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The map label for this gene is thlA [H]

Identifier: 50084773

GI number: 50084773

Start: 1610288

End: 1611469

Strand: Direct

Name: thlA [H]

Synonym: ACIAD1612

Alternate gene names: 50084773

Gene position: 1610288-1611469 (Clockwise)

Preceding gene: 50084772

Following gene: 50084774

Centisome position: 44.75

GC content: 43.49

Gene sequence:

>1182_bases
ATGAACAATGAAGTTGTGATTTTGAGTGCAATACGCACACCTATGGGTGGTTTTCTGGGTGAGTTAAAACCATTTTCGGC
CACCGAACTTGGAAGTTTTGCTATTCAGGAAGCGGTAAAACAGGCGGGCATTGAGTCTGATCAAATCGATCAGGTATTGA
TGGGATGCGTCTTAACCGCAGGTCAAGGGCAAGCACCTGCACGTCAAGCAACGTTGAAGGCGGGATTAAGTGTATCTGTC
GCCTGTACCACACTGAATAAAGTCTGTGGCTCAAGTCTACAAAGTATTATTTTTGCAAAAAATTTACTAGAAACTCAACA
AGCACAGGTGGTGATTGCAGGGGGGATGGAAAGCATGTCCAATACACCCTATTTGTTACAGCATGCGCGTGCTGGCTATC
GTATGGGGCATGGTAAAGTGTTGGATCACATGTTTCTAGATGGCCTGGAGGATGCTTACCAACCTGGCCGCCTCATGGGA
ACCTTCGCAGAAGATAGTGCACAACATTATCAGCTAAGTCGTGAACAGCAAGATCATTTTGCAATCACATCTTTGCAACG
AGCACGTGATGCTATTGAAACAGATCAGTTTAAGGCCGAAATTGTAGCTTTAACTGTACATCATAAAGATCAACAACACA
TTATCCAGCAAGATGAACAACCCTTAAAAGCTAAACCTGAAAAAATCTCTCAACTCAAACCAGCATTTAGCAGTAGTGGA
ACAATTACAGCAGCCAATGCGAGTTCAATTTCAGATGGCGCAGCAGCATTGGTTCTGACCAGTAAAAATTATGCACAAAG
CCAAGGGCTTAAGCCTTTAGCTCGTATTGTTGGACAAACATCATACGCTGGTGAACCCCATTTATTTACGACTGCTCCAG
TTTATGCAATTCAGAAACTGATTAAACAATGTGACTGGAACATTGACCAAGTTGATCTATTTGAAATTAATGAAGCTTTT
GCCATTGTACCTATGATTGCAATGCACGAGTTAGGCATTCCACATGCCAAAGTGAATGTTCATGGTGGCGCATGTGCCTT
AGGGCATCCACTGGGTACTTCTGGGGCACGCATTGTGGTGACCCTCATTCATGCACTCAGACAACGTCAACTAAAACGGG
GTATTGCTGCTATTTGTATTGGTGGTGGGGAAGCGTTAGCTCTTGCAATAGAAATTGATTAA

Upstream 100 bases:

>100_bases
GGAGTTTTAAAACAACAGGAAATCTCCCGTATGCAGTTATCGGGTTAAATTTTTGATCATCAGGCAGAGCATTAAGGTAT
TCGTTCTTTAGAGATAGACA

Downstream 100 bases:

>100_bases
ACTCTAGGGACAGAACATGATTTTGACAGAACAACAGCAGCAAATTAGAGAGATGACTCAACAATTTGCTCAAGAAAGAA
TTAAGCCACTCTCGGCAGAG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNNEVVILSAIRTPMGGFLGELKPFSATELGSFAIQEAVKQAGIESDQIDQVLMGCVLTAGQGQAPARQATLKAGLSVSV
ACTTLNKVCGSSLQSIIFAKNLLETQQAQVVIAGGMESMSNTPYLLQHARAGYRMGHGKVLDHMFLDGLEDAYQPGRLMG
TFAEDSAQHYQLSREQQDHFAITSLQRARDAIETDQFKAEIVALTVHHKDQQHIIQQDEQPLKAKPEKISQLKPAFSSSG
TITAANASSISDGAAALVLTSKNYAQSQGLKPLARIVGQTSYAGEPHLFTTAPVYAIQKLIKQCDWNIDQVDLFEINEAF
AIVPMIAMHELGIPHAKVNVHGGACALGHPLGTSGARIVVTLIHALRQRQLKRGIAAICIGGGEALALAIEID

Sequences:

>Translated_393_residues
MNNEVVILSAIRTPMGGFLGELKPFSATELGSFAIQEAVKQAGIESDQIDQVLMGCVLTAGQGQAPARQATLKAGLSVSV
ACTTLNKVCGSSLQSIIFAKNLLETQQAQVVIAGGMESMSNTPYLLQHARAGYRMGHGKVLDHMFLDGLEDAYQPGRLMG
TFAEDSAQHYQLSREQQDHFAITSLQRARDAIETDQFKAEIVALTVHHKDQQHIIQQDEQPLKAKPEKISQLKPAFSSSG
TITAANASSISDGAAALVLTSKNYAQSQGLKPLARIVGQTSYAGEPHLFTTAPVYAIQKLIKQCDWNIDQVDLFEINEAF
AIVPMIAMHELGIPHAKVNVHGGACALGHPLGTSGARIVVTLIHALRQRQLKRGIAAICIGGGEALALAIEID
>Mature_393_residues
MNNEVVILSAIRTPMGGFLGELKPFSATELGSFAIQEAVKQAGIESDQIDQVLMGCVLTAGQGQAPARQATLKAGLSVSV
ACTTLNKVCGSSLQSIIFAKNLLETQQAQVVIAGGMESMSNTPYLLQHARAGYRMGHGKVLDHMFLDGLEDAYQPGRLMG
TFAEDSAQHYQLSREQQDHFAITSLQRARDAIETDQFKAEIVALTVHHKDQQHIIQQDEQPLKAKPEKISQLKPAFSSSG
TITAANASSISDGAAALVLTSKNYAQSQGLKPLARIVGQTSYAGEPHLFTTAPVYAIQKLIKQCDWNIDQVDLFEINEAF
AIVPMIAMHELGIPHAKVNVHGGACALGHPLGTSGARIVVTLIHALRQRQLKRGIAAICIGGGEALALAIEID

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=390, Percent_Identity=44.6153846153846, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=41.7302798982188, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI167614485, Length=394, Percent_Identity=36.0406091370558, Blast_Score=249, Evalue=4e-66,
Organism=Homo sapiens, GI4501853, Length=402, Percent_Identity=32.8358208955224, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=27.1662763466042, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI194353979, Length=399, Percent_Identity=26.5664160401003, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=42.9667519181586, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=47.1649484536082, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1787663, Length=399, Percent_Identity=36.5914786967419, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI48994986, Length=400, Percent_Identity=34.75, Blast_Score=200, Evalue=1e-52,
Organism=Escherichia coli, GI1788683, Length=409, Percent_Identity=26.8948655256724, Blast_Score=132, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI25147385, Length=390, Percent_Identity=40, Blast_Score=287, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI133906874, Length=388, Percent_Identity=38.659793814433, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17535921, Length=392, Percent_Identity=40.3061224489796, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17535917, Length=398, Percent_Identity=32.9145728643216, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17551802, Length=422, Percent_Identity=29.1469194312796, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=399, Percent_Identity=43.859649122807, Blast_Score=308, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6322031, Length=390, Percent_Identity=32.5641025641026, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=43.9897698209719, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=44.1025641025641, Blast_Score=315, Evalue=4e-86,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=34.9489795918367, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=28.8056206088993, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 42079; Mature: 42079

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNEVVILSAIRTPMGGFLGELKPFSATELGSFAIQEAVKQAGIESDQIDQVLMGCVLTA
CCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GQGQAPARQATLKAGLSVSVACTTLNKVCGSSLQSIIFAKNLLETQQAQVVIAGGMESMS
CCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCC
NTPYLLQHARAGYRMGHGKVLDHMFLDGLEDAYQPGRLMGTFAEDSAQHYQLSREQQDHF
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHCCHHHHHHHHHHHHHH
AITSLQRARDAIETDQFKAEIVALTVHHKDQQHIIQQDEQPLKAKPEKISQLKPAFSSSG
HHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCC
TITAANASSISDGAAALVLTSKNYAQSQGLKPLARIVGQTSYAGEPHLFTTAPVYAIQKL
CEEECCCCCCCCCCEEEEEECCCHHHHCCCCHHHHHHCCCCCCCCCCEEECCHHHHHHHH
IKQCDWNIDQVDLFEINEAFAIVPMIAMHELGIPHAKVNVHGGACALGHPLGTSGARIVV
HHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCCCCCHHHHH
TLIHALRQRQLKRGIAAICIGGGEALALAIEID
HHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEC
>Mature Secondary Structure
MNNEVVILSAIRTPMGGFLGELKPFSATELGSFAIQEAVKQAGIESDQIDQVLMGCVLTA
CCCCEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GQGQAPARQATLKAGLSVSVACTTLNKVCGSSLQSIIFAKNLLETQQAQVVIAGGMESMS
CCCCCCHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHCC
NTPYLLQHARAGYRMGHGKVLDHMFLDGLEDAYQPGRLMGTFAEDSAQHYQLSREQQDHF
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHCCHHHHHHHHHHHHHH
AITSLQRARDAIETDQFKAEIVALTVHHKDQQHIIQQDEQPLKAKPEKISQLKPAFSSSG
HHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHCCCCCCCC
TITAANASSISDGAAALVLTSKNYAQSQGLKPLARIVGQTSYAGEPHLFTTAPVYAIQKL
CEEECCCCCCCCCCEEEEEECCCHHHHCCCCHHHHHHCCCCCCCCCCEEECCHHHHHHHH
IKQCDWNIDQVDLFEINEAFAIVPMIAMHELGIPHAKVNVHGGACALGHPLGTSGARIVV
HHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCEEECCCCCCCCCHHHHH
TLIHALRQRQLKRGIAAICIGGGEALALAIEID
HHHHHHHHHHHHCCCEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]