGACTGGAAGATTCGCCATCCT
GATTTTGTAGAAGGTGTTCGTGCACGTTTGGTAGATAAAGATTTATCTCCCGCGTGGAAGGATACTCAGGATTTAAGTTT
GAAAGGTATTCTAGGTGATAATCCTCCTGTTACTACAATTCAAAGCTGGAACGATTTGTTAAAACACTATGGTATCATTT
AG

Upstream 100 bases:

>100_bases
CCGCAAATCGTGGCGGTTTTTTTGGTTGTAAAGTTTTTTCCAAAACAAATAATTATCTGAAACAACATGGCATTGAGCCT
ATAGATTGGCAGCTGGACGC

Downstream 100 bases:

>100_bases
TTCAAATGAGTCTCTCCGACACTTTTAATGATGAATACTGGATGCAGCTTGCTTACGAGCAAGCTGTACGCGCAGCCGAA
CACAACGAAGTTCCTGTAGG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MTNSPNVTSYHPDLIVEEAKNGWRIIRLNRPKSLHALDESIVVALLQVFEEFHTDDAVKAIWFDSTTPKAFCAGGDVRKL
RQLVINQEVDTAKRFFEKEYALDLLLHNYAKPILVWGEGYVMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASR
FLADRGPIGLFTGLTGSIMTAAGAYIIGWATHICDAQRDNVLNKVVNIDWSHYPAGDFRAIDDTLNSLHRPVGPGPLQNS
LDVIHSVCRGVDFEQDYQSIIGLIDARSDWLRQASENLQKGSPSTAAITWLLWQWGKKVHAWEEVFKLEAQISDWKIRHP
DFVEGVRARLVDKDLSPAWKDTQDLSLKGILGDNPPVTTIQSWNDLLKHYGII

Sequences:

>Translated_373_residues
MTNSPNVTSYHPDLIVEEAKNGWRIIRLNRPKSLHALDESIVVALLQVFEEFHTDDAVKAIWFDSTTPKAFCAGGDVRKL
RQLVINQEVDTAKRFFEKEYALDLLLHNYAKPILVWGEGYVMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASR
FLADRGPIGLFTGLTGSIMTAAGAYIIGWATHICDAQRDNVLNKVVNIDWSHYPAGDFRAIDDTLNSLHRPVGPGPLQNS
LDVIHSVCRGVDFEQDYQSIIGLIDARSDWLRQASENLQKGSPSTAAITWLLWQWGKKVHAWEEVFKLEAQISDWKIRHP
DFVEGVRARLVDKDLSPAWKDTQDLSLKGILGDNPPVTTIQSWNDLLKHYGII
>Mature_372_residues
TNSPNVTSYHPDLIVEEAKNGWRIIRLNRPKSLHALDESIVVALLQVFEEFHTDDAVKAIWFDSTTPKAFCAGGDVRKLR
QLVINQEVDTAKRFFEKEYALDLLLHNYAKPILVWGEGYVMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRF
LADRGPIGLFTGLTGSIMTAAGAYIIGWATHICDAQRDNVLNKVVNIDWSHYPAGDFRAIDDTLNSLHRPVGPGPLQNSL
DVIHSVCRGVDFEQDYQSIIGLIDARSDWLRQASENLQKGSPSTAAITWLLWQWGKKVHAWEEVFKLEAQISDWKIRHPD
FVEGVRARLVDKDLSPAWKDTQDLSLKGILGDNPPVTTIQSWNDLLKHYGII

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=346, Percent_Identity=32.0809248554913, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI37594469, Length=205, Percent_Identity=35.1219512195122, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI194097323, Length=200, Percent_Identity=29.5, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=359, Percent_Identity=30.6406685236769, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25144157, Length=359, Percent_Identity=30.6406685236769, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17554946, Length=207, Percent_Identity=28.9855072463768, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=380, Percent_Identity=26.8421052631579, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI28571729, Length=348, Percent_Identity=31.8965517241379, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI28571730, Length=348, Percent_Identity=31.8965517241379, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 41829; Mature: 41698

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSPNVTSYHPDLIVEEAKNGWRIIRLNRPKSLHALDESIVVALLQVFEEFHTDDAVKA
CCCCCCCCCCCCCHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IWFDSTTPKAFCAGGDVRKLRQLVINQEVDTAKRFFEKEYALDLLLHNYAKPILVWGEGY
EEECCCCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCE
VMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRFLADRGPIGLFTGLTGSIMT
EEECCHHHHHHCCHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCCHHHHH
AAGAYIIGWATHICDAQRDNVLNKVVNIDWSHYPAGDFRAIDDTLNSLHRPVGPGPLQNS
HCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHH
LDVIHSVCRGVDFEQDYQSIIGLIDARSDWLRQASENLQKGSPSTAAITWLLWQWGKKVH
HHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHH
AWEEVFKLEAQISDWKIRHPDFVEGVRARLVDKDLSPAWKDTQDLSLKGILGDNPPVTTI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHH
QSWNDLLKHYGII
HHHHHHHHHCCCC
>Mature Secondary Structure 
TNSPNVTSYHPDLIVEEAKNGWRIIRLNRPKSLHALDESIVVALLQVFEEFHTDDAVKA
CCCCCCCCCCCCHHEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IWFDSTTPKAFCAGGDVRKLRQLVINQEVDTAKRFFEKEYALDLLLHNYAKPILVWGEGY
EEECCCCCCCEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCE
VMGGGLGLFMAAPFRLVTPYSRLAMPEINIGLYPDVGASRFLADRGPIGLFTGLTGSIMT
EEECCHHHHHHCCHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEEECCCCHHHHH
AAGAYIIGWATHICDAQRDNVLNKVVNIDWSHYPAGDFRAIDDTLNSLHRPVGPGPLQNS
HCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHH
LDVIHSVCRGVDFEQDYQSIIGLIDARSDWLRQASENLQKGSPSTAAITWLLWQWGKKVH
HHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHH
AWEEVFKLEAQISDWKIRHPDFVEGVRARLVDKDLSPAWKDTQDLSLKGILGDNPPVTTI
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCHH
QSWNDLLKHYGII
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA